RESUMO
Porous structured surfaces decorated with gold nanoparticles can be fabricated in an economical two-step process. Sub-micrometric porous polymethyl methacrylate (PMMA) templates are formed by using the breath-figure method (BFM) with a single-step spin-coating onto a conducting substrate to result in a set of delimited microdomains for gold electrodeposition. By controlling electrochemical parameters, distinct morphologies are engendered, among them, nanoparticles that present useful local Raman enhancement in the order of up to 106 with stability of a solid-phase platform and characteristic chemical resistance of gold. The spatial confinement of metallic structures resulted in electromagnetic field enhancement, here compared to flat metallic surfaces where contributions of area effect and fluorescence quenching are responsible for a significant apparent enhancement to Raman spectra that has not been frequently reported to date.
RESUMO
A facile and reproducible route that can lead to two-dimensional arrays of nanopores in thin polymer films is demonstrated. The formation of the pores in the polymer films involves breath figure phenomenon and occurs during the film deposition by spin coating. The formation of nanoporous thin films takes only few seconds, and the method does not require complex equipment or expensive chemicals. This method also constitutes a straightforward approach to control the size of the pores formed in thin films. Besides allowing control over the average pore size of the porous films, the use of dynamic deposition with the breath figure phenomenon causes the reduction in the pore size to nanometer scale. The nanoporous arrays obtained by the breath figure are applied as substrates for cell growth, and the effect of their nanopore size on cell growth was evaluated. Notably, it is found that cell viability is related to pore size, where 2D nanoporous structure is more beneficial for cell culture than 2D microporous structures. The change in the average pore size of the polymer films from 1.22 µm to 346 nm results in a threefold increase in cell viability.