RESUMO
Climate change has led to the search for strategies to acclimatize plants to various abiotic stressors to ensure the production and quality of crops of commercial interest. Sorghum is the fifth most important cereal crop, providing several uses including human food, animal feed, bioenergy, or industrial applications. The crop has an excellent adaptation potential to different types of abiotic stresses, such as drought, high salinity, and high temperatures. However, it is susceptible to low temperatures compared with other monocotyledonous species. Here, we have reviewed and discussed some of the research results and advances that focused on the physiological, metabolic, and molecular mechanisms that determine sorghum cold tolerance to improve our understanding of the nature of such trait. Questions and opportunities for a comprehensive approach to clarify sorghum cold tolerance or susceptibility are also discussed.
RESUMO
BACKGROUND: In subtropical areas, early planting exposes rice seedlings to cold stress, impairing seedling growth and making them more vulnerable to other stresses including herbicide injury. The objectives of this work were: to evaluate the effect of cold stress on bispyribac-sodium selectivity in rice; to determine the mechanisms of cold tolerance in sensitive ('Epagri 109') and tolerant ('IRGA 424') rice cultivars; and to ascertain that cold acclimatization influences bispyribac-sodium selectivity in rice. RESULTS: Prolonged cold stress caused high lipid peroxidation, increased rice injury, and stunted growth. Short-term acclimation with cold stress reduced rice injury with bispyribac-sodium. Total phenols were upregulated in rice exposed to cold stress. Prolonged cold stress increased the superoxide dismutase and catalase activity in IRGA 424. Antioxidant activity was higher in the cold-tolerant than in the cold-sensitive cultivar. Only catalase activity was responsive to bispyribac-sodium. OsRAN2, OsGSTL2, and CYP72A21 were upregulated by cold and herbicide stress in both cultivars. OsGSTL2 was upregulated more in IRGA 424 than in Epagri 109. OsFAD8 was upregulated in cold-sensitive rice exposed to short-duration cold stress but was not responsive to bispyribac-sodium. CONCLUSION: Cold stress reduces bispyribac-sodium selectivity in rice. Short-term acclimation to cold stress reduces the effect of cold stress and enhances bispyribac-sodium selectivity. The tolerance of rice (IRGA 424) to cold stress is due to differential induction of protection genes CYP72A21 and OsGSTL2 associated with herbicide metabolism, together with the accumulation of total phenols and higher activity of antioxidant enzymes.
Assuntos
Oryza , Aclimatação , Benzoatos , Temperatura Baixa , Resposta ao Choque Frio , Pirimidinas , Plântula/genética , TemperaturaRESUMO
Rice (Oryza sativa L.) ssp. indica is the most cultivated species in the South of Brazil. However, these plants face low temperature stress from September to November, which is the period of early sowing, affecting plant development during the initial stages of growth, and reducing rice productivity. This study aimed to characterize the root response to low temperature stress during the early vegetative stage of two rice genotypes contrasting in their cold tolerance (CT, cold-tolerant; and CS, cold-sensitive). Root dry weight and length, as well as the number of root hairs, were higher in CT than CS when exposed to cold treatment. Histochemical analyses indicated that roots of CS genotype present higher levels of lipid peroxidation and H2O2 accumulation, along with lower levels of plasma membrane integrity than CT under low temperature stress. RNAseq analyses revealed that the contrasting genotypes present completely different molecular responses to cold stress. The number of over-represented functional categories was lower in CT than CS under cold condition, suggesting that CS genotype is more impacted by low temperature stress than CT. Several genes might contribute to rice cold tolerance, including the ones related with cell wall remodeling, cytoskeleton and growth, signaling, antioxidant system, lipid metabolism, and stress response. On the other hand, high expression of the genes SRC2 (defense), root architecture associated 1 (growth), ACC oxidase, ethylene-responsive transcription factor, and cytokinin-O-glucosyltransferase 2 (hormone-related) seems to be related with cold sensibility. Since these two genotypes have a similar genetic background (sister lines), the differentially expressed genes found here can be considered candidate genes for cold tolerance and could be used in future biotechnological approaches aiming to increase rice tolerance to low temperature.
Assuntos
Aclimatação/genética , Resposta ao Choque Frio/genética , Resposta ao Choque Frio/fisiologia , Oryza/genética , Oryza/fisiologia , Raízes de Plantas/genética , Raízes de Plantas/fisiologia , Aclimatação/fisiologia , Brasil , Regulação da Expressão Gênica de Plantas , Variação Genética , GenótipoRESUMO
Brown wheat mites, Petrobia latens (Müller 1776, Acari: Tetranychidae), are sporadic yet economically damaging pests of winter cereals. In Colorado, their life history is closely tied to the development of winter wheat, where they are present in the field from crop planting in late September through harvest in early June. In order to withstand winter months, these mites are able to survive cold temperatures. However, the mechanisms of cold hardening and their temperature limits are unknown. This research documents the seasonal supercooling points of the brown wheat mite. Their seasonal average supercooling point stayed consistent throughout the year, never varying more than a degree from the overall average supercooling point of -17°C. The greatest variation in supercooling point was seen in the spring, during which supercooling point temperatures ranged from -9.2 to -25.5°C. We also documented the upper and lower lethal temperatures for the brown wheat mite. When comparing small nymphs to large nymph and adult stages, small nymphs were slightly more cold tolerant (lethal temperature estimates required to kill 99% of the population [LT99] were -30.8 and -30.6°C, respectively), but less heat tolerant (LT99 was 50 and 56°C, respectively).
Assuntos
Ácaros , Triticum , Animais , Temperatura Baixa , Colorado , Estações do Ano , TemperaturaRESUMO
Understanding the genetic basis of cold tolerance is a key step towards obtaining new and improved crop varieties. Current geographical distribution of durum wheat in Argentina exposes the plants to frost damage when spikes have already emerged. Biochemical pathways involved in cold tolerance are known to be early activated at above freezing temperatures. In this study we reported the transcriptome of CBW0101 spring durum wheat by merging data from untreated control and cold (5 °C) treated plant samples at reproductive stage. A total of 128,804 unigenes were predicted. Near 62% of the unigenes were annotated in at least one database. In total 876 unigenes were differentially expressed (DEGs), 562 were up-regulated and 314 down-regulated in treated samples. DEGs are involved in many critical processes including, photosynthetic activity, lipid and carbohydrate synthesis and accumulation of amino acids and seed proteins. Twenty-eight transcription factors (TFs) belonging to 14 families resulted differentially expressed from which eight families comprised of only TFs induced by cold. We also found 31 differentially expressed Long non-coding RNAs (lncRNAs), most of them up-regulated in treated plants. Two of these lncRNAs could operate via microRNAs (miRNAs) target mimic. Our results suggest a reprogramming of expression patterns in CBW0101 that affects a number of genes that is closer to the number reported in winter genotypes. These observations could partially explain its moderate tolerance (low proportion of frost-damaged spikes) when exposed to freezing days in the field.
Assuntos
Resposta ao Choque Frio/genética , Triticum/genética , Triticum/metabolismo , Argentina , Temperatura Baixa , Resposta ao Choque Frio/fisiologia , Congelamento , Regulação da Expressão Gênica de Plantas/genética , Genes de Plantas/genética , Genótipo , MicroRNAs/genética , RNA Longo não Codificante/metabolismo , Fatores de Transcrição/genética , Transcriptoma/genéticaRESUMO
Two strategies have been described for cold tolerance in arthropods: (1) freeze-tolerant organisms, which can survive the formation of ice crystals and (2) freeze-avoidant organisms, which prevent the ice crystal formation by super cooling their internal fluids. We studied two arthropods from the Andean Range in central Chile (2400â¯m a.s.l.), the stick insect Agathemera crassa commonly named as "Chinchemolle", and the tarantula spider Euathlus condorito commonly named as "Araña pollito", in order to evaluate how they respond to low temperatures at the physiological and molecular levels. We sampled the soil temperature during one year to track the temperature changes that these organisms must overcome. We found minimum temperatures around -6⯰C in autumn, while the temperature were stable at 0⯰C in winter due to the snow. The average field-cooling rate was 0.01⯱â¯0.006⯰Câ¯min-1. For both arthropods we determined the super cooling point (SCP) at a cooling rate of 1⯰Câ¯min-1 and its subsequent survival, finding that A. crassa is a freezing tolerant organism with a SCP of -3.8⯱â¯1.8⯰C and 100% survival, while E. condorito is a freezing avoidant organism with a SCP of -3.0⯱â¯1.3⯰C and 0% survival. The SCP and survival were not affected by the season in which individuals were collected, the SCP was significantly affected by the cooling rate of the experiment. Both species had low molecular weight cryoprotective in their hemolymph that could explain their cold-tolerance behavior. Glucose, glycerol, and trehalose were found in A. crassa's hemolymph, only glucose and glycerol were found in E. condorito's. We analyzed the hemolymph proteins and found no seasonal differences in composition for either species and also we detected protein antifreeze activity in the hemolymph from both arthropods.
Assuntos
Neópteros/fisiologia , Aranhas/fisiologia , Aclimatação , Animais , Proteínas Anticongelantes/metabolismo , Chile , Temperatura Baixa , Hemolinfa/metabolismo , Proteínas de Insetos/metabolismo , SoloRESUMO
BACKGROUND: Low temperature influences the development and latex production of rubber trees (Hevea brasiliensis) when extension to suboptimal high-latitude areas. The successful extension of Hevea brasiliensis cultivation to high-latitude areas has long believed to benefit from the breeding of cold-tolerant cultivars. A puzzling incongruity is the variation in cold tolerance among the cultivated clones despite their similar genetic make-up. RESULTS: To investigate this, we first transferred cultivar Reyan 7-33-97 to short-term cold treatment, and showed that cold-related genes (such as HbICE1 and HbCBF2), cold-responsive (COR) genes, and DNA-methylation related genes (such as HbMET1) were induced by cold treatment. Furthermore, long-term cold treatment not only elevated the transcriptional activities of the HbICE1, HbCBF2, and HbMET, but also induced DNA demethylation of their promoters. Cold treatment increased the transcriptional activities of demethylation-related genes such as the HbDME, HbROS, and HbDML genes, but did not alter the promoter methylation status. Furthermore, the HbICE1 and HbMET promoters showed hypomethylation status in samples collected at the end of winter from 12 different cultivars grown in four geographical locations, but switched to hypermethylation status at the end of summer. Expression of COR was correlated with the low temperature. Given that little genetic diversity exists in the HbICE1 and HbMET promoters among different cultivars, the DNA demethylation induced by cold was highly correlated with low temperature, but not with the genetic backgrounds of cultivars. CONCLUSION: Cold-induced epigenetic modification might play an important role in cold tolerance of H. brasiliensis.
Assuntos
Desmetilação do DNA , Hevea/fisiologia , Temperatura Baixa , Resposta ao Choque Frio , Ilhas de CpG/genética , Metilação de DNA , Regulação da Expressão Gênica de Plantas , Genes de Plantas/genética , Genes de Plantas/fisiologia , Geografia , Hevea/genética , Regiões Promotoras Genéticas/genética , Estações do Ano , Análise de Sequência de DNA , TranscriptomaRESUMO
KEY MESSAGE: Cold-tolerance in rice may be related to increased cellulose deposition in the cell wall, membrane fatty acids unsaturation and differential expression of several newly identified genes. Low temperature exposure during early vegetative stages limits rice plant's growth and development. Most genes previously related to cold tolerance in rice are from the japonica subspecies. To help clarify the mechanisms that regulate cold tolerance in young indica rice plants, comparative transcriptome analysis of 6 h cold-treated (10 °C) leaves from two genotypes, cold-tolerant (CT) and cold-sensitive (CS), was performed. Differentially expressed genes were identified: 831 and 357 sequences more expressed in the tolerant and in the sensitive genotype, respectively. The genes with higher expression in the CT genotype were used in systems biology analyses to identify protein-protein interaction (PPI) networks and nodes (proteins) that are hubs and bottlenecks in the PPI. From the genes more expressed in the tolerant plants, 60% were reported as affected by cold in previous transcriptome experiments and 27% are located within QTLs related to cold tolerance during the vegetative stage. Novel cold-responsive genes were identified. Quantitative RT-PCR confirmed the high-quality of RNAseq libraries. Several genes related to cell wall assembly or reinforcement are cold-induced or constitutively highly expressed in the tolerant genotype. Cold-tolerant plants have increased cellulose deposition under cold. Genes related to lipid metabolism are more expressed in the tolerant genotype, which has higher membrane fatty acids unsaturation, with increasing levels of linoleic acid under cold. The CT genotype seems to have higher photosynthetic efficiency and antioxidant capacity, as well as more effective ethylene, Ca2+ and hormone signaling than the CS. These genes could be useful in future biotechnological approaches aiming to increase cold tolerance in rice.
Assuntos
Adaptação Fisiológica/genética , Temperatura Baixa , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Oryza/genética , Perfilação da Expressão Gênica/métodos , Regulação da Expressão Gênica no Desenvolvimento , Regulação da Expressão Gênica de Plantas , Genes de Plantas/genética , Genótipo , Oryza/crescimento & desenvolvimento , Folhas de Planta/genética , Folhas de Planta/crescimento & desenvolvimento , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Mapas de Interação de Proteínas/genética , Locos de Características Quantitativas/genética , Plântula/genética , Plântula/crescimento & desenvolvimentoRESUMO
The natural composition of nutrients present in food is a key factor determining the immune function and stress responses in the honeybee (Apis mellifera). We previously demonstrated that a supplement of abscisic acid (ABA), a natural component of nectar, pollen, and honey, increases honeybee colony survival overwinter. Here we further explored the role of ABA in in vitro-reared larvae exposed to low temperatures. Four-day-old larvae (L4) exposed to 25°C for 3 days showed lower survival rates and delayed development compared to individuals growing at a standard temperature (34°C). Cold-stressed larvae maintained higher levels of ABA for longer than do larvae reared at 34°C, suggesting a biological significance for ABA. Larvae fed with an ABA-supplemented diet completely prevent the low survival rate due to cold stress and accelerate adult emergence. ABA modulates the expression of genes involved in metabolic adjustments and stress responses: Hexamerin 70b, Insulin Receptor Substrate, Vitellogenin, and Heat Shock Proteins 70. AmLANCL2, the honeybee ABA receptor, is also regulated by cold stress and ABA. These results support a role for ABA increasing the tolerance of honeybee larvae to low temperatures through priming effects.
Assuntos
Ácido Abscísico/administração & dosagem , Abelhas/fisiologia , Temperatura Baixa , Animais , Larva/fisiologiaRESUMO
The annual Zea mays ssp. mexicana L. is a member of teosinte, a wild relative of the Zea mays spp. mays L. This subspecies has strong growth and regeneration ability, high tiller numbers, high protein and lysine content as well as resistance to many fungal diseases, and it can be effectively used in maize improvement. In this study, we reported a Zea mays ssp. mexicana L. transcriptome by merging data from untreated control (CK), cold (4°C) and drought (PEG2000, 20%) treated plant samples. A total of 251,145 transcripts (N50 = 1,269 bp) and 184,280 unigenes (N50 = 923 bp) were predicted, which code for homologs of near 47% of the published maize proteome. Under cold conditions, 2,232 and 817 genes were up-regulated and down-regulated, respectively, while fewer genes were up-regulated (532) and down-regulated (82) under drought stress, indicating that Zea mays ssp. mexicana L. is more sensitive to the applied cold rather than to the applied drought stresses. Functional enrichment analyses identified many common or specific biological processes and gene sets in response to drought and cold stresses. The ABA dependent pathway, trehalose synthetic pathway and the ICE1-CBF pathway were up-regulated by both stresses. GA associated genes have been shown to differentially regulate the responses to cold in close subspecies in Zea mays. These findings and the identified functional genes can provide useful clues for improving abiotic stress tolerance of maize.
RESUMO
Rice productivity is largely affected by low temperature, which can be harmful throughout plant development, from germination to grain filling. Germination of indica rice cultivars under cold is slow and not uniform, resulting in irregular emergence and small plant population. To identify and characterize novel genes involved in cold tolerance during the germination stage, two indica rice genotypes (sister lines previously identified as cold-tolerant and cold-sensitive) were used in parallel transcriptomic analysis (RNAseq) under cold treatment (seeds germinating at 13 °C for 7 days). We detected 1,361 differentially expressed transcripts. Differences in gene expression found by RNAseq were confirmed for 11 selected genes using RT-qPCR. Biological processes enhanced in the cold-tolerant seedlings include: cell division and expansion (confirmed by anatomical sections of germinating seeds), cell wall integrity and extensibility, water uptake and membrane transport capacity, sucrose synthesis, generation of simple sugars, unsaturation of membrane fatty acids, wax biosynthesis, antioxidant capacity (confirmed by histochemical staining of H2O2), and hormone and Ca(2+)-signaling. The cold-sensitive seedlings respond to low temperature stress increasing synthesis of HSPs and dehydrins, along with enhanced ubiquitin/proteasome protein degradation pathway and polyamine biosynthesis. Our findings can be useful in future biotechnological approaches aiming to cold tolerance in indica rice.
Assuntos
Adaptação Fisiológica/genética , Temperatura Baixa , Germinação/genética , Sequenciamento de Nucleotídeos em Larga Escala , Oryza/genética , Oryza/fisiologia , Sementes/genética , Análise de Sequência de RNA , Divisão Celular , Perfilação da Expressão Gênica , Regulação da Expressão Gênica no Desenvolvimento , Regulação da Expressão Gênica de Plantas , Biblioteca Gênica , Ontologia Genética , Genótipo , Peróxido de Hidrogênio/metabolismo , Oryza/citologia , Reação em Cadeia da Polimerase em Tempo Real , Reprodutibilidade dos Testes , Plântula/citologia , Plântula/genética , Transcriptoma/genéticaRESUMO
BACKGROUND: Changed temperature not only threaten agricultural production, but they also affect individual biological behavior, population and community of many insects, and consequently reduce the stability of our ecosystem. Insect's ability to respond to temperature stress evolved through a complex adaptive process, thus resulting in varied temperature tolerance among different insects. Both high and low extreme temperatures are detrimental to insect development since they constitute an important abiotic stress capable of inducing abnormal biological responses. Many studies on heat or cold tolerance of ladybirds have focused on measurements of physiological and biochemical indexes such as supercooling point, higher/lower lethal temperatures, survival rate, dry body weight, water content, and developmental duration. And studies of the molecular mechanisms of ladybird responses to heat or cold stress have focused on single genes, such as those encoding heat shock proteins, but has not been analyzed by transcriptome profiling. RESULTS: In this study, we report the use of Digital Gene Expression (DGE) tag profiling to gain insight into transcriptional events associated with heat- and cold-stress in C. montrouzieri. About 6 million tags (49 bp in length) were sequenced in a heat stress group, a cold stress group and a negative control group. We obtained 687 and 573 genes that showed significantly altered expression levels following heat and cold shock treatments, respectively. Analysis of the global gene expression pattern suggested that 42 enzyme-encoding genes mapped to many Gene Ontology terms are associated with insect's response to heat- and cold-stress. CONCLUSIONS: These results provide a global assessment of genes and molecular mechanisms involved in heat and cold tolerance.
Assuntos
Animais , Besouros/genética , Resposta ao Choque Térmico/genética , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Resposta ao Choque Frio/genética , Transcriptoma , Estresse Fisiológico/genética , Besouros/classificação , Besouros/enzimologia , Biblioteca Gênica , Análise de Sequência de DNA/métodos , Genes de Insetos/fisiologia , Temperatura Baixa , Primers do DNA , Perfilação da Expressão Gênica/métodos , Reação em Cadeia da Polimerase em Tempo Real , Ontologia Genética , Temperatura AltaRESUMO
Low temperatures at the initial stages of rice development prevent fast germination and seedling establishment and may cause significant productivity losses. In order to develop rice cultivars exhibiting cold tolerance, it is necessary to investigate genetic resources, providing basic knowledge to allow the introduction of genes involved in low temperature germination ability from accessions into elite cultivars. Japanese rice accessions were evaluated at the germination under two conditions: 13°C for 28 days (cold stress) and 28°C for seven days (optimal temperature). The traits studied were coleoptile and radicle length under optimal temperature, coleoptile and radicle length under cold and percentage of the reduction in coleptile and radicle length due to low temperature. Among the accessions studied, genetic variation for traits related to germination under low temperatures was observed and accessions exhibiting adequate performance for all investigated traits were identified. The use of multivariate analysis allowed the identification of the genotypes displaying cold tolerance by smaller reductions in coleoptile and radicle lenght in the presence of cold and high vigour, by higher coleoptile and radicle growth under cold.
RESUMO
O presente trabalho teve como objetivo avaliar a resposta das espécies arbóreas Schizolobium parahyba (Vell.) Blake (guapuruvu), Mimosa scabrella Benth. (bracatinga), Peltophorum dubium (Spr.) Taubert (canafístula), Parapiptadenia rigida (Benth.) Brenan (angico-vermelho) e o híbrido Eucalyptus urophylla S.T. Blake x Eucalyptus grandis Hill ex Maiden (eucalipto) em consórcio com a cana-de-açúcar (Saccharum spp.) em dois arranjos de sistemas agroflorestais (faixa - 3x3m + 12m e linha - 6x1,5m) submetidos a extremos climáticos de geada, na região Noroeste do Rio Grande do Sul. Os danos (resistência á geada) foram avaliados segundo o sistema de notas utilizado por HIGA et al. (2000) no qual atribuiu-se uma nota de 0 a 10 conforme a intensidade do dano na planta. Para os graus de resistência à geada, adaptou-se metodologia utilizada por CARVALHO (1981), a qual também é em função da intensidade do dano na planta. Diante da condição de geada estudada, observa-se que os diferentes arranjos de sistemas agroflorestais afetam a resistência da espécie guapuruvu, sendo sensível no sistema agroflorestal faixa e moderadamente tolerante no sistema linha. Em ambos os sistemas, as espécies angico-vermelho, bracatinga e eucalipto mostram-se resistentes, enquanto que a canafístula demonstra ser tolerante.
The present research had the aim to evaluate the answers of the arboreal species Schizolobium parahyba (Vell.) Blake (guapuruvu), Mimosa scabrella Benth. (bracatinga), Peltophorum dubium (Spr.) Taubert (canafístula), Parapiptadenia rigida (Benth.) Brenan (angico-vermelho) and the hybrid Eucalyptus urophylla S.T. Blake x Eucalyptus grandis Hill ex Maiden (eucalipto) in consortium with sugar cane (Saccharum spp.) in two agroforestry arrangement systems (strip - 3x3m + 12m and line - 6x1,5m) submitted to climatic extreme frost conditions, in the Northwest of Rio Grande do Sul. The damages (resistance to frost) were appraised according to the system of notes used by HIGA et al. (2000) in which a note from 0 to 10 was attributed according to the intensity of the damage to the plant. For the resistance degrees to frost, it was adapted the methodology used by CARVALHO (1981), which is also in function of the intensity of the damage to the plant. Upon the frost condition studied, it was observed that the results demonstrated that different of agroforest system arrangements damaged the resistance of the guapuruvu specie, being sensitive in the agroforest system strip and moderatelly tolerant in the system line. In both systems, the species angico-vermelho, bracatinga e eucalipto showed resistance, while the canafístula demonstrated to be tolerant.
RESUMO
The present research had the aim to evaluate the answers of the arboreal species Schizolobium parahyba (Vell.) Blake (guapuruvu), Mimosa scabrella Benth. (bracatinga), Peltophorum dubium (Spr.) Taubert (canafístula), Parapiptadenia rigida (Benth.) Brenan (angico-vermelho) and the hybrid Eucalyptus urophylla S.T. Blake x Eucalyptus grandis Hill ex Maiden (eucalipto) in consortium with sugar cane (Saccharum spp.) in two agroforestry arrangement systems (strip - 3x3m + 12m and line - 6x1,5m) submitted to climatic extreme frost conditions, in the Northwest of Rio Grande do Sul. The damages (resistance to frost) were appraised according to the system of notes used by HIGA et al. (2000) in which a note from 0 to 10 was attributed according to the intensity of the damage to the plant. For the resistance degrees to frost, it was adapted the methodology used by CARVALHO (1981), which is also in function of the intensity of the damage to the plant. Upon the frost condition studied, it was observed that the results demonstrated that different of agroforest system arrangements damaged the resistance of the guapuruvu specie, being sensitive in the agroforest system strip and moderatelly tolerant in the system line. In both systems, the species angico-vermelho, bracatinga e eucalipto showed resistance, while the canafístula demonstrated to be tolerant.
O presente trabalho teve como objetivo avaliar a resposta das espécies arbóreas Schizolobium parahyba (Vell.) Blake (guapuruvu), Mimosa scabrella Benth. (bracatinga), Peltophorum dubium (Spr.) Taubert (canafístula), Parapiptadenia rigida (Benth.) Brenan (angico-vermelho) e o híbrido Eucalyptus urophylla S.T. Blake x Eucalyptus grandis Hill ex Maiden (eucalipto) em consórcio com a cana-de-açúcar (Saccharum spp.) em dois arranjos de sistemas agroflorestais (faixa - 3x3m + 12m e linha - 6x1,5m) submetidos a extremos climáticos de geada, na região Noroeste do Rio Grande do Sul. Os danos (resistência á geada) foram avaliados segundo o sistema de notas utilizado por HIGA et al. (2000) no qual atribuiu-se uma nota de 0 a 10 conforme a intensidade do dano na planta. Para os graus de resistência à geada, adaptou-se metodologia utilizada por CARVALHO (1981), a qual também é em função da intensidade do dano na planta. Diante da condição de geada estudada, observa-se que os diferentes arranjos de sistemas agroflorestais afetam a resistência da espécie guapuruvu, sendo sensível no sistema agroflorestal faixa e moderadamente tolerante no sistema linha. Em ambos os sistemas, as espécies angico-vermelho, bracatinga e eucalipto mostram-se resistentes, enquanto que a canafístula demonstra ser tolerante.
RESUMO
The present research had the aim to evaluate the answers of the arboreal species Schizolobium parahyba (Vell.) Blake (guapuruvu), Mimosa scabrella Benth. (bracatinga), Peltophorum dubium (Spr.) Taubert (canafístula), Parapiptadenia rigida (Benth.) Brenan (angico-vermelho) and the hybrid Eucalyptus urophylla S.T. Blake x Eucalyptus grandis Hill ex Maiden (eucalipto) in consortium with sugar cane (Saccharum spp.) in two agroforestry arrangement systems (strip - 3x3m + 12m and line - 6x1,5m) submitted to climatic extreme frost conditions, in the Northwest of Rio Grande do Sul. The damages (resistance to frost) were appraised according to the system of notes used by HIGA et al. (2000) in which a note from 0 to 10 was attributed according to the intensity of the damage to the plant. For the resistance degrees to frost, it was adapted the methodology used by CARVALHO (1981), which is also in function of the intensity of the damage to the plant. Upon the frost condition studied, it was observed that the results demonstrated that different of agroforest system arrangements damaged the resistance of the guapuruvu specie, being sensitive in the agroforest system strip and moderatelly tolerant in the system line. In both systems, the species angico-vermelho, bracatinga e eucalipto showed resistance, while the canafístula demonstrated to be tolerant.
O presente trabalho teve como objetivo avaliar a resposta das espécies arbóreas Schizolobium parahyba (Vell.) Blake (guapuruvu), Mimosa scabrella Benth. (bracatinga), Peltophorum dubium (Spr.) Taubert (canafístula), Parapiptadenia rigida (Benth.) Brenan (angico-vermelho) e o híbrido Eucalyptus urophylla S.T. Blake x Eucalyptus grandis Hill ex Maiden (eucalipto) em consórcio com a cana-de-açúcar (Saccharum spp.) em dois arranjos de sistemas agroflorestais (faixa - 3x3m + 12m e linha - 6x1,5m) submetidos a extremos climáticos de geada, na região Noroeste do Rio Grande do Sul. Os danos (resistência á geada) foram avaliados segundo o sistema de notas utilizado por HIGA et al. (2000) no qual atribuiu-se uma nota de 0 a 10 conforme a intensidade do dano na planta. Para os graus de resistência à geada, adaptou-se metodologia utilizada por CARVALHO (1981), a qual também é em função da intensidade do dano na planta. Diante da condição de geada estudada, observa-se que os diferentes arranjos de sistemas agroflorestais afetam a resistência da espécie guapuruvu, sendo sensível no sistema agroflorestal faixa e moderadamente tolerante no sistema linha. Em ambos os sistemas, as espécies angico-vermelho, bracatinga e eucalipto mostram-se resistentes, enquanto que a canafístula demonstra ser tolerante.
RESUMO
The present research had the aim to evaluate the answers of the arboreal species Schizolobium parahyba (Vell.) Blake (guapuruvu), Mimosa scabrella Benth. (bracatinga), Peltophorum dubium (Spr.) Taubert (canafístula), Parapiptadenia rigida (Benth.) Brenan (angico-vermelho) and the hybrid Eucalyptus urophylla S.T. Blake x Eucalyptus grandis Hill ex Maiden (eucalipto) in consortium with sugar cane (Saccharum spp.) in two agroforestry arrangement systems (strip - 3x3m + 12m and line - 6x1,5m) submitted to climatic extreme frost conditions, in the Northwest of Rio Grande do Sul. The damages (resistance to frost) were appraised according to the system of notes used by HIGA et al. (2000) in which a note from 0 to 10 was attributed according to the intensity of the damage to the plant. For the resistance degrees to frost, it was adapted the methodology used by CARVALHO (1981), which is also in function of the intensity of the damage to the plant. Upon the frost condition studied, it was observed that the results demonstrated that different of agroforest system arrangements damaged the resistance of the guapuruvu specie, being sensitive in the agroforest system strip and moderatelly tolerant in the system line. In both systems, the species angico-vermelho, bracatinga e eucalipto showed resistance, while the canafístula demonstrated to be tolerant.
O presente trabalho teve como objetivo avaliar a resposta das espécies arbóreas Schizolobium parahyba (Vell.) Blake (guapuruvu), Mimosa scabrella Benth. (bracatinga), Peltophorum dubium (Spr.) Taubert (canafístula), Parapiptadenia rigida (Benth.) Brenan (angico-vermelho) e o híbrido Eucalyptus urophylla S.T. Blake x Eucalyptus grandis Hill ex Maiden (eucalipto) em consórcio com a cana-de-açúcar (Saccharum spp.) em dois arranjos de sistemas agroflorestais (faixa - 3x3m + 12m e linha - 6x1,5m) submetidos a extremos climáticos de geada, na região Noroeste do Rio Grande do Sul. Os danos (resistência á geada) foram avaliados segundo o sistema de notas utilizado por HIGA et al. (2000) no qual atribuiu-se uma nota de 0 a 10 conforme a intensidade do dano na planta. Para os graus de resistência à geada, adaptou-se metodologia utilizada por CARVALHO (1981), a qual também é em função da intensidade do dano na planta. Diante da condição de geada estudada, observa-se que os diferentes arranjos de sistemas agroflorestais afetam a resistência da espécie guapuruvu, sendo sensível no sistema agroflorestal faixa e moderadamente tolerante no sistema linha. Em ambos os sistemas, as espécies angico-vermelho, bracatinga e eucalipto mostram-se resistentes, enquanto que a canafístula demonstra ser tolerante.
RESUMO
A produtividade de uma pastagem perene tropical, em regiões de clima temperado, é dependente de seu comportamento em relação às condições de temperatura. A avaliação da sensibilidade das plantas, através da sua exposição a temperaturas inferiores a 2ºC em ambiente controlado, pode ser um procedimento bastante eficiente na predição de resistência, em função de assegurar uma homogeneidade dos níveis de frio. Considerando-se à reduzida disponibilidade de trabalhos científicos relacionados com a avaliação de leguminosas forrageiras tropicais quanto à tolerância ao frio, este experimento teve como objetivo avaliar o comportamento do amendoim forrageiro (cv. "Amarillo") sob temperaturas inferiores a 2ºC. O delineamento experimental adotado foi completamente casualizado com 15 repetições e dois tratamentos, com exposição (CE) e sem exposição ao frio (SE). Os caracteres mensurados foram: número de folhas por estolho, espessura do estolho e número de brotações novas. Os resultados indicaram que a exposição do amendoim forrageiro a um intervalo de temperatura de -1,0 a 1,3ºC por um período de 3 horas é capaz de causar estresse de frio nas plantas, provocando uma redução no número de folhas e estimulando a formação de novas brotações, sem provocar a morte das plantas.(AU)
The yield of tropical perennial forages in temperate climate areas depends on its cold tolerance. The exposure of genotypes to temperatures below 2oC, under controlled conditions is an efficient methodology to predict cold tolerance, since it maintains homogeneous levels of cold. Due to absence of information related to cold tolerance of tropical forages, this experiment was developed aiming to evaluate the behavior of forage peanut exposed to temperatures below 2oC. The design adopted was completely randomized with 15 replications and two treatments: exposed and not exposed to cold. The traits measured were: number of leaves per stolon; thickness of stolon and number of new shoots. The results showed that the exposition of the forage peanut to a range of temperature from -1.0 to 1.3 ºC, for 3 hours, causes cold stress in the plants, resulting in a reduction of the number of leaves per solon and stimulating the emergency of new shoots, but did not kill the plants.(AU)
Assuntos
Arachis , Temperatura BaixaRESUMO
A produtividade de uma pastagem perene tropical, em regiões de clima temperado, é dependente de seu comportamento em relação às condições de temperatura. A avaliação da sensibilidade das plantas, através da sua exposição a temperaturas inferiores a 2°C em ambiente controlado, pode ser um procedimento bastante eficiente na predição de resistência, em função de assegurar uma homogeneidade dos níveis de frio. Considerando-se à reduzida disponibilidade de trabalhos científicos relacionados com a avaliação de leguminosas forrageiras tropicais quanto à tolerância ao frio, este experimento teve como objetivo avaliar o comportamento do amendoim forrageiro (cv. "Amarillo") sob temperaturas inferiores a 2°C. O delineamento experimental adotado foi completamente casualizado com 15 repetições e dois tratamentos, com exposição (CE) e sem exposição ao frio (SE). Os caracteres mensurados foram: número de folhas por estolho, espessura do estolho e número de brotações novas. Os resultados indicaram que a exposição do amendoim forrageiro a um intervalo de temperatura de -1,0 a 1,3°C por um período de 3 horas é capaz de causar estresse de frio nas plantas, provocando uma redução no número de folhas e estimulando a formação de novas brotações, sem provocar a morte das plantas.
The yield of tropical perennial forages in temperate climate areas depends on its cold tolerance. The exposure of genotypes to temperatures below 2oC, under controlled conditions is an efficient methodology to predict cold tolerance, since it maintains homogeneous levels of cold. Due to absence of information related to cold tolerance of tropical forages, this experiment was developed aiming to evaluate the behavior of forage peanut exposed to temperatures below 2oC. The design adopted was completely randomized with 15 replications and two treatments: exposed and not exposed to cold. The traits measured were: number of leaves per stolon; thickness of stolon and number of new shoots. The results showed that the exposition of the forage peanut to a range of temperature from -1.0 to 1.3 °C, for 3 hours, causes cold stress in the plants, resulting in a reduction of the number of leaves per solon and stimulating the emergency of new shoots, but did not kill the plants.