RESUMO

OBJECTIVE: To assess the reproducibility of using AutoCAD software to measure the area of venous leg ulcers (VLUs). METHOD: Data from patients with VLUs were collected between March and July 2015, using data collection forms and photographing the different ulcers. A researcher and five nurses collected the data. The wounds were measured using AutoCAD software. Data were analysed using intraclass correlation coefficient (ICC), concordance correlation coefficient (CCC) and Bland-Altman analysis. RESULTS: A total of 21 patients with 36 VLUs were included in the study. A statistically significant difference (p<0.05) was observed between the areas of VLUs measured by the researcher and the evaluators. There was an excellent agreement when analysing the ICC [p=0.98; 95% CI (0.97-0.99); p <0.05] and the CCC [CCC=CI 0.97; 95% (0.95-0.98)]. There was no difference between the measurements of VLUs ≤10 cm2 (p=0.49) and those with an area >10cm2 (p=0.22). CONCLUSION: The use of AutoCAD software is appropriate for measuring VLUs and appears to be more accurate when used to measure VLUs with an area >10cm2.

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RESUMO

The determination of loin eye area (LEA) is one of the most common methods to assess carcass quality and it is possible to adjust calculations to predict meat amount in the carcass which is one of the important features in the classification process. The aim of this study was to compare different methodologies used to determine LEA in swine. Fifteen crossbred pigs, Landrace x Large White, males and females, were slaughtered at 140 days of age. After 24 hours of cooling at 4°C, the Longissimus dorsi muscle was removed from the left half part of carcasses for evaluation of LEA between the 12th and 13th rib. The methods were: point counting on plastic grid of 1 cm2 and 0.25 cm2 (PCGP 1 cm2 and PCGC 0.25 cm2 ), circumference method by Echo Image Viewer (ECHO), circumference method using AutoCad ® program (CAD) and method of weighing paper (WP). The design was completely randomized with 15 replications per treatment and the data were subjected to Pearson correlation and variance analysis. Averages were compared by Tukey test at a significance level of 5%. LEA values were: 35.84, 35.69, 33.86, 34.22 and 36.71 cm2 , for the PCGP 1 cm2 , PCGP 0.25 cm2 , ECHO, CAD and WP methods, respectively. The LEA values determined by methods PCGP 1 cm2 , PCGP 0.25 cm2 and WP were similar; however they were higher than those obtained by the ECHO and CAD methodologies (P<0.0001). PCGP 1 cm2 , PCGP 0.25 cm2 and WP methods may overestimate LEA values. The choice of method to be used should be based on its practicality and availability of resources, since the difference obtained between them is low.

A determinação da área de olho de lombo (LEA) é o método mais comum para análises de qualidade de carcaça, sendo possível ajustar cálculos para predizer o grau de musculosidade na mesma, sendo um recurso importante nos processos de classificação. Objetivou-se com este trabalho comparar diferentes metodologias utilizadas na determinação da LEA em suínos. Quinze suínos mestiços Landrace x Large White, machos e fêmeas, foram abatidos aos 140 dias de idade. Após 24 horas de refrigeração a 4°C o músculo Longissimus dorsi foi retirado da meia carcaça esquerda para avaliação da LEA entre a 12ª e 13ª costela. Os métodos utilizados para avaliação foram: contagem de pontos em gabarito plástico de 1 cm² e de 0,25 cm2 (CPGP 1 cm2 e CPGC 0,25 cm2 ), método de circunferência por meio do programa Echo Image Viewer (ECHO), método de circunferência por meio do programa AutoCad® (CAD) e método de pesagem do papel (WP). O delineamento foi inteiramente casualizado, com 15 repetições por tratamento e os dados foram submetidos a teste de correlação e análise de variância. As médias foram comparadas pelo teste de Tukey, ao nível de 5% de significância. Os valores médios de LEA observados foram de 35,84, 35,69, 33,86, 34,22 e 36,71 cm2, para os métodos CPGP 1 cm2, CPGP 0,25 cm2 , ECHO, CAD e WP, respectivamente. Os valores de LEA determinados pelos métodos CPGP 1 cm2, CPGP 0,25 cm2 e WP foram semelhantes entre si, entretanto superiores aos obtidos pelas metodologias ECHO e CAD (P<0,0001). Os métodos CPGP 1 cm2 , CPGP 0,25 cm2 e WP podem superestimar os valores de LEA. A escolha do método a ser utilizado deve ser feita com base em sua praticidade e disponibilidade de recursos, uma vez que a diferença entre eles é de pequena ordem.

Assuntos

RESUMO

The determination of loin eye area (LEA) is one of the most common methods to assess carcass quality and it is possible to adjust calculations to predict meat amount in the carcass which is one of the important features in the classification process. The aim of this study was to compare different methodologies used to determine LEA in swine. Fifteen crossbred pigs, Landrace x Large White, males and females, were slaughtered at 140 days of age. After 24 hours of cooling at 4°C, the Longissimus dorsi muscle was removed from the left half part of carcasses for evaluation of LEA between the 12th and 13th rib. The methods were: point counting on plastic grid of 1 cm2 and 0.25 cm2 (PCGP 1 cm2 and PCGC 0.25 cm2 ), circumference method by Echo Image Viewer (ECHO), circumference method using AutoCad ® program (CAD) and method of weighing paper (WP). The design was completely randomized with 15 replications per treatment and the data were subjected to Pearson correlation and variance analysis. Averages were compared by Tukey test at a significance level of 5%. LEA values were: 35.84, 35.69, 33.86, 34.22 and 36.71 cm2 , for the PCGP 1 cm2 , PCGP 0.25 cm2 , ECHO, CAD and WP methods, respectively. The LEA values determined by methods PCGP 1 cm2 , PCGP 0.25 cm2 and WP were similar; however they were higher than those obtained by the ECHO and CAD methodologies (P<0.0001). PCGP 1 cm2 , PCGP 0.25 cm2 and WP methods may overestimate LEA values. The choice of method to be used should be based on its practicality and availability of resources, since the difference obtained between them is low.

A determinação da área de olho de lombo (LEA) é o método mais comum para análises de qualidade de carcaça, sendo possível ajustar cálculos para predizer o grau de musculosidade na mesma, sendo um recurso importante nos processos de classificação. Objetivou-se com este trabalho comparar diferentes metodologias utilizadas na determinação da LEA em suínos. Quinze suínos mestiços Landrace x Large White, machos e fêmeas, foram abatidos aos 140 dias de idade. Após 24 horas de refrigeração a 4°C o músculo Longissimus dorsi foi retirado da meia carcaça esquerda para avaliação da LEA entre a 12ª e 13ª costela. Os métodos utilizados para avaliação foram: contagem de pontos em gabarito plástico de 1 cm² e de 0,25 cm2 (CPGP 1 cm2 e CPGC 0,25 cm2 ), método de circunferência por meio do programa Echo Image Viewer (ECHO), método de circunferência por meio do programa AutoCad® (CAD) e método de pesagem do papel (WP). O delineamento foi inteiramente casualizado, com 15 repetições por tratamento e os dados foram submetidos a teste de correlação e análise de variância. As médias foram comparadas pelo teste de Tukey, ao nível de 5% de significância. Os valores médios de LEA observados foram de 35,84, 35,69, 33,86, 34,22 e 36,71 cm2 , para os métodos CPGP 1 cm2 , CPGP 0,25 cm2 , ECHO, CAD e WP, respectivamente. Os valores de LEA determinados pelos métodos CPGP 1 cm2 , CPGP 0,25 cm2 e WP foram semelhantes entre si, entretanto superiores aos obtidos pelas metodologias ECHO e CAD (P<0,0001). Os métodos CPGP 1 cm2 , CPGP 0,25 cm2 e WP podem superestimar os valores de LEA. A escolha do método a ser utilizado deve ser feita com base em sua praticidade e disponibilidade de recursos, uma vez que a diferença entre eles é de pequena ordem.

Assuntos

RESUMO

The determination of loin eye area (LEA) is one of the most common methods to assess carcass quality and it is possible to adjust calculations to predict meat amount in the carcass which is one of the important features in the classification process. The aim of this study was to compare different methodologies used to determine LEA in swine. Fifteen crossbred pigs, Landrace x Large White, males and females, were slaughtered at 140 days of age. After 24 hours of cooling at 4°C, the Longissimus dorsi muscle was removed from the left half part of carcasses for evaluation of LEA between the 12th and 13th rib. The methods were: point counting on plastic grid of 1 cm2 and 0.25 cm2 (PCGP 1 cm2 and PCGC 0.25 cm2 ), circumference method by Echo Image Viewer (ECHO), circumference method using AutoCad ® program (CAD) and method of weighing paper (WP). The design was completely randomized with 15 replications per treatment and the data were subjected to Pearson correlation and variance analysis. Averages were compared by Tukey test at a significance level of 5%. LEA values were: 35.84, 35.69, 33.86, 34.22 and 36.71 cm2 , for the PCGP 1 cm2 , PCGP 0.25 cm2 , ECHO, CAD and WP methods, respectively. The LEA values determined by methods PCGP 1 cm2 , PCGP 0.25 cm2 and WP were similar; however they were higher than those obtained by the ECHO and CAD methodologies (P<0.0001). PCGP 1 cm2 , PCGP 0.25 cm2 and WP methods may overestimate LEA values. The choice of method to be used should be based on its practicality and availability of resources, since the difference obtained between them is low.(AU)

A determinação da área de olho de lombo (LEA) é o método mais comum para análises de qualidade de carcaça, sendo possível ajustar cálculos para predizer o grau de musculosidade na mesma, sendo um recurso importante nos processos de classificação. Objetivou-se com este trabalho comparar diferentes metodologias utilizadas na determinação da LEA em suínos. Quinze suínos mestiços Landrace x Large White, machos e fêmeas, foram abatidos aos 140 dias de idade. Após 24 horas de refrigeração a 4°C o músculo Longissimus dorsi foi retirado da meia carcaça esquerda para avaliação da LEA entre a 12ª e 13ª costela. Os métodos utilizados para avaliação foram: contagem de pontos em gabarito plástico de 1 cm² e de 0,25 cm2 (CPGP 1 cm2 e CPGC 0,25 cm2 ), método de circunferência por meio do programa Echo Image Viewer (ECHO), método de circunferência por meio do programa AutoCad® (CAD) e método de pesagem do papel (WP). O delineamento foi inteiramente casualizado, com 15 repetições por tratamento e os dados foram submetidos a teste de correlação e análise de variância. As médias foram comparadas pelo teste de Tukey, ao nível de 5% de significância. Os valores médios de LEA observados foram de 35,84, 35,69, 33,86, 34,22 e 36,71 cm2 , para os métodos CPGP 1 cm2 , CPGP 0,25 cm2 , ECHO, CAD e WP, respectivamente. Os valores de LEA determinados pelos métodos CPGP 1 cm2 , CPGP 0,25 cm2 e WP foram semelhantes entre si, entretanto superiores aos obtidos pelas metodologias ECHO e CAD (P<0,0001). Os métodos CPGP 1 cm2 , CPGP 0,25 cm2 e WP podem superestimar os valores de LEA. A escolha do método a ser utilizado deve ser feita com base em sua praticidade e disponibilidade de recursos, uma vez que a diferença entre eles é de pequena ordem.(AU)

Assuntos

RESUMO

The determination of loin eye area (LEA) is one of the most common methods to assess carcass quality and it is possible to adjust calculations to predict meat amount in the carcass which is one of the important features in the classification process. The aim of this study was to compare different methodologies used to determine LEA in swine. Fifteen crossbred pigs, Landrace x Large White, males and females, were slaughtered at 140 days of age. After 24 hours of cooling at 4°C, the Longissimus dorsi muscle was removed from the left half part of carcasses for evaluation of LEA between the 12th and 13th rib. The methods were: point counting on plastic grid of 1 cm2 and 0.25 cm2 (PCGP 1 cm2 and PCGC 0.25 cm2 ), circumference method by Echo Image Viewer (ECHO), circumference method using AutoCad ® program (CAD) and method of weighing paper (WP). The design was completely randomized with 15 replications per treatment and the data were subjected to Pearson correlation and variance analysis. Averages were compared by Tukey test at a significance level of 5%. LEA values were: 35.84, 35.69, 33.86, 34.22 and 36.71 cm2 , for the PCGP 1 cm2 , PCGP 0.25 cm2 , ECHO, CAD and WP methods, respectively. The LEA values determined by methods PCGP 1 cm2 , PCGP 0.25 cm2 and WP were similar; however they were higher than those obtained by the ECHO and CAD methodologies (P<0.0001). PCGP 1 cm2 , PCGP 0.25 cm2 and WP methods may overestimate LEA values. The choice of method to be used should be based on its practicality and availability of resources, since the difference obtained between them is low.(AU)

A determinação da área de olho de lombo (LEA) é o método mais comum para análises de qualidade de carcaça, sendo possível ajustar cálculos para predizer o grau de musculosidade na mesma, sendo um recurso importante nos processos de classificação. Objetivou-se com este trabalho comparar diferentes metodologias utilizadas na determinação da LEA em suínos. Quinze suínos mestiços Landrace x Large White, machos e fêmeas, foram abatidos aos 140 dias de idade. Após 24 horas de refrigeração a 4°C o músculo Longissimus dorsi foi retirado da meia carcaça esquerda para avaliação da LEA entre a 12ª e 13ª costela. Os métodos utilizados para avaliação foram: contagem de pontos em gabarito plástico de 1 cm² e de 0,25 cm2 (CPGP 1 cm2 e CPGC 0,25 cm2 ), método de circunferência por meio do programa Echo Image Viewer (ECHO), método de circunferência por meio do programa AutoCad® (CAD) e método de pesagem do papel (WP). O delineamento foi inteiramente casualizado, com 15 repetições por tratamento e os dados foram submetidos a teste de correlação e análise de variância. As médias foram comparadas pelo teste de Tukey, ao nível de 5% de significância. Os valores médios de LEA observados foram de 35,84, 35,69, 33,86, 34,22 e 36,71 cm2, para os métodos CPGP 1 cm2, CPGP 0,25 cm2 , ECHO, CAD e WP, respectivamente. Os valores de LEA determinados pelos métodos CPGP 1 cm2, CPGP 0,25 cm2 e WP foram semelhantes entre si, entretanto superiores aos obtidos pelas metodologias ECHO e CAD (P<0,0001). Os métodos CPGP 1 cm2 , CPGP 0,25 cm2 e WP podem superestimar os valores de LEA. A escolha do método a ser utilizado deve ser feita com base em sua praticidade e disponibilidade de recursos, uma vez que a diferença entre eles é de pequena ordem.(AU)

Assuntos

RESUMO

Aims: The quantitative measurement and characterization of microscopical images using a computer is histomorphometry. The purpose of this experimental research is to evaluate the validity of two methods of histomorphometry of root structure loss measuring antiresorptive efficacy of topical application of alendronate in delayed tooth replantation on a canine model. Methods: We used fortyeight premolar mature roots from five dogs in the study. Roots were soaked in: 1 mMol of ALN, 3 mMol of ALN and physiological saline. After 4 months, animals were sacrificed and a bone block was prepared for analysis. Histological slides were photographed with digital camera (Olympus C 5060 5.1 megapixel) which is directly attached to microscope (Carl Zeiss, Axiolab, Jena, Germany) connected to a PC. The images obtained were stored as figures (TIFF) for further interpretation. Root structure loss due to resorption was measured in two ways. Results: The t-test for two independent samples for evaluating root mass loss between different methods of measurement was used. (t=4.497; df=17; p<0.05). Conclusion: The obtained results showed additional accuracy and precision by computer method that enables future researchers in the area of traumatic injuries of dento-alveolar system, for applying in pathohistology scientific approach, and for measuring the resorption changes on the roots of replanted teeth.

Assuntos