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1.
PeerJ ; 12: e17686, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39006015

RESUMO

In the present investigation, we employ a novel and meticulously structured database assembled by experts, encompassing macrofungi field-collected in Brazil, featuring upwards of 13,894 photographs representing 505 distinct species. The purpose of utilizing this database is twofold: firstly, to furnish training and validation for convolutional neural networks (CNNs) with the capacity for autonomous identification of macrofungal species; secondly, to develop a sophisticated mobile application replete with an advanced user interface. This interface is specifically crafted to acquire images, and, utilizing the image recognition capabilities afforded by the trained CNN, proffer potential identifications for the macrofungal species depicted therein. Such technological advancements democratize access to the Brazilian Funga, thereby enhancing public engagement and knowledge dissemination, and also facilitating contributions from the populace to the expanding body of knowledge concerning the conservation of macrofungal species of Brazil.


Assuntos
Aprendizado Profundo , Fungos , Brasil , Fungos/classificação , Fungos/isolamento & purificação , Biodiversidade , Redes Neurais de Computação , Bases de Dados Factuais
2.
Med Biol Eng Comput ; 2024 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-39028484

RESUMO

Stroke is a neurological condition that usually results in the loss of voluntary control of body movements, making it difficult for individuals to perform activities of daily living (ADLs). Brain-computer interfaces (BCIs) integrated into robotic systems, such as motorized mini exercise bikes (MMEBs), have been demonstrated to be suitable for restoring gait-related functions. However, kinematic estimation of continuous motion in BCI systems based on electroencephalography (EEG) remains a challenge for the scientific community. This study proposes a comparative analysis to evaluate two artificial neural network (ANN)-based decoders to estimate three lower-limb kinematic parameters: x- and y-axis position of the ankle and knee joint angle during pedaling tasks. Long short-term memory (LSTM) was used as a recurrent neural network (RNN), which reached Pearson correlation coefficient (PCC) scores close to 0.58 by reconstructing kinematic parameters from the EEG features on the delta band using a time window of 250 ms. These estimates were evaluated through kinematic variance analysis, where our proposed algorithm showed promising results for identifying pedaling and rest periods, which could increase the usability of classification tasks. Additionally, negative linear correlations were found between pedaling speed and decoder performance, thereby indicating that kinematic parameters between slower speeds may be easier to estimate. The results allow concluding that the use of deep learning (DL)-based methods is feasible for the estimation of lower-limb kinematic parameters during pedaling tasks using EEG signals. This study opens new possibilities for implementing controllers most robust for MMEBs and BCIs based on continuous decoding, which may allow for maximizing the degrees of freedom and personalized rehabilitation.

3.
Sensors (Basel) ; 24(12)2024 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-38931751

RESUMO

This work addresses the challenge of classifying multiclass visual EEG signals into 40 classes for brain-computer interface applications using deep learning architectures. The visual multiclass classification approach offers BCI applications a significant advantage since it allows the supervision of more than one BCI interaction, considering that each class label supervises a BCI task. However, because of the nonlinearity and nonstationarity of EEG signals, using multiclass classification based on EEG features remains a significant challenge for BCI systems. In the present work, mutual information-based discriminant channel selection and minimum-norm estimate algorithms were implemented to select discriminant channels and enhance the EEG data. Hence, deep EEGNet and convolutional recurrent neural networks were separately implemented to classify the EEG data for image visualization into 40 labels. Using the k-fold cross-validation approach, average classification accuracies of 94.8% and 89.8% were obtained by implementing the aforementioned network architectures. The satisfactory results obtained with this method offer a new implementation opportunity for multitask embedded BCI applications utilizing a reduced number of both channels (<50%) and network parameters (<110 K).


Assuntos
Algoritmos , Interfaces Cérebro-Computador , Aprendizado Profundo , Eletroencefalografia , Redes Neurais de Computação , Eletroencefalografia/métodos , Humanos , Processamento de Sinais Assistido por Computador
4.
Sensors (Basel) ; 24(3)2024 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-38339433

RESUMO

Around 70 million people worldwide are affected by epilepsy, a neurological disorder characterized by non-induced seizures that occur at irregular and unpredictable intervals. During an epileptic seizure, transient symptoms emerge as a result of extreme abnormal neural activity. Epilepsy imposes limitations on individuals and has a significant impact on the lives of their families. Therefore, the development of reliable diagnostic tools for the early detection of this condition is considered beneficial to alleviate the social and emotional distress experienced by patients. While the Bonn University dataset contains five collections of EEG data, not many studies specifically focus on subsets D and E. These subsets correspond to EEG recordings from the epileptogenic zone during ictal and interictal events. In this work, the parallel ictal-net (PIN) neural network architecture is introduced, which utilizes scalograms obtained through a continuous wavelet transform to achieve the high-accuracy classification of EEG signals into ictal or interictal states. The results obtained demonstrate the effectiveness of the proposed PIN model in distinguishing between ictal and interictal events with a high degree of confidence. This is validated by the computing accuracy, precision, recall, and F1 scores, all of which consistently achieve around 99% confidence, surpassing previous approaches in the related literature.


Assuntos
Eletroencefalografia , Epilepsia , Humanos , Eletroencefalografia/métodos , Convulsões/diagnóstico , Epilepsia/diagnóstico , Redes Neurais de Computação , Análise de Ondaletas
5.
Ciênc. rural (Online) ; 54(1): e20220677, 2024. ilus, graf, tab
Artigo em Inglês | VETINDEX | ID: biblio-1438078

RESUMO

Multivariate time series forecasting has an important role in many real-world domains. Especially, price prediction has always been on the focus of researchers. Yet, it is a challenging task that requires the capturing of intra-series and inter-series correlations. Most of the models in literature focus only on the correlation in temporal domain. In this paper, we have curated a new dataset from the official website of Turkish Ministry of Commerce. The dataset consists of daily prices and trade volume of vegetables and covers 1791 days between January 1, 2018 and November 26, 2022. A Spectral Temporal Graph Neural Network (StemGNN) is employed on the curated dataset and the results are given in comparison to Convolutional neural networks (CNN), Long short-term memory (LSTM) and Random Forest models. GNN architecture achieved a state-of-the-art result such as mean absolute error (MAE): 1,37 and root mean squared error (RMSE): 1.94). To our knowledge, this is one of the few studies that investigates GNN for time series analysis and the first study in architecture field.


A previsão multivariada de séries temporais tem um papel importante em muitos domínios do mundo real. Especialmente, a previsão de preços sempre esteve no foco dos pesquisadores. No entanto, é uma tarefa desafiadora que requer a captura de correlações intra-séries e inter-séries. A maioria dos modelos na literatura foca apenas a correlação no domínio temporal. Neste artigo, selecionamos um novo conjunto de dados do site oficial do Ministério do Comércio Turco. O conjunto de dados consiste em preços diários e volume comercial de vegetais e abrange 1.791 dias entre 1º de janeiro de 2018 e 26 de novembro de 2022. Uma Rede Neural de Gráfico Temporal Espectral é empregada no conjunto de dados curado e os resultados são fornecidos em comparação com CNN, LSTM e Modelos de Floresta Aleatória. A arquitetura GNN alcançou um resultado de ponta (MAE: 1,37, RMSE: 1,94). Até onde sabemos, este é um dos poucos estudos que investiga GNN para análise de séries temporais e o primeiro estudo na área de arquitetura.


Assuntos
Fatores de Tempo , Comércio , Agricultura/economia
6.
Sensors (Basel) ; 23(22)2023 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-38005600

RESUMO

Detecting people in images and videos captured from an aerial platform in wooded areas for search and rescue operations is a current problem. Detection is difficult due to the relatively small dimensions of the person captured by the sensor in relation to the environment. The environment can generate occlusion, complicating the timely detection of people. There are currently numerous RGB image datasets available that are used for person detection tasks in urban and wooded areas and consider the general characteristics of a person, like size, shape, and height, without considering the occlusion of the object of interest. The present research work focuses on developing a thermal image dataset, which considers the occlusion situation to develop CNN convolutional deep learning models to perform detection tasks in real-time from an aerial perspective using altitude control in a quadcopter prototype. Extended models are proposed considering the occlusion of the person, in conjunction with a thermal sensor, which allows for highlighting the desired characteristics of the occluded person.

7.
Sensors (Basel) ; 23(17)2023 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-37688015

RESUMO

In recent years, the application of artificial intelligence (AI) in the automotive industry has led to the development of intelligent systems focused on road safety, aiming to improve protection for drivers and pedestrians worldwide to reduce the number of accidents yearly. One of the most critical functions of these systems is pedestrian detection, as it is crucial for the safety of everyone involved in road traffic. However, pedestrian detection goes beyond the front of the vehicle; it is also essential to consider the vehicle's rear since pedestrian collisions occur when the car is in reverse drive. To contribute to the solution of this problem, this research proposes a model based on convolutional neural networks (CNN) using a proposed one-dimensional architecture and the Inception V3 architecture to fuse the information from the backup camera and the distance measured by the ultrasonic sensors, to detect pedestrians when the vehicle is reversing. In addition, specific data collection was performed to build a database for the research. The proposed model showed outstanding results with 99.85% accuracy and 99.86% correct classification performance, demonstrating that it is possible to achieve the goal of pedestrian detection using CNN by fusing two types of data.

8.
Bioengineering (Basel) ; 10(5)2023 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-37237599

RESUMO

Even with over 80% of the population being vaccinated against COVID-19, the disease continues to claim victims. Therefore, it is crucial to have a secure Computer-Aided Diagnostic system that can assist in identifying COVID-19 and determining the necessary level of care. This is especially important in the Intensive Care Unit to monitor disease progression or regression in the fight against this epidemic. To accomplish this, we merged public datasets from the literature to train lung and lesion segmentation models with five different distributions. We then trained eight CNN models for COVID-19 and Common-Acquired Pneumonia classification. If the examination was classified as COVID-19, we quantified the lesions and assessed the severity of the full CT scan. To validate the system, we used Resnetxt101 Unet++ and Mobilenet Unet for lung and lesion segmentation, respectively, achieving accuracy of 98.05%, F1-score of 98.70%, precision of 98.7%, recall of 98.7%, and specificity of 96.05%. This was accomplished in just 19.70 s per full CT scan, with external validation on the SPGC dataset. Finally, when classifying these detected lesions, we used Densenet201 and achieved accuracy of 90.47%, F1-score of 93.85%, precision of 88.42%, recall of 100.0%, and specificity of 65.07%. The results demonstrate that our pipeline can correctly detect and segment lesions due to COVID-19 and Common-Acquired Pneumonia in CT scans. It can differentiate these two classes from normal exams, indicating that our system is efficient and effective in identifying the disease and assessing the severity of the condition.

9.
Vaccines (Basel) ; 11(2)2023 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-36851190

RESUMO

Monkeypox is a rare disease caused by the monkeypox virus. This disease was considered eradicated in 1980 and was believed to affect rodents and not humans. However, recent years have seen a massive outbreak of monkeypox in humans, setting off worldwide alerts from health agencies. As of September 2022, the number of confirmed cases in Peru had reached 1964. Although most monkeypox patients have been discharged, we cannot neglect the monitoring of the population with respect to the monkeypox virus. Lately, the population has started to express their feelings and opinions through social media, specifically Twitter, as it is the most used social medium and is an ideal space to gather what people think about the monkeypox virus. The information imparted through this medium can be in different formats, such as text, videos, images, audio, etc. The objective of this work is to analyze the positive, negative, and neutral feelings of people who publish their opinions on Twitter with the hashtag #Monkeypox. To find out what people think about this disease, a hybrid-based model architecture built on CNN and LSTM was used to determine the prediction accuracy. The prediction result obtained from the total monkeypox data was 83% accurate. Other performance metrics were also used to evaluate the model, such as specificity, recall level, and F1 score, representing 99%, 85%, and 88%, respectively. The results also showed the polarity of feelings through the CNN-LSTM confusion matrix, where 45.42% of people expressed neither positive nor negative opinions, while 19.45% expressed negative and fearful feelings about this infectious disease. The results of this work contribute to raising public awareness about the monkeypox virus.

10.
Artigo em Inglês | MEDLINE | ID: mdl-36673883

RESUMO

Falling events are a global health concern with short- and long-term physical and psychological implications, especially for the elderly population. This work aims to monitor human activity in an indoor environment and recognize falling events without requiring users to carry a device or sensor on their bodies. A sensing platform based on the transmission of a continuous wave (CW) radio-frequency (RF) probe signal was developed using general-purpose equipment. The CW probe signal is similar to the pilot subcarriers transmitted by commercial off-the-shelf WiFi devices. As a result, our methodology can easily be integrated into a joint radio sensing and communication scheme. The sensing process is carried out by analyzing the changes in phase, amplitude, and frequency that the probe signal suffers when it is reflected or scattered by static and moving bodies. These features are commonly extracted from the channel state information (CSI) of WiFi signals. However, CSI relies on complex data acquisition and channel estimation processes. Doppler radars have also been used to monitor human activity. While effective, a radar-based fall detection system requires dedicated hardware. In this paper, we follow an alternative method to characterize falling events on the basis of the Doppler signatures imprinted on the CW probe signal by a falling person. A multi-class deep learning framework for classification was conceived to differentiate falling events from other activities that can be performed in indoor environments. Two neural network models were implemented. The first is based on a long-short-term memory network (LSTM) and the second on a convolutional neural network (CNN). A series of experiments comprising 11 subjects were conducted to collect empirical data and test the system's performance. Falls were detected with an accuracy of 92.1% for the LSTM case, while for the CNN, an accuracy rate of 92.1% was obtained. The results demonstrate the viability of human fall detection based on a radio sensing system such as the one described in this paper.


Assuntos
Aprendizado Profundo , Humanos , Idoso , Redes Neurais de Computação , Radar , Atividades Humanas
11.
F1000Res ; 12: 14, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-38826575

RESUMO

Background: Glaucoma and diabetic retinopathy (DR) are the leading causes of irreversible retinal damage leading to blindness. Early detection of these diseases through regular screening is especially important to prevent progression. Retinal fundus imaging serves as the principal method for diagnosing glaucoma and DR. Consequently, automated detection of eye diseases represents a significant application of retinal image analysis. Compared with classical diagnostic techniques, image classification by convolutional neural networks (CNN) exhibits potential for effective eye disease detection. Methods: This paper proposes the use of MATLAB - retrained AlexNet CNN for computerized eye diseases identification, particularly glaucoma and diabetic retinopathy, by employing retinal fundus images. The acquisition of the database was carried out through free access databases and access upon request. A transfer learning technique was employed to retrain the AlexNet CNN for non-disease (Non_D), glaucoma (Sus_G) and diabetic retinopathy (Sus_R) classification. Moreover, model benchmarking was conducted using ResNet50 and GoogLeNet architectures. A Grad-CAM analysis is also incorporated for each eye condition examined. Results: Metrics for validation accuracy, false positives, false negatives, precision, and recall were reported. Validation accuracies for the NetTransfer (I-V) and netAlexNet ranged from 89.7% to 94.3%, demonstrating varied effectiveness in identifying Non_D, Sus_G, and Sus_R categories, with netAlexNet achieving a 93.2% accuracy in the benchmarking of models against netResNet50 at 93.8% and netGoogLeNet at 90.4%. Conclusions: This study demonstrates the efficacy of using a MATLAB-retrained AlexNet CNN for detecting glaucoma and diabetic retinopathy. It emphasizes the need for automated early detection tools, proposing CNNs as accessible solutions without replacing existing technologies.


Assuntos
Retinopatia Diabética , Glaucoma , Redes Neurais de Computação , Humanos , Retinopatia Diabética/diagnóstico por imagem , Retinopatia Diabética/diagnóstico , Glaucoma/diagnóstico , Glaucoma/diagnóstico por imagem , Inteligência Artificial
12.
Diagnostics (Basel) ; 12(12)2022 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-36553037

RESUMO

Glaucoma is an eye disease that gradually deteriorates vision. Much research focuses on extracting information from the optic disc and optic cup, the structure used for measuring the cup-to-disc ratio. These structures are commonly segmented with deeplearning techniques, primarily using Encoder-Decoder models, which are hard to train and time-consuming. Object detection models using convolutional neural networks can extract features from fundus retinal images with good precision. However, the superiority of one model over another for a specific task is still being determined. The main goal of our approach is to compare object detection model performance to automate segment cups and discs on fundus images. This study brings the novelty of seeing the behavior of different object detection models in the detection and segmentation of the disc and the optical cup (Mask R-CNN, MS R-CNN, CARAFE, Cascade Mask R-CNN, GCNet, SOLO, Point_Rend), evaluated on Retinal Fundus Images for Glaucoma Analysis (REFUGE), and G1020 datasets. Reported metrics were Average Precision (AP), F1-score, IoU, and AUCPR. Several models achieved the highest AP with a perfect 1.000 when the threshold for IoU was set up at 0.50 on REFUGE, and the lowest was Cascade Mask R-CNN with an AP of 0.997. On the G1020 dataset, the best model was Point_Rend with an AP of 0.956, and the worst was SOLO with 0.906. It was concluded that the methods reviewed achieved excellent performance with high precision and recall values, showing efficiency and effectiveness. The problem of how many images are needed was addressed with an initial value of 100, with excellent results. Data augmentation, multi-scale handling, and anchor box size brought improvements. The capability to translate knowledge from one database to another shows promising results too.

13.
Diagnostics (Basel) ; 12(10)2022 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-36292051

RESUMO

Osteoarthritis (OA) affects nearly 240 million people worldwide. Knee OA is the most common type of arthritis, especially in older adults. Physicians measure the severity of knee OA according to the Kellgren and Lawrence (KL) scale through visual inspection of X-ray or MR images. We propose a semi-automatic CADx model based on Deep Siamese convolutional neural networks and a fine-tuned ResNet-34 to simultaneously detect OA lesions in the two knees according to the KL scale. The training was done using a public dataset, whereas the validations were performed with a private dataset. Some problems of the imbalanced dataset were solved using transfer learning. The model results average of the multi-class accuracy is 61%, presenting better performance results for classifying classes KL-0, KL-3, and KL-4 than KL-1 and KL-2. The classification results were compared and validated using the classification of experienced radiologists.

14.
Cancers (Basel) ; 14(16)2022 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-36010850

RESUMO

We conducted a systematic review and meta-analysis of the diagnostic performance of current deep learning algorithms for the diagnosis of lung cancer. We searched major databases up to June 2022 to include studies that used artificial intelligence to diagnose lung cancer, using the histopathological analysis of true positive cases as a reference. The quality of the included studies was assessed independently by two authors based on the revised Quality Assessment of Diagnostic Accuracy Studies. Six studies were included in the analysis. The pooled sensitivity and specificity were 0.93 (95% CI 0.85−0.98) and 0.68 (95% CI 0.49−0.84), respectively. Despite the significantly high heterogeneity for sensitivity (I2 = 94%, p < 0.01) and specificity (I2 = 99%, p < 0.01), most of it was attributed to the threshold effect. The pooled SROC curve with a bivariate approach yielded an area under the curve (AUC) of 0.90 (95% CI 0.86 to 0.92). The DOR for the studies was 26.7 (95% CI 19.7−36.2) and heterogeneity was 3% (p = 0.40). In this systematic review and meta-analysis, we found that when using the summary point from the SROC, the pooled sensitivity and specificity of DL algorithms for the diagnosis of lung cancer were 93% and 68%, respectively.

15.
Front Nutr ; 9: 901333, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35928831

RESUMO

Sweetener type can influence sensory properties and consumer's acceptance and preference for low-calorie products. An ideal sweetener does not exist, and each sweetener must be used in situations to which it is best suited. Aspartame and sucralose can be good substitutes for sucrose in passion fruit juice. Despite the interest in artificial sweeteners, little is known about how artificial sweeteners are processed in the human brain. Here, we applied the convolutional neural network (CNN) to evaluate brain signals of 11 healthy subjects when they tasted passion fruit juice equivalently sweetened with sucrose (9.4 g/100 g), sucralose (0.01593 g/100 g), or aspartame (0.05477 g/100 g). Electroencephalograms were recorded for two sites in the gustatory cortex (i.e., C3 and C4). Data with artifacts were disregarded, and the artifact-free data were used to feed a Deep Neural Network with tree branches that applied a Convolutions and pooling for different feature filtering and selection. The CNN received raw signal as input for multiclass classification and with supervised training was able to extract underling features and patterns from the signal with better performance than handcrafted filters like FFT. Our results indicated that CNN is an useful tool for electroencephalography (EEG) analyses and classification of perceptually similar tastes.

16.
Sensors (Basel) ; 22(15)2022 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-35957287

RESUMO

COVID-19, the illness caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus belonging to the Coronaviridade family, a single-strand positive-sense RNA genome, has been spreading around the world and has been declared a pandemic by the World Health Organization. On 17 January 2022, there were more than 329 million cases, with more than 5.5 million deaths. Although COVID-19 has a low mortality rate, its high capacities for contamination, spread, and mutation worry the authorities, especially after the emergence of the Omicron variant, which has a high transmission capacity and can more easily contaminate even vaccinated people. Such outbreaks require elucidation of the taxonomic classification and origin of the virus (SARS-CoV-2) from the genomic sequence for strategic planning, containment, and treatment of the disease. Thus, this work proposes a high-accuracy technique to classify viruses and other organisms from a genome sequence using a deep learning convolutional neural network (CNN). Unlike the other literature, the proposed approach does not limit the length of the genome sequence. The results show that the novel proposal accurately distinguishes SARS-CoV-2 from the sequences of other viruses. The results were obtained from 1557 instances of SARS-CoV-2 from the National Center for Biotechnology Information (NCBI) and 14,684 different viruses from the Virus-Host DB. As a CNN has several changeable parameters, the tests were performed with forty-eight different architectures; the best of these had an accuracy of 91.94 ± 2.62% in classifying viruses into their realms correctly, in addition to 100% accuracy in classifying SARS-CoV-2 into its respective realm, Riboviria. For the subsequent classifications (family, genera, and subgenus), this accuracy increased, which shows that the proposed architecture may be viable in the classification of the virus that causes COVID-19.


Assuntos
COVID-19 , SARS-CoV-2 , Humanos , Redes Neurais de Computação , Pandemias , SARS-CoV-2/genética
17.
Front Med (Lausanne) ; 9: 894430, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35712087

RESUMO

Background: Deep learning methods have demonstrated remarkable performance in pathology image analysis, but they are computationally very demanding. The aim of our study is to reduce their computational cost to enable their use with large tissue image datasets. Methods: We propose a method called Network Auto-Reduction (NAR) that simplifies a Convolutional Neural Network (CNN) by reducing the network to minimize the computational cost of doing a prediction. NAR performs a compound scaling in which the width, depth, and resolution dimensions of the network are reduced together to maintain a balance among them in the resulting simplified network. We compare our method with a state-of-the-art solution called ResRep. The evaluation is carried out with popular CNN architectures and a real-world application that identifies distributions of tumor-infiltrating lymphocytes in tissue images. Results: The experimental results show that both ResRep and NAR are able to generate simplified, more efficient versions of ResNet50 V2. The simplified versions by ResRep and NAR require 1.32× and 3.26× fewer floating-point operations (FLOPs), respectively, than the original network without a loss in classification power as measured by the Area under the Curve (AUC) metric. When applied to a deeper and more computationally expensive network, Inception V4, NAR is able to generate a version that requires 4× lower than the original version with the same AUC performance. Conclusions: NAR is able to achieve substantial reductions in the execution cost of two popular CNN architectures, while resulting in small or no loss in model accuracy. Such cost savings can significantly improve the use of deep learning methods in digital pathology. They can enable studies with larger tissue image datasets and facilitate the use of less expensive and more accessible graphics processing units (GPUs), thus reducing the computing costs of a study.

18.
Genes (Basel) ; 13(5)2022 05 19.
Artigo em Inglês | MEDLINE | ID: mdl-35627292

RESUMO

Many living organisms have DNA in their cells that is responsible for their biological features. DNA is an organic molecule of two complementary strands of four different nucleotides wound up in a double helix. These nucleotides are adenine (A), thymine (T), guanine (G), and cytosine (C). Genes are DNA sequences containing the information to synthesize proteins. The genes of higher eukaryotic organisms contain coding sequences, known as exons and non-coding sequences, known as introns, which are removed on splice sites after the DNA is transcribed into RNA. Genome annotation is the process of identifying the location of coding regions and determining their function. This process is fundamental for understanding gene structure; however, it is time-consuming and expensive when done by biochemical methods. With technological advances, splice site detection can be done computationally. Although various software tools have been developed to predict splice sites, they need to improve accuracy and reduce false-positive rates. The main goal of this research was to generate Deep Splicer, a deep learning model to identify splice sites in the genomes of humans and other species. This model has good performance metrics and a lower false-positive rate than the currently existing tools. Deep Splicer achieved an accuracy between 93.55% and 99.66% on the genetic sequences of different organisms, while Splice2Deep, another splice site detection tool, had an accuracy between 90.52% and 98.08%. Splice2Deep surpassed Deep Splicer on the accuracy obtained after evaluating C. elegans genomic sequences (97.88% vs. 93.62%) and A. thaliana (95.40% vs. 94.93%); however, Deep Splicer's accuracy was better for H. sapiens (98.94% vs. 97.15%) and D. melanogaster (97.14% vs. 92.30%). The rate of false positives was 0.11% for human genetic sequences and 0.25% for other species' genetic sequences. Another splice prediction tool, Splice Finder, had between 1% and 3% of false positives for human sequences, while other species' sequences had around 4% and 10%.


Assuntos
Caenorhabditis elegans , Drosophila melanogaster , Animais , Caenorhabditis elegans/genética , DNA/genética , Drosophila melanogaster/genética , Humanos , Nucleotídeos , Software
19.
Appl Energy ; 313: 118848, 2022 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-35250149

RESUMO

This paper proposes a time-series stochastic socioeconomic model for analyzing the impact of the pandemic on the regulated distribution electricity market. The proposed methodology combines the optimized tariff model (socioeconomic market model) and the random walk concept (risk assessment technique) to ensure robustness/accuracy. The model enables both a past and future analysis of the impact of the pandemic, which is essential to prepare regulatory agencies beforehand and allow enough time for the development of efficient public policies. By applying it to six Brazilian concession areas, results demonstrate that consumers have been/will be heavily affected in general, mainly due to the high electricity tariffs that took place with the pandemic, overcoming the natural trend of the market. In contrast, the model demonstrates that the pandemic did not/will not significantly harm power distribution companies in general, mainly due to the loan granted by the regulator agency, named COVID-account. Socioeconomic welfare losses averaging 500 (MR$/month) are estimated for the equivalent concession area, i.e., the sum of the six analyzed concession areas. Furthermore, this paper proposes a stochastic optimization problem to mitigate the impact of the pandemic on the electricity market over time, considering the interests of consumers, power distribution companies, and the government. Results demonstrate that it is successful as the tariffs provided by the algorithm compensate for the reduction in demand while increasing the socioeconomic welfare of the market.

20.
Sensors (Basel) ; 22(4)2022 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-35214571

RESUMO

Single image depth estimation works fail to separate foreground elements because they can easily be confounded with the background. To alleviate this problem, we propose the use of a semantic segmentation procedure that adds information to a depth estimator, in this case, a 3D Convolutional Neural Network (CNN)-segmentation is coded as one-hot planes representing categories of objects. We explore 2D and 3D models. Particularly, we propose a hybrid 2D-3D CNN architecture capable of obtaining semantic segmentation and depth estimation at the same time. We tested our procedure on the SYNTHIA-AL dataset and obtained σ3=0.95, which is an improvement of 0.14 points (compared with the state of the art of σ3=0.81) by using manual segmentation, and σ3=0.89 using automatic semantic segmentation, proving that depth estimation is improved when the shape and position of objects in a scene are known.


Assuntos
Processamento de Imagem Assistida por Computador , Semântica , Redes Neurais de Computação
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