RESUMO
Role overload in the family can be understood as a feeling of inability to complete duties that are the individual's responsibility, and this hardship occurs due to the accumulation of tasks in the family, which can cause discomfort. The aim of this study was to obtain valid evidence of Family Role Overload in Brazilian samples. Six hundred and forty Brazilian workers of both genders took part in the study. Confirmatory factor analyses showed that the Brazilian version remained single-factor and had six items. The multi-group analyses showed configural, metric, and scalar invariance between the groups divided in terms of gender and the existence or absence of children. The scale showed positive correlations with perceived family demands and family-work conflict and a negative correlation with perceived social support in the family. It was therefore concluded that the instrument had psychometric properties that recommend its use in future research.
A sobrecarga de papeis na família pode ser compreendida como sentimento de incapacidade em concluir obrigações que são de responsabilidade do indivíduo, e essa dificuldade acontece devido ao acúmulo de tarefas na família, que podem trazer desconforto. O presente trabalho teve por objetivo obter evidências de validade da Sobrecarga de Papéis na Família em amostras brasileiras. Participaram do estudo 640 trabalhadores brasileiros, de ambos os gêneros. As análises fatoriais confirmatórias evidenciaram que a versão brasileira se manteve unifatorial e com seis itens. As análises multigrupo atestaram a invariância configural, métrica e escalar entre os grupos divididos em termos de gênero e sobre a presença ou ausência de filhos. A escala apresentou correlações positivas com demandas percebidas da família e conflito família-trabalho e correlação negativa com suporte social percebido na família. Concluiu-se, assim, que o instrumento apresentou propriedades psicométricas que recomendam seu uso em investigações futuras.
La sobrecarga de rol en la familia puede ser entendida como un sentimiento de incapacidad para completar las obligaciones que son responsabilidad del individuo, y esta dificultad ocurre debido a la acumulación de tareas en la familia, lo que puede causar malestar. El objetivo de este estudio fue obtener evidencias de la validez de la Escala de Sobrecarga del Rol Familiar en muestras brasileñas. Participaron en el estudio 640 trabajadores brasileños de ambos sexos. Los análisis factoriales confirmatorios mostraron que la versión brasileña se mantuvo monofactorial y con seis ítems. Los análisis multigrupo mostraron invariancia configuracional, métrica y escalar entre los grupos divididos en función del género y de la presencia o ausencia de hijos. La escala mostró correlaciones positivas con las demandas familiares percibidas y el conflicto familia-trabajo y una correlación negativa con el apoyo social percibido en la familia. Por lo tanto, se concluyó que el instrumento posee propiedades psicométricas que recomiendan su uso en futuras investigaciones.
RESUMO
Resumen El modelo de cinco factores de la Escala de Actitud hacia la Estadística (EAE-25) propuesta por Auzmendi (1992), para medir la predisposición de los alumnos hacia los contenidos estadísticos, ha sido cuestionada en distintas investigaciones. El propósito de este estudio fue analizar las propiedades psicométricas de la EAE-25 en una muestra de 291 estudiantes mexicanos de la licenciatura en Psicología. La validez y confiabilidad de la escala fueron examinadas mediante Análisis Factorial Confirmatorio (AFC) y el alfa de Cronbach. Los resultados del AFC muestran indicios parciales de estabilidad, cinco ítems fueron eliminados, pero permanecen los cinco factores originales. La escala así configurada muestra una consistencia interna aceptable, los ítems se relacionan positivamente entre sí en cada factor y cada ítem distingue a los universitarios de acuerdo con su actitud favorable y desfavorable hacia la estadística, así como a los de rendimiento alto de los de rendimiento bajo. Por tal motivo, la escala EAE-20 se presenta como un instrumento válido y confiable, de fácil acceso, implementación e interpretación para evaluar las actitudes hacia la estadística en estudiantes universitarios por parte de profesores e investigadores interesados en el tema.
Abstract The five-factor model of the Attitude Scale towards Statistics (EAE-25) proposed by Auzmendi (1992) to measure the predisposition of students towards statistical content has been questioned in different researches. The purpose of this study was to analyze the psychometric properties of the EAE-25 in a sample of 291 Mexican undergraduate students in Psychology. The validity and reliability of the scale were examine using Confirmatory Factor Analysis (CFA) and Cronbach's alpha. The results of the AFC show partial signs of stability, five items were eliminated, but the five original factors remain. The scale configured in this way shows an acceptable internal consistency, the items are positively related to each other in each factor and each item distinguishes between university students according to their favorable and unfavorable attitude towards statistics, as well as those with high performance from those with low performance. For this reason, the EAE-20 is presented as a valid and reliable instrument, easily accessible, implementation and interpretation to assess the attitudes towards statistics in university students by professors and researchers interested in this topic.
RESUMO
Abstract Precise and accurate measurement of metacognitive phenomena has never been more necessary than in today's fast-paced world in which vast quantities of information are readily available to the learner. The MAI, Jr. (see Sperling et al., 2002) is a widely used, 18-question, self-report measure of metacognitive awareness. However, this measure has not been re-examined for construct validity and internal consistency since its inception in 2002. In this manuscript we report on our findings of a 2-year study in which we worked to validate a shortened version of the MAI, Jr. Over the course of 2 years, 601 students in grades 6-8 participated in our study. In each year, data was examined using exploratory factor analysis with common factor extractions (principal axis factoring [PAF]) and oblique rotations (promax). The results of this study support the validation of a shortened, 7-item, scale. We discuss why shorter measures with appropriate construct validity and internal consistency are preferred.
Resumen La medición precisa y exacta de los fenómenos metacognitivos nunca ha sido más necesaria que en el acelerado mundo actual, en el que el alumno dispone de grandes cantidades de información. El MAI, Jr. (ver Sperling et al., 2002) es una medida de autoinforme de conciencia metacognitiva de 18 preguntas ampliamente utilizada. Sin embargo, esta medida no ha sido reexaminada en cuanto a validez de constructo y consistencia interna desde su inicio en 2002. En este documento se informa sobre los hallazgos de un estudio de 2 años en el que trabajamos para validar una versión abreviada del MAI, Jr. En el transcurso de 2 años, 601 estudiantes de 6.º a 8.º grado participaron en nuestro estudio. En cada año, los datos se examinaron mediante análisis factorial exploratorio con extracciones de factores comunes (factorización del eje principal [PAF]) y rotaciones oblicuas (promax). Los resultados de este estudio respaldan la validación de una escala abreviada de 7 ítems. Se discute por qué se prefieren medidas más cortas con validez de constructo y consistencia interna apropiadas.
RESUMO
Abstract Background: Role stress is linked to reduced work performance, diminished organizational commitment, increased intention to leave a job, and negative physical and mental health effects. Given the significant implications of role stress, researchers have sought to understand and quantify the concept. The Role Conflict and Ambiguity (RCA) scales are widely utilized in job stress research as the predominant measurement tools. They were originally conceptualized as consisting of two independent dimensions: role conflict and role ambiguity. Objective: This study advances the validation research of the RCA scales by exploring its dimensionality through Mokken Scale Analysis (MSA) and Classical Test Theory (CTT). Method: South African school teachers responded to the RCA scales, Maslach Burnout Inventory, and Teaching Satisfaction Scale. Confirmatory Factor Analysis (CFA) and MSA were employed for data analysis. Results: The research determined that a second-order model provided the optimal fit, indicating that role ambiguity and role conflict are subordinate dimensions within the overarching construct of role stress. Conclusion: The findings from the CFA and supplementary bifactor indices reinforce the view that the instrument comprises 13 items, which assess a general dimension of role stress along with two sub-dimensions: role conflict and role ambiguity. Such specificity may lead to more effective strategies to mitigate role-related stress, thereby enhancing overall employee well-being, job satisfaction, and organizational productivity.
Resumen Antecedentes: El estrés de rol está relacionado con la reducción del rendimiento laboral, la disminución del compromiso organizativo, el aumento de la intención de abandonar el trabajo y los efectos negativos sobre la salud física y mental. Dadas las importantes implicaciones del estrés de rol, los investigadores han tratado de comprender y cuantificar este concepto. Las escalas de Conflicto y Ambigüedad de Roles (RCA) se utilizan ampliamente en la investigación del estrés laboral como herramientas de medición predominantes. Al principio, se conceptualizaron como dos dimensiones independientes: conflicto de rol y ambigüedad de rol. Objetivo: Este estudio avanza en la investigación de validación de las escalas RCA, explorando su dimensionalidad mediante el Análisis de Escalas de Mokken (MSA) y la Teoría Clásica de los Test (CTT). Método: Profesores sudafricanos respondieron a las escalas RCA, Maslach Burnout Inventory y Teaching Satisfaction Scale. Se emplearon el Análisis Factorial Confirmatorio (AFC) y el MSA para el análisis de los datos. Resultados: La investigación determinó que un modelo de segundo orden proporcionaba el ajuste óptimo, indicando que la ambigüedad de rol y el conflicto de rol son dimensiones subordinadas dentro del constructo global del estrés de rol. Conclusiones: Los resultados del AFC y los índices bifactoriales suplementarios refuerzan la opinión de que el instrumento consta de 13 ítems, que evalúan una dimensión general de estrés de rol junto con dos subdimensiones: conflicto de rol y ambigüedad de rol. Esta especificidad puede conducir a estrategias más eficaces para mitigar el estrés relacionado con el rol, mejorando así el bienestar general de los empleados, la satisfacción en el trabajo y la productividad de la organización.
RESUMO
Resumen Objetivo: Explorar las dimensiones latentes presentes en los 20 ítems del constructo empatía, medido en docentes de una facultad de Odontología. Metodología: Docentes de odontología, pertenecientes a la Facultad de Odontología de la Universidad Andrés Bello, sede Santiago, constituida por 145 profesores de ambos sexos, en los cuales participaron el 88,27%. El instrumento aplicado es la Escala de Empatía de Jefferson para Profesionales de la Salud. La conformación del modelo de medida de la empatía se realizó mediante el análisis factorial exploratorio. El nivel de significación empleado fue de α<0,005. Resultados: Los resultados generales indican que los niveles de empatía son adecuados. Es posible establecer un modelo de tres factores. Dentro de la dimensión Cuidado Compasivo, perteneciente a la Empatía Emocional, se encuentra un ítem (14), el cual presentó una carga factorial significativa con Toma de Perspectiva con el paciente que pertenece a la Empatía Cognitiva. Conclusiones: Se establece un modelo tridimensional de la empatía en docentes de pregrado con el paciente. Los ítems de cada una de las dimensiones se correlacionaron entre sí, en concordancia con el modelo original de la empatía. Estos hallazgos deben ser corroborados por un análisis factorial confirmatorio.
Abstract Objective: To explore the latent dimensions, present in the 20 items of the empathy construct, measured in teachers of a dental school. Methodology: Dental teachers belonging to the Faculty of Dentistry of the Andrés Bello University, Santiago, consisting of 145 teachers of both sexes, in which 88.27% participated. The instrument applied was the Jefferson Empathy Scale for Health Professionals. Compliance with the model and the measurement of empathy levels was verified by means of exploratory factor analysis. The empathy measurement model was built using exploratory factor analysis. The level of significance used was α<0.005. Results: The overall results indicate that empathy levels are adequate. A three-factor model can be established. Within the Compassionate Care dimension, belonging to Emotional Empathy there is one item (14), which presented a significant correlation with Perspective Taking with the patient belonging to Cognitive Empathy. Conclusions: A three-dimensional model of empathy in undergraduate teachers with the patient is established. The items of each of the dimensions correlated with each other in agreement with the original model of empathy. These findings should be corroborated by a confirmatory factor analysis.
RESUMO
Resumen Introducción: la tuberculosis es una enfermedad infecciosa crónica causada por Mycobacterium tuberculosis, capaz de afectar cualquier órgano o tejido siendo la forma más común la enfermedad pulmonar. Objetivo: identificar los factores que afectan la adherencia al tratamiento antituberculoso en pacientes del Departamento de Boyacá durante los años 2017-2019. Metodología: estudio descriptivo retrospectivo que utilizó como fuentes de información datos registrados en la base del programa departamental de Tuberculosis y SIVIGILA. Se realizó análisis univariado, determinando la distribución de las variables y análisis bivariado en el que se exploró la asociación entre la adherencia al tratamiento de tuberculosis y las variables independientes, se utilizó la prueba de Chi cuadrado. Resultados: se incluyeron 402 pacientes, la adherencia al tratamiento antituberculoso fue 96,5% y la no adherencia de 3,5%. El 66,7% eran de sexo femenino; 55,7% vivián en zona urbana; 66,5% pertenecían al régimen subsidiado; 89,7% mestizos; 91,2% correspondían a otros grupos poblacionales, seguido del 7,5% de población privada de la libertad y 1,3% habitantes de calle. Dentro de las comorbilidades la coinfección con VIH con 4,2% fue la de mayor presencia. Entre las causas de no adherencia al tratamiento fueron desinterés, cambio frecuente de domicilio, contrato finalizado con la EPS y suspensión del tratamiento por toxicidad hepática. El paciente con mayor probabilidad de abandonar el tratamiento pertenece al sexo masculino, al grupo poblacional de migrantes o habitantes de calle y se encuentra afiliado al régimen subsidiado, se encontró una relación estadísticamente significativa entre estas variables y el resultado con la adherencia al tratamiento. Conclusiones: si bien el resultado obtenido en el presente trabajo no es elevado, es importante realizar vigilancia de la adherencia al tratamiento antituberculoso para disminuir el riesgo de complicaciones derivadas de su abandono como mayor mortalidad, desarrollo de resistencia bacteriana y un período de contagiosidad más prolongado.
Abstract Introduction: Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis, capable of affecting any organ or tissue, the most common form being lung disease. Objective: Identify the factors that affect adherence to tuberculosis treatment in patients from the Department of Boyacá during the years 2017-2019. Methodology: Retrospective descriptive study that used as information sources data registered in the database of the departmental Tuberculosis program and SIVIGILA. A univariate analysis was carried out, determining the distribution of the variables and a bivariate analysis in which the association between adherence to tuberculosis treatment and the independent variables was explored, using the Chi-square test. Results: 402 patients were included, adherence to anti-tuberculosis treatment was 96.5% and non-adherence 3.5%. 66.7% were female; 55.7% lived in an urban area; 66.5% belonged to the subsidized regime; 89.7% mestizo; 91.2% corresponded to other population groups, followed by 7.5% of the population deprived of liberty and 1.3% homeless. Within the comorbidities, coinfection with HIV 4.2% was the one with the greatest presence. Among the causes of non-adherence to treatment were lack of interest; frequent change of address; Terminated contract with the EPS and suspension of treatment due to liver toxicity. The patient with the highest probability of abandoning treatment belongs to the male sex, to the population group of migrants or street dwellers and is affiliated with the subsidized regime, a statistically significant relationship was found between these variables and the result with adherence to treatment. Conclusions: Although the result obtained in this study is not high, it is important to monitor adherence to tuberculosis treatment to reduce the risk of complications derived from its abandonment, such as increased mortality, development of bacterial resistance, and a longer period of contagiousness.
RESUMO
Gallibacterium anatis is a member of the Pasteurellaceae family and is an opportunistic pathogen that causes gallibacteriosis in chickens. Stress plays a relevant role in promoting the development of pathogenicity in G. anatis. Epinephrine (E) and norepinephrine (NE) are relevant to stress; however, their effects on G. anatis have not been elucidated. In this work, we evaluated the effects of E and NE on the growth, biofilm formation, expression of adhesins, and proteases of two G. anatis strains, namely, the hemolytic 12656-12 and the nonhemolytic F149T biovars. E (10 µM/mL) and NE (30 and 50 µM/mL) increased the growth of G. anatis 12656-12 by 20 % and 25 %, respectively. E did not affect the growth of F149T, whereas 40 µM/mL NE decreased bacterial growth by 25 %. E and NE at a dose of 30-50 µM/mL upregulated five fibrinogen adhesins in the 12565-12 strain, whereas no effect was observed in the F149T strain. NE increased proteolytic activity in both strains, whereas E diminished proteolytic activity in the 12656-12 strain. E and NE reduced biofilm formation (30 %) and increased Congo red binding (15 %) in both strains. QseBC is the E and NE two-component detection system most common in bacteria. The qseC gene, which is the E and NE receptor in bacteria, was identified in the genomic DNA of the 12565-12 and F149TG. anatis strains via PCR amplification. Our results suggest that QseC can detect host changes in E and NE concentrations and that catecholamines can modulate the expression of several virulence factors in G. anatis.
Assuntos
Biofilmes , Galinhas , Epinefrina , Regulação Bacteriana da Expressão Gênica , Norepinefrina , Pasteurellaceae , Fatores de Virulência , Fatores de Virulência/genética , Fatores de Virulência/metabolismo , Norepinefrina/farmacologia , Norepinefrina/metabolismo , Epinefrina/farmacologia , Biofilmes/crescimento & desenvolvimento , Biofilmes/efeitos dos fármacos , Pasteurellaceae/genética , Pasteurellaceae/patogenicidade , Pasteurellaceae/efeitos dos fármacos , Pasteurellaceae/metabolismo , Animais , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Adesinas Bacterianas/genética , Adesinas Bacterianas/metabolismo , Peptídeo Hidrolases/metabolismo , Peptídeo Hidrolases/genética , Doenças das Aves Domésticas/microbiologia , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Infecções por Pasteurellaceae/microbiologia , Infecções por Pasteurellaceae/veterináriaRESUMO
OBJECTIVES: This study investigated altmetrics, citations, and field-normalized impact of dental articles reporting randomized clinical trials (RCTs) published within a one-year period. METHODS: Data were collected in 2024 from PubMed-indexed RCTs published in 2019. Dependent variables included Altmetric Attention Scores (AAS), PlumX citations, and Field-Weighted Citation Impact (FWCI). Independent variables encompassed article-, author-, and journal-related variables. Adjusted quasi-Poisson regression models were used to assess associations. Point-biserial correlation evaluated the relationship between Journal Impact Factor (JIF) and selected reporting variables. RESULTS: A total of 653 RCTs were included, with periodontology, implantology, and oral and maxillofacial surgery comprising 50.4 % of the sample. Only 28.6 % of the articles reported CONSORT use, 49.6 % pre-registered their protocol, and 68.8 % reported a sample size calculation. Most articles (63.6 %) reported no conflicts of interest, with unclear sponsorship being the most frequent (34.6 %). Regression analyses revealed significant associations for AAS, PlumX citations, and FWCI with various factors. JIF increased AAS by 17 % per unit, PlumX citations by 13 %, and FWCI by 6 %. Protocol pre-registration boosted AAS by 132 %, while mixed or no sponsorship increased PlumX citations by up to 47 %. First author H-index increased PlumX citations and FWCI by 1 % per unit, while first author continent impacted AAS, citations, and FWCI. Weak positive correlations between JIF and both protocol pre-registration and CONSORT use were observed. No significant differences were observed across different dental fields for any metric. CONCLUSION: An interplay among article-, author-, and journal-related variables collectively influenced the online attention, citations, and impact of dental RCT articles. CLINICAL SIGNIFICANCE: Understanding the factors that influence the visibility and impact of dental RCTs can guide researchers in improving the design, reporting, and dissemination of their studies, ultimately enhancing the quality and reach of dental research.
RESUMO
BACKGROUND: Gouty arthritis is a metabolic disease characterized by the deposition of monosodium urate crystals in the joints, which triggers the release of interleukin-1ß (IL-ß) by activating the NLRP3 inflammasome. Hypoxia-inducible factor-1α (HIF-1α) is a transcription factor involved in IL-ß production and as a regulator of NLRP3. OBJECTIVES: The aims were to analyze the association of HIF1A rs11549465, rs11549467, and rs2057482 variants in patients with gouty arthritis, and to evaluate the correlation between urate and HIF-1α levels according to the associated genotypes. METHODS: Cases and controls were genotyped using TaqMan probes, and urate and HIF-1α levels were quantified. Data were analyzed using SPSS v21 software and P-values < 0.05 were considered statistically significant. RESULTS: Urate and HIF-1α levels were higher in patients than in controls (P < 0.05). Under the three inheritance models (codominant, dominant, and recessive), the AA genotype of the rs11549467 variant was associated with gout risk (OR = 5.74, P = 0.009, OR = 3.33, P = 0.024, and OR = 9.09, P = 0.003, respectively). There were significant differences in the distribution of serum levels of both HIF-1α (P < 0.0001) and urate (P = 0.016) according to the genotypes of the rs11549467 variant. CONCLUSION: These results suggest that the HIF1A rs11549467 variant may play a key role in the pathogenesis of gouty arthritis. Key Points ⢠The pathogenesis of gouty arthritis involves the HIF1A gene. ⢠In patients with gout, the AA genotype of the rs11549467 (HIF1A) variant is associated with increased serum levels of urate and HIF-1α. ⢠HIF-1α is involved in the regulation of IL-1ß and NLRP3.
Assuntos
Artrite Gotosa , Genótipo , Subunidade alfa do Fator 1 Induzível por Hipóxia , Polimorfismo de Nucleotídeo Único , Ácido Úrico , Humanos , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Subunidade alfa do Fator 1 Induzível por Hipóxia/sangue , Ácido Úrico/sangue , Masculino , Artrite Gotosa/genética , Artrite Gotosa/sangue , Feminino , Pessoa de Meia-Idade , Adulto , Estudos de Casos e Controles , Predisposição Genética para Doença , Proteína 3 que Contém Domínio de Pirina da Família NLR/genéticaRESUMO
BACKGROUND: The molecular system of receptor activator of nuclear factor kappa-ß (RANK) and its ligand (RANKL) plays a role in a variety of physiological and pathological processes. These encompass the regulation of bone metabolism, mammary gland development, immune function, as well as their involvement and tumorigenesis. Nevertheless, limited knowledge exists regarding their function within the tumor microenvironment. METHODS AND RESULTS: We explored the significance of RANK expression in cancer-associated fibroblasts (CAFs) as a prognostic biomarker in early breast cancer patients (BCPs) by immunohistochemistry. Results reveal a significant correlation between high RANK expression in CAFs and an increased risk of metastasis (p= 0.006), shorter metastasis-free survival (MFS) [p= 0.007, OR (95%CI) = 2.290 (1.259-4.156)], and lower overall survival (OS) [p= 0.004, OR (95%CI) = 2.469 (1.343-4.541)]. Upon analyzing the phenotype of CD34(-) CAFs isolated from primary tumors in BCPs, we observed co-expression of RANK with CD105 marker by immunofluorescence and flow cytometry, characteristic of mesenchymal stem/stromal cells (MSCs), suggesting the possible cellular origin. Also RANKL-RANK system increase the OCT-4, SOX-2 and DKK-1 (dickkopf 1) gene expression in CD34(-) CAFs by RT-PCR. Moreover, this system plays a crucial role in the migration of these CD34(-) CAFs. CONCLUSIONS: These results support the clinical relevance of RANK in CAFs and propose its potential as a future therapeutic target in the treatment of early BCPs.
Assuntos
Biomarcadores Tumorais , Neoplasias da Mama , Fibroblastos Associados a Câncer , Estadiamento de Neoplasias , Receptor Ativador de Fator Nuclear kappa-B , Humanos , Feminino , Neoplasias da Mama/patologia , Neoplasias da Mama/metabolismo , Neoplasias da Mama/mortalidade , Neoplasias da Mama/genética , Fibroblastos Associados a Câncer/metabolismo , Fibroblastos Associados a Câncer/patologia , Receptor Ativador de Fator Nuclear kappa-B/metabolismo , Prognóstico , Biomarcadores Tumorais/metabolismo , Biomarcadores Tumorais/genética , Metástase Neoplásica , Pessoa de Meia-Idade , Microambiente Tumoral , Ligante RANK/metabolismo , Ligante RANK/genética , Adulto , Idoso , Linhagem Celular TumoralRESUMO
Objective: Analyse the psychometric properties of the Portuguese version of the PAFAS (Parenting and Family Adjustment Scales) parenting scale, using data from two large Brazilian birth cohorts. Methods: The original PAFAS parenting scale, which consists of 18 items (parental inconsistency 5 items, coercive parenting 5 items, positive encouragement 3 items, and parent-child relationship 5 items) was applied in two Brazilian birth cohorts in Pelotas (ages 4 [n = 4010] and 6-7 [n = 3867]) and Rio Grande (age 3 [n = 992]). Confirmatory factor analysis was conducted, and internal consistency assessed, as well as construct validity in relation to maternal depression measured on the Edinburgh Postnatal Depression Scale. Results: The model with a structure of 4 subscales showed that the global scale of parenting on the PAFAS had a good fit, but certain items did not fit well on sub-scales and were removed (2 items from parental inconsistency, 1 from coercive parenting, and 1 from positive encouragement). The original form of the parent-child relationship sub-scale was maintained. Considering the total PAFAS parenting score, we found that mothers with maternal depression had a higher likelihood of more problematic parental practices than mothers without depression. Conclusions: A revised 14-item PAFAS parenting scale has good psychometric properties and we encourage its use in Brazilian populations.
RESUMO
INTRODUCTION: Despite its crucial role in Epidermal Growth Factor Receptor (EGFR) activation, and the resulting impact on the health-disease process, epidermal growth factor (EGF) is an underexplored molecule in relation to how its serum concentrations relate to other analytes and clinical variables in pathological contexts. OBJECTIVE: To clarify the possible correlation between EGF and clinical and analytical variables in the context of COVID-19. METHODS: Cross-sectional observational and analytical study, in patients with virological and clinical diagnosis of COVID-19, selected by simple random sampling, admitted between August and September 2021. UMELISA-EGF commercial kits were used. RESULTS: Differences in overall EGF values were observed between groups (566.04 vs. 910.53 pg/ml, p = .0430). In COVID-19 patients, no notable correlations were observed for neutrophil, platelet, triglyceride or liver enzyme values (p > .05). Significant correlations were observed with the neutrophil-lymphocyte indicator (r = 0.4711, p = .0128) as well as with the platelet-lymphocyte index (r = 0.4553, p = .0155). Statistical results of multivariate regression analysis suggest NLR (ß = .2232, p = .0353) and PLR (ß = .2117, p = .0411) are predictors of inflammation in patients with COVID-19. CONCLUSIONS: Serum EGF concentrations in COVID-19 correlate positively with prognostic inflammatory markers of severity and could presumably act as an independent risk factor for the development of inflammation in response to new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
Assuntos
COVID-19 , Fator de Crescimento Epidérmico , Inflamação , SARS-CoV-2 , Humanos , COVID-19/sangue , COVID-19/diagnóstico , Fator de Crescimento Epidérmico/sangue , Masculino , Feminino , Estudos Transversais , Pessoa de Meia-Idade , Idoso , Inflamação/sangue , Adulto , Biomarcadores/sangue , Neutrófilos/imunologiaRESUMO
[This corrects the article DOI: 10.3389/fpsyg.2023.1194294.].
RESUMO
Background: Li-Fraumeni syndrome (LFS) is a rare hereditary disorder caused by mutations in the tumor protein p53 (TP53). It causes a predisposition for the development of multiple malignancies, primarily including breast cancers, sarcomas, and central nervous system tumors. There are a few cases reported in the literature of patients with LFS presenting with an epidermal growth factor receptor (EGFR) mutated lung cancer. Still, it has been suggested that there may be an association between the TP53 pathogenic variant and lung cancer with EGFR mutation in somatic cells. Case Description: A 47-year-old non-smoker woman with LFS with a history of multiple tumors, including bilateral breast cancer, pecoma, and sarcoma. In one of her computed tomography, a lesion in the lingula of the lung was detected. It was biopsied, which diagnosed lung adenocarcinoma, and genetic studies detected an EGFR exon 19 deletion. She was treated with a left inferior lobectomy, followed by pemetrexed and cisplatin. Conclusions: The association between TP53 and lung cancer with EGFR mutation has been suggested in case reports. Studies in lung cancer cell lines have shown a link between TP53 mutation and EGFR overexpression. Nonetheless, as more cases are reported, further research is needed to comprehend the interrelation between these two pathologies and the risk posed by LFS to the emergence of EGFR-mutated lung cancer.
RESUMO
Objetivo: Evaluar el valor predictivo negativo de la ratio antigénica y conocer su rentabilidad para descartar preeclampsia precoz en pacientes de alto riesgo de desarrollarla, con profilaxis de ácido acetilsalicílico. Métodos: Se realizó un estudio descriptivo transversal que recogió a las gestantes con cribado de preeclampsia precoz de alto riesgo (384 gestantes) en el Hospital Santa Lucía durante el año 2021, para lo que se usó test Elecsys® tabulado a un riesgo mayor a 1/150 en primer trimestre, y que tomaran ácido acetilsalicílico antes de la semana 16, quedando en 368 gestantes vistas en las semanas 20, 26, 31 y 36. Se realizó biometría, ratio angiogénica y doppler. Resultados: La incidencia de preeclampsia precoz en la población fue 4 casos (incidencia 1,08 %). Son significativos por su alto valor predictivo negativo del 100 % de preeclampsia precoz: la ratio angiogénica mayor a 38 en la semana 26 y el doppler de las uterinas en semana 20 y 26. Conclusión: En gestaciones con cribado de alto riesgo de preeclampsia que tomen ácido acetilsalicílico, una ratio angiogénica menor a 38 en la semana 26, además de un doppler uterino normal en semana 20 y 26 permite reducir el seguimiento gestacional(AU)
Objective: Our main objective was to evaluate the negative predictive value of the angiogenic ratio and to know its profitability to rule out early preeclampsia in patients at high risk of early preeclampsia with acetylsalicylic acid prophylaxis. Methods: A cross-sectional descriptive study was carried out that included pregnant women with high-risk early preeclampsia screening (384 pregnant women) at the Santa Lucía Hospital during the year 2021, for which the Elecsys® test tabulated at a risk >1/ was used. 150 in the first trimester, and who take acetylsalicylic acid before week 16, leaving 368 pregnant women seen in weeks 20, 26, 31 and 36, with biometry, angiogenic ratio and Doppler performed. Results: The incidence of early preeclampsia in the population was 4 cases (incidence 1.08%). They are significant due to their high negative predictive value of 100% of early preeclampsia: Angiogenic ratio > 38 in week 26, uterine Doppler in weeks 20 and 26. Conclusion: Pregnancies with high risk screening for preeclampsia who take acid acetylsalicylic acid, an angiogenic ratio < 38 at week 26 in addition to a normal uterine Doppler at weeks 20 and 26 allows for reduced gestational follow-up(AU)
Assuntos
Humanos , Feminino , Gravidez , Pré-Eclâmpsia , Aspirina , Programas de Rastreamento , Valor Preditivo dos Testes , Proteínas Angiogênicas , Placenta , Primeiro Trimestre da Gravidez , Fator de Crescimento Placentário , AntígenosRESUMO
OBJECTIVES: This study aimed to explore the correlation between Fibroblast Growth Factor-23 (FGF23) levels and Cerebral Infarction (CI), and to determine whether there is a significant relationship between FGF23 and the occurrence and severity of CI. METHODS: The study categorized Cerebral Infarction (CI) patients into severe and mild stenosis groups based on vertebrobasilar artery stenosis, using Digital Subtraction Angiography (DSA) and Magnetic Resonance Imaging (MRI). The study compared the levels of Fibroblast Growth Factor-23 (FGF23) in the serum of CI patients and healthy controls using a t-test and evaluated the diagnostic effectiveness of serum FGF23 using a Receiver Operating Characteristic (ROC) curve. Additionally, the study analyzed the correlation between FGF23 levels and CI severity after treatment using the National Institute of Health Stroke Scale score. RESULTS: The study found a significant increase in serum Fibroblast Growth Factor-23 (FGF23) levels in patients with Cerebral Infarction (CI) compared to healthy volunteers, (p < 0.001). A higher serum FGF23 level was observed in the severe stenosis group than in the mild stenosis group (p < 0.001). Furthermore, the study showed that a high FGF23 level at admission was significantly related to more severe symptoms of CI as indicated by the National Institute of Health Stroke Scale (NIHSS) score on the 7th day after treatment (p < 0.001). CONCLUSIONS: This study discovered a correlation between Fibroblast Growth Factor-23 (FGF23) levels, vertebrobasilar artery stenosis, and short-term prognosis in patients who had recently experienced acute Cerebral Infarction (CI).
Assuntos
Infarto Cerebral , Fator de Crescimento de Fibroblastos 23 , Fatores de Crescimento de Fibroblastos , Índice de Gravidade de Doença , Insuficiência Vertebrobasilar , Humanos , Insuficiência Vertebrobasilar/sangue , Insuficiência Vertebrobasilar/diagnóstico por imagem , Infarto Cerebral/sangue , Infarto Cerebral/diagnóstico por imagem , Feminino , Masculino , Fatores de Crescimento de Fibroblastos/sangue , Pessoa de Meia-Idade , Idoso , Estudos de Casos e Controles , Imageamento por Ressonância Magnética , Angiografia Digital , Biomarcadores/sangue , Curva ROC , Adulto , Valores de ReferênciaRESUMO
BACKGROUND: Common bean (Phaseolus vulgaris) is one of the main nutritional resources in the world, and a low environmental impact source of protein. However, the majority of its cultivation areas are affected by drought and this scenario is only expected to worsen with climate change. Stomatal closure is one of the most important plant responses to drought and the MYB60 transcription factor is among the key elements regulating stomatal aperture. If targeting and mutating the MYB60 gene of common bean would be a valuable strategy to establish more drought-tolerant beans was therefore investigated. RESULTS: The MYB60 gene of common bean, with orthology to the Arabidopsis AtMYB60 gene, was found to have conserved regions with MYB60 typical motifs and architecture. Stomata-specific expression of PvMYB60 was further confirmed by q-RT PCR on organs containing stomata, and stomata-enriched leaf fractions. Further, function of PvMYB60 in promoting stomata aperture was confirmed by complementing the defective phenotype of a previously described Arabidopsis myb60-1 mutant. CONCLUSIONS: Our study finally points PvMYB60 as a potential target for obtaining more drought-tolerant common beans in the present context of climate change which would further greatly contribute to food security particularly in drought-prone countries.
Assuntos
Mudança Climática , Resistência à Seca , Phaseolus , Arabidopsis/genética , Arabidopsis/fisiologia , Resistência à Seca/genética , Regulação da Expressão Gênica de Plantas/genética , Phaseolus/genética , Phaseolus/fisiologia , Proteínas de Plantas/genética , Estômatos de Plantas/genética , Estômatos de Plantas/fisiologia , Fatores de Transcrição/genéticaRESUMO
The oil of Carapa guianensis showed leishmanicidal activity, with its activity being related to limonoids, but fatty acids are the major constituents of this oil. The present study evaluated the physicochemical, pharmacokinetic, and toxicity profiles of limonoids and fatty acids already identified in the species. Based on these results, 2 limonoids (methyl angosinlate, 6-OH-methyl angosinlate) and 2 fatty acids (arachidic acid; myristic acid) were selected for the prediction of possible targets and molecular docking. Included in this study were: Gedunin, 6α-acetoxygedunin, Methyl angosenlato, 7-deacetoxy-7-oxogedunin, Andirobin, 6-hydroxy-angolensate methyl, 17ß-hydroxyazadiradione, 1,2-dihydro-3ß-hydroxy-7-deacetoxy-7-oxogedunin, xyllocensin k, 11beta-Hydroxygedunin, 6α,11-11ß-diacetoxygedunin, Oleic Acid, Palmitic Acid, Stearic Acid, Arachidic Acid, Myristic Acid, Palmitoleic Acid, Linoleic Acid, Linolenic Acid, and Beenic Acid. Regarding physicochemical aspects, fatty acids violated LogP, and only limonoid 11 violated Lipinski's rule. A common pharmacokinetic aspect was that all molecules were well absorbed in the intestine and inhibited CYP. All compounds showed toxicity in some model, with fatty acids being mutagenic and carcinogenic, and limonoids not being mutagenic and carcinogenic at least for rats. In in vivo models, fatty acids were less toxic. Molecular dockings were performed on COX-2 steroids (15 and 16) and hypoxia-inducible factor 1 alpha for limonoids (3,6), with this target being essential for the intracellular development of leishmania. Limonoids 3 and 6 appear to be promising as leishmanicidal agents, and fatty acids are promising as wound healers.
RESUMO
Graphene-based surface plasmon resonance (SPR) biosensors have emerged as a promising technology for the highly sensitive and accurate detection of biomolecules. This study presents a comprehensive theoretical analysis of graphene-based SPR biosensors, focusing on configurations with single and bimetallic metallic layers. In this study, we investigated the impact of various metallic substrates, including gold and silver, and the number of graphene layers on key performance metrics: sensitivity of detection, detection accuracy, and quality factor. Our findings reveal that configurations with graphene first supported on gold exhibit superior performance, with sensitivity of detection enhancements up to 30% for ten graphene layers. In contrast, silver-supported configurations, while demonstrating high sensitivity, face challenges in maintaining detection accuracy. Additionally, reducing the thickness of metallic layers by 30% optimizes light coupling and enhances sensor performance. These insights highlight the significant potential of graphene-based SPR biosensors in achieving high sensitivity of detection and reliability, paving the way for their application in diverse biosensing technologies. Our findings pretend to motivate future research focusing on optimizing metallic layer thickness, improving the stability of silver-supported configurations, and experimentally validating the theoretical findings to further advance the development of high-performance SPR biosensors.
Assuntos
Técnicas Biossensoriais , Ouro , Grafite , Prata , Ressonância de Plasmônio de Superfície , Grafite/química , Ressonância de Plasmônio de Superfície/métodos , Prata/química , Técnicas Biossensoriais/métodos , Técnicas Biossensoriais/instrumentação , Ouro/químicaRESUMO
BACKGROUND: Doege-Potter syndrome is a rare paraneoplastic phenomenon associated with solitary fibrous tumors of the pleura (SFTPs). It is characterized by the presence of severe, sustained, and treatment-refractory hypoglycemia. Hypoglycaemia, which may be the sole symptom at disease onset, is mediated by the secretion of high-molecular-weight insulin-like growth factor (IGF-2). Most tumors exhibit benign behavior, with a 100% survival rate at 5 years. However, 10% of these tumors may display aggressive behavior with local or metastatic recurrence. We present a clinical case of a patient with a benign solitary fibrous tumor of the pleura who presented with symptomatic hypoglycemia and required pulmonary and pleural surgical resection to control the paraneoplastic phenomenon. CASE PRESENTATION: A Hispanic 46-year-old man presented with a 15-day history of transient alterations in consciousness worsened by fasting. The relevant medical history included obstructive sleep apnea treated with continuous positive air pressure (CPAP) and previous smoking. In-hospital studies revealed noninsulinemic hypoglycemia and a benign SFTP. Complete surgical resection was performed while the patient received dextrose fluids and corticosteroids perioperatively for hypoglycemia. Subsequently, the hypoglycemia resolved, and the patient was followed-up without disease recurrence. CONCLUSION: Doege-Potter syndrome is challenging to recognize. However, effective treatment can be achieved with a high survival rate. Raising awareness among healthcare professionals about the recognition of this paraneoplasic syndrome patients will improve diagnostic suspicion, biochemical confirmation, the development of diagnostic and therapeutic guidelines, and the creation of predictive indices for aggressive presentations requiring closer monitoring.