RESUMO

Normal reference intervals (RI) of hematologic and biochemical parameters are important for assessing and monitoring the health status of captive and free-living chelonians; however, such information is not available for most wildlife species. Giant Galapagos tortoises are one of the most iconic animals on earth and health information can make an important contribution to their conservation and management. This study provides formal RI of haematology and plasma biochemistry parameters and describes cell morphology along with morphometrics of free-living Eastern Santa Cruz (Chelonoidis donfaustoi), Española (Chelonoidis hoodensis) and San Cristóbal tortoises (Chelonoidis chathamensis). We explored differences in blood parameters between sexes, across the tortoise species in this study and with previously published RI of the Western Santa Cruz tortoise (Chelonoidis porteri). Biochemistry parameters of both Santa Cruz species were overall more similar to each other than to Española and San Cristobal tortoises. This research constitutes the first RI for these three Galapagos tortoise species and may be of value for advising captive-breeding and conservation plans. We recommend further research to establish RI in additional tortoise species so we may better understand and interpret haematology and biochemistry parameters as a valuable conservation tool for species of this critically endangered taxon.

RESUMO

This study aimed to determine the impact of age on the fecal microbiota in the genetic nucleus of cattle, with a focus on microbial richness, composition, functional diversity, and correlations with blood parameters. Fecal and blood samples from 21 cattle were analyzed using 16S rRNA gene sequencing. Older cattle exhibited greater bacterial diversity and abundance, with significant changes in alpha diversity indices (p < 0.05). Beta diversity analysis revealed significant variations in microbial composition between age groups and the interaction of age and sex (p < 0.05). Correlations between alpha diversity, community composition, and hematological values highlighted the influence of microbiota on bovine health. Beneficial butyrate-producing bacteria, such as Ruminococcaceae, were more abundant in older cattle, suggesting a role in gut health. Functional diversity analysis indicated that younger cattle had significantly more abundant metabolic pathways in fermentation and anaerobic chemoheterotrophy. These findings suggest management strategies including tailored probiotic therapies, dietary adjustments, and targeted health monitoring to enhance livestock health and performance. Further research should include comprehensive metabolic analyses to better correlate microbiota changes with age-related variations, enhancing understanding of the complex interactions between microbiota, age, and reproductive status.

RESUMO

The objective of this study was to evaluate the effect of different nutritional strategies on the intensification of beef cattle farming on pastures during the dry period of the year. Eighty male cattle (testers) were randomly allocated to 16 paddocks formed with Mombaça grass (Megathyrsus maximus), totaling five animals (testers) per paddock. The strategies consisted of two LCs [10 and 16.7 g·kg-1 body weight (BW)] and two PSs with DDGS and SBM in a completely randomized design with a 2 × 2 factorial arrangement. The chemical, structural, and productive characteristics of the forage were evaluated, as well as the performance, productivity, and serum parameters of the supplemented animals. The forage presented a greater L:C (p = 0.033) and CP content (p = 0.007) when the lowest LC was used. Animals that received the highest LC had the highest supplement intake (p < 0.001) and the lowest pasture intake (p < 0.001). The nutritional strategy with an LC of 16.7 g·kg-1 of body weight (BW) resulted in a greater increase in total BW, i.e., 200 kg·BW ha-1 more. Therefore, higher levels of concentrate ensure greater productivity for beef cattle grazing, and DDGS can replace SBM in supplements used in the intensive raising of beef cattle on pasture without compromising the performance and productivity of the animals.

RESUMO

Our study aimed to establish reference values for nesting females and compare them with those previously reported to understand olive ridley turtles' health status and contribute to long-term health assessment and monitoring in foraging and nesting areas from the state of Sinaloa, Mexico. In August and September 2018, morphometric data and biochemical profiles were collected from 33 nesting olive ridley turtles from Ceuta Beach Sanctuary (CBS) and 14 foraging female turtles captured at the foraging site, Navachiste Marine Area (NMA). Nesting turtles sampled had greater CCL (65.86 ± 1.70 cm) than those from the foraging area (61.54 ± 1.22) (p < 0.05). Regarding biochemical profiles, post-nesting turtles had higher packed cell volume (PCV), albumin, blood urea nitrogen (BUN), cholesterol, triglycerides, and calcium than turtles from the foraging area (p < 0.05). Phosphorus levels were higher in foraging turtles than in nesting turtles (p = 0.001), while the remaining parameters showed no significant differences. The present study describes for the first time the blood biochemical values of nesting turtles from the Ceuta Beach Sanctuary in southern Sinaloa, Mexico, similar to those of foraging turtles from the north of the state. The significant differences observed between the two analysis groups may be due to the energy reserves and reproductive and nesting activity of the nesting turtles, so the blood biochemistry values described in this study can be used as a standard reference blood value for the olive ridley turtle population of Sinaloa, Mexico.

Assuntos

Comportamento de Nidação , Tartarugas , Animais , Tartarugas/sangue , Tartarugas/fisiologia , México , Feminino , Monitoramento Ambiental , Nitrogênio da Ureia Sanguínea , Valores de Referência , Hematócrito

RESUMO

This study investigated the use of infrared thermography (IRT) to identify the dark, firm, and dry (DFD) phenomenon in Brazilian beef, which is a significant concern for the industry because of its inferior quality and reduced shelf life. This study examined 113 Nellore bulls and analyzed their minimum and maximum ocular temperatures using IRT. The results highlight the efficacy of thermal images (IRTmax) as a significant predictor, with R2 values ranging from 0.84 to 0.88 for calibration models. The inclusion of parameters such as glucose and lactate further enhanced prediction accuracy. The models also revealed that the combination of features, such as lightness (L*), redness (a*), and yellowness (b*), contributed to the precise prediction of pHu, with an R2 of 0.88. In model validation, RMSEP ranged from 0.104 to 0.158, indicating good generalization capability. The RPD, ranging from 1.7 to 2.6, suggests satisfactory quantitative prediction. The statistical significance of all models, evidenced by P-values <0.001, strengthens the reliability of the results. In conclusion, the models support the use of IRT as a tool for identifying pHu alterations in carcasses. When combined with blood parameters, they may exhibit even greater efficiency in predicting pHu in Nelore cattle carcasses, highlighting the potential applicability of these methods in the beef industry.

Assuntos

Carne Vermelha , Termografia , Animais , Bovinos , Termografia/veterinária , Termografia/métodos , Concentração de Íons de Hidrogênio , Carne Vermelha/análise , Masculino , Cor , Raios Infravermelhos , Qualidade dos Alimentos , Reprodutibilidade dos Testes , Brasil

RESUMO

The aim of study was to determine the relationship between metabolic parameters, body weight and body growth of piglets at birth and weaning. The experiment included 80 piglets obtained from F1 generation sows. Body weight was measured at birth (BW0), 24h (BW1) after birth and on day 25 (at weaning, BW2). Blood sampling was performed at the beginning of life (3rd day after birth) and at weaning (21st day after birth). BW0 and BW1 positively correlated with cholesterol and negatively with urea values at the beginning of life and RBC values at weaning. BW2 positively correlates with albumin and cortisol values at the beginning of life, total proteins, and globulins at weaning, and negatively correlates with erythrocyte values at weaning. Piglet growth from birth to weaning (BWG2-0) correlates positively with total proteins, albumin, and cortisol at the beginning of life and total proteins at weaning. ROC analysis shows that MCHC, TPROT, GLOB, CHOL and AST at the beginning of life can distinguish fast-growing from slow-growing piglets from birth to weaning period. The use of blood parameters enables early recognition of growth rate in piglets, which can help to optimize all further steps to achieve the best possible growth.

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O objetivo do estudo foi determinar a relação entre parâmetros metabólicos, peso corporal e crescimento corporal de leitões ao nascimento e desmame. A experiência incluiu 80 leitões obtidos de porcas da geração F1. O peso corporal foi medido ao nascimento (BW0), 24h (BW1) após o nascimento e no dia 25 (no desmame, BW2). A amostragem de sangue foi realizada no início da vida (3º dia após o nascimento) e no desmame (21º dia após o nascimento). BW0 e BW1 correlacionaram-se positivamente com o colesterol e negativamente com os valores de uréia no início da vida e com os valores de hemácias no desmame. BW2 correlaciona-se positivamente com os valores de albumina e cortisol no início da vida, proteínas totais e globulinas no desmame, e negativamente com os valores de eritrócitos no desmame. O crescimento do leitão desde o nascimento até o desmame (BWG2-0) se correlaciona positivamente com as proteínas totais, albumina e cortisol no início da vida e com as proteínas totais no desmame. A análise ROC mostra que MCHC, TPROT, GLOB, CHOL e AST no início da vida pode distinguir leitões de crescimento rápido de leitões de crescimento lento desde o nascimento até o desmame. O uso de parâmetros sanguíneos permite o reconhecimento precoce da taxa de crescimento em leitões, o que pode ajudar a otimizar todas as etapas posteriores para alcançar o melhor crescimento possível.

Assuntos

Animais , Suínos/crescimento & desenvolvimento , Suínos/sangue , Peso Corporal , Aumento de Peso

RESUMO

This study was conducted to evaluate the effects of different dietary amounts of Moringa oleifera meal (MOM), consisting of leaves and small edible green stems, on growth performance, carcass traits, and blood parameters of finisher male layer-type slow-growing chickens raised with outdoor access. A total of 198 Dominant Blue D 107, 72-day-old male chickens were randomly assigned to tree dietary treatments containing 0, 30, and 60 g/kg MOM that corresponded to T1 or control, T2, and T3, separately. Each treatment was divided into 6 replicates of 11 birds apiece, and all birds had access to the outdoors. After 7 weeks of experimental rearing, live weight was 2218.1, 2164.4, and 2176.6 g for birds raised in T1, T2 and T3, respectively, without statistical differences (p ≥ 0.05). Live weight gain and daily live weight gain increased (p ≤ 0.05) in favor of the control group during the first 4 weeks but decreased (p ≤ 0.05) in the last 3 weeks of the trial. Overall feed intake was not influenced by the treatments (p ≥ 0.05). Birds raised with MOM in their diets had higher feed conversion (p ≤ 0.05). T2 diet considerably increased (p ≤ 0.05) birds' breast and gizzard weight and yield while decreasing feet weight and yield, in comparison to T1. Dietary MOM inclusion did not impair or improve (p ≥ 0.05) blood parameters. The results of this study suggest that up to 60 g/kg MOM could be included in the diets of slow-growing Dominant Blue D 107 male chickens grown with access to outdoors without compromising their productive performance (except for feed conversion), slaughter characteristics, and health status.

RESUMO

This study aimed to evaluate the effects of stress in bovines submitted to emergency slaughter caused by lengthy transportation to slaughterhouses in the state of Tocantins, Brazil. Blood collections were performed individually during exsanguinations.Tissue samples were obtained after evisceration on the slaughter line. The animals were divided into the Experimental Group (EG), with 19 bovines destined for immediate emergency slaughter, and the Control Group (CG), with 24 bovines slaughtered normally at the abattoir flow. The EG showed lower values of erythrocytes, hemoglobin, hematocrit, platelets, neutrophilia with band neutrophils, and lymphopenia. Hyperalbuminemia, high levels of total proteins, glucose, and creatinine, and lower urea levels were observed in the EG. The globulins were equal between the groups. Histopathology revealed hepatocytes with vacuolated cytoplasm (87.5% of the cases) and liver congestion (83.33%). Interstitial emphysema (95.65%), alveolar emphysema (65.21%), and congestion (52.17%) were observed in the lungs. The kidneys showed congestion (80.95%) and hyaline casts (100%). The spleens showed rarefaction in the white (70%) and red pulp (65%), in addition to hemosiderosis (10%). This suggests that the animals transported over long distances are submitted to severe stress, with water and food deprivation, resulting in hematological and biochemical changes, with histological lesions and serious bruises affecting the musculoskeletal system. There is loss of homeostasis, severe debilitation, with destination for emergency slaughter with the conditional use of meat for consumption.(AU)

Assuntos

Animais , Estresse Fisiológico , Fenômenos Bioquímicos , Bovinos/lesões , Doenças Hematológicas/diagnóstico , Brasil , Abate de Animais

RESUMO

This study was aimed at determining the effects of dietary zeolite (clinoptilolite) supplementation on fattening performance, slaughter characteristics, and some blood and visceral organ parameters in Japanese quails. For this purpose, 140 (4x35) four-day-old Japanese quail chicks (Coturnix coturnix japonica) were randomly assigned to 4 groups with 4 replicates (9+9+9+8), and the study was continued for a period of 31 days. The groups were fed on a basal diet supplemented with 0% of clinoptilolite (control group), 1.5% of clinoptilolite (Z1.5), 3% of clinoptilolite (Z3) and 6% of clinoptilolite (Z6). For the female quails, the best blood TOS and liver TAS levels were detected in Z3 and Z1.5, respectively. For the male quails, the best blood TAS and liver TOS levels were determined in Z1.5. Histopathological examination demonstrated that dietary supplementation with 1.5% of zeolite had increased the height of the intestinal villi in both male and female quails, and had reduced hepatic lipidosis in female quails. Female quails displayed significantly increased levels of hepatic lipidosis in Z3 and Z6, and liver hepatitis in Z3. In result, it is suggested that zeolite, a hydrated aluminosilicate, in its form containing 90.2% of clinoptilolite, can be incorporated as a feed supplement into quail basal diets at a rate of 3%, owing to its positive effect on fattening performance. Furthermore, the use of zeolite may also contribute to maintaining animal health by reducing humidity in poultry houses and feed.(AU)

Assuntos

Animais , Feminino , Zeolitas/química , Suplementos Nutricionais/efeitos adversos , Coturnix/fisiologia , Fenômenos Fisiológicos Sanguíneos , Vísceras/fisiologia

RESUMO

Calves are susceptible to a wide variety of stressors besides the seasoning low forage availability during their weaning phase. Thus, it is ideal if they receive adequate trace mineral supplementation during this period. This study evaluated the effect of injectable trace minerals (ITM) on performance, concentration of oxidative stress enzymes, and biochemical plasmatic parameters in Nellore calves during the weaning phase and under feed restriction. A total of 30 Nellore calves were used at the age of 7 ± 1 month and 176.23 ± 21.68 kg of initial body weight (BW). These animals were allocated in Urochloa decumbens pasture for 28 days and transferred to Urochloa brizantha cv Marandu pasture for another 28 days. The treatments were: Saline (saline solution; 0.9% NaCl) and ITM (Multimin®, providing: 15 mg Cu/mL, 60 mg Zn/mL, 10 mg Mn/mL, and 5 mg Se/mL). Both treatments were applied subcutaneously in the ratio of 1 mL/45 kg of BW. At the end of the experiment (d 56), animals were lighter than on d 0 in both treatments (P < 0.05). However, calves that received the ITM treatment presented lower body weight (P < 0.05) on d 56, compared to those in the control group. No significant difference (P > 0.05) was observed on the concentrations of oxidative stress enzymes. Moreover, no significant difference (P > 0.05) was observed in the plasmatic parameters; these were at normal range or slightly below the concentrations described for the species. We concluded that, in a feed restriction condition, the use of ITM in weaned calves did not improve performance, and the concentration of oxidative stress enzymes, and biochemistry variables. Therefore, we do not recommend using injectable trace minerals in the concentrations used in this study in animals under such stressful conditions.

Assuntos

Oligoelementos , Ração Animal/análise , Animais , Bovinos , Dieta/veterinária , Suplementos Nutricionais , Desmame

RESUMO

BACKGROUND: A point-of-care device that can provide immediate and reliable hemoglobin (Hb) concentrations and packed cell volumes (PCVs) would be useful in veterinary medicine. OBJECTIVES: We aimed to compare the use of a human device (Mission Plus; MP) with a gold standard (GS) method for measuring Hb concentrations and PCVs in cattle blood. METHODS: Blood samples from clinically healthy cattle (n = 122) were collected with or without an anticoagulant (K2 EDTA). The GS and MP methods were compared with correlation coefficients. Passing-Bablok regression analyses were also performed, and the acceptability judgment was completed using Bland-Altman plots. RESULTS: The CVmax for Hb values obtained using the GS method, the MP device without K2 EDTA, and the MP device with K2 EDTA were approximately 2.70%, 1.70%, and 2.0%, respectively, whereas the CVmax for PCVs was 0.90%, 1.83%, and 2.05%, respectively. A positive correlation (97.5% confidence interval) was observed between the Hb concentrations and PCV values detected using the MP and GS techniques in blood with and without K2 EDTA. Bland-Altman plots showed agreement between the MP and GS methods. For Hb using blood collected with or without the addition of K2 EDTA, the mean differences were -0.87 g/dL (95% CI: 1.35; -3.96) and 0.08 g/dL (95% CI: 2.16, -1.99), respectively. For PCVs using blood collected with or without the addition of K2 EDTA, the mean differences were -3.75% (95% CI: 0.61. -8.12) and -0.88% (95% CI: 2.86, -4.62), respectively. CONCLUSIONS: The MP device can be used to analyze Hb concentrations and PCVs in bovine blood to assist in field diagnoses.

Assuntos

Anticoagulantes , Sistemas Automatizados de Assistência Junto ao Leito , Animais , Bovinos , Tamanho Celular , Hematócrito/veterinária , Hemoglobinas/análise

RESUMO

AIM/BACKGROUND: The most recent pandemic caused by the new coronavirus disease (COVID-19) urged dramatic changes in people's lives. Potentially, the COVID-19 pandemic affects physical and mental health as well as behavioral and social aspects. However, the direct impacts of the COVID-19 pandemic on health-related parameters are not yet known. The present study aimed to evaluate the effect of 16 weeks during the COVID-19 pandemic on health-related parameters of physically inactive women aged 50 to 70 years. METHODS: Thirty-four physically inactive women participated in the study. We performed tests to evaluate aerobic capacity and muscle strength, anthropometric measurements, blood pressure (BP), blood parameters, diet, and physical activity levels. All evaluations were carried out before and 16 weeks after the initial phase of the COVID-19 pandemic in Brazil (i.e., from March to July 2020). RESULTS: Systolic BP (p < .0001; effect size (ES) = 0.62), diastolic BP (p < .0001; ES = 0.71), grip strength of the right (p < .05; ES = 0.43) and left hand (p < .05; ES = 0.49), performance in six-minute walk test (p < .05; ES = 0.46), free time physical activity levels (p < .05; ES = 0.40), domestic physical activity levels (p < .05; ES = 0.39), platelet count (p < .0001; ES = 0.48), and mean corpuscular hemoglobin concentration (p < .0001; ES = 1.14) reduced in comparison to the period before the pandemic. In contrast, glycated hemoglobin levels (p < .0001; ES = 0.77), triglycerides (p < .05; ES = 0.40), and insulin levels (p < .05; ES = 0.60) increased in comparison to the period before the pandemic. CONCLUSION: The COVID-19 pandemic negatively impacted the general health status of physically inactive women aged 50 to 70, potentially increasing their susceptibility to comorbidities, such as type 2 diabetes and hypertriglyceridemia.

Assuntos

COVID-19 , Diabetes Mellitus Tipo 2 , Brasil/epidemiologia , Feminino , Saúde Global , Humanos , Pandemias , SARS-CoV-2

RESUMO

The present study aimed to evaluate the effects of hepatoprotective agents added to diets containing different energy sources on the metabolic status of lactating dairy cows in confinement. Sixteen Holstein × Gir crossbred midlactation cows with a body weight of 553 ± 85 kg were included in this feeding trial. These animals were arranged in a 4 × 4 Latin square experimental design. A 2 × 2 factorial arrangement was employed in this feeding experiment. Experimental treatments consisted of the administration of diets supplemented with a hepatoprotectant and diets without hepatoprotectant. These diets contained varying amounts of ground corn or citrus pulp as energy sources. Serum markers for energy metabolism and proteins were evaluated and liver enzymes were measured. Blood samples were collected in the morning before (0 h) and 4 h after the animals were fed these diets. There was no interaction between the hepatoprotective agent and the dietary energy sources for the metabolic status variables. Ground corn as an energy source yielded higher serum concentrations of glucose, HDL (high-density lipoprotein) cholesterol, and globulins, whereas b-hydroxybutyrate (BHB), non-esterified fatty acids (NEFA), total cholesterol, albumin, and aspartate aminotransferase (AST) yielded the highest concentrations in diets containing citrus pulp. There was interaction between time and hepatoprotective agent in serum LDL (low-density lipoprotein) cholesterol concentration. The present study shows that ground corn diets increased the synthesis of glucogenic nutrients, whereas citrus pulp used as an energy source altered the lipid status of lactating cows. The hepatoprotective agent increased postprandial serum LDL cholesterol levels.

Assuntos

Dieta , Lactação , Ração Animal/análise , Animais , Bovinos , Dieta/veterinária , Suplementos Nutricionais , Metabolismo Energético , Feminino , Leite , Rúmen , Zea mays

RESUMO

Coal extraction and processing generate environmental contamination, which has several negative impacts on human health. Hematological and biochemical parameters are effective biomarkers of the exposure of a population to air pollutants. Thus, this study aimed to evaluate hematological and biochemical parameters in adult residents in a coal mining region in the largest national reserve in Brazil, as well as to investigate their relations with air quality. The study included three cities in the south of Brazil, which are located near an important power plant and coal mine. The air quality was analyzed by PM10, NOx, and SO2 at five air quality monitoring stations, socioeconomic and demographic characteristics were evaluated with a questionnaire, and biochemical parameters were assessed in blood samples. The mean PM10 in the study area was 19.18 µg/m3. The air quality varied among the five monitoring stations, and the Candiota station exceeded the reference limit by 63.3%. In general, participants had lived more than 10 years in the municipality and had hepatic and renal parameters within the reference values. A higher prevalence of alterations was found in hematological parameters (43.1%) and liver function (30%). Among the three cities, the population of Pedras Altas seems to have a greater impairment of the blood parameters evaluated. The only parameter that was correlated with PM10 was the hematocrit level (r = 0.33; P < 0.001).

Assuntos

Poluentes Atmosféricos , Poluição do Ar , Minas de Carvão , Adulto , Poluentes Atmosféricos/análise , Poluição do Ar/análise , Biomarcadores , Brasil , Cidades , Carvão Mineral/análise , Monitoramento Ambiental , Humanos , Material Particulado/análise

RESUMO

This study aimed at evaluating parasitological and blood variables in native breed Crioula Lanada sheep belonging to the same herd, to identify and compare susceptible or resistant individuals to gastrointestinal nematodes during gestation and lactation phases. For this purpose, 18 Crioula sheep were used within 2 years of evaluation, in which blood and feces samples and weight of the animals were taken from their 4th month of gestation until the weaning of their lambs, in the 4th month postpartum. Feces samples were used for counting eggs per gram of feces (EPG) and, thus, to identify 12 resistant ewes (EPG < 1,000) and 6 susceptible (EPG > 1,000) to gastrointestinal nematodes. The identification of infective larvae was also performed. Blood was used for analysis of packed cell volume (PCV), eosinophil count, total plasma protein, and immunoglobulin G level against Haemonchus contortus infective larvae. The Kruskal-Wallis non-parametric comparison test was used to evaluate the differences between days of parturition and linear mixed-effects model using package lme4 in R to evaluate the groups. The main parasite species observed in the feces samples were Haemonchus, Trichostrongylus, Oesophagostomum, Ostertagia, and Cooperia in similar proportions in both groups. Susceptible ewes presented peaks of EPG at the beginning of lactation and lower PCV values throughout the study. No difference between groups was observed concerning other blood variables or body weight, but some changes were observed along with the gestation and lactation phases within each group. The physiological response of sheep to nematode infection is a useful tool to identify the most susceptible individuals within the same breed and herd and to select the most genetically resistant individuals.

RESUMO

The objective of the present study was to determine the associations between the FAMACHA© score, body condition score (BCS) and several haematological parameters of Pelibuey ewes. In total, 52 clinically healthy Pelibuey ewes were evaluated in this study. A blood sample was collected from each animal to determine haematological variables. The BCS was evaluated on a scale of 1 to 5 and subsequently grouped into four categories: (1) < 1.5, (2) 2.0-2.5, (3) 3.0-3.5 and (4) > 4.0. The coloration of conjunctival mucosa was evaluated using the FAMACHA© system. The analysed haematological parameters were red blood cell (RBC) count, haemoglobin (HGB), haematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), red blood cell distribution width standard deviation (RDW-SD) and red blood cell distribution width coefficient of variation (RDW-CV). A descriptive statistical analysis was performed, and the Pearson correlation coefficients between variables were estimated. Haematological parameters were analysed with one-way analyses of variance, and the Tukey test was applied when significant differences were detected. The FAMACHA© score was only associated with MCHC (r = - 0.30, P < 0.05). The haematological parameters of RBC count, HCT, RDW-SD and RDW-CV statistically differed among BCS groups. The BCS was associated with RBC count, HCT, RDW-SD and RDW-CV, with r values ranging from 0.35 to 0.48. The haematological values were similar to those reported in ewes from Iran and Iraq. Hence, the optimization of the BCS calculation of Pelibuey ewes can generate association tables with haematological parameters to aid in the sanitary and feeding management of sheep.

Assuntos

Índices de Eritrócitos , Hemoglobinas , Carneiro Doméstico/fisiologia , Animais , Contagem de Eritrócitos/veterinária , Índices de Eritrócitos/veterinária , Feminino , Hematócrito/veterinária , Hemoglobinas/análise , Irã (Geográfico) , Carneiro Doméstico/sangue

RESUMO

BACKGROUNG: Chronic kidney disease is directly related to cardiovascular disorders. Guided physical exercises significantly improve the adverse effects of dialytic treatmentOBJECTIVE: To analyze changes in biochemical parameters of subjects with chronic kidney disease undergoing moderate exercise during hemodialysisMETHODS: This is an experimental study composed of 54 subjects submitted to hemodialysis, split into a control group and a group with intervention. The experimental group underwent three weekly sessions of aerobic exercise, performed during hemodialysis sessions, with a duration of 30 minutes, for 12 weeks. The blood parameters of both groups were comparedRESULTS: Statistically significant differences were observed between pre (p=0.001) and post-exercise protocol for urea (p=0.006), calcium (p=0.001), alanine aminotransferase (p=0.020) and sodium (p=<0.001). In the control group, we observed significant differences for the calcium variable (p<0.001), alanine aminotransferase (p=0.024), hematocrit (p=0.015), calcium vs phosphorus (p=0.018), and sodium (p=0.023), before and after the periodCONCLUSION: Aerobic training during hemodialysis was able to maintain blood level stability in patients with chronic kidney disease, both during and at the end of the protocol, even considering increased blood flow. This trial is registered in the Brazilian registry of clinical Trials - number RBR-7354r6. : July 5, 2018 at 12:59 PM. : July 24, 2018 at 10:24 AM. Identification of the test - UTN Number: U1111-1216-8272

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INTRODUÇÃO: A doença renal crônica está diretamente relacionada a distúrbios cardiovasculares. Exercícios físicos guiados melhoram significativamente os efeitos adversos do tratamento dialíticoOBJETIVO: Analisar as alterações nos parâmetros bioquímicos de indivíduos com doença renal crônica submetidos a exercícios moderados durante a hemodiáliseMÉTODO: Este é um estudo experimental composto por 54 indivíduos submetidos à hemodiálise, divididos em um grupo controle e um grupo com intervenção. O grupo experimental passou por três sessões semanais de exercício aeróbico, realizadas durante as sessões de hemodiálise, com duração de 30 minutos, por 12 semanas. Os parâmetros sanguíneos de ambos os grupos foram comparadosRESULTADOS: Diferenças estatisticamente significativas foram observadas entre o protocolo pré (p=0,001) e pós-exercício para urea (p=0,006), cálcio (p=0,001), alanina aminotransferase (p=0,020) e sódio (p=&0.001). No grupo controle, observamos diferenças significativas para a variável cálcio (p<0.001), alanina aminotransferase (p=0,024), hematócrito (p=0,015), cálcio vs fósforo (p=0,018) e sódio (p=0,023), antes e depois do períodoCONCLUSÃO: O treinamento aeróbico durante a hemodiálise foi capaz de manter a estabilidade do nível sanguíneo em pacientes com doença renal crônica, tanto durante quanto no final do protocolo, considerando mesmo o aumento do fluxo sanguíneo. Este ensaio está registrado no registro brasileiro de Ensaios Clínicos - número RBR-7354r6. Data de inscrição: 5 de julho de 2018 às 12h59. : 24 de julho de 2018 às 10h24. Identificação do teste - UTN Número: U1111-1216-8272

Assuntos

Humanos , Masculino , Feminino , Circulação Renal , Exercício Físico , Estudos Longitudinais , Diálise Renal , Insuficiência Renal Crônica

RESUMO

Seabirds have been widely used for monitoring the health of the oceans in diverse marine regions. Among low-cost survey strategies, systematic surveys of seabirds beached on coasts have been developed since the 20th century. However, these studies do not always address blood aspects. The assessment of the health status of birds based on the analysis of hematological and plasma chemistry is crucial to evaluate the overall health status profile of live organisms. Here, the authors study the variability of blood parameters by sex, age class, and year of beached Magellanic Penguin during the nonreproductive period in northern Argentina. Of 44 penguins, 77% were categorized as younger juveniles and the rest as older juveniles, and were captured and studied in coastal areas of Buenos Aires Province during the summers of 2017 and 2018. The mean body weight of beached penguins was affected by the age class of the individuals; most of the younger juveniles showed poor condition in terms of body mass (1,761 ± 235 g). No significant differences were observed in body weight between years and sex. Still, there were significant differences between years for alkaline phosphatase (ALP) and creatine phosphokinase (CPK) values. Twelve of the 20 blood parameters analyzed differ significantly with the age class of the beached penguins; younger juveniles were in a state of inanition. Our results may serve as a necessary first step in improving the conservation status of the Magellanic Penguin in nonbreeding grounds of Argentina, and call for a better knowledge of the health status of the species along its annual cycle.

Assuntos

Contagem de Eritrócitos/veterinária , Hematócrito , Contagem de Leucócitos/veterinária , Estações do Ano , Spheniscidae/sangue , Envelhecimento , Fosfatase Alcalina/sangue , Animais , Animais Selvagens , Anticorpos Heterófilos , Argentina , Glicemia , Proteínas Sanguíneas , Colesterol/sangue , Creatina Quinase/sangue , Feminino , Hemoglobinas , Contagem de Linfócitos/veterinária , Masculino , Fósforo/sangue , Transaminases/sangue , Ureia/sangue , Ácido Úrico/sangue

RESUMO

The use of additives such as ractopamine (Rac) in pregnant sows during early-mid pregnancy is an alternative to increase foetal and progeny growth and development. However, Rac supplementation in finishing pigs can lead to behavioural and physiological changes similar to the typical stress responses. The objective of this study was to evaluate the effects of dietary supplementation with Rac in pregnant sows from day 25 to 50 of gestation (pre-hyperplastic stage) on piglet's vitality, blood parameters, number, diameter and perimeter of muscle fibres in semitendinosus muscle and developmental characteristics of piglets at birth to weaning. Forty-one hybrid sows were divided into three dietary treatments: (1) control diet without Rac (control), (2) addition of 10 mg/kg of Rac (Rac10) and (3) addition of 20 mg/kg of Rac (Rac20). Higher numbers of low-vitality piglets (P<0.05) were observed in Rac-fed sows, regardless of dose, compared with the control group. Very low-density lipoprotein levels were lower in the Rac10 group when compared with the Rac20 group at day 21. Haematocrit was greater, and the mean corpuscular haemoglobin concentration was lower in piglets from Rac-fed sows. No significant statistical differences were detected regarding piglets body weight, average daily gain, blood gasometry, complete blood count and muscle fibre measurements in semitendinosus muscle. The use of Rac in pregnant sows reduced the vitality parameters of piglets but did not improve the performance from birth until weaning and did not negatively influence the haematological parameter and lipid metabolism.

Assuntos

Fenetilaminas/metabolismo , Sus scrofa/fisiologia , Ração Animal/análise , Animais , Animais Recém-Nascidos/sangue , Animais Recém-Nascidos/fisiologia , Dieta/veterinária , Suplementos Nutricionais/análise , Feminino , Fenetilaminas/administração & dosagem , Gravidez , Sus scrofa/sangue

RESUMO

El EDTA es el anticoagulante de elección en los laboratorios de hematología para la conservación de la muestra de sangre total. Existen dos tipos, EDTA K2 y EDTA K3, y su diferencia radica en la cantidad de moléculas de potasio. Algunas guías sugieren que hay diferencias entre el anticoagulante EDTA K2 y el K3 para el proceso del hemograma; sin embargo, con las nuevas presentaciones de los tubos que traen las casas comerciales, no se tiene claro si en realidad aún hay diferencia entre los dos anticoagulantes, y si esto puede alterar el resultado del hemograma, tanto en el resultado cuantitativo, como en el cualitativo. Objetivo. Comparar los recuentos leucocitarios, la hemoglobina, el hematocrito, el volumen corpuscular medio, las plaquetas y la morfología celular en muestras de sangre periférica con EDTA K2 y EDTA K3, en diferentes tiempos (0, 1 y 2 horas). Materiales y métodos. Se realizó un estudio cuasi-experimental, multivariado, multifactorial, que tiene como unidad de análisis la sangre anticoagulada con EDTA K2 y EDTA K3, extraída de 53 individuos a través de un muestreo no probabilístico por conveniencia. Resultados. Al comparar los resultados del estudio morfológico por medio del extendido de sangre periférica y los datos cuantitativos del hemograma, se encontró que no hay diferencias estadísticamente significativas usando EDTA K2 o K3. Conclusión. Se evidenció que el uso del EDTA K2 o EDTA K3 como anticoagulante de elección, procesando las muestras en un tiempo adecuado después de su recolección, no afecta los parámetros cuantitativos del hemograma automatizado ni los morfológicos.

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EDTA is the anticoagulant of choice in hematology laboratories for the conservation of whole blood samples. There are two types, K2 EDTA and K3 EDTA, and their difference lies in the amount of potassium molecules. Some guidelines suggest that there are differences between K2 and K3 EDTA for the blood analysis process. However, with the new collection tubes offered by the commercial suppliers, it is not clear if in fact there is a difference between the two anticoagulants that would result in changes in blood parameters and cell morphology. Objective. To compare leukocyte counts, hemoglobin, hematocrit, mean corpuscular volume, platelets and cell morphology in peripheral blood samples collected with K2 EDTA and K3 EDTA, at different times (0, 1 and 2 hours). Materials and methods. A quasi-experimental, multivariate, multifactorial study was carried out, with anticoagulated blood as the unit of analysis, either with K2 EDTA or K3 EDTA, extracted from 53 subjects through a non-probabilistic sampling for convenience. Results. There was no statistically significant difference when comparing results of the peripheral blood smear and the quantitative hematological parameters using K2 or K3 EDTA. Conclusion. The use of either K2 EDTA or K3 EDTA as the anticoagulant of choice, when processing samples within a suitable time after their collection, proved equally satisfactory for both quantitative and morphological parameters

Assuntos

Humanos , Células Sanguíneas , Contagem de Células Sanguíneas , Ácido Edético