RESUMO
The "oviposition preference-offspring performance" hypothesis proposes that females lay their eggs in habitats that maximize the fitness of their offspring. The aim of this study was to assess the oviposition site selection by Aedes aegypti females and the success of their larvae in habitats with different detritus accumulation times, under conditions representative of the natural spatial variability of detritus quality and quantity in the Metropolitan Area of Buenos Aires, Argentina. Two experiments were performed, one assessing oviposition site selection and the other analyzing developmental success. In both experiments, two levels of detritus accumulation time were compared, one with short-time detritus accumulation (2 weeks), and the other with long-time detritus accumulation (8 weeks). Naturally fallen detritus was used in both experiments, collected in ten sites across the Metropolitan Area of Buenos Aires. In the oviposition experiment, two contiguous ovitraps corresponding to each accumulation time were placed at each of the ten sites and the number of eggs received for each accumulation time was compared. In the development experiment, always 19 larvae were raised in containers of both accumulation times and overall performance was compared using an integrated index that considers survival, development time and female size. A large variability in the amount of detritus collected at the different sites was observed. The number of eggs was significantly higher in the long-time than in the short-time detritus accumulation containers, and approximately proportional to the amount of detritus in each ovitrap. The performance was not affected by the detritus accumulation time, but a better performance was detected in containers that received a higher amount of organic detritus, regardless of the accumulation time. Leaves were on average the most abundant type of detritus, with an average of 53 % of the total detritus collected. The amount of leaves added 2 weeks before hatching showed a positive effect on larval performance. Our results do not support the "oviposition preference-offspring performance" hypothesis, since Ae. aegypti females laid eggs in containers where larvae did not show a better performance. Moreover, at larval densities related to the number of eggs actually laid in each of the accumulation times, it is expected that the performance would be even worse in the most selected containers, due to the density-dependent effects of crowding. Since the results obtained reflect the natural heterogeneity of the environmental conditions in the region studied, they might be a fairly good indicator of what occurs in natural larval habitats.
Assuntos
Aedes , Larva , Oviposição , Animais , Aedes/fisiologia , Aedes/crescimento & desenvolvimento , Feminino , Argentina , Larva/fisiologia , Larva/crescimento & desenvolvimento , Ecossistema , Fatores de TempoRESUMO
We aimed to determine how the degree of urbanization in a Neotropical city influences Aedes aegypti (L.), a pantropical vector of urban yellow fever, dengue, Zika and Chikungunia, via other mosquito species, whether they are competitors or predators, native to the area or invasive. We conducted experiments twice a month during one year in the city of Kourou, French Guiana, on three sites characterized by increasing percentages of imperviousness (i.e., 0.65%, 33.80% and 86.60%). These sites were located in a ≈5 ha forest fragment, a residential area with gardens, and in the older part of the city, respectively, and correspond to slightly, moderately and highly urbanized sites. There, we monitored twice a month during one year a total of 108 mosquito communities inhabiting four types of containers (i.e., a tank bromeliad, dry stumps of bamboo, ovitraps and car tires) installed in a random block design. In the tanks of the bromeliad, likely due to the acidity of the water, the immatures of native mosquito species prevailed, particularly Wyeomyia pertinans (Williston) in the slightly urbanized site. The general pattern was very similar in the three other types of containers where Limatus durhamii Théobald dominated in the slightly urbanized site, so that the abundance of Ae. aegypti immatures was low compared to those of native species. Yet, Ae. aegypti strongly dominated in the two more urbanized sites. These findings open up perspectives for vector management, including the conservation and/or the augmentation of natural enemies through modifications to landscape features.
Assuntos
Aedes , Infecção por Zika virus , Zika virus , Animais , Urbanização , Água , Mosquitos Vetores , CidadesRESUMO
The application of nuclear technologies in a cargo and container inspection facility can increase the risk of accidents. Estimating the radiation dose in the controlled area generates critical information for elaborating routines aimed at establishing more effective safety procedures. For radiological protection purposes, mapping ambient dose equivalent H*(10) levels is crucial. The radiation source used was a fixed linear accelerator of 4.5 MeV. Five RadEye PRD-ER (Thermo Fisher Scientific) personal radiation monitors and five Geiger-Müller MRAD 111 (Ultra Radac) personal radiation monitors were used for the radiation measurements. The highest ambient equivalent dose rate and dose per scan were found with the Geiger-Müller monitors at values of 5.76E-01 mSv/h and 1.12E-03 mSv, respectively. The results showed that for public individuals, the number of scans at the point of highest dose rate value cannot exceed 893-unit operations. Additionally, the risks involved in the abnormal situation (increased H*(10)) were estimated by using a model to predict the development of solid cancer as a result of occupational radiological exposure. This procedure highlights the risks involved, hence providing initial support to the decision process.
Assuntos
Exposição Ocupacional , Monitoramento de Radiação , Proteção Radiológica , Humanos , Exposição Ocupacional/análise , Doses de Radiação , Monitoramento de Radiação/métodos , Proteção Radiológica/métodosRESUMO
Native plants such asMelochia pyramidatahave been targeted in various scientific studies due to their potential uses. From this perspective, the present study aimed to separately evaluate the effect of different substrates and pot sizeson the production of seedlings of M. pyramidatagenotypes propagated by cuttings. Theexperimentwas conducted in a plant nursery at the Center of Agricultural Sciences of theFederal University of Paraíba, Areia, PB, Brazil. Theexperimental design was completely randomizedandarranged in a 3 x 2factorialdesignwith three substrate types (sand, commercial substrate Plantmax®, andsand+ manure) and two genotypes(MP1 andMP2), withfive replications. Pot size evaluation used a 2 x 2factorial designwith two pot sizes(V1: 1.8 LandV2: 0.9 L), two genotypes(MP1 andMP2), andfour replications. The evaluations began seven days after the cuttings were planted in tubes and continued weekly for two months by evaluating the number of sprouts and leaves. After two months, these seedlings were transplanted to pots and evaluated for the number of sprouts, leaves, fruits, flower buds, leaf width, and leaf length. The data were subjected to analysis of variance and test of means(Tukey) at5% probability. The cuttings ofM. pyramidatadeveloped best in washed sand and in the commercial substratePlantmax®. The genotypes developed best in 1.8-L pots, showing more leaves, flower buds, and fruits.
As plantas nativas, como a Melochia pyramidata, têm sido alvos de vários estudos científicos devido suas potencialidades de usos. O objetivo deste trabalho foi avaliar, separadamente, o efeito de substratos e tamanhos de vasos na produção de mudas de genótipos de M. pyramidata pelo método da estaquia. O experimento foi conduzido em casa de vegetação no Centro de Ciências Agrárias da Universidade Federal da Paraíba, Areia, PB, Brasil. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 3 x 2, com três tipos de substratos (areia, substrato comercial Plantmax® e areia + esterco) e 2 genótipos (MP1 e MP2.), com 5 repetições. Para a avaliação feita em diferentes tamanhos de vasos, utilizou-se o esquema fatorial 2 x 2, com 2 tamanhos de vasos (V1: 1,8 Litros e V2: 0,9 Litros) e 2 genótipos (MP1 eMP2), com 4 repetições. As avaliações iniciaram-se após sete dias do plantio das estacas nos tubetes, e continuaram, semanalmente, por dois meses, avaliando o número de brotações e o número de folhas. Após os dois meses, estas mudas foram transplantadas para vasos, onde avaliou número de brotações, número de folhas, número de frutos, número de botões florais, largura da folha e comprimento da folha. Os dados foram submetidos à análise de variância e teste de médias (Tukey) a 5% de probabilidade. As estacasde M. pyramidata apresentaram melhor desenvolvimento em areia lavada e substrato comercial Plantmax®. Os genótipos se desenvolveram melhor em vasos de 1,8 litros, com maiores quantidades de folhas, botões florais e frutos.
Assuntos
Malvaceae/crescimento & desenvolvimento , Substratos para Tratamento Biológico/análiseRESUMO
Native plants such asMelochia pyramidatahave been targeted in various scientific studies due to their potential uses. From this perspective, the present study aimed to separately evaluate the effect of different substrates and pot sizeson the production of seedlings of M. pyramidatagenotypes propagated by cuttings. Theexperimentwas conducted in a plant nursery at the Center of Agricultural Sciences of theFederal University of Paraíba, Areia, PB, Brazil. Theexperimental design was completely randomizedandarranged in a 3 x 2factorialdesignwith three substrate types (sand, commercial substrate Plantmax®, andsand+ manure) and two genotypes(MP1 andMP2), withfive replications. Pot size evaluation used a 2 x 2factorial designwith two pot sizes(V1: 1.8 LandV2: 0.9 L), two genotypes(MP1 andMP2), andfour replications. The evaluations began seven days after the cuttings were planted in tubes and continued weekly for two months by evaluating the number of sprouts and leaves. After two months, these seedlings were transplanted to pots and evaluated for the number of sprouts, leaves, fruits, flower buds, leaf width, and leaf length. The data were subjected to analysis of variance and test of means(Tukey) at5% probability. The cuttings ofM. pyramidatadeveloped best in washed sand and in the commercial substratePlantmax®. The genotypes developed best in 1.8-L pots, showing more leaves, flower buds, and fruits.(AU)
As plantas nativas, como a Melochia pyramidata, têm sido alvos de vários estudos científicos devido suas potencialidades de usos. O objetivo deste trabalho foi avaliar, separadamente, o efeito de substratos e tamanhos de vasos na produção de mudas de genótipos de M. pyramidata pelo método da estaquia. O experimento foi conduzido em casa de vegetação no Centro de Ciências Agrárias da Universidade Federal da Paraíba, Areia, PB, Brasil. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 3 x 2, com três tipos de substratos (areia, substrato comercial Plantmax® e areia + esterco) e 2 genótipos (MP1 e MP2.), com 5 repetições. Para a avaliação feita em diferentes tamanhos de vasos, utilizou-se o esquema fatorial 2 x 2, com 2 tamanhos de vasos (V1: 1,8 Litros e V2: 0,9 Litros) e 2 genótipos (MP1 eMP2), com 4 repetições. As avaliações iniciaram-se após sete dias do plantio das estacas nos tubetes, e continuaram, semanalmente, por dois meses, avaliando o número de brotações e o número de folhas. Após os dois meses, estas mudas foram transplantadas para vasos, onde avaliou número de brotações, número de folhas, número de frutos, número de botões florais, largura da folha e comprimento da folha. Os dados foram submetidos à análise de variância e teste de médias (Tukey) a 5% de probabilidade. As estacasde M. pyramidata apresentaram melhor desenvolvimento em areia lavada e substrato comercial Plantmax®. Os genótipos se desenvolveram melhor em vasos de 1,8 litros, com maiores quantidades de folhas, botões florais e frutos.(AU)
Assuntos
Malvaceae/crescimento & desenvolvimento , Substratos para Tratamento Biológico/análiseRESUMO
Tap water quality concerns and advertisements often drive increased bottled water consumption, especially in communities with historical tap water quality problems (e.g., Nogales, Arizona). The study objective was to assess contamination of municipal tap and bottled water in Nogales, Arizona. Bottled (sealed, open/partially consumed bottles, and reusable containers for vended water) and tap water samples were collected from 30 homes and analyzed for chemical and microbial contaminants. Fisher exact tests and Wilcoxon rank sum tests were used to compare proportions of positive samples and contaminant concentrations between tap and bottled water samples. While none of the chemical contaminants were above MCLs, there were statistically significantly greater concentrations and proportions of positive samples for some contaminants, including arsenic, in tap vs. bottled water. E. coli concentrations were >0 CFU/100mL in some unsealed bottled water samples but not for sealed bottles. This study demonstrates that 1) the measured concentrations in tap and bottled water likely pose low risks, as they are below the MCLs, 2) more education in this community on hygiene maintenance of refillable or opened bottled water containers is needed, and 3) using tap water over bottled water is advantageous due to likely lower E. coli risk and lower cost.
RESUMO
The data produced by sensors of IoT devices are becoming keystones for organizations to conduct critical decision-making processes. However, delivering information to these processes in real-time represents two challenges for the organizations: the first one is achieving a constant dataflow from IoT to the cloud and the second one is enabling decision-making processes to retrieve data from dataflows in real-time. This paper presents a cloud-based Web of Things method for creating digital twins of IoT devices (named sentinels).The novelty of the proposed approach is that sentinels create an abstract window for decision-making processes to: (a) find data (e.g., properties, events, and data from sensors of IoT devices) or (b) invoke functions (e.g., actions and tasks) from physical devices (PD), as well as from virtual devices (VD). In this approach, the applications and services of decision-making processes deal with sentinels instead of managing complex details associated with the PDs, VDs, and cloud computing infrastructures. A prototype based on the proposed method was implemented to conduct a case study based on a blockchain system for verifying contract violation in sensors used in product transportation logistics. The evaluation showed the effectiveness of sentinels enabling organizations to attain data from IoT sensors and the dataflows used by decision-making processes to convert these data into useful information.
RESUMO
Background: The management of empty pesticide containers (EPC) in Bolivia has been recently promoted as a control strategy for dispersed pollution in surface and underground water bodies, as well as in soil. It comprises the rinsing and proper disposal or reuse of clean EPC. However, the rinsing transfers the hazard to water, which must be properly treated before being discharged. Methods: In this study, solar photo-Fenton at low Fe 2+ doses were tested at pilot plant scale in Cochabamba (Bolivia) for the removal of pesticides dimethoate and atrazine in their commercial form, spiked in river water. Results: The results demonstrated that solar photo-Fenton ([H 2O 2] 0 = 200 mg L -1) with Fe 2+ concentrations between 0.5 and 1.5 mg L -1 is an effective method to remove dimethoate and atrazine, at an initial concentration of 10 mg L -1 each. Efficiency increased when increasing Fe 2+ doses, achieving a removal over 99% of both pesticides after a solar irradiation period of 60 minutes (corresponding to an accumulated energy of 4.96 kJ L -1). Conclusions: The presence of high concentrations of natural components of river water, mainly organic and inorganic carbon species, would have contributed to hydroxyl radical scavenging, explaining, together with the low iron dose applied, the high energy (irradiation time) and high hydrogen peroxide concentration required to produce pesticide depletion. Additionally, the relatively low oxidant consumption and mineralization observed leave room for process optimization regarding oxidant and catalyst doses and warrant further studies on its coupling with biological or other post-treatments for the removal of transformation products.
RESUMO
Native plants such asMelochia pyramidatahave been targeted in various scientific studies due to their potential uses. From this perspective, the present study aimed to separately evaluate the effect of different substrates and pot sizeson the production of seedlings of M. pyramidatagenotypes propagated by cuttings. Theexperimentwas conducted in a plant nursery at the Center of Agricultural Sciences of theFederal University of Paraíba, Areia, PB, Brazil. Theexperimental design was completely randomizedandarranged in a 3 x 2factorialdesignwith three substrate types (sand, commercial substrate Plantmax®, andsand+ manure) and two genotypes(MP1 andMP2), withfive replications. Pot size evaluation used a 2 x 2factorial designwith two pot sizes(V1: 1.8 LandV2: 0.9 L), two genotypes(MP1 andMP2), andfour replications. The evaluations began seven days after the cuttings were planted in tubes and continued weekly for two months by evaluating the number of sprouts and leaves. After two months, these seedlings were transplanted to pots and evaluated for the number of sprouts, leaves, fruits, flower buds, leaf width, and leaf length. The data were subjected to analysis of variance and test of means(Tukey) at5% probability. The cuttings ofM. pyramidatadeveloped best in washed sand and in the commercial substratePlantmax®. The genotypes developed best in 1.8-L pots, showing more leaves, flower buds, and fruits.(AU)
As plantas nativas, como a Melochia pyramidata, têm sido alvos de vários estudos científicos devido suas potencialidades de usos. O objetivo deste trabalho foi avaliar, separadamente, o efeito de substratos e tamanhos de vasos na produção de mudas de genótipos de M. pyramidata pelo método da estaquia. O experimento foi conduzido em casa de vegetação no Centro de Ciências Agrárias da Universidade Federal da Paraíba, Areia, PB, Brasil. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 3 x 2, com três tipos de substratos (areia, substrato comercial Plantmax® e areia + esterco) e 2 genótipos (MP1 e MP2.), com 5 repetições. Para a avaliação feita em diferentes tamanhos de vasos, utilizou-se o esquema fatorial 2 x 2, com 2 tamanhos de vasos (V1: 1,8 Litros e V2: 0,9 Litros) e 2 genótipos (MP1 eMP2), com 4 repetições. As avaliações iniciaram-se após sete dias do plantio das estacas nos tubetes, e continuaram, semanalmente, por dois meses, avaliando o número de brotações e o número de folhas. Após os dois meses, estas mudas foram transplantadas para vasos, onde avaliou número de brotações, número de folhas, número de frutos, número de botões florais, largura da folha e comprimento da folha. Os dados foram submetidos à análise de variância e teste de médias (Tukey) a 5% de probabilidade. As estacasde M. pyramidata apresentaram melhor desenvolvimento em areia lavada e substrato comercial Plantmax®. Os genótipos se desenvolveram melhor em vasos de 1,8 litros, com maiores quantidades de folhas, botões florais e frutos.(AU)
Assuntos
Substratos para Tratamento Biológico/métodos , Malvaceae/crescimento & desenvolvimento , Malvaceae/genéticaRESUMO
This study investigated spatial and temporal variations on the coexistence of Aedes aegypti, Aedes albopictus, and Culex larvae in five subdistricts in Nakhon Si Thammarat, Thailand. We tested two main hypotheses on the spatial and temporal coexistence of mosquito larvae: (1) condition-specific competition and (2) spatial variation among the five subdistricts. We compared the number of positive houses, positive containers, mosquito coexistence during both the wet and dry seasons. The results showed that from a total of 1,072 positive containers collected in both seasons, Ae. albopictus larvae were found in the highest number of containers (745 containers), followed by containers with Ae. aegypti larvae (283 containers) and containers with Culex larvae (254 containers). During the wet season, there were higher numbers of positive houses, positive containers, and containers with only Ae. albopictus larvae than during the dry season. On the other hand, during the dry season when the water containers were very scarce, there were higher numbers of containers with Culex larvae, and containers held more than one type of mosquito larvae. This indicates that both temporal and spatial variations may contribute to the local coexistence of Aedes and Culex mosquito larvae species in Lansaka District areas in Thailand.
Assuntos
Animais , Aedes/crescimento & desenvolvimento , Culex/crescimento & desenvolvimento , Desidratação/prevenção & controle , Estação ChuvosaRESUMO
This study investigated spatial and temporal variations on the coexistence of Aedes aegypti, Aedes albopictus, and Culex larvae in five subdistricts in Nakhon Si Thammarat, Thailand. We tested two main hypotheses on the spatial and temporal coexistence of mosquito larvae: (1) condition-specific competition and (2) spatial variation among the five subdistricts. We compared the number of positive houses, positive containers, mosquito coexistence during both the wet and dry seasons. The results showed that from a total of 1,072 positive containers collected in both seasons, Ae. albopictus larvae were found in the highest number of containers (745 containers), followed by containers with Ae. aegypti larvae (283 containers) and containers with Culex larvae (254 containers). During the wet season, there were higher numbers of positive houses, positive containers, and containers with only Ae. albopictus larvae than during the dry season. On the other hand, during the dry season when the water containers were very scarce, there were higher numbers of containers with Culex larvae, and containers held more than one type of mosquito larvae. This indicates that both temporal and spatial variations may contribute to the local coexistence of Aedes and Culex mosquito larvae species in Lansaka District areas in Thailand.(AU)
Assuntos
Animais , Aedes/crescimento & desenvolvimento , Culex/crescimento & desenvolvimento , Desidratação/prevenção & controle , Estação ChuvosaRESUMO
BACKGROUND: In El Salvador, Aedes aegypti mosquitoes transmitting Zika and other arboviruses use water storage containers as important oviposition sites. Promotion of water storage container cleaning is a key element of prevention programs. We explored community perceptions surrounding cleaning practices among pregnant women, male partners of pregnant women, and women likely to become pregnant. METHODS: Researchers conducted 11 focus groups and 12 in-depth interviews which included individual elicitations of Zika prevention measures practiced in the community. Focus group participants rated 18 images depicting Zika-related behaviors according to effectiveness and feasibility in the community context, discussed influencing determinants, voted on community intentions to perform prevention behaviors, and performed washbasin cleaning simulations. In-depth interviews with male partners of pregnant women used projective techniques with images to explore their perceptions on a subset of Zika prevention behaviors. RESULTS: General cleaning of the home, to ensure a healthy environment, was a strong community norm. In this context, participants gave water storage container cleaning a high rating, for both its effectiveness and feasibility. Participants were convinced that they cleaned their water storage containers effectively against Zika, but their actual skills were inadequate to destroy Aedes aegypti eggs. A further constraint was the schedule of water availability. Even during pregnancy, male partners rarely cleaned water storage containers because water became available in homes when they were at work. Furthermore, prevailing gender norms did not foster male participation in domestic cleaning activities. Despite these factors, many men were willing to provide substantial support with cleaning when their partners were pregnant, in order to protect their family. CONCLUSIONS: Behavior change programs for the prevention of Zika and other arboviruses need to improve community members' mosquito egg destruction skills rather than perpetuate the promotion of non-specific cleaning in and around the home as effective. Egg elimination must be clearly identified as the objective of water storage container maintenance and programs should highlight the effective techniques to achieve this goal. In addition, programs must build the skills of family members who support pregnant women to maintain the frequency of effective egg destruction in all water storage containers of the home.
Assuntos
Aedes/virologia , Conhecimentos, Atitudes e Prática em Saúde , Controle de Mosquitos/métodos , Abastecimento de Água , Água , Infecção por Zika virus/prevenção & controle , Zika virus , Adolescente , Adulto , Animais , El Salvador , Feminino , Grupos Focais , Humanos , Masculino , Pessoa de Meia-Idade , Gravidez , Pesquisa Qualitativa , Características de Residência , Adulto Jovem , Zika virus/crescimento & desenvolvimento , Infecção por Zika virus/transmissão , Infecção por Zika virus/virologiaRESUMO
BACKGROUND: As no globally accepted dengue vaccines or specific antiviral therapies are currently available, controlling breeding sites of Aedes aegypti is a target to prevent dengue outbreaks. The present study aimed to characterize outdoor artificial breeding sites in urban households using an exhaustive classification system. METHODS: A cross-sectional entomological survey was carried out in Colón city, Entre Ríos, Argentina, using a two-stage stratified sampling design during March and April 2014. The city was stratified given the degree of urbanization of each block, and blocks and households were randomly selected. All outdoor containers with water were inspected, and the presence of immature mosquitoes was recorded. Containers were classified according to physical, functional, and location attributes. Generalized linear mixed models were applied to take into account the aggregated nature of the data (containers in houses and houses in blocks). RESULTS: Overall, 207 houses were inspected. Out of 522 containers with water, 25% had immatures of Ae. aegypti (7336). In adjusted models, the abundance of immatures was higher in containers with increasing opening surface and volume, without roof cover, exposed to shadow, out of use or with functions related to gardening activities, household chores, water storage, or construction. At block level, immatures abundance was positively associated with the degree of urbanization. CONCLUSIONS: We detected high immatures abundance in containers associated with water utilization. This suggests that containers involved in these activities, whether directly (e.g., water storage) or indirectly (e.g., incomplete water drainage in the last use), are susceptible to present a high immature abundance. Although our results indicate the importance of the type of use over the type of container, we encourage the use of both classification criteria for artificial breeding sites of mosquitoes, mainly because these are complementary. Additionally, generalized linear mixed models allowed us to analyse predictor variables at different scales (container/house/block) and consider the lack of independence between observations. An exhaustive analysis of artificial breeding sites that use this analytical methodology can lead to new information that could help designing more appropriate tools for dengue surveillance and control.
Assuntos
Aedes/fisiologia , Cruzamento , Microbiologia da Água , Animais , Argentina , Estudos Transversais , Dengue/parasitologia , Ecossistema , Habitação , Humanos , Modelos Lineares , População UrbanaRESUMO
Usually, Culex mosquitoes are responsible for spreading several human diseases such as malaria, Japanese encephalitis, yellow fever, filariasis, and Zika. Lymphatic filariasis and Zika cases are increasing in Thailand with more prevalent in southern Thailand. In this study, our aim is to examine how seasons and water container types affect Culex spp. larvae numbers in Lansaka district, Nakhon Si Thammarat province, southern Thailand. This study was conducted in five sub-districts (Lansaka, Khaokaew, Thadi, Kamlon, and Khunthale) in Lansaka district. In each subdistrict, 120 houses were randomly selected to study the breeding sites of Culex mosquito in dry (March-May, 2018) and wet (October-December, 2018) seasons. Mosquito larvae were collected from indoor, outdoor, natural, artificial, darkcoloured, light-coloured, with lid, and without lid containers. It was observed that in all sub-districts, Culex larvae numbers were higher in dry season compared to wet season. In both seasons, outdoor, without lid, dark-coloured, and artificial containers contained more Culex larvae than inside, with lid, light-coloured, and natural containers, respectively. This study shows that seasons and water container types affect Culex mosquito larvae. Since the larvae numbers are higher in dry season, as well as in outdoor, without lid, dark-coloured, and artificial containers, people should reduce the usage of these containers, especially in the dry season, to prevent Culex borne diseases.
Assuntos
Culex/crescimento & desenvolvimento , Larva , Tailândia/epidemiologia , Estação Chuvosa , Estação SecaRESUMO
Usually, Culex mosquitoes are responsible for spreading several human diseases such as malaria, Japanese encephalitis, yellow fever, filariasis, and Zika. Lymphatic filariasis and Zika cases are increasing in Thailand with more prevalent in southern Thailand. In this study, our aim is to examine how seasons and water container types affect Culex spp. larvae numbers in Lansaka district, Nakhon Si Thammarat province, southern Thailand. This study was conducted in five sub-districts (Lansaka, Khaokaew, Thadi, Kamlon, and Khunthale) in Lansaka district. In each subdistrict, 120 houses were randomly selected to study the breeding sites of Culex mosquito in dry (March-May, 2018) and wet (October-December, 2018) seasons. Mosquito larvae were collected from indoor, outdoor, natural, artificial, darkcoloured, light-coloured, with lid, and without lid containers. It was observed that in all sub-districts, Culex larvae numbers were higher in dry season compared to wet season. In both seasons, outdoor, without lid, dark-coloured, and artificial containers contained more Culex larvae than inside, with lid, light-coloured, and natural containers, respectively. This study shows that seasons and water container types affect Culex mosquito larvae. Since the larvae numbers are higher in dry season, as well as in outdoor, without lid, dark-coloured, and artificial containers, people should reduce the usage of these containers, especially in the dry season, to prevent Culex borne diseases.(AU)
Assuntos
Culex/crescimento & desenvolvimento , Larva , Tailândia/epidemiologia , Estação Seca , Estação ChuvosaRESUMO
Abstract Mosquitoes (Diptera: Culicidae) are important vectors of diverse pathogenic organisms, particularly arboviruses and protozoa. The immature stages of mosquitoes occur in a wide range of natural or human-made aquatic habitats. On this basis, some anthropogenic places such as cemeteries, usually serve as thrive and productive artificial-breeding habitats for mosquitoes. Despite being important foci for vector species, urban cemeteries are frequently overlooked in control and surveillance programs. This study evaluates the association of ecological variables and attributes (type of material, presence of flowers, water availability and height from the ground) of the breeding sites with the levels of infestation of mosquito immature stages. In 2017, an entire urban cemetery in Jarabacoa (Dominican Republic) was sampled at two different climatic periods (March: dry and August: rainy) for the characterization of the artificial breeding-sites, collection of immature stages of culicids and subsequent laboratory rearing for species identification. In total 968 containers were studied, containing 7 758 immature stages in 203 (21.0 %) water-filled containers which accounted for four species: Culex quinquefasciatus (50.5 %), Aedes aegypti (47.1 %), Aedes albopictus (1.9 %) and Culex nigripalpus (0.4 %). The mean of A. aegypti immatures in infested containers was roughly two times higher compared to C. quinquefasciatus and significantly lower compared to A. albopictus. The total Container Index (CI) was 20.9 %, and among the type of materials, those made from rock (cement, ceramic, and mud) and plastic had the highest CI = 25.9 % and 23.4 %, respectively. Almost 95 % of the total infested water-filled containers were made of plastic or rock. No association was found between the type of material of the containers and the density of mosquitoes. However, overall, greater densities of immature stages were found at ground than at higher levels. A weak positive correlation between water volume and density was found in some species of immature stages. Significantly higher number of C. quinquefasciatus were recorded in containers with flowers and large water volume. In contrast, A. aegypti immature stages were more frequent in containers without flowers. A weak negative association between water volume and infestations was found for A. albopictus immatures. As reflected of their opportunistic behaviour and broad ecological plasticity, Culex spp. and Aedes spp. mosquitoes were abundant pests in cemetery habitats where were able to breed in almost any kind of water-filled container regardless the type of material. However, our study showed that some ecological variables have critical impact for the development of the immature stages of some species. The health authorities and cemetery keepers can benefit from these results by focusing on the implementation of detailed plans and integrated strategies for the control and prevention of cemetery infestations by mosquitoes.(AU)
Resumen Los mosquitos (Diptera: Culicidae) son importantes vectores de diversos organismos patógenos, tales como arbovirus y protozoos. Los estadios inmaduros de los mosquitos pueden encontrarse en un amplio abanico de hábitats, tanto de origen natural como humano. Algunos lugares antrópicos, como los cementerios, sirven como prósperos hábitats artificiales para el desarrollo del ciclo de vida de los mosquitos. A pesar de constituir importantes focos para especies de interés vectorial, los cementerios urbanos son frecuentemente olvidados en los programas de vigilancia y control. Este estudio evaluó la asociación de algunas variables ecológicas (tipo de material, presencia de flores, disponibilidad de agua, y altura respecto al suelo) de los lugares de cría (recipientes) con los niveles de infestación de las fases preimarginales de los culícidos. En 2017, un cementerio urbano en Jarabacoa (República Dominicana) fue inspeccionado durante dos periodos climáticos (es decir: marzo: estación seca y agosto: estación húmeda) para: caracterizar los lugares de cría artificiales, captura de los estadios inmaduros de culícidos e identificación de los adultos emergidos a partir de las fases inmaduras. En total, 968 recipientes fueron examinados, conteniendo 7 758 inmaduros en 203 (21.0 %) recipientes con agua pertenecientes a cuatro especies, Culex quinquefasciatus (50.5 %), Aedes aegypti (47.1 %), Aedes albopictus (1.9 %) y Culex nigripalpus (0.4 %). El índice de recipiente (IR) fue del 20.9 %, y los recipientes fabricados a partir de roca (cemento, cerámica y barro) y plástico tuvieron el mayor IR = 25.9 y 23.4 %, respectivamente. Casi un 95 % de los recipientes con agua infectados estaban constituidos de materiales tipo plástico o roca. No se encontró asociación entre el tipo de material del recipiente y la densidad. En general, mayor densidad de estadios inmaduros fue observada a nivel del suelo que a alturas mayores. Se observó un incremento significativo del número de C. quinquefasciatus en recipientes con flores y grandes volúmenes de agua. Por el contrario, los estadios de A. aegypti fueron más frecuentes en recipientes sin flores. Como reflejo de su comportamiento oportunista y amplia plasticidad ecológica, los mosquitos Culex y Aedes fueron abundantes en los cementerios, siendo capaces de desarrollar su ciclo de vida acuático en cualquier tipo de recipiente, con independencia del material que lo constituya. Nuestro estudio, por tanto, mostró que algunas variables ecológicas tienen un impacto crítico sobre el desarrollo de los estadios inmaduros de algunas especies de mosquitos. Tanto las autoridades de salud pública como los cuidadores del cementerio pueden beneficiare de estos resultados centrándose en la implementación de planes detallados y estrategias integradoras para el control y prevención de mosquitos que infectan cementerios.(AU)
Assuntos
Aedes/patogenicidade , Culex/patogenicidade , Microbiologia Ambiental , Cemitérios , República DominicanaRESUMO
Las soluciones parenterales de gran volumen (SPGV) son formulaciones líquidas de administración intravenosa, en las cuales la esterilidad y apirogenicidad son requisitos indispensables. De manera semejante, los envases que las contienen deben cumplir estrictas especificaciones de manera de asegurar la integridad y la seguridad de estos productos durante toda su vida útil y, de este modo, disminuir la probabilidad de contaminación que pudiera poner en riesgo la salud de los pacientes. Por este motivo, para verificar el cumplimiento de la normativa vigente y evaluar la calidad, se realizó un estudio horizontal de mercado de productos de SPGV conteniendo metronidazol 500 mg/ 100 ml y ciprofloxacina 200 mg/100 ml en presentaciones de 100 ml. Los ensayos realizados fueron los establecidos por la Disposición ANMAT 1149/97. Los resultados demostraron que, de los cinco laboratorios incluidos en el estudio, uno debe modificar el material de sus envases a fin de evitar la permeabilidad al vapor de agua y los otros cuatro deben rever la capacidad de escurrimiento y colapsabilidad o incluir en su rótulo la leyenda indicada en el inciso E 3.1.4 de la citada norma, en cuanto a la obligación de ser administrados en forma aséptica con filtración del aire de ventilación. El presente trabajo resalta la importancia de las acciones de vigilancia activa llevada a cabo por la ANMAT para asegurar la eficacia, seguridad y calidad de los productos comercializados dentro de su ámbito regulatorio
Large Volume Parenterals (LVP's) are aqueous solutions intended for intravenous use. Sterility and being pyrogen-free are both essential requirements. Similarly, the containers of these solutions must meet strict specifications in order to ensure the integrity and safety of these products throughout their useful life and, thus, reduce the likelihood of contamination that could put at risk the patients' health. For this reason, to verify compliance with current regulations and assess quality, a horizontal market study of SPGV products containing metronidazole 500 mg / 100 ml and ciprofloxacin 200 mg / 100 ml in 100 ml presentations was conducted. The tests carried out were those established by Regulation ANMAT 1149/97. The results showed that, one of the five laboratories included in the study, must modify the material of the containers to avoid water vapor permeability and the other four must review the capacity of runoff and collapsibility or include in the label the legend indicated in the subsection. E 3.1.4 of the aforementioned standard, regarding the obligation to be aseptically administered with ventilation air filtration. This work highlights the importance of active surveillance actions carried out by ANMAT to ensure the effectiveness, safety and quality of products marketed within its regulatory scope
Assuntos
Ciprofloxacina , Embalagem de Medicamentos , Farmacovigilância , MetronidazolRESUMO
The objective of this study was to evaluate the morphophysiological quality of yerba mate plantlets (Ilex paraguariensis A. St.-Hil.) produced by mini-cutting at four different times of year and cultivated in different containers. For the rooting of mini-cuttings, shoots of four yerba mate clones were collected four times during the year (April, July and October 2014 and January, 2015), cut into single-budded mini-cuttings and planted in equal proportions of pine bark-based commercial substrate, vermiculite and coarse sand. After 60 days of cultivation in a wet chamber, the rooted mini-cuttings were transferred to different containers: a 100 cm³ rigid polyethylene tube container and 500, 1500 and 3000 cm3 polyethylene bags containing pine bark-based commercial substrate and underground soil (2:1 v/v). A completely randomized design was used in a 4 x 4 factorial scheme, with five replications ranging from 5 to 20 mini-cuttings per experimental unit. The plantlets were evaluated for survival percentage, shoot height, stem diameter, shoot height/stem diameter ratio and number of leaves at 30, 60, 90 and 120 days of cultivation. At 120 days of cultivation, 10 plantlets from each treatment were randomly picked to evaluate shoot and root dry mass, Dickson quality index, shoot height, surface area, total volume and number of root tips. The best combinations of shoot height, length, surface area, total volume and number of root tips and Dickson quality index were found in plantlets produced in July, 2014, followed by plantlets produced in October,2014 and January, 2015. The polyethylene bags allowed the production of yerba mate plantlets with satisfactory quality of shoot and roots, regardless of the volume. Yerba mate plantlets can be produced by mini-cutting from July to January in polyethylene bags.
O objetivo deste estudo foi avaliar a qualidade morfofisiológica de mudas de erva-mate (Ilex paraguariensis A. St.-Hil.) produzidas por miniestaquia em quatro épocas do ano e cultivadas em diferentes recipientes. Para o enraizamento das miniestacas, brotações de quatro clones de erva-mate foram coletadas em quatro épocas do ano (Abril, Julho e Outubro de 2014 e Janeiro de 2015), seccionadas em miniestacas de gema única e cultivadas em iguais proporções de substrato comercial à base de casca de pinus, vermiculita média e areia de granulometria grossa. Após 60 dias de cultivo em câmara úmida, as miniestacas enraizadas foram transferidas para diferentes recipientes: tubete de polietileno rígido de 100 cm³ e sacos de polietileno de 500, 1500 e 3000 cm3, contendo substrato comercial à base de casca de pinus e terra de subsolo (2:1 v/v). Foi utilizado um delineamento inteiramente casualizado em esquema fatorial 4 x 4, com cinco repetições variando de 5 a 20 miniestacas por unidade experimental. Aos 30, 60, 90 e 120 dias de cultivo, as mudas de erva-mate foram avaliadas quanto a porcentagem de sobrevivência, altura da parte aérea, diâmetro de colo, relação entre a altura da parte aérea e diâmetro de colo e número de folhas. Somente aos 120 dias de cultivo, 10 mudas de cada tratamento foram tomadas ao acaso para a avaliação da relação massa seca da parte aérea e das raízes, índice de qualidade de Dickson, comprimento total, área superficial, volume total e número de extremidades das raízes. Miniestacas de erva-mate coletadas em julho de 2014 resultaram em mudas com maior altura da parte aérea, comprimento, área superficial, volume total e número de extremidades das raízes e índice de qualidade de Dickson, seguidas daquelas coletadas em outubro de 2014 e janeiro de 2015. Os sacos de polietileno, independentemente do volume, permitiram a produção de mudas de erva-mate com qualidade satisfatória tanto da parte aérea quanto do sistema radicial.
Assuntos
Ilex paraguariensis/anatomia & histologia , Ilex paraguariensis/fisiologia , PolietilenoRESUMO
The objective of this study was to evaluate the morphophysiological quality of yerba mate plantlets (Ilex paraguariensis A. St.-Hil.) produced by mini-cutting at four different times of year and cultivated in different containers. For the rooting of mini-cuttings, shoots of four yerba mate clones were collected four times during the year (April, July and October 2014 and January, 2015), cut into single-budded mini-cuttings and planted in equal proportions of pine bark-based commercial substrate, vermiculite and coarse sand. After 60 days of cultivation in a wet chamber, the rooted mini-cuttings were transferred to different containers: a 100 cm³ rigid polyethylene tube container and 500, 1500 and 3000 cm3 polyethylene bags containing pine bark-based commercial substrate and underground soil (2:1 v/v). A completely randomized design was used in a 4 x 4 factorial scheme, with five replications ranging from 5 to 20 mini-cuttings per experimental unit. The plantlets were evaluated for survival percentage, shoot height, stem diameter, shoot height/stem diameter ratio and number of leaves at 30, 60, 90 and 120 days of cultivation. At 120 days of cultivation, 10 plantlets from each treatment were randomly picked to evaluate shoot and root dry mass, Dickson quality index, shoot height, surface area, total volume and number of root tips. The best combinations of shoot height, length, surface area, total volume and number of root tips and Dickson quality index were found in plantlets produced in July, 2014, followed by plantlets produced in October,2014 and January, 2015. The polyethylene bags allowed the production of yerba mate plantlets with satisfactory quality of shoot and roots, regardless of the volume. Yerba mate plantlets can be produced by mini-cutting from July to January in polyethylene bags.(AU)
O objetivo deste estudo foi avaliar a qualidade morfofisiológica de mudas de erva-mate (Ilex paraguariensis A. St.-Hil.) produzidas por miniestaquia em quatro épocas do ano e cultivadas em diferentes recipientes. Para o enraizamento das miniestacas, brotações de quatro clones de erva-mate foram coletadas em quatro épocas do ano (Abril, Julho e Outubro de 2014 e Janeiro de 2015), seccionadas em miniestacas de gema única e cultivadas em iguais proporções de substrato comercial à base de casca de pinus, vermiculita média e areia de granulometria grossa. Após 60 dias de cultivo em câmara úmida, as miniestacas enraizadas foram transferidas para diferentes recipientes: tubete de polietileno rígido de 100 cm³ e sacos de polietileno de 500, 1500 e 3000 cm3, contendo substrato comercial à base de casca de pinus e terra de subsolo (2:1 v/v). Foi utilizado um delineamento inteiramente casualizado em esquema fatorial 4 x 4, com cinco repetições variando de 5 a 20 miniestacas por unidade experimental. Aos 30, 60, 90 e 120 dias de cultivo, as mudas de erva-mate foram avaliadas quanto a porcentagem de sobrevivência, altura da parte aérea, diâmetro de colo, relação entre a altura da parte aérea e diâmetro de colo e número de folhas. Somente aos 120 dias de cultivo, 10 mudas de cada tratamento foram tomadas ao acaso para a avaliação da relação massa seca da parte aérea e das raízes, índice de qualidade de Dickson, comprimento total, área superficial, volume total e número de extremidades das raízes. Miniestacas de erva-mate coletadas em julho de 2014 resultaram em mudas com maior altura da parte aérea, comprimento, área superficial, volume total e número de extremidades das raízes e índice de qualidade de Dickson, seguidas daquelas coletadas em outubro de 2014 e janeiro de 2015. Os sacos de polietileno, independentemente do volume, permitiram a produção de mudas de erva-mate com qualidade satisfatória tanto da parte aérea quanto do sistema radicial.(AU)
Assuntos
Ilex paraguariensis/anatomia & histologia , Ilex paraguariensis/fisiologia , PolietilenoRESUMO
ABSTRACT We assessed the immature stages of Ceratopogonidae (Diptera) in artificial containers in an urban forest fragment in Manaus (Brazil), including their behavioral, biological and ecological information. In addition, we evaluated the effects of deforestation in an open and forested area on Ceratopogonidae communities. Immatures were sampled between August 2012 and July 2014 in artificial containers installed in both habitat types. We collected 685 immatures of seven morpho-species of Bezzia Kieffer, Culicoides Latreille, Dasyhelea Kieffer, Forcipomyia Meigen, and Palpomyia Meigen. In the open area, we recorded higher temperature and electrical conductivity values than in the forested area; however, these variables did not differ between seasons. Water volume was higher in open area and in rainy season, while pH was similar in both areas and seasons. Species richness was higher in forested area, but did not differ between seasons. We did not record differences in abundance between areas or seasons. Community composition differed between areas, but not between seasons. We provide the first records of Culicoides (Hoffmania) insignis Lutz and C. (Haematomyidium) quasiparaensis Clastrier in artificial containers from the state of Amazonas. Our results suggest that the preservation of forested areas in Amazonas is fundamental for the maintenance of the life cycle of some species of Ceratopogonidae.