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The objectives of this study are (a) to determine the background concentration of As, Cd, Cr, Hg and Pb in Arica commune; (b) to determine the degree of soil contamination in Arica city using environmental indices and (c) to evaluate the human health risk of these potentially toxic elements. In the rural area of Arica commune, 169 samples were taken and 283 samples were taken in the urban area of Arica city. Total concentrations of Cd, Pb and Cr were determined by EPA 3052 and EPA 6010 C. Mercury was determined by EPA 7473. Arsenic was determined by EPA 7061A. The available concentrations of As and Cr were determined by dilute hydrochloric acid and EPA 6010C. Environmental indices were applied for pollution and US EPA model was used to evaluate human health risk. Background concentrations were As 18.2, Cd 1.12, Cr 73.2, Hg 0.02 and Pb 11.8 mg kg-1, respectively. Environmental indices show that soil samples are located between slightly contaminated to extremely contaminated. Human health risk analysis shows that children have higher levels of risk than adults. The analysis with available concentrations of As and Cr shows no carcinogenic risk for adults and children, but 81% and 98% of the samples were between 10-6 and 10-4, that means intermediate risk.

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Some of the major impacts of climate change are expected in regions where drought stress is already an issue. Grain legumes are generally drought susceptible. However, tepary bean and its wild relatives within Phaseolus acutifolius or P. parvifolius are from arid areas between Mexico and the United States. Therefore, we hypothesize that these bean accessions have diversity signals indicative of adaptation to drought at key candidate genes such as: Asr2, Dreb2B, and ERECTA. By sequencing alleles of these genes and comparing to estimates of drought tolerance indices from climate data for the collection site of geo-referenced, tepary bean accessions, we determined the genotype x environmental association (GEA) of each gene. Diversity analysis found that cultivated and wild P. acutifolius were intermingled with var. tenuifolius and P. parvifolius, signifying that allele diversity was ample in the wild and cultivated clade over a broad sense (sensu lato) evaluation. Genes Dreb2B and ERECTA harbored signatures of directional selection, represented by six SNPs correlated with the environmental drought indices. This suggests that wild tepary bean is a reservoir of novel alleles at genes for drought tolerance, as expected for a species that originated in arid environments. Our study corroborated that candidate gene approach was effective for marker validation across a broad genetic base of wild tepary accessions.

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The frequent environment-unfriendly treatments of agro-industrial bio-wastes cause severe pollution through air pollution and through residual effluents and hazardous solid waste. These bio-wastes can contain phenolic compounds, forms of phenolic acids and flavonoids in plants. They are however the most abundant class of many phytochemicals and have been given great interest due to their health advantage and high economic value. An interesting upgrading of these bio-wastes may consist in obtaining a concentrated extract of phenolic compounds using no-toxic solvents, hence protecting the environment and human health. In this work, different alternatives of the extraction process were evaluated using an exergetic analysis. The energy and water consumptions, CO2 emissions, exergetic yield, wasted and destroyed exergy were calculated. It was found that several alternatives for recycle streams were convenient (streams with higher chemical exergy were not discharged into the environment). The energy and water consumption for the best alternative (ethanol-water ratio 1/1 including recycle stream, named E-W 1/1 Rec) were 567 MJ/h and 105 kg/h, respectively and the CO2 emission was 105 kg/h. The calculated exergy destruction indicated that the evaporation and distillation stages may be optimized towards a more sustainable operation. It is not advisable to dry the bio-waste if it will be immediately processed once generated.

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Genome-environment associations (GEAs) are a powerful strategy for the study of adaptive traits in wild plant populations, yet they still lack behind in the use of modern statistical methods as the ones suggested for genome-wide association studies (GWASs). In order to bridge this gap, we couple GEA with last-generation GWAS algorithms in common bean to identify novel sources of heat tolerance across naturally heterogeneous ecosystems. Common bean (Phaseolus vulgaris L.) is the most important legume for human consumption, and breeding it for resistance to heat stress is key because annual increases in atmospheric temperature are causing decreases in yield of up to 9% for every 1°C. A total of 78 geo-referenced wild accessions, spanning the two gene pools of common bean, were genotyped by sequencing (GBS), leading to the discovery of 23,373 single-nucleotide polymorphism (SNP) markers. Three indices of heat stress were developed for each accession and inputted in last-generation algorithms (i.e. SUPER, FarmCPU, and BLINK) to identify putative associated loci with the environmental heterogeneity in heat stress. Best-fit models revealed 120 significantly associated alleles distributed in all 11 common bean chromosomes. Flanking candidate genes were identified using 1-kb genomic windows centered in each associated SNP marker. Some of these genes were directly linked to heat-responsive pathways, such as the activation of heat shock proteins (MED23, MED25, HSFB1, HSP40, and HSP20). We also found protein domains related to thermostability in plants such as S1 and Zinc finger A20 and AN1. Other genes were related to biological processes that may correlate with plant tolerance to high temperature, such as time to flowering (MED25, MBD9, and PAP), germination and seedling development (Pkinase_Tyr, Ankyrin-B, and Family Glicosil-hydrolase), cell wall stability (GAE6), and signaling pathway of abiotic stress via abscisic acid (histone-like transcription factors NFYB and phospholipase C) and auxin (Auxin response factor and AUX_IAA). This work offers putative associated loci for marker-assisted and genomic selection for heat tolerance in common bean. It also demonstrates that it is feasible to identify genome-wide environmental associations with modest sample sizes by using a combination of various carefully chosen environmental indices and last-generation GWAS algorithms.

RESUMO

Objetivou-se avaliar o efeito do tipo de ordenha e de ambiente na qualidade do leite cru por meio da identificação da Contagem de Células Somáticas - CCS nas amostras obtidas das propriedades rurais da mesorregião do sul goiano e correlacioná-la com diferentes tipos de ordenha, bem como com os componentes do leite e o período do ano. A pesquisa desenvolveu-se em 229 fazendas associadas à Cooperativa de Catalão, divididas por tipo de ordenha: manual e mecânica. Em cada propriedade foi coletada amostra mensal no tanque de expansão, no total de 8244 amostras. A ordenha mecânica obteve maior número de amostras com CCS > 500.000 células/mL. Existiu correlação positiva entre CCS e teor de gordura e sólidos totais - ST, porém houve uma correlação negativa entre CCS e teores de proteína e lactose. No período da chuva tem-se uma elevação de CCS se comparado ao período da seca, e com a elevação do Índice de Temperatura e Umidade - ITU ocorreu uma redução nos teores de gordura e ST. Pode-se concluir que a ordenha manual mostrou-se mais viável para obtenção do leite com parâmetros de CCS exigidos pela legislação e que CCS e componentes do leite sofrem interferência da época do ano.

The objective of this study was to evaluate the effect of the type of milking and the environment on raw milk quality by identification of Somatic Cells Count - CCS in the samples obtained from farms in the mesoregion of south goiano and correlate it with the different types of milk, milk components, and time of year. The research was developed in 229 farms associated with the Catalão Cooperative, divided for types of milking: manual and mechanical. A monthly sample was collected in each property in the expansion tank, totaling 8244 samples. Mechanical milking presented the highest number of samples with CCS > 500.000 cells / mL. There was a positive correlation between CCS and fat and total solids - TS, but there was a negative correlation between CCS and protein and lactose. During the rainy season CCS increased compared to dry season. Also, the rise of temperature and humidity index - THI caused a reduction in fat and TS. In conclusion, hand milking was more feasible to obtain the milk CCS parameters required by the legislation and CCS and milk components suffer interference from the time of the year.

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RESUMO

Objetivou-se avaliar o efeito do tipo de ordenha e de ambiente na qualidade do leite cru por meio da identificação da Contagem de Células Somáticas - CCS nas amostras obtidas das propriedades rurais da mesorregião do sul goiano e correlacioná-la com diferentes tipos de ordenha, bem como com os componentes do leite e o período do ano. A pesquisa desenvolveu-se em 229 fazendas associadas à Cooperativa de Catalão, divididas por tipo de ordenha: manual e mecânica. Em cada propriedade foi coletada amostra mensal no tanque de expansão, no total de 8244 amostras. A ordenha mecânica obteve maior número de amostras com CCS > 500.000 células/mL. Existiu correlação positiva entre CCS e teor de gordura e sólidos totais - ST, porém houve uma correlação negativa entre CCS e teores de proteína e lactose. No período da chuva tem-se uma elevação de CCS se comparado ao período da seca, e com a elevação do Índice de Temperatura e Umidade - ITU ocorreu uma redução nos teores de gordura e ST. Pode-se concluir que a ordenha manual mostrou-se mais viável para obtenção do leite com parâmetros de CCS exigidos pela legislação e que CCS e componentes do leite sofrem interferência da época do ano.(AU)

The objective of this study was to evaluate the effect of the type of milking and the environment on raw milk quality by identification of Somatic Cells Count - CCS in the samples obtained from farms in the mesoregion of south goiano and correlate it with the different types of milk, milk components, and time of year. The research was developed in 229 farms associated with the Catalão Cooperative, divided for types of milking: manual and mechanical. A monthly sample was collected in each property in the expansion tank, totaling 8244 samples. Mechanical milking presented the highest number of samples with CCS > 500.000 cells / mL. There was a positive correlation between CCS and fat and total solids - TS, but there was a negative correlation between CCS and protein and lactose. During the rainy season CCS increased compared to dry season. Also, the rise of temperature and humidity index - THI caused a reduction in fat and TS. In conclusion, hand milking was more feasible to obtain the milk CCS parameters required by the legislation and CCS and milk components suffer interference from the time of the year.(AU)

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A pesquisa teve como objetivo avaliar a influência do sistema de resfriamento adiabático evaporativo (SRAE) sobre as variáveis comportamentais de novilhas leiteiras da raça Holandesa mantidas em confinamento. Foram utilizadas 12 novilhas, distribuídas em três tratamentos: 1) ventilação e SRAE por nebulização quando a temperatura atingia 25°C a qualquer umidade; 2) ventilação e SRAE por nebulização quando a temperatura atingia 25°C com umidade relativa menor ou igual a 70% e, 3 sem sistema de resfriamento. O delineamento experimental consistiu em um retângulo latino repetido duas vezes. As variáveis ambientais (temperatura do bulbo seco, temperatura do globo negro e umidade relativa foram coletadas diariamente, num intervalo de 15 minutos, através de dataloggers, durante todo o experimento. Na análise comportamental foram observadas as posturas (em pé e deitada), as atividades realizadas pelos animais e os locais em que se encontravam na baia, em intervalos de 15 minutos. Contudo, observou-se que não houve diferença nas variáveis comportamentais dos animais entre os tratamentos. (AU)

The research aimed to evaluate the influence of evaporative cooling system (AECS) on the behavioral variables of Holstein dairy heifers kept in confinement. Twelve heifers were used, divided into three treatments: 1) ventilation and fogging by AECS when the temperature reached 25°C at any humidity, 2) ventilation and fogging by AECS when the temperature reached 25°C with relative humidity less than or equal to 70% and, 3 without cooling system. The experimental design consisted of a latin rectangle repeated twice. The environmental variables (dry bulb temperature), black globe temperature and relative humidity were collected daily through dataloggers throughout the experiment. In behavioral analysis were observed postures (standing and lying), the activities of the animals and the places where they were in the pen, in 15 minute intervals. However, there was no difference in the behavioral variables of animals between treatments. (AU)

Assuntos

RESUMO

A pesquisa teve como objetivo avaliar a influência do sistema de resfriamento adiabático evaporativo (SRAE) sobre as variáveis comportamentais de novilhas leiteiras da raça Holandesa mantidas em confinamento. Foram utilizadas 12 novilhas, distribuídas em três tratamentos: 1) ventilação e SRAE por nebulização quando a temperatura atingia 25°C a qualquer umidade; 2) ventilação e SRAE por nebulização quando a temperatura atingia 25°C com umidade relativa menor ou igual a 70% e, 3 sem sistema de resfriamento. O delineamento experimental consistiu em um retângulo latino repetido duas vezes. As variáveis ambientais (temperatura do bulbo seco, temperatura do globo negro e umidade relativa foram coletadas diariamente, num intervalo de 15 minutos, através de dataloggers, durante todo o experimento. Na análise comportamental foram observadas as posturas (em pé e deitada), as atividades realizadas pelos animais e os locais em que se encontravam na baia, em intervalos de 15 minutos. Contudo, observou-se que não houve diferença nas variáveis comportamentais dos animais entre os tratamentos.

The research aimed to evaluate the influence of evaporative cooling system (AECS) on the behavioral variables of Holstein dairy heifers kept in confinement. Twelve heifers were used, divided into three treatments: 1) ventilation and fogging by AECS when the temperature reached 25°C at any humidity, 2) ventilation and fogging by AECS when the temperature reached 25°C with relative humidity less than or equal to 70% and, 3 without cooling system. The experimental design consisted of a latin rectangle repeated twice. The environmental variables (dry bulb temperature), black globe temperature and relative humidity were collected daily through dataloggers throughout the experiment. In behavioral analysis were observed postures (standing and lying), the activities of the animals and the places where they were in the pen, in 15 minute intervals. However, there was no difference in the behavioral variables of animals between treatments.

Assuntos