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Dolphin-Assisted Therapies (DAT) are alternative therapies aimed to reduce anxiety levels, stress relief and physical benefits. This paper is focused on measuring and analyzing dolphins brain activity when DAT is taking place in order to identify if there is any differences in female dolphin's neuronal signal when it is interacting with control or intervention subjects, performing our research in Delfiniti, Ixtapa, Mexico facilities. We designed a wireless and portable electroencephalographic single-channel signal capture sensor to acquire and monitor the brain activity of a female bottle-nose dolphin. This EEG sensor was able to show that dolphin activity at rest is characterized by high spectral power at slow-frequencies bands. When the dolphin participated in DAT, a 23.53% increment in the 12-30 Hz frequency band was observed, but this only occurred for patients with some disease or disorder, given that 0.5-4 Hz band keeps it at 17.91% when there is a control patient. Regarding the fractal or Self-Affine Analysis, we found for all samples studied that at the beginning the dolphin's brain activity behaved as a self-affine fractal described by a power-law until the fluctuations of voltage reached the crossovers, and after the crossovers these fluctuations left this scaling behavior. Hence, our findings validate the hypothesis that the participation in a DAT of a Patient with a certain disease or disorder modifies the usual behavior of a female bottle-nose dolphin.

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Tursiops truncatus gephyreus is only found in the inshore waters of the Southwestern Atlantic Ocean. They are organized in small socially, structured groups, which lead to low genetic variability and high anthropogenic threats. Currently, the information about their reproductive biology and management is still insufficient. Thus, this study aims to present novel information recorded for 20 years regarding reproductive parameters in a small population of captive T. t. gephyreus. Three of the animals involved were found stranded in La Plata River estuary and, the other two were born at the oceanarium. Thirteen semen evaluations showed high-quality semen with a low percentage of sperm abnormalities. Twelve pregnancies and parturitions were observed. There was a clear calving seasonality during austral spring and summer. The duration of phase-2 of labor was approximately 80 min, being the caudal presentation the most frequent. The average inter-birth interval (IBI) was about 33 months. This is the first report that closely monitored parturition, IBI, and seminal evaluation in this species. Information gathered during this program will allow the development of adequate conservation plans for free-ranging populations.

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Odontocetes are apex predators that, despite accumulating mercury (Hg) to high concentrations in their tissues, show few signs of Hg toxicity. One method of Hg detoxification in odontocetes includes the sequestering of Hg in toxicologically inert mercury selenide (HgSe) compounds. To explore the tissue-specific accumulation of Hg and Se and the potential protective role of Se against Hg toxicity, we measured the concentrations of total mercury (THg) and selenium (Se) in multiple tissues from 11 species of odontocetes that stranded along the northern Gulf of Mexico coast [Florida (FL) and Louisiana (LA)]. Tissues were collected primarily from bottlenose dolphins (Tursiops truncatus; n = 93); however, individuals from species in the following 8 genera were also sampled: Feresa (n = 1), Globicephala (n = 1), Grampus (n = 2), Kogia (n = 5), Mesoplodon (n = 1), Peponocephala (n = 4), Stenella (n = 9), and Steno (n = 1). In all species, mean THg concentrations were greatest in the liver and lowest in the blubber, lung, or skin. In contrast, in most species, mean Se concentrations were greatest in the liver, lung, or skin, and lowest in the blubber. For all species combined, Se:Hg molar ratios decreased with increasing THg concentration in the blubber, kidney, liver, lung, and skin following an exponential decay relationship. In bottlenose dolphins, THg concentrations in the kidney, liver, and lung were significantly greater in FL dolphins compared to LA dolphins. On average, in bottlenose dolphins, Se:Hg molar ratios were approximately 1:1 in the liver and >1:1 in blubber, kidney, lung, and skin, suggesting that Se likely protects against Hg toxicity. However, more research is necessary to understand the variation in Hg accumulation within and among species and to assess how Hg, in combination with other environmental stressors, influences odontocete population health.

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Melioidosis is an emerging infectious disease of humans and animals caused by the bacterium Burkholderia pseudomallei and endemic in tropical regions, principally Southeast Asia and northern Australia. In September 2017, after Hurricane Maria impacted the Dolphin Discovery facility in the Federation of St. Kitts and Nevis, a juvenile male bottlenose dolphin (Tursiops truncatus) died within 96 hr of presenting with acute anorexia, lethargy, and respiratory distress. Histopathology demonstrated necrohemorrhagic bronchopneumonia, necrotizing hepatitis, splenitis, and lymphadenitis, with intralesional Gram-negative bacilli. B. pseudomallei was confirmed by bacteriological culture and DNA sequencing. This case emphasizes the challenges of melioidosis diagnosis, the importance of awareness for both early detection and efficacious treatment, and recognition in tropical regions where it has been either not reported or underreported. To the authors' knowledge, this is the first case of cetacean melioidosis in the Caribbean Islands, an often severe and fatal disease with increasing prevalence on the American continent.

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Spermatozoa interactions with the female reproductive tract and oocyte are regulated by surface molecules such as glycocalyx. The capacitation process comprises molecular and structural modifications which increase zona pellucida binding affinity. Lectins allowed us to describe glycocalyx changes during maturation, capacitation and acrosome reaction. This study had as its aim to identify lectin binding patterns using four lectins with different carbohydrate affinity in bottlenose dolphin (Tursiops truncatus) spermatozoa both before and after in vitro capacitation. Two semen samples from the same dolphin obtained on consecutive days were used, with four different lectin binding patterns becoming visible in both samples before and after capacitation. A highly stained equatorial segment with prolongations at the edges appeared as the most frequent pattern with Wheat germ agglutinin (WGA) in uncapacitated spermatozoa. However, it was homogeneously distributed over the acrosomal region after capacitation. Instead, the use of Peanut agglutinin (PNA) resulted in most spermatozoa showing high labelling in the acrosomal periphery region before capacitation and a homogeneous staining in the acrosomal region within the population of capacitated spermatozoa. Nevertheless, the most representative patterns with Concavalin A (ConA) and Aleuria aurantia agglutinin (AAA) lectins did not change before and after capacitation, labelling the acrosomal region periphery. These findings could contribute to the understanding of the reproductive biology of cetaceans and the improvement of sperm selection techniques.

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Spermatozoa interactions with the female reproductive tract and oocyte are regulated by surface molecules such as glycocalyx. The capacitation process comprises molecular and structural modifications which increase zona pellucida binding affinity. Lectins allowed us to describe glycocalyx changes during maturation, capacitation and acrosome reaction. This study had as its aim to identify lectin binding patterns using four lectins with different carbohydrate affinity in bottlenose dolphin (Tursiops truncatus) spermatozoa both before and after in vitro capacitation. Two semen samples from the same dolphin obtained on consecutive days were used, with four different lectin binding patterns becoming visible in both samples before and after capacitation. A highly stained equatorial segment with prolongations at the edges appeared as the most frequent pattern with Wheat germ agglutinin (WGA) in uncapacitated spermatozoa. However, it was homogeneously distributed over the acrosomal region after capacitation. Instead, the use of Peanut agglutinin (PNA) resulted in most spermatozoa showing high labelling in the acrosomal periphery region before capacitation and a homogeneous staining in the acrosomal region within the population of capacitated spermatozoa. Nevertheless, the most representative patterns with Concavalin A (ConA) and Aleuria aurantia agglutinin (AAA) lectins did not change before and after capacitation, labelling the acrosomal region periphery. These findings could contribute to the understanding of the reproductive biology of cetaceans and the improvement of sperm selection techniques.

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Spermatozoa interactions with the female reproductive tract and oocyte are regulated by surface molecules such as glycocalyx. The capacitation process comprises molecular and structural modifications which increase zona pellucida binding affinity. Lectins allowed us to describe glycocalyx changes during maturation, capacitation and acrosome reaction. This study had as its aim to identify lectin binding patterns using four lectins with different carbohydrate affinity in bottlenose dolphin (Tursiops truncatus) spermatozoa both before and after in vitro capacitation. Two semen samples from the same dolphin obtained on consecutive days were used, with four different lectin binding patterns becoming visible in both samples before and after capacitation. A highly stained equatorial segment with prolongations at the edges appeared as the most frequent pattern with Wheat germ agglutinin (WGA) in uncapacitated spermatozoa. However, it was homogeneously distributed over the acrosomal region after capacitation. Instead, the use of Peanut agglutinin (PNA) resulted in most spermatozoa showing high labelling in the acrosomal periphery region before capacitation and a homogeneous staining in the acrosomal region within the population of capacitated spermatozoa. Nevertheless, the most representative patterns with Concavalin A (ConA) and Aleuria aurantia agglutinin (AAA) lectins did not change before and after capacitation, labelling the acrosomal region periphery. These findings could contribute to the understanding of the reproductive biology of cetaceans and the improvement of sperm selection techniques.(AU)

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Una comunidad residente de bufeos costeros Tursiops truncatus ha sido estudiada de manera intermitente entre 2005 y 2018 en la punta de la península de Santa Elena, Ecuador (2°11' S & 81°0.7' W). Avistamientos oportunistas y dirigidos desde la playa y abordo de un bote con motor fuera de borda se realizaron a lo largo de 40 km de costa, contabilizándose un esfuerzo de 917.2 km de seguimiento en automóvil desde tierra y 707.4 km en bote por mar. El tamaño grupal promedio fue 5.31 delfines/grupo (SD = 1.97, rango 1-10), sin cambios significativos a lo largo del estudio. Desde tierra el tamaño grupal fue subestimado en promedio en 32 %. Esta comunidad de bufeos tiene actualmente solo nueve individuos, incluyendo seis adultos, un inmaduro y dos crías, siendo la comunidad más pequeña encontrada en el golfo de Guayaquil. La tasa de encuentro varió entre 0.03 delfines/km en la parte noroeste y 0.31 delfines/km en la parte sur, donde los delfines concentran sus actividades, posiblemente porque las actividades humanas ahí son menos intensas. Los delfines se distribuyeron generalmente entre los primeros 200 m de la orilla llegando hasta 1 200 m en la parte norte donde la profundidad es menor y las actividades portuaria y turística más intensas. La velocidad de los delfines fue significativamente más alta cuando fueron seguidos desde un bote a corta distancia (5.87 km/h) que cuando fueron monitoreados desde tierra (2.9 km/h) (P < 0.01), lo cual sugiere que el seguimiento en bote tuvo un impacto sobre el movimiento de los delfines. Un análisis clúster jerárquico mostró que los delfines tienen altos índices de asociación entre ellos (promedio 0.67, rango 0.01-1.0), indicando que es una comunidad altamente cohesionada. Adicionalmente muestran un alto nivel de residencia (índice de ocurrencia promedio de 0.62). Durante el estudio se identificaron dos amenazas principales, una pesquería con redes agalleras en la parte suroeste y un intenso tráfico de botes pesqueros en la parte noroeste. La mayoría del área de estudio actualmente es parte de un área costero-marina protegida creada en 2008, lo cual ofrece una oportunidad para la recuperación de esta comunidad de delfines. Debido a su fragilidad, se recomienda a las autoridades ambientales, entre otras cosas, eliminar potenciales fuentes de amenaza para los delfines como las redes de pesca, implementar un área de exclusión para artes de pesca y tráfico de botes pesqueros de 1 km de ancho desde la orilla y limitar la velocidad de todo tipo de embarcación a 10 nudos dentro del área protegida.

A resident community of bottlenose dolphins (Tursiops truncatus) has been studied irregularly between 2005 and 2018 around the tip of the Santa Elena Peninsula, Ecuador (2°11' S & 81°0.7' W). Opportunistic sightings and systematic surveys from the beach and at sea were carried out along 40 km of coast, accounting for 917.2 km of tracking by car from land and 707.4 km of boat tracking by sea. Average group size was 5.31 dolphins/group (SD = 1.97, range 1-10), with no significant changes throughout the study period. From land, however, the group size was underestimated 32 % on the average. This small bottlenose dolphin community currently has only nine individuals, including six adults, one immature and two calves, and is the smallest community within the Gulf of Guayaquil. The encounter rate ranged between 0.03 dolphins/km in the northwestern part and 0.32 dolphins/km in the South, where dolphins concentrate their activities, possibly because human activities are less intense there. Dolphins were generally distributed in the first 200 m from the shore, reaching up to 1 200 m in the Northern shallower part and where port and tourist activities concentrate. The dolphins' speed was significantly higher when they were followed from a boat at close range (5.87 km/h) than when they were monitored from the beach (2.9 km/h) (P < 0.01), which suggests that boat tracking had an effect on dolphin´s movements. Pairwise cluster analysis showed that animals from this community show high rates of association among each other (average 0.67, range 0.01-1.0), indicating that is a highly cohesive community. Dolphins also showed high level of residence (average occurrence index = 0.62). During the study, two main threats were identified, a gillnet fishery in the Southwestern part and an intense fishing boat traffic in the Northwestern. Most of the study area is currently part of a coastal-marine protected area created in 2008, which offers an opportunity for the recovery and conservation of this dolphin community. Given its fragility, we recommend the environmental authorities to address potential threats for this dolphin community by eliminating gillnets, implementing an exclusion zone for fishing gear and boat traffic of 1 km width from the shore and limit the speed of any type of vessel to 10 knots within the reserve.

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For highly mobile species that nevertheless show fine-scale patterns of population genetic structure, the relevant evolutionary mechanisms determining structure remain poorly understood. The bottlenose dolphin (Tursiops truncatus) is one such species, exhibiting complex patterns of genetic structure associated with local habitat dependence in various geographic regions. Here we studied bottlenose dolphin populations in the Gulf of California and Pacific Ocean off Baja California where habitat is highly structured to test associations between ecology, habitat dependence and genetic differentiation. We investigated population structure at a fine geographic scale using both stable isotope analysis (to assess feeding ecology) and molecular genetic markers (to assess population structure). Our results show that there are at least two factors affecting population structure for both genetics and feeding ecology (as indicated by stable isotope profiles). On the one hand there is a signal for the differentiation of individuals by ecotype, one foraging more offshore than the other. At the same time, there is differentiation between the Gulf of California and the west coast of Baja California, meaning that for example, nearshore ecotypes were both genetically and isotopically differentiated either side of the peninsula. We discuss these data in the context of similar studies showing fine-scale population structure for delphinid species in coastal waters, and consider possible evolutionary mechanisms.

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Clinical and epidemiological features of tattoo skin disease (TSD) are reported for 257 common bottlenose dolphins held in 31 facilities in the Northern Hemisphere. Photographs and biological data of 146 females and 111 males were analyzed. Dolphins were classified into three age classes: 0-3 years, 4-8 years, and older than 9 years. From 2012 to 2014, 20.6% of the 257 dolphins showed clinical TSD. The youngest dolphins with tattoo lesions were 14 and 15 months old. TSD persisted from 4 to 65 months in 30 dolphins. Prevalence varied between facilities from 5.6% to 60%, possibly reflecting variation in environmental factors. Unlike in free-ranging Delphinidae, TSD prevalence was significantly higher in males (31.5%) than in females (12.3%). Infection was age-dependent only in females. Prevalence of very large tattoos was also higher in males (28.6%) than in females (11.1%). These data suggest that male T. truncatus are more vulnerable to TSD than females, possibly because of differences in immune response and susceptibility to captivity-related stress.

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Common bottlenose dolphins serve as sentinels for the health of their coastal environments as they are susceptible to health impacts from anthropogenic inputs through both direct exposure and food web magnification. Remote biopsy samples have been widely used to reveal contaminant burdens in free-ranging bottlenose dolphins, but do not address the health consequences of this exposure. To gain insight into whether remote biopsies can also identify health impacts associated with contaminant burdens, we employed RNA sequencing (RNA-seq) to interrogate the transcriptomes of remote skin biopsies from 116 bottlenose dolphins from the northern Gulf of Mexico and southeastern U.S. Atlantic coasts. Gene expression was analyzed using principal component analysis, differential expression testing, and gene co-expression networks, and the results correlated to season, location, and contaminant burden. Season had a significant impact, with over 60% of genes differentially expressed between spring/summer and winter months. Geographic location exhibited lesser effects on the transcriptome, with 23.5% of genes differentially expressed between the northern Gulf of Mexico and the southeastern U.S. Atlantic locations. Despite a large overlap between the seasonal and geographical gene sets, the pathways altered in the observed gene expression profiles were somewhat distinct. Co-regulated gene modules and differential expression analysis both identified epidermal development and cellular architecture pathways to be expressed at lower levels in animals from the northern Gulf of Mexico. Although contaminant burdens measured were not significantly different between regions, some correlation with contaminant loads in individuals was observed among co-expressed gene modules, but these did not include classical detoxification pathways. Instead, this study identified other, possibly downstream pathways, including those involved in cellular architecture, immune response, and oxidative stress, that may prove to be contaminant responsive markers in bottlenose dolphin skin.

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A number of studies were initiated in response to the Deepwater Horizon (DWH) oil spill to understand potential injuries to bottlenose dolphins (Tursiops truncatus) that inhabit the northern Gulf of Mexico (NGoM) estuarine waters. As part of these studies, remote biopsy skin and blubber samples were collected from dolphins at six field sites that received varying degrees of oiling: Barataria Bay (BB), Chandeleur Sound West (CSW), Chandeleur Sound East (CSE), Mississippi Sound South (MSS), Mississippi Sound North (MSN), and St. Joseph Bay (SJ). Blubber samples from 108 male dolphins were analyzed for persistent organic pollutant (POP) concentrations, as high levels of POPs have been previously reported in other southeastern U.S. dolphins and the potential contribution of these compounds to adverse health effects in NGoM dolphins must be considered. Dolphin blubber levels of summed POPs (ΣPOPs) did not differ significantly across sites (F-test, P=0.9119) [µg/g lipid; geometric mean and 95% CI]; CSW [65.9 (51.4-84.6)], SJ [74.1 (53.0-104)], MSN [74.3 (58.7-93.9)], BB [75.3 (56.4-101)], CSE [80.5 (57.8-112)], and MSS [82.5 (65.9-103)]. Overall, POP concentrations were in the lower half of the range compared to previously reported concentrations from other southeastern U.S. sites. Increased dolphin mortalities have been ongoing in the NGoM and have been suggested to be linked with the DWH oil spill. In addition, lung disease, impaired adrenal function, and serum biochemical abnormalities have been reported in dolphins from BB, an area that was heavily oiled. The results of this study suggest that POPs are likely not a primary contributor to the poor health conditions and increased mortality observed in some populations of NGoM dolphins following the DWH oil spill.

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A ecologia do golfinho-nariz-de-garrafa (Tursiops truncatus) é pouco documentada no Brasil. O objetivo deste trabalho é fornecer dados sobre a distribuição, tamanho de grupo e comportamento de T. truncatus a partir de avistagens oportunistas realizadas nas adjacências da Ilha de Santa Catarina, sul do Brasil. Em cada observação de grupos da espécie foram registrados: localidade, data e hora, tamanho de grupo e comportamento. Foram observados 71 grupos entre 1989 e 2005, em todos os meses do ano. A espécie foi observada em toda região costeira da Ilha de Santa Catarina, utilizando uma grande variedade de hábitats. O tamanho de grupo variou entre 1 e 200 indivíduos, com uma média de aproximadamente 8 indivíduos (moda = 2). Interações com pelo menos três espécies de aves marinhas também foram observadas, além de outros comportamentos. Os dados obtidos sugerem que a espécie é comum ao longo da costa da Ilha de Santa Catarina, possui uma grande plasticidade no uso de hábitats, além de um conjunto variado de comportamentos.

The ecology of the bottlenose dolphin (Tursiops truncatus) is scarcely documented in Brazil. The objective of this article is to present information about the distribution, group size and behavior of T. truncatus collected oportunistically around the Island of Santa Catarina. Locality, date and time, group size and behavior were registered after each opportunistic sighting. Seventy-one groups were observed between 1989 and 2005, in all months of the year. The species was sighted along all the coast of the island, using a great variety of habitats including protected bays and exposed beaches. Group size varied from 1 to 200 individuals, with a mean group size of approximately 8 individuals (mode = 2). Interactions with three species of sea birds were also documented. Data presented here suggest that the species is common around the Island of Santa Catarina, has a great plasticity in habitat use, and a varied behavior repertoire.

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