RESUMO
Graphene compounds are incorporated into polymers in order to improve their mechanical properties and in dentistry this modification favors the clinical performance of these materials. The aim of this review was to evaluate graphene compounds, their concentrations, and their effect on mechanical properties as flexural, tensile, and compressive strength and hardness of polymethylmethacrylate (PMMA) and polyether-ether-ketone (PEEK) for dental application. The search was carried out in two steps in PubMed/Medline, Embase, Scopus, and Web of Science databases. The eligibility criteria included studies that incorporated pure graphene compounds into dental polymers and evaluated their mechanical properties. Were found 4984 results, of which 11 articles were included in this review. Graphene compounds: graphene oxide (GO), reduced graphene oxide (rGO), and graphene nanoplatelets (GNP) were incorporated into PMMA and PEEK, in concentrations ranging from 0.1 to 10 wt%. Concentrations lower than 0.75 wt% of GO in PMMA and 1 wt% of GNP in PEEK resulted in increased flexural, tensile, compression strength, and hardness of these polymers. It was concluded that the incorporation of graphene compounds in low concentrations increases dental polymers' mechanical properties.
RESUMO
BACKGROUND: Inhibition of Candida albicans on denture resins could play a significant role in preventing the development of denture stomatitis. The safety of a new dental material with antifungal properties was analyzed in this work. METHODS: Poly(methyl methacrylate) [PMMA] discs and PMMA-silver nanoparticle discs were formulated, with the commercial acrylic resin, Nature-Cryl™, used as a control. Silver nanoparticles were synthesized and characterized by ultraviolet-visible spectroscopy, dispersive Raman spectroscopy, and transmission electron microscopy. The antifungal effect was assessed using a luminescent microbial cell viability assay. Biocompatibility tests were carried out using NIH-3T3 mouse embryonic fibroblasts and a Jurkat human lymphocyte cell line. Cells were cultured for 24 or 72 hours in the presence or absence of the polymer formulations and analyzed using three different tests, ie, cellular viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation by enzyme-linked immunosorbent assay BrdU, and genomic DNA damage (Comet assay). Finally, the samples were evaluated mechanically, and the polymer-bearing silver nanoparticles were analyzed microscopically to evaluate dispersion of the nanoparticles. RESULTS: The results show that PMMA-silver nanoparticle discs significantly reduce adherence of C. albicans and do not affect metabolism or proliferation. They also appear not to cause genotoxic damage to cells. CONCLUSION: The present work has developed a new biocompatible antifungal PMMA denture base material.
Assuntos
Resinas Acrílicas/síntese química , Candida albicans/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Materiais Dentários/síntese química , Dentaduras/microbiologia , Nanopartículas Metálicas/administração & dosagem , Prata/farmacologia , Animais , Antifúngicos/farmacologia , Materiais Biocompatíveis/síntese química , Materiais Biocompatíveis/farmacologia , Candida albicans/fisiologia , Adesão Celular/efeitos dos fármacos , Humanos , Células Jurkat , Teste de Materiais , Nanopartículas Metálicas/química , Camundongos , Células NIH 3T3 , Polimetil Metacrilato , Prata/químicaRESUMO
Purpose: To evaluate the cumulative effect of two protocols of microwave disinfection (Protocol 1: 690 W/6 min; Protocol 2: 345 W/6 min) on surface roughness (Ra) and baseplate adaptation of two denture resins polymerized by microwave energy (Onda Cryl) or hot water bath(QC-20). Methods: For Ra evaluation, rectangular specimens were fabricated and subjected to the following disinfection treatment (n=8/group): 1) Control (no disinfection), 2) Protocol 1, and 3) Protocol 2. Ra was measured using a profilometer at baseline (T0) and after two disinfection procedures (T1, T2) with a 7-day interval immersed in distilled water. To evaluate dimensional stability, maxillary baseplates were divided into the disinfection treatment groups (n=6), and adaptation was measured by weighing a silicon film reproducing the gap between resin baseplate and a metallic master model. Results: Ra had a significant interaction between type of resin/polymerization technique, disinfection treatment, and number of disinfection procedures (P<0.001). For Ra, Protocol 2 and control groups had similar increase of Ra over time, but Protocol 1 groups showed a significant decrease of Ra in T2. Baseplate adaptation was affected by a significant interaction between disinfection treatment and number of disinfection procedures (P<0.001), and Protocol 1 yielded the largest mean in T2. Conclusion: Onda Cryl displayed greater changes of Ra and adaptation than QC-20. Protocol 1 promoted overall damage to both types of denture resins after two disinfection procedures.
Objetivo: Avaliar o efeito cumulativo de dois protocolos de desinfecção por energia de micro-ondas (Protocolo 1: 690 W/6 min; Protocolo 2: 345 W/6 min) na rugosidade superficial (Ra) e adaptação de bases de dentadura de duas resinas polimerizadas por micro-ondas (Onda Cryl) ou banho de água quente (QC-20). Metodologia: Para Ra, espécimes retangulares foram fabricados e dividid os de acordo com o tratamento de desinfecção (n=8/grupo): 1) Controle (sem desinfecção), 2) Protocolo 1, e3) Protocolo 2. Para medir Ra usou-se um rugosímetro no baseline (T0) e após 2 procedimentos de desinfecção (T1, T2) com intervalo de 7 dias de imersão em água destilada. Para avaliar a estabilidade dimensional, bases maxilares foram divididas nos 3 grupos (n=6) e a adaptação foi medida por pesagem de uma película de silicone que reproduzia o espaço entre a base de resina e um modelo-mestre metálico. Resultados: Para Ra, houve interação significativa entre tipo de resina e técnica de polimerização, tratamento de desinfecção, e número de procedimentos desinfecção (P<0,001). Os grupos Protocolo 2 e controle apresentaram aumento semelhante de Ra ao longo do tempo, mas os grupos Protocolo 1 tiveram uma redução significativa de Ra em T2. A adaptação da base foi afetada por uma interação significante entre tratamento de desinfecção e número de procedimentos de desinfecção (P<0,001); o Protocolo 1 apresentou a maior média em T2. Conclusão: Onda Cryl teve maiores alterações de Ra e adaptação que QC-20. O Protocolo 1 promoveu dano geral para ambos os tipos de resina após dois procedimentos de desinfecção.