RESUMO
Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) of the gastrointestinal tract. The etiology is not fully understood, but environmental, microbial, and immunologic factors, as well as a genetic predisposition, play a role. UC is characterized by episodes of abdominal pain, diarrhea, bloody stools, weight loss, severe colonic inflammation, and ulceration. Despite the increase in the frequency of UC and the deterioration of the quality of life, there are still patients who do not respond well to available treatment options. Against this background, natural products such as polysaccharides are becoming increasingly important as they protect the intestinal mucosa, promote wound healing, relieve inflammation and pain, and restore intestinal motility. In this study, we investigated the effect of a polysaccharide isolated from the biomass of Campomanesia adamantium and Campomanesia pubescens (here referred to as CPW) in an experimental model of acute and chronic ulcerative colitis induced by dextran sulfate sodium (DSS). CPW reversed weight loss, increased disease activity index (DAI), bloody diarrhea, and colon shortening. In addition, CPW reduced visceral mechanical hypersensitivity, controlled oxidative stress and inflammation, and protected the mucosal barrier. CPW is not absorbed in the intestine, does not inhibit cytochrome P450 proteins, and does not exhibit AMES toxicity. These results suggest that CPW attenuates DSS-induced acute and chronic colitis in mice and may be a potential alternative treatment for UC.
Assuntos
Colite Ulcerativa , Humanos , Animais , Camundongos , Colite Ulcerativa/induzido quimicamente , Colite Ulcerativa/tratamento farmacológico , Qualidade de Vida , Modelos Animais de Doenças , Inflamação , Redução de Peso , DiarreiaRESUMO
Inflammatory bowel disease (IBD) includes two distinct diseases: Crohn's disease (CD) and ulcerative colitis (UC). IBD is a chronic systemic disease of the gastrointestinal tract, characterized by an inflammatory process. The mechanisms by which diseases develop are still unknown, but it is known that it results from a complex interaction between genetic variability, the host's immune system, and environmental factors. One of the main complaints of patients is abdominal pain, which may be associated with the release of inflammatory mediators, changes in the normal motility of the digestive tract, and increased intestinal permeability. Currently available drugs for abdominal pain are not satisfactory, therefore, it is extremely necessary to seek new therapeutic options for the treatment of abdominal pain. Polysaccharides extracted from fruits have attracted interest, as these molecules protect the intestinal mucosa and promote wound healing, attenuating inflammation, pain, and altered intestinal motility. In this study, we investigated the ability of pectic polysaccharides obtained from guavira pomace, named CPW to reduce visceral hypersensitivity, regulate intestinal motility, and control diarrhea in mice. Acetic acid, capsaicin, or mustard oil were used to assess visceral pain in normal mice. CPW reduced abdominal writhing, cell migration, and capsaicin-induced visceral nociception. Furthermore, it regulated intestinal motility and all measured parameters of castor oil-induced diarrhea. CPW treatment reversed the increase in mucosal permeability, TEER, and tissue weight caused by acetic acid. In addition, molecular docking analysis showed that specific the CPW units binds to the 3N8V, 5COX, 2J67 and 6RBF proteins. Thus, the results suggest that CPW has attractive therapeutic characteristics for the treatment of abdominal pain and ulcerative colitis.
Assuntos
Colite Ulcerativa , Doenças Inflamatórias Intestinais , Dor Abdominal/tratamento farmacológico , Animais , Capsaicina , Colite Ulcerativa/tratamento farmacológico , Diarreia , Carboidratos da Dieta , Frutas , Humanos , Camundongos , Simulação de Acoplamento Molecular , Polissacarídeos/farmacologiaRESUMO
Exotic fruits and their co-products may be valuable sources of antioxidant dietary fibres (DF) which are useful for food industry and human health. In this study, we aimed to characterize DF obtained from guavira fruit pomace and investigate its antioxidant potential employing TEAC assay as well as a cell model. The DF were chemically characterized as containing arabinan, highly-methoxylated homogalacturonan and arabinogalactan. The DF-containing fraction (CPW) presented ABTS free radical scavenger activity. MTT and DCFH-DA assay were performed to assess, respectively, changes in cell viability and the potential intracellular antioxidant activity against H2O2-induced oxidative stress in murine NIH 3T3 fibroblast. CPW exhibited no effects on cell viability, moreover, when administered 48 h prior the induction of H2O2 toxic effects, it protected the cells, significantly increasing the cell viability compared to control. This protection may be related to the observed reduction of reactive oxygen species levels. Thus, the pre-treatment of cells with guavira DF for 48 h remarkably induced a cytoprotection against pro-oxidant conditions, and may be a valuable functional compound recovered from an unexploited agroindutrial waste.
Assuntos
Antioxidantes/análise , Antioxidantes/farmacologia , Fibras na Dieta/análise , Frutas/química , Animais , Sobrevivência Celular/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Galactanos/química , Peróxido de Hidrogênio/toxicidade , Camundongos , Células NIH 3T3 , Estresse Oxidativo , Pectinas/química , Polissacarídeos/químicaRESUMO
Guavira is a plant that belongs to Myrtaceae family, being widespread in the Brazilian Cerrado. In this study, pectic and hemicellulosic polysaccharides from guavira pomace, an agroindustry residue from pulp production, were structurally characterized using GPC, monosaccharide composition, methylation and NMR experiments. The absolute configurations of monosaccharides and the nature of uronic acids were attributed according to numerous data on the composition of related plant arabinogalactans and hemicelluloses present in the literature. An arabinan was purified, presenting Ara (85.0%), Rha (3.3%), Gal (7.7%) and GalA (4.0%). Mono and bidimensional NMR analyses of this arabinan demonstrated the presence of terminal ß-L-Araf units, whose occurrence has been scarcely reported in the literature. Hemicellulosic fraction contained a glucuronoxylan, with α-D-GlcpA/4-O-methyl-α-D-GlcpA group linked to O-2 of a (1â¯ââ¯4)-ß-D-xylan, presenting one uronic acid residue for every six xylose units. These findings about guavira pomace polysaccharides could contribute to develop future nutraceutical and technological uses for this industrial waste.
Assuntos
Frutas/química , Myrtaceae/química , Polissacarídeos/química , Xilanos/química , Polissacarídeos/isolamento & purificação , Xilanos/isolamento & purificaçãoRESUMO
Gabiroba marmalades were prepared with coconut, pectin, citric acid anddifferent concentrations of glucose syrup (0%, 5%, 10% and 15%) using a1:1:1 ratio of pulp:sugar:milk, for evaluation of physico-chemical propertiesand acceptability. The formulations with acceptable sensory attributeswere stored at room temperature and monthly evaluated for physical andchemical characteristics (pH, titrable acidity, soluble solids and moisture)until completing 180 days. The overall acceptability (aroma, taste, texture)and appearance averaged 6.2 ? 7.8 in a 9-point hedonic scale consumeracceptance study. During storage of marmalades, a tendency for decrease in the pH value was observed, this is also indicated by the negative linear effect (p < 0.05). The variation in the values of titrable acidity, soluble solids and moisture was not linear or quadratic during storage. Gabiroba marmalades with increasing levels of glucose syrup (0-15%) and good level of acceptance present adequate physical and chemical characteristics.
Mermelada de gabiroba fue preparado usando una proporción de pulpa:azucar: leche de 1: 1: 1 respectivamente, coco rallado, pectina,ácido cítrico y diferentes concentraciones de jarabe de glucosa (0, 5, 10 y 15%) para evaluar las características físico-químicas y su aceptabilidad. Las formulaciones aceptadas fueron almacenadas a temperatura ambiente y evaluadas mensualmente en relación a sus características físico-químicas (pH, acidez, sólidos solubles y humedad) hasta completar 180 días. Las puntuaciones de aceptación en cuanto a aroma, sabor, textura y apariencia del producto variaron de 6,2 a 7,8, utilizando unaescala hedónica de 9 puntos. Durante el almacenamiento, los mermeladas mostraron tendencia de reducción del pH, confi rmado por el efecto linear negativo en función del tiempo (p < 0,05). La variación en los valores de acidez titulable, sólidos solubles y humedad no fue linear ni cuadrática con el tiempo de almacenamiento. Mermeladas de gabiroba conniveles crecientes de jarabe de glucosa (0 hasta 15%) y buen nivel de aceptación presentaron características físico-químicas adecuadas.
Doces em pasta de gabiroba foram elaborados com a proporção de polpa:açúcar:leite de 1:1:1, coco ralado, pectina, ácido cítrico e diferentesconcentrações de xarope de glicose (0, 5, 10; 15%) para avaliação das características físicoquímicas e da aceitabilidade. As formulaçõesconsideradas aceitas foram arazenadas à temperatura ambiente e avaliadas quanto às características físicas e químicas (pH, acideztitulável, sólidos solúveis e umidade), mensalmente até completar 180 dias. Os escores para aceitação global (aroma, sabor, textura) e aparência dosdoces variaram de 6,2 a 7,8 usando-se escala hedônica de 9 pontos. Durante a estocagem, os doces apresentaram tendência na redução dopH, isto também é confi rmado pelo efeito linear negativo em função do tempo (p<0,05). A alteração dos valores de acidez titulável, sólidos solúveis e umidade não foi linear ou quadrática com o tempo de armazenamento. Doces de gabiroba com níveis crescentes dexarope de glicose (0% a 15%) e bom nível de aceitação apresentam características físicas e químicas adequadas.