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Biomarkers of cancer in sera of domestic dogs were detected through Raman spectroscopy with 830 nm excitation. Raman spectra of sera from 61 dogs (31 healthy and 30 with cancer, resulting in 154 and 200 spectra, respectively) were submitted to principal component analysis (PCA) for feature extraction and partial least squares (PLS) regression for discrimination between Healthy and Cancer groups. In the PCA, the peaks at 1132, 1342, 1368, and 1453 cm-1 (albumin and phenylalanine) were higher for the Cancer group. The "redshift" of the peaks at 621, 1003, and 1032 cm-1 (conformational change in proteins and/or bonds at sites close to the aromatic ring of amino acids) occurred in the Cancer group, and the peaks at 451 cm-1 (tryptophan) and 1441 cm-1 (lipids) were higher for the Healthy group. The PLS-DA classified the serum spectra in Healthy and Cancer groups with high accuracy (78%).
Assuntos
Neoplasias , Soro , Cães , Animais , Análise Discriminante , Análise Espectral Raman/métodos , Análise de Componente Principal , Biomarcadores , Neoplasias/diagnósticoRESUMO
Artificial light, as one of the environmental factors, plays a significant role in regulating the synthesis and secretion of hormones related to the coordination of parameters of life, growth, immunity, and reproductive functions of hens. The article aims to study the influence of monochrome light with different wavelengths on the biochemical parameters of hens` blood serum. Four groups of "Hy-Line W-36" crossbred hens were formed. Hens of the 1st group were kept using monochrome light with different wavelength lamps with a wavelength of ~ 460 nm, the 2nd group ~ 600 nm, the 3rd group ~ 630 nm, and the 4th group ~ 650 nm. It was found that the use of light with different wavelengths for keeping hens in cages of multilevel batteries affects hen' biochemical parameters, according to the research results. It was established that when using light with a wavelength of ~ 630 and ~ 650 nm, the indicators of clinical biochemistry of hens' blood serum were within the normal physiological values. Whereas, with the use of light with a wavelength of ~ 600 nm, an increase in the level of glucose, creatinine, total protein, total bilirubin, and phosphorus, a decrease in the ratio of calcium to phosphorus, in the activity of alkaline phosphatase, aspartate aminotransferase, and lactate dehydrogenase, were observed in the hens' blood serum. The use of light with a wavelength of ~ 460 nm was accompanied by a further increase in the level of glucose, creatinine, total protein, urea, total bilirubin, phosphorus, the activity of alkaline phosphatase, aspartate aminotransferase, lactate dehydrogenase, and gamma-glutamyltransferase, a decrease in the ratio of calcium and phosphorus.
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Animais , Bioquímica , Galinhas/sangue , Luz/efeitos adversosRESUMO
Paracoccidioidomycosis (PCM) is a systemic mycosis with high incidence in Latin America, caused by species of the genus Paracoccidioides spp. Brazil is considered to be the endemic center of this disease, which is identified as the eighth cause of mortality from chronic infectious disease in the country. There are several specific diagnostic methods in PCM, such as microbiological, immunological, histopathological, and molecular. However, the standard laboratory diagnosis depends mostly on fungus direct observation - the gold standard of PCM diagnosis. The implementation of new technologies, such as Fourier Transform Infrared (FTIR), can contribute to the clinical diagnosis trial of this disease. Here, we evaluated a new strategy for the diagnosis of PCM by using blood serum FTIR spectra from 20 patients with PCM and 20 healthy individuals. Machine learning algorithms were able to provide an overall accuracy of 91.67% by using Cubic SVM in the PCA data from FTIR results.
Assuntos
Paracoccidioides , Paracoccidioidomicose , Fotoquimioterapia , Brasil/epidemiologia , Humanos , Análise Multivariada , Paracoccidioidomicose/diagnóstico , Paracoccidioidomicose/tratamento farmacológico , Fotoquimioterapia/métodos , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
Regardless of the promising use of nanoparticles (NPs) in biomedical applications, several toxic effects have increased the concerns about the safety of these nanomaterials. Although the pathways for NPs toxicity are diverse and dependent upon many parameters such as the nature of the nanoparticle and the biochemical environment, numerous studies have provided evidence that direct contact between NPs and biomolecules or cell membranes leads to cell inactivation or damage and may be a primary mechanism for cytotoxicity. In such a context, this work focused on developing a fast and accurate method to characterize the interaction between NPs, proteins and lipidic membranes by surface plasmon resonance imaging (SPRi) technique. The interaction of gold NPs with mimetic membranes was evaluated by monitoring the variation of reflectivity after several consecutive gold NPs injections on the lipidic membranes prepared on the SPRi biochip. The interaction on the membranes with varied lipidic composition was compared regarding the total surface concentration density of gold NPs adsorbed on them. Then, the interaction of gold and silver NPs with blood proteins was analyzed regarding their kinetic profile of the association/dissociation and dissociation constants (koff). The surface concentration density on the membrane composed of 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine and cholesterol (POPC/cholesterol) was 2.5 times higher than the value found after the injections of gold NPs on POPC only or with dimethyldioctadecylammonium (POPC/DDAB). Regarding the proteins, gold NPs showed preferential binding to fibrinogen resulting in a value of the variation of reflectivity that was 8 times higher than the value found for the other proteins. Differently, silver NPs showed similar interaction on all the tested proteins but with a variation of reflectivity on immunoglobulin G (IgG) 2 times higher than the value found for the other tested proteins.
Assuntos
Nanopartículas Metálicas , Nanopartículas , Ressonância de Plasmônio de Superfície/métodos , Prata/química , Proteínas/química , Nanopartículas/química , Ouro/química , Nanopartículas Metálicas/químicaRESUMO
Frontotemporal lobar degeneration describes a group of progressive brain disorders that primarily are associated with atrophy of the prefrontal and anterior temporal lobes. Frontotemporal lobar degeneration is considered to be equivalent to frontotemporal dementia. Frontotemporal dementia is characterized by progressive impairments in behavior, executive function, and language. There are two main clinical subtypes: behavioral-variant frontotemporal dementia and primary progressive aphasia. The early diagnosis of frontotemporal dementia is critical for developing management strategies and interventions for these patients. Without validated biomarkers, the clinical diagnosis depends on recognizing all the core or necessary neuropsychiatric features, but misdiagnosis often occurs due to overlap with a range of neurologic and psychiatric disorders. In the studies reviewed a very large number of microRNAs were found to be dysregulated but with limited overlap between individual studies. Measurement of specific miRNAs singly or in combination, or as miRNA pairs (as a ratio) in blood plasma, serum, or cerebrospinal fluid enabled frontotemporal dementia to be discriminated from healthy controls, Alzheimer's disease, and amyotrophic lateral sclerosis. Furthermore, upregulation of miR-223-3p and downregulation of miR-15a-5p, which occurred both in blood serum and cerebrospinal fluid, distinguished behavioral-variant frontotemporal dementia from healthy controls. Downregulation of miR-132-3p in frontal and temporal cortical tissue distinguished frontotemporal lobar degeneration and frontotemporal dementia, respectively, from healthy controls. Possible strong miRNA biofluid biomarker contenders for behavioral-variant frontotemporal dementia are miR-223-3p, miR-15a-5p, miR-22-3p in blood serum and cerebrospinal fluid, and miR-124 in cerebrospinal fluid. No miRNAs were identified able to distinguish between behavioral-variant frontotemporal dementia and primary progressive aphasia subtypes. Further studies are warranted on investigating miRNA expression in biofluids and frontal/temporal cortical tissue to validate and extend these findings.
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A main cause of vision loss in the elderly is age-related macular degeneration (AMD). Among the cellular, biochemical, and molecular changes linked to this disease, inflammation and angiogenesis appear as being crucial in AMD pathogenesis and progression. There are two forms of the disease: dry AMD, accounting for 80-90% of cases, and wet AMD. The disease usually begins as dry AMD associated with retinal pigment epithelium and photoreceptor degeneration, whereas wet AMD is associated with choroidal neovascularization resulting in severe vision impairment. The new vessels are largely malformed, leading to blood and fluid leakage within the disrupted tissue, which provokes inflammation and scar formation and results in retinal damage and detachment. MicroRNAs are dysregulated in AMD and may facilitate the early detection of the disease and monitoring disease progression. Two recent reviews of microRNAs in AMD had indicated weaknesses or limitations in four earlier investigations. Studies in the last three years have shown considerable progress in overcoming some of these concerns and identifying specific microRNAs as biomarkers for AMD. Further large-scale studies are warranted using appropriate statistical methods to take into account gender and age disparity in the study populations and confounding factors such as smoking status.
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The background of this study was blood profile and cows' behavior that could be used as a tool to diagnose reproductive status in the dairy cows. This research was aimed to determine biochemical blood profile, progesterone level, and behaviors of Friesian Holstein crossbreed dairy cow in the tropical climates. Fifteen Friesian-Holstein crossbreeds cows, in early lactation, reared by local farmers were divided into two barn types: individual and groups of cows. Blood samples were collected from the jugular vein using a 22G needle on days 0, 14, and 28. For the determination of blood profile, blood was collected into Vacuntainer® blood tubes containing ethylenediaminetetraacetic acid (EDTA) to arrest glycolysis. The blood was transported to the laboratory with a coolbox. The concentration of progesterone in plasma was assayed byenzyme-linked immunosorbent assay (ELISA) using a commercial kit and the biochemical blood profile serum was measured by using microlab 300. The variables observed were glucose, cholesterol, urea, and non-pregnant progesterone. Data obtained was examined by T-test analysis. The result showed that the Temperature-Humidity Index were 81.3 and 79.6 respectively, there were no significant differences between two observation, in terms of total feeding time (3.06±0.48 and 2.91±0.22 hours/day), total water consumption (86.23±0.58 and 85.37±0.88 l/day), total standing (12.70±0.21 and 12.83±0.34 hours/day), total laying (11.30±0.71 and 11.16±0.62 hours/day). The blood profile and progesterone levels were lower than the normal range in dairy cows. Also, the result indicated that blood parameters were not significantly different (P > 0.05) between individual pen and group. The result indicated that the progesterone level was not significant in two models of management dairy cows. The conclusion of the research is the climate can affect behavior and the blood parameters. Blood profiles [...].
Assuntos
Feminino , Animais , Bovinos , Aclimatação , Análise Química do Sangue/veterinária , Comportamento Animal , Fertilidade/efeitos da radiação , Progesterona/análiseRESUMO
The background of this study was blood profile and cows' behavior that could be used as a tool to diagnose reproductive status in the dairy cows. This research was aimed to determine biochemical blood profile, progesterone level, and behaviors of Friesian Holstein crossbreed dairy cow in the tropical climates. Fifteen Friesian-Holstein crossbreeds cows, in early lactation, reared by local farmers were divided into two barn types: individual and groups of cows. Blood samples were collected from the jugular vein using a 22G needle on days 0, 14, and 28. For the determination of blood profile, blood was collected into Vacuntainer® blood tubes containing ethylenediaminetetraacetic acid (EDTA) to arrest glycolysis. The blood was transported to the laboratory with a coolbox. The concentration of progesterone in plasma was assayed byenzyme-linked immunosorbent assay (ELISA) using a commercial kit and the biochemical blood profile serum was measured by using microlab 300. The variables observed were glucose, cholesterol, urea, and non-pregnant progesterone. Data obtained was examined by T-test analysis. The result showed that the Temperature-Humidity Index were 81.3 and 79.6 respectively, there were no significant differences between two observation, in terms of total feeding time (3.06±0.48 and 2.91±0.22 hours/day), total water consumption (86.23±0.58 and 85.37±0.88 l/day), total standing (12.70±0.21 and 12.83±0.34 hours/day), total laying (11.30±0.71 and 11.16±0.62 hours/day). The blood profile and progesterone levels were lower than the normal range in dairy cows. Also, the result indicated that blood parameters were not significantly different (P > 0.05) between individual pen and group. The result indicated that the progesterone level was not significant in two models of management dairy cows. The conclusion of the research is the climate can affect behavior and the blood parameters. Blood profiles [...].(AU)
Assuntos
Animais , Feminino , Bovinos , Análise Química do Sangue/veterinária , Progesterona/análise , Comportamento Animal , Aclimatação , Fertilidade/efeitos da radiaçãoRESUMO
Multiple sclerosis is an autoimmune neurodegenerative disease of the central nervous system characterized by pronounced inflammatory infiltrates entering the brain, spinal cord and optic nerve leading to demyelination. Focal demyelination is associated with relapsing-remitting multiple sclerosis, while progressive forms of the disease show axonal degeneration and neuronal loss. The tests currently used in the clinical diagnosis and management of multiple sclerosis have limitations due to specificity and sensitivity. MicroRNAs (miRNAs) are dysregulated in many diseases and disorders including demyelinating and neuroinflammatory diseases. A review of recent studies with the experimental autoimmune encephalomyelitis animal model (mostly female mice 6-12 weeks of age) has confirmed miRNAs as biomarkers of experimental autoimmune encephalomyelitis disease and importantly at the pre-onset (asymptomatic) stage when assessed in blood plasma and urine exosomes, and spinal cord tissue. The expression of certain miRNAs was also dysregulated at the onset and peak of disease in blood plasma and urine exosomes, brain and spinal cord tissue, and at the post-peak (chronic) stage of experimental autoimmune encephalomyelitis disease in spinal cord tissue. Therapies using miRNA mimics or inhibitors were found to delay the induction and alleviate the severity of experimental autoimmune encephalomyelitis disease. Interestingly, experimental autoimmune encephalomyelitis disease severity was reduced by overexpression of miR-146a, miR-23b, miR-497, miR-26a, and miR-20b, or by suppression of miR-182, miR-181c, miR-223, miR-155, and miR-873. Further studies are warranted on determining more fully miRNA profiles in blood plasma and urine exosomes of experimental autoimmune encephalomyelitis animals since they could serve as biomarkers of asymptomatic multiple sclerosis and disease course. Additionally, studies should be performed with male mice of a similar age, and with aged male and female mice.
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Mucor circinelloides is an opportunistic human pathogen that is used to study mucormycosis, a rare but lethal infection in susceptible immunosuppressed patients. However, the virulence characteristics of this pathogen have not been fully elucidated. In this study, sporangiospores (spores) produced on YPG medium supplemented with native blood serum increased the virulence of M. circinelloides compared with spores produced on YPG supplemented with denatured blood serum or on YPG alone. The spores produced from YPG supplemented with native blood serum increased nematode death and led to significant increases in interleukin (IL)-6, IL-1ß, macrophage inhibitory protein-2, and tumour necrosis factor α mRNA levels in liver and lung tissues from infected diabetic mice compared with those in tissues from animals infected with spores produced in the presence of YPG supplemented with denatured blood serum or of YPG alone. Moreover, spores produced from cultures supplemented with native blood serum showed increased germination rates and longer hyphae compared with other spores. The spores produced in YPG supplemented with native blood serum also enhanced resistance to stress factors and H2O2 and increased thermotolerance compared with spores produced under other conditions. In addition, spores produced in presence of blood serum increased the ability of the pathogen to survive in the presence of macrophages. Taken together, our results showed that these factors were important features for fungal virulence in humans and suggested that thermolabile components in the blood serum may induce M. circinelloides virulence.
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Mucor/patogenicidade , Mucormicose/sangue , Soro/microbiologia , Esporos Fúngicos/metabolismo , Animais , Citocinas/metabolismo , Diabetes Mellitus Experimental , Humanos , Peróxido de Hidrogênio , Hifas/crescimento & desenvolvimento , Interleucina-1beta/metabolismo , Interleucina-6/metabolismo , Pulmão , Macrófagos/microbiologia , Masculino , Camundongos , RNA Mensageiro/metabolismo , Esporos Fúngicos/crescimento & desenvolvimento , VirulênciaRESUMO
Approximately 255 million people consume illicit drugs every year, among which 18 million use cocaine. A portion of this drug is represented by crack, but it is difficult to estimate the number of users since most are marginalized. However, there are no recognized efficacious pharmacotherapies for crack-cocaine dependence. Inflammation and infection in cocaine users may be due to behavior adopted in conjunction with drug-related changes in the brain. To understand the metabolic changes associated with the drug abuse disorder and identify biomarkers, we performed a 1H NMR-based metabonomic analysis of 44 crack users' and 44 healthy volunteers' blood serum. The LDA model achieved 98% of accuracy. From the water suppressed 1H NMR spectra analyses, it was observed that the relative concentration of lactate was higher in the crack group, while long chain fatty acid acylated carnitines were decreased, which was associated with their nutritional behavior. Analyses of the aromatic region of CPMG 1H NMR spectra demonstrated histidine and tyrosine levels increased in the blood serum of crack users. The reduction of carnitine and acylcarnitines and the accumulation of histidine in the serum of the crack users suggest that histamine biosynthesis is compromised. The tyrosine level points to altered dopamine concentration.
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Transtornos Relacionados ao Uso de Cocaína , Cocaína Crack/farmacologia , Espectroscopia de Ressonância Magnética/métodos , Metaboloma/efeitos dos fármacos , Coleta de Amostras Sanguíneas , Carnitina/sangue , Estudos de Casos e Controles , Histidina/sangue , Humanos , Ácido Láctico/sangue , Tirosina/sangueRESUMO
Nuclear magnetic resonance (NMR) spectroscopy in combination with chemometrics can be applied in the analysis of complex biological samples in many ways. For example, we can analyze lipids, elucidate their structures, determine their nutritional values, and determine their distribution in blood serum. As lipids are not soluble in water, they are transported in blood as lipid-rich self-assembled particles, divided into different density assemblies from high- to very-low-density lipoproteins (HDL to VLDL), or by combining with serum proteins, such as albumins (human serum albumins (HSA)). Therefore, serum lipids can be analyzed as they are using only a 1:1 (v/v) dilution with a buffer or deuterated water prior to analysis by applying 1H NMR or 1H NMR edited-by-diffusion techniques. Alternatively, lipids can be extracted from the serum using liquid partition equilibrium and then analyzed using liquid-state NMR techniques. Our chapter describes protocols that are used for extraction of blood serum lipids and their quantitative 1H NMR (1H qNMR) analysis in lipid extracts as well as 1H NMR edited by diffusion for direct blood serum lipid analysis.
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Lipídeos/sangue , Espectroscopia de Ressonância Magnética , Metabolômica , Biomarcadores , Humanos , Espectroscopia de Ressonância Magnética/métodos , Metabolômica/métodosRESUMO
The present study aimed at investigating possible alterations in the serum lipid profile of euthymic patients with bipolar disorder type I (BD) compared to healthy controls (HC). Thirty-five individuals from both genders were recruited, with 14 diagnosed and treated as BD patients (BD group) and 21 healthy subjects (HC group). Clinical assessment was based on the Structured Clinical Interview for DSM-IV Axis I Disorders (SCID-I), Young Mania Rating Scale (YMRS), and 17-items of Hamilton Depression Rating Scale (HDRS-17) data, which were used to confirm diagnosis, to verify psychiatric comorbidities, and to estimate the severity of manic and depressive symptoms. Ultra-high performance liquid chromatography (UHPLC) coupled to high resolution mass spectrometry (HRMS) was applied to analyze the lipids extracted from all serum samples from both studied groups. In this pioneer and exploratory study, we observed different serum lipid profiles for BD and HC groups, especially regarding glycerophospholipid, glycerolipid, and sphingolipid distribution. Multivariate statistical analyses indicated that 121 lipids were significantly different between BD and HC. Phosphatidylinositols were identified as the most altered lipids in BD patient sera. The results of this preliminary study reinforce the role of lipid abnormalities in BD and offer additional methodological possibilities for investigation in the field.
Assuntos
Transtorno Bipolar/sangue , Lipídeos/sangue , Espectrometria de Massas , Adulto , Transtorno Bipolar/diagnóstico , Transtorno Bipolar/psicologia , Estudos de Casos e Controles , Comorbidade , Depressão/sangue , Depressão/diagnóstico , Depressão/psicologia , Manual Diagnóstico e Estatístico de Transtornos Mentais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fosfatidilinositóis/sangueRESUMO
ABSTRACT Objective. To determine Trypanosoma cruzi (T. cruzi), Leishmania mexicana (L.mexicana) and Leishmania braziliensis (L.braziliensis) circulating antibodies in dogs from Chontalpa region in Tabasco, Mexico using ELISA diagnostic techniques Fe-SOD and Western blot. Materials and methods. For this study, 119 serums were obtained from domiciled dogs. Serums were tested for antibodies against T. cruzi, L. mexicana and L. braziliensis, using ELISA and Western Blot sod as diagnostic test. The antigenic fraction used in both tests was the Fe-SOD excreted by the species of Leishmania and Trypanosoma. Results. The obtained frequency in this study was 3.36% for T. cruzi, 9.24% for L. mexicana and 10.08% for L. braziliensis. Conclusions. The present study has demonstrated the presence of antibodies to these parasites in Chontalpa region from Tabasco, Mexico.
RESUMEN Objetivo. Determinar la frecuencia de anticuerpos circulantes de Trypanosoma cruzi (T. cruzi), Leishmania mexicana (L. mexicana) y Leishmania braziliensis (L. braziliensis) en una población de perros usando ELISA Fe-SOD y Western blot en la región Chontalpa del estado de Tabasco, México. Materiales y métodos. Para este estudio se obtuvieron 119 sueros de perros domiciliados, con el consentimiento previo de los propietarios. Los sueros fueron analizados para detectar anticuerpos contra T. cruzi, L. mexicana, y L. braziliensis, usando como prueba diagnóstica ELISA-sod y Western Blot. La fracción antigénica utilizada en las dos pruebas fue la Fe-SOD excretada por las especies de Trypanosoma y Leishmania. Resultados. La frecuencia obtenida en este estudio fue de 3.36% para T. cruzi, 9.24% para L. mexicana y 10.08% L. braziliensis. Conclusiones. El presente estudio demostró la presencia de anticuerpos para estos parásitos en la región Chontalpa del estado de Tabasco, México.
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Cancer is responsible for millions of deaths worldwide, but most base diseases may be cured if detected early. Screening tests may be used to identify early-stage malignant neoplasms. However, the major screening tool for prostate cancer, the prostate-specific antigen test, has unsuitable sensitivity. Since cancer cells may affect the pattern of consumption and excretion of nucleosides, such biomolecules are putative biomarkers that can be used for diagnosis and treatment evaluation. Using a previously validated method for the analysis of nucleosides in blood serum by capillary electrophoresis with UV-vis spectroscopy detection, we investigated 60 samples from healthy individuals and 42 samples from prostate cancer patients. The concentrations of nucleosides in both groups were compared and a multivariate partial least squares-discriminant analysis classification model was optimized for prediction of prostate cancer. The validation of the model with an independent sample set resulted in the correct classification of 82.4% of the samples, with sensitivity of 90.5% and specificity of 76.7%. A significant downregulation of 5-methyluridine and inosine was observed, which can be indicative of the carcinogenic process. Therefore, such analytes are potential candidates for prostate cancer screening. Graphical Abstract Separation of the studied nucleosides and the internal standard 8-Bromoguanosine by CE-UV (a); classification of the external validation samples (30 from healthy volunteers and 21 from prostate cancer patients) by the developed Partial Least Square - Discriminant Analysis (PLS-DA) model with accuracy of 82.4% (b); Receiver Operating Characteristics (ROC) curve (c); and Variable Importance in the Projection (VIP) values for the studied nucleosides (d). A significant down-regulation of 5- methyluridine (5mU) and inosine (I) was observed, which can be indicative of the presence of prostate tumors.
Assuntos
Eletroforese Capilar/métodos , Nucleosídeos/sangue , Neoplasias da Próstata/diagnóstico , Espectrofotometria Ultravioleta/métodos , Biomarcadores Tumorais , Humanos , Masculino , Estrutura Molecular , Nucleosídeos/química , Nucleosídeos/metabolismoRESUMO
El calcio es uno de los minerales que interviene en diversas funciones celulares, así como también en la estructura ósea, coagulación y señales intracelulares. En el presente trabajo se determinó la concentración de calcio en calostro y suero sanguíneo de madres lactantes que asistieron a la Maternidad Dr. José María Vargas. Valencia, Edo. Carabobo. Muestra conformada por 50 mujeres lactantes, edad 18-35 años. Se realizó: evaluación clínica con elaboración de historia médica; recolección de calostro por extracción manual estandarizada según la Organización Mundial de la Salud. Se les extrajo muestra de sangre periférica (10 ml), mediante punción venosa Se determinó concentración de calcio en calostro y suero sanguíneo por Espectrofotometría de Absorción Atómica bajo la modalidad de llama, equipo Perkin Elmer modelo 3100. Edad promedio 24.5±3.2 años; concentración de calcio en calostro 209,98 a 251,54 µg/mL sin asociación significativa entre la edad y los valores de calcio agrupados en percentiles. La concentración de calcio en suero sanguíneo predominó en el percentil 25 con valores ≤ 1,414 mmol/L; concentraciones de calcio en calostro de ≥ 251,55 µg/mL; la mayoría de las madres lactantes estaban ubicadas en los estratos III, IV y V. Se concluye, que las concentraciones promedio de calcio en muestras de calostro y suero sanguíneo de la población en estudio fueron normales en el límite superior para calostro y en el límite inferior en suero sanguíneo(AU)
Calcium is one of the minerals involved in various cellular operations, as well as in bone structure, coagulation and intracellular signals. In this paper the concentration of calcium in blood serum and colostrum of nursing mothers who attended the Maternity Dr. José Maria Vargas. Valencia, Edo. Carabobo was determined. The sample consisted of 50 lactating women, aged 18-35 years. We performed: clinical evaluation development of medical history. Colostrum collection by manual removal standardized by WHO. It was took peripheral blood sample (10 ml) by venipuncture; calcium concentration was determined in colostrum and blood serum by atomic absorption spectrophotometry in the form of flame, using Perkin Elmer model 3100. Results: The mean age of 24.5 ± 32 years; calcium concentration in colostrum from 209.98 to 251.54 mg / mL without significant association between age and calcium values grouped into percentiles. The concentration of calcium in blood serum predominated in the 25th percentile with values ≤ 1.414mmol /L; calcium concentrations in colostrum ≥ 251.55 mg / mL; most nursing mothers were located in strata III, IV and V. It is concluded that the average calcium concentrations in colostrum samples and blood serum of the study population were normal in the upper limit for colostrum, and the lower limit in blood serum, with significant association to socioeconomic status(AU)
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Humanos , Feminino , Gravidez , Recém-Nascido , Lactente , Adulto , Aleitamento Materno , Cálcio , Colostro , Substitutos do Leite Humano , Nutrição do Lactente , Espectrofotometria , Recém-Nascido , Gravidez , NutrientesRESUMO
Brucellosis is a zoonotic disease with common agent is Brucella abortus in bovine specie. The disease in bulls is usually asymptomatic and could have absence of anti-B. abortus antibody in serum. It was investigated of the occurrence of bovine brucellosis in bulls, which involved testing blood serum and seminal plasma from 177 bulls used for natural mating or as semen donors. Blood was collected from all the animals by venipuncture of the jugular vein to obtain the serum samples, while seminal plasma was obtained from the animals by electroejaculation. Samples were tested in a screening test (rose Bengal test). In the second stage, the samples testing positive in the RBT were subjected to the confirmatory test (2-mercaptoethanol). None of the animals tested positive when blood serum was used. However, 5.06% of the bulls were considered reagent positive when seminal plasma was tested. It also sought to determine whether there was an association between the kind of production and the destination of animals with positive results. The calculated odds ratio indicated that bulls of the kind of production dairy/both (beef and dairy) had a higher risk of brucellosis infection. It is recommended that seminal plasma agglutination and seroagglutination techniques are applied concomitantly.(AU)
Brucelose é uma zoonose que nos bovinos tem como principal agente a bactéria Brucella abortus. A doença nos touros é geralmente assintomática e pode haver ausência de anticorpos anti- B. abortus no soro sanguíneo. O presente estudo teve como objetivo principal avaliar a ocorrência da brucelose bovina em reprodutores para isso foram empregados testes oficiais tendo como material o soro sanguíneo e o plasma seminal. Foram utilizados 177 machos bovinos destinados à monta natural ou doação de sêmen. O soro sanguíneo foi obtido através de venopunção da jugular e o plasma seminal através da eletroejaculação. As amostras foram submetidas ao teste de triagem (Antígeno acidificado tamponado) e na segunda etapa, apenas as amostras positivas na triagem foram submetidas ao exame confirmatório (2-mercaptoetanol). Não houveram animais positivos quando utilizado o soro sanguíneo. Para plasma seminal houve 5,06% de touros reagentes. Avaliou-se também se sistema produtivo ou a destinação do animal possuíam associação com resultados positivos. O cálculo de odds ratio demonstrou que os reprodutores bovinos do sistema produtivo misto/leite tem maior risco de infecção para brucelose. Recomenda-se que a técnica de sêmen plasma aglutinação seja feita em conjunto com a soroaglutinação.(AU)
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Animais , Bovinos , Brucelose Bovina/diagnóstico , Testes Hematológicos/métodos , Testes Sorológicos , SêmenRESUMO
The development and validation of methodologies for the analysis of biological samples is of outcome importance in order to obtain trustworthy results. This work reports a novel CE-UV method for the assessment of nucleosides, putative tumor biomarkers, in blood serum. The separation of seven nucleosides within c.a. 20 min has been achieved with: BGE 30 mmol/L borate at pH 9.90, 50 mmol/L CTAB, and 10% methanol; V = -10 kV; T = 20°C; and capillary dimensions of 56 cm × 50 µm. The sample plug was concentrated by a modified large volume sample stacking strategy that provided better detectability. Validation showed that the method is suitable for bioanalytical purposes and initial applications in serum samples from healthy subjects are also presented. Finally, statistical methods were applied to verify the effect of characteristics such as age, smoking habits, and alcohol consumption on nucleoside concentrations in blood serum. Univariate statistical analysis tests emphasized the need for age matching, which was confirmed by PCA-DA and PLS-DA. Cancer history in the nearby family may also interfere in nucleoside levels in blood serum, since adenosine concentrations were statistically higher for volunteers who declared having diseased relatives.
Assuntos
Biomarcadores Tumorais/sangue , Eletroforese Capilar/métodos , Nucleosídeos/sangue , Adulto , Humanos , Limite de Detecção , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Reprodutibilidade dos Testes , Raios Ultravioleta , Adulto JovemRESUMO
AbstractObjective The objective of this study was to clarify significant risk factors for postoperative complications in the oral cavity in patients who underwent oral surgery, excluding those with oral cancer.Material and Methods This study reviewed the records of 324 patients who underwent mildly to moderately invasive oral surgery (e.g., impacted tooth extraction, cyst excision, fixation of mandibular and maxillary fractures, osteotomy, resection of a benign tumor, sinus lifting, bone grafting, removal of a sialolith, among others) under general anesthesia or intravenous sedation from 2012 to 2014 at the Department of Oral and Maxillofacial Reconstructive Surgery, Hiroshima University Hospital.Results Univariate analysis showed a statistical relationship between postoperative complications (i.e., surgical site infection, anastomotic leak) and diabetes (p=0.033), preoperative serum albumin level (p=0.009), and operation duration (p=0.0093). Furthermore, preoperative serum albumin level (<4.0 g/dL) and operation time (≥120 minutes) were found to be independent factors affecting postoperative complications in multiple logistic regression analysis results (odds ratio 3.82, p=0.0074; odds ratio 2.83, p=0.0086, respectively).Conclusion Our results indicate that a low level of albumin in serum and prolonged operation duration are important risk factors for postoperative complications occurring in the oral cavity following oral surgery.
Assuntos
Humanos , Masculino , Feminino , Pré-Escolar , Criança , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Adulto Jovem , Doenças da Boca/etiologia , Procedimentos Cirúrgicos Bucais/efeitos adversos , Complicações Pós-Operatórias/etiologia , Fatores Etários , Perda Sanguínea Cirúrgica , Complicações do Diabetes , Duração da Cirurgia , Análise de Regressão , Estudos Retrospectivos , Fatores de Risco , Albumina Sérica/análise , Fatores SexuaisRESUMO
Brucellosis is a zoonotic disease with common agent is Brucella abortus in bovine specie. The disease in bulls is usually asymptomatic and could have absence of anti-B. abortus antibody in serum. It was investigated of the occurrence of bovine brucellosis in bulls, which involved testing blood serum and seminal plasma from 177 bulls used for natural mating or as semen donors. Blood was collected from all the animals by venipuncture of the jugular vein to obtain the serum samples, while seminal plasma was obtained from the animals by electroejaculation. Samples were tested in a screening test (rose Bengal test). In the second stage, the samples testing positive in the RBT were subjected to the confirmatory test (2-mercaptoethanol). None of the animals tested positive when blood serum was used. However, 5.06% of the bulls were considered reagent positive when seminal plasma was tested. It also sought to determine whether there was an association between the kind of production and the destination of animals with positive results. The calculated odds ratio indicated that bulls of the kind of production dairy/both (beef and dairy) had a higher risk of brucellosis infection. It is recommended that seminal plasma agglutination and seroagglutination techniques are applied concomitantly.
Brucelose é uma zoonose que nos bovinos tem como principal agente a bactéria Brucella abortus. A doença nos touros é geralmente assintomática e pode haver ausência de anticorpos anti- B. abortus no soro sanguíneo. O presente estudo teve como objetivo principal avaliar a ocorrência da brucelose bovina em reprodutores para isso foram empregados testes oficiais tendo como material o soro sanguíneo e o plasma seminal. Foram utilizados 177 machos bovinos destinados à monta natural ou doação de sêmen. O soro sanguíneo foi obtido através de venopunção da jugular e o plasma seminal através da eletroejaculação. As amostras foram submetidas ao teste de triagem (Antígeno acidificado tamponado) e na segunda etapa, apenas as amostras positivas na triagem foram submetidas ao exame confirmatório (2-mercaptoetanol). Não houveram animais positivos quando utilizado o soro sanguíneo. Para plasma seminal houve 5,06% de touros reagentes. Avaliou-se também se sistema produtivo ou a destinação do animal possuíam associação com resultados positivos. O cálculo de odds ratio demonstrou que os reprodutores bovinos do sistema produtivo misto/leite tem maior risco de infecção para brucelose. Recomenda-se que a técnica de sêmen plasma aglutinação seja feita em conjunto com a soroaglutinação.