RESUMO
For comprehensive gene expression analyses of the phytopathogenic fungus Botrytis cinerea, which infects a number of plant taxa and is a cause of substantial agricultural losses worldwide, we developed BEB, a web-based B. cinerea gene Expression Browser. This computationally inexpensive web-based application and its associated database contain manually curated RNA-Seq data for B. cinerea. BEB enables expression analyses of genes of interest under different culture conditions by providing publication-ready heatmaps depicting transcript levels, without requiring advanced computational skills. BEB also provides details of each experiment and user-defined gene expression clustering and visualization options. If needed, tables of gene expression values can be downloaded for further exploration, including, for instance, the determination of differentially expressed genes. The BEB implementation is based on open-source computational technologies that can be deployed for other organisms. In this case, the new implementation will be limited only by the number of transcriptomic experiments that are incorporated into the platform. To demonstrate the usability and value of BEB, we analyzed gene expression patterns across different conditions, with a focus on secondary metabolite gene clusters, chromosome-wide gene expression, previously described virulence factors, and reference genes, providing the first comprehensive expression overview of these groups of genes in this relevant fungal phytopathogen. We expect this tool to be broadly useful in B. cinerea research, providing a basis for comparative transcriptomics and candidate gene identification for functional assays.
RESUMO
Abstract There is no document that analyzes the state of the art of scientific research published between 2013 and 2018 in relation to possible interdependence links between emotional regulation [ER] and healthy behaviors associated with Body Energy Balance [BEB] (physical activity, balanced diet and sleep hygiene). To achieve this purpose, an exploratory systematic review was conducted, whose search criteria were "emotion regulation", "emotion dysregulation" connected with the Boolean operator "AND" to the keywords "sleep hygiene", "eating behavior" and "physical activity". Terms like "alexithymia", "depression", "stress", "negative emotions", and "rumination" were omitted. The methodological quality of the evidence was assessed with a patented rubric. After applying the analysis criteria, 35 articles were obtained, reporting the existence of reciprocal associations and interactions between ER and at least one of the three behaviors associated with BEB were analyzed. It is concluded that, despite the importance of these behaviors in the vital maintenance of people and the clear impact that emotional regulation has on them, their research has not been sufficient, and more empirical studies in this regard in Latin America are needed.
Resumen No existe en la actualidad un documento que analice el estado del conocimiento de las investigaciones científicas publicadas en los últimos años con respecto a los posibles vínculos de interdependencia entre la regulación emocional (RE) y las conductas asociadas al balance energético corporal (BEC) -actividad física, alimentación balanceada e higiene del sueño-. Por tanto, para lograr dicho propósito, en el presente estudio se realizó una revisión sistemática exploratoria, cuyos criterios de búsqueda fueron los términos "emotion regulation" y "emotion dysregulation", conectados con el operador booleano "AND" a las palabras clave "sleep hygiene", "eating behavior" y "physical activity" -términos como "alexithymia", "depression", "stress", "negative emotions", y "rumination" fueron omitidos-; donde se valoró la calidad metodológica de la evidencia de los artículos a partir una rúbrica patentada. Tras aplicar los criterios de inclusión establecidos, se obtuvo un total de 35 artículos que reportaban la existencia de asociaciones e interacciones recíprocas entre la RE y al menos una de las tres conductas asociadas al BEC. Como resultado, se puede afirmar que, a pesar de la importancia de dichas conductas en el mantenimiento vital de las personas y el claro impacto que tiene la regulación emocional sobre ellas, su estudio no ha sido suficiente, y por tanto se necesitan más investigaciones empíricas en el contexto latinoamericano.
RESUMO
Este trabalho parte de dois postulados para pensar a intervenção sobre a posição subjetiva materna na clínica psicanalítica com bebês. O primeiro postulado é que o campo de desenvolvimento da criança interessa à psicanílise por ter como base uma teorização e uma forma de compreensão da construção subjetiva; e o segundo postulado é que a psicanálise remete a uma clínica do sujeito. O artigo mostra como o discurso psicanalítico ordena o lugar do sujeito em sua interlocução linguageira, ou seja, a sua submissão aos significantes que o determinam e a marca da completude impossível, ou ainda, que o bebê, antes de nascer, é um significante.
This paper presents two postulates to think the intervention on the subjective position maternal about the psychoanalytical clinic with babies. The first postulate is that the of development of the child interests the psychoanalysis for having as base a theorization and a form of understanding of the subjective construction; and the other postulate are that the psychoanalysis sends to a clinic of the subject. The article shows as the psychoanalytical speech commands the place of the subject in its language interlocution, that is, its submission to the significant that they determine it and the mark of the impossible completion, or still, that the baby, before being born, is a significant.
Este artículo presenta dos postulados para pensar la intervención en la posición subjetiva materna en la clínica psicoanalítica con los bebés. El primer postulado es que el campo del desarrollo del ni?o interesa a psicoanalisis por tener como base una teorización y una forma de comprensión de la construcción subjetiva; y lo segundo postulado es que el psicoanalisis envía a una clínica del sujeto. El artículo demuestra como el discurso psicoanalítico ordena el lugar del sujeto en su interlocution de la lengua, es decir, su sumisión al significante que lo determinan y la marca de la completude imposible, o aún, que el bebé?, antes de nacer, es un significante.