RESUMO
Arctium lappa L., also known as burdock, is an edible wild plant which has the ability to grow in distinct environments and is considered a weed in several parts of the world. This species has great value in the biological and medical fields with its major secondary components being phenolic compounds and terpenes, substances rich in desired biological activities as antioxidant, antimicrobial, antitumor and anti-inflammatory. In addition, burdock leaves extracts have shown a modulatory effect on the complement system, which plays an important role in the development of inflammatory diseases, with an inhibitory effect on all complement pathways. Thus, natural products with those relevant activities are promising agents for healthcare applications. Therefore, the species A.â lappa may represent an interesting asset for researching and developing new therapies for inflammatory afflictions.
Assuntos
Arctium , Arctium/química , Extratos Vegetais/química , Antioxidantes/química , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/metabolismo , Compostos Fitoquímicos/farmacologia , Compostos Fitoquímicos/metabolismoRESUMO
In this present study, we investigated the influence of various extraction methods including maceration, sonication, infusion, decoction, and microwave extraction, on the chemical and biological potential of phytochemicals extracted from three medicinal plants (Ageratum conyzoides, Plantago majorand Arctium lappa L). The results were subsequently analyzed by variance analysis. Our results suggested that sonication is the most effective extraction method among the five methods tested herein, for the extraction of phytochemicals that have a high antioxidant potential and high phenolic content. The three plants employed for this study had a high concentration of flavonoids and phenolics which was compatible with the chemosystematics of the species. All the samples possessed a Sun Protection Factor (SPF) of less than 6. Interestingly, a maximum reaction time of approximately 20 min was noted for the complexation of AlCl3 with the flavonoids present in the phytochemical extract during analyses of the kinetic parameters. We finally identified that the Ageratum conyzoides extract, prepared by sonication, possessed a significant pharmacological potential against hepatocarcinoma tumour cells, whose result can guide further studies for its therapeutic efficacy.
En el presente estudio, investigamos la influencia de varios métodos de extracción, incluyendo maceración, sonicación, infusión, decocción y extracción por microondas, sobre el potencial químico y biológico de los fitoquímicos extraídos de tres plantas medicinales (Ageratum conyzoides, Plantago majory Arctium lappa L). Los resultados se analizaron posteriormente mediante análisis de varianza. Nuestros resultados sugieren que la sonicación es el método de extracción más eficaz entre los cinco métodos aquí probados, para la extracción de fitoquímicos que tienen un alto potencial antioxidante y un alto contenido fenólico. Las tres plantas empleadas para este estudio tenían una alta concentración de flavonoides y fenólicos que era compatible con la quimiosistemática de las especies. Todas las muestras poseían un factor de protección solar (SPF) menor a 6. Curiosamente, se observó un tiempo máximo de reacción de aproximadamente 20 min para la complejación de AlCl3con los flavonoides presentes en el extracto fitoquímico durante los análisis de los parámetros cinéticos. Finalmente, identificamos que el extracto de Ageratum conyzoides, elaborado por sonicación, posee un importante potencial farmacológico frente a las células tumorales del hepatocarcinoma, cuyo resultado puede orientar nuevos estudios sobre su eficacia terapéutica.
Assuntos
Plantas Medicinais/química , Compostos Fitoquímicos/isolamento & purificação , Fenóis/isolamento & purificação , Plantago/química , Flavonoides/isolamento & purificação , Sobrevivência Celular , Análise de Variância , Ageratum/química , Arctium/químicaRESUMO
Nonalcoholic steatohepatitis (NASH) is considered growing risk factor for hepatocellular carcinoma development in high-income countries. Diet- and chemically induced rodent models have been applied for the translational study of NASH-associated hepatocarcinogenesis due to their morphological and molecular similarities to the corresponding human disease. Arctium lappa L. (burdock) root tea has been extensively consumed in Traditional Chinese Medicine due to its potential therapeutic properties. Indeed, the bioactive compounds of A. lappa root, as the polyphenols, have already showed antioxidant and anti-inflammatory properties in different in vivo and in vitro bioassays. In this study, we investigated whether burdock root ethanolic extract (BRE) administration attenuates NASH-associated hepatocarcinogenesis. Eight-week-old male Wistar rats received choline-deficient high-fat diet for 8 weeks and multiple thioacetamide doses for 4 weeks in order to induce NASH and preneoplastic glutathione-S-transferase pi (GST-P)+ preneoplastic foci. Subsequently, rats were treated with BRE (100 or 200 mg/kg body weight) or vehicle by oral gavage for 2 weeks. BRE displayed high levels of chlorogenic and caffeic acids and BRE administration reduced total fatty acid and lipid hydroperoxide levels, while increasing the activities of antioxidant superoxide dismutase and catalase enzymes in the liver. Furthermore, burdock intervention diminished the size of GST-P+ remodeling preneoplastic lesions (PNLs) and displayed a trend on reducing hepatocyte proliferation (Ki-67) inside them. These findings suggest that short-term exposure to BRE alleviated remodeling PNL development in NASH-associated hepatocarcinogenesis.
Assuntos
Anti-Inflamatórios/uso terapêutico , Antioxidantes/uso terapêutico , Arctium/química , Neoplasias Hepáticas/prevenção & controle , Hepatopatia Gordurosa não Alcoólica/tratamento farmacológico , Extratos Vegetais/uso terapêutico , Lesões Pré-Cancerosas/prevenção & controle , Animais , Anti-Inflamatórios/isolamento & purificação , Antioxidantes/isolamento & purificação , Antioxidantes/metabolismo , Ácidos Cafeicos , Dieta Hiperlipídica/efeitos adversos , Humanos , Neoplasias Hepáticas/patologia , Masculino , Medicina Tradicional Chinesa , Hepatopatia Gordurosa não Alcoólica/patologia , Extratos Vegetais/isolamento & purificação , Raízes de Plantas/química , Lesões Pré-Cancerosas/patologia , Ratos , Ratos Wistar , Tioacetamida/toxicidadeRESUMO
The complement system participates in host defense by eliminating microorganisms and triggering inflammation. However, insufficient control or exacerbated complement activation contributes to inflammatory diseases. Since promising antioxidant and anti-inflammatory activities have been identified in Arctium lappa L. extracts, this study aims to explore the effect of A. lappa extracts on the lectin pathway (LP) of complement activation. Four extracts were obtained by supercritical extraction using scCO2 with or without ethanol as co-solvent, at different temperatures and pressures (E1: 2.2â mg/mL, E2: 2.6â mg/mL and E3: 2.0â mg/mL, E4: 1.5â mg/mL). To evaluate the effect of A. lappa extracts on the LP activation, an ELISA assay using mannose binding lectin pathway of complement was carried out with C4 detection. All extracts showed a concentration-dependent inhibitory effect on the activation of complement by the LP. The following IC50 were observed for E1, E2, E3 and E4: 179.4â µg/mL, 74.69â µg/mL, 119.1â µg/mL and 72.19â µg/mL, respectively. Our results suggest that A. lappa extracts are potential candidates for the treatment of inflammatory disorders that are complement-related.
Assuntos
Arctium/química , Cromatografia com Fluido Supercrítico/métodos , Proteínas do Sistema Complemento/metabolismo , Lectinas/metabolismo , Extratos Vegetais/química , Arctium/metabolismo , Dióxido de Carbono/química , Proteínas do Sistema Complemento/agonistas , Lectinas/antagonistas & inibidores , Folhas de Planta/química , Folhas de Planta/metabolismo , TemperaturaRESUMO
Background: Arctium lappa has been used as popular medicinal herb and health supplement in Chinese societies. Bioactive components from A. lappa have attracted the attention of researchers due to their promising therapeutic effects. In this study, we investigated the effects of A. lappa hydroalcoholic extract (Alhe) during different models of inflammation, in vivo. Methods: The anti-inflammatory activity was evaluated through the air pouch model. For this, mice received an inflammatory stimulus with lipopolysaccharide (LPS) and were later injected with Alhe. To assess anti-tumoral activity, the animals were inoculated with B16F10 cells and injected with Alhe every 5 days, along the course of 30 days. Controls were submitted to the same conditions and injected with the vehicle. Peritoneal or air pouch fluids were collected to evaluate leukocyte counting or cellular activation via quantification of cytokines and nitric oxide. Results: Alhe injection reduced the neutrophil influx and production of inflammatory mediators in inflammatory foci after LPS or tumor challenges. Furthermore, Alhe injection reduced tumor growth and enhanced mice survival. Conclusions: Collectively, these data suggest that Alhe regulates immune cell migration and activation, which correlates with favorable outcome in mouse models of acute inflammation and melanoma progression.
RESUMO
The plant Arctium lappa L. is popularly used to relieve symptoms of inflammatory disorders. A crude polysaccharide fraction (SAA) resulting of aqueous extraction of A. lappa leaves showed a dose dependent anti-edematogenic activity on carrageenan-induced paw edema, which persisted for up to 48h. Sequential fractionation by ultrafiltration at 50kDa and 30kDa cut-off membranes yielded three fractions, namely RF50, RF30, and EF30. All these maintained the anti-edematogenic effect, but RF30 showed a more potent action, inhibiting 57% of the paw edema at a dose of 4.9mg/kg. The polysaccharide RF30 contained galacturonic acid, galactose, arabinose, rhamnose, glucose, and mannose in a 7:4:2:1:2:1 ratio and had a Mw of 91,000g/mol. Methylation analysis and NMR spectroscopy indicated that RF30 is mainly constituted by a type I rhamnogalacturonan branched by side chains of types I and II arabinogalactans, and arabinan.
Assuntos
Arctium/química , Polissacarídeos/química , Polissacarídeos/farmacologia , Animais , Espectroscopia de Ressonância Magnética Nuclear de Carbono-13 , Fracionamento Químico , Cromatografia em Gel , Edema/tratamento farmacológico , Edema/patologia , Feminino , Camundongos , Monossacarídeos/análise , Polissacarídeos/uso terapêutico , Espectroscopia de Prótons por Ressonância MagnéticaRESUMO
The protective role of Arctium lappa (AL) on the testes of rats acutely exposed to cadmium (Cd) was tested. The rats were randomly divided into a control group (C-group) and three major experimental groups, which were further subdivided into minor groups (n = 6) according to the experimental period (7 or 56 days). The C-group was subdivided into C-7 and C-56 [receiving a single saline solution, intraperitoneal (i.p.), on the first day]; the AL-group, AL-7, and AL-56, received AL extract (300 mg/kg/daily); the Cd group, Cd-7 and Cd-56, received a single i.p. dose of CdCl2 (1.2 mg/kg body weight (BW)) on the first day; the CdAL group, CdAL-7 and CdAL-56, received the same Cd dose, followed by AL extract. Water or AL extract was administered daily by gavage. After either 7 or 56 days, the testis and accessory glands were removed after whole-body perfusion. Exposure to Cd and CdAL decreased the weight of the testis and epididymis, the gonadosomatic index, seminiferous tubular (ST) diameter, and ST volumetric proportion, and increased the volumetric proportion of interstitium after 56 days. In the epididymis caput, the tubular volumetric proportion decreased along with an increase of interstitial volumetric proportion and epithelium height after 56 days. The alterations observed were less severe only after 7 days. A progressive testicular damage resulted mainly in tubules lined only by Sertoli cells. The sperm number and cell debris decreased in the epididymis. We demonstrated that the testicular damage induced by single acute i.p. exposure to Cd occurred despite the daily oral intake of AL extract.
Assuntos
Arctium/química , Cádmio/toxicidade , Extratos Vegetais/farmacologia , Testículo/metabolismo , Animais , Epididimo/metabolismo , Epididimo/patologia , Masculino , Extratos Vegetais/química , Ratos , Ratos Wistar , Testículo/patologiaRESUMO
Leaves of Arctium lappa contain several mono- and dicaffeoylquinic acids, as evaluated by liquid chromatography-mass spectrometry. In order to investigate the protection on gastric mucosa against ulcers, rats were treated with fractions from leaf extract prior to ethanol-induced ulcers. The original fraction obtained as ethanol soluble fraction from hot aqueous extract was able to protect de gastric mucosa, and this effect was retained in the ethyl acetate fraction, obtained from liquid/liquid fractionation. The main compound in this fraction was isolated and chemically characterized by nuclear magnetic resonance and mass spectrometry, assisted by isopropylidene derivatization which gave rise a mass increment of 40 units. Therefore, the underivatized compound that had m/z 515.119 [M-H](-) was shifted to m/z 555.151, being confirmed as 1,3-O-dicaffeoylquinic acid, which presented an ED50 of 57 µg kg(-1) on gastric protection, lesser than the therapeutic concentration of omeprazole (40 mg kg(-1)).
Assuntos
Antiulcerosos , Arctium , Ácido Quínico/análogos & derivados , Úlcera Gástrica/tratamento farmacológico , Animais , Antiulcerosos/química , Antiulcerosos/isolamento & purificação , Antiulcerosos/uso terapêutico , Etanol , Isomerismo , Extratos Vegetais/química , Folhas de Planta , Ácido Quínico/química , Ácido Quínico/isolamento & purificação , Ácido Quínico/uso terapêutico , Ratos Wistar , Úlcera Gástrica/induzido quimicamenteRESUMO
The objective of this study was to evaluate the effect of aqueous extracts of Baccharis trimera, Plantago sp., Verbena officinalis and Arctium lappa on the M. javanica control in the greenhouse. The experiment was performed in plastic pots containing a soil mixture and sand 2:1 (volume: volume), it was infested with 4.000 eggs of Meloidogyne javanica and 200 mL of the respective aqueous extract was added. After seven days a tomato seedling was transplanted in each pot. The evaluations of the number of galls and eggs of the nematode by root system were performed after 50 days of transplanting the seedlings. The data was subjected to analysis of variance and the means were compared by Duncan test at 5% probability. None of the tested treatments reduced the number of galls and eggs of M. javanica per root system.(AU)
O objetivo desse ensaio foi avaliar o efeito de extratos aquosos de Baccharis trimera, Plantago sp., Verbena officinalis e Arctium lappa no controle de Meloidogyne javanica, em casa de vegetação. O experimento foi montado em vasos plásticos contendo uma mistura solo e areia 2:1 (volume : volume), infestada com 4.000 ovos de M. javanica e adicionado 200 mL dos respectivos extratos. Após sete dias foi transplantada uma muda de tomate em cada vaso. As avaliações do número de galhas e de ovos do nematoide por sistema radicular foram realizadas após 50 dias do transplantio das mudas. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Duncan, a 5 % de probabilidade. Nenhum dos tratamentos testados reduziu o número de galhas e de ovos de M. javanica por sistema radicular.(AU)
Assuntos
Tylenchoidea/parasitologia , Tratamento do Solo/análise , Baccharis , VerbenaRESUMO
The objective of this study was to evaluate the effect of aqueous extracts of Baccharis trimera, Plantago sp., Verbena officinalis and Arctium lappa on the M. javanica control in the greenhouse. The experiment was performed in plastic pots containing a soil mixture and sand 2:1 (volume: volume), it was infested with 4.000 eggs of Meloidogyne javanica and 200 mL of the respective aqueous extract was added. After seven days a tomato seedling was transplanted in each pot. The evaluations of the number of galls and eggs of the nematode by root system were performed after 50 days of transplanting the seedlings. The data was subjected to analysis of variance and the means were compared by Duncan test at 5% probability. None of the tested treatments reduced the number of galls and eggs of M. javanica per root system.
O objetivo desse ensaio foi avaliar o efeito de extratos aquosos de Baccharis trimera, Plantago sp., Verbena officinalis e Arctium lappa no controle de Meloidogyne javanica, em casa de vegetação. O experimento foi montado em vasos plásticos contendo uma mistura solo e areia 2:1 (volume : volume), infestada com 4.000 ovos de M. javanica e adicionado 200 mL dos respectivos extratos. Após sete dias foi transplantada uma muda de tomate em cada vaso. As avaliações do número de galhas e de ovos do nematoide por sistema radicular foram realizadas após 50 dias do transplantio das mudas. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Duncan, a 5 % de probabilidade. Nenhum dos tratamentos testados reduziu o número de galhas e de ovos de M. javanica por sistema radicular.
Assuntos
Baccharis , Tratamento do Solo/análise , Tylenchoidea/parasitologia , VerbenaRESUMO
A candidíase vaginal é uma doença causada, na maioria das vezes, pelo fungo do gênero Candida sp, que habita o trato gastrintestinal e geniturinário da espécie humana e pode tornar-se patogênico sob determinadas condições. A maioria dos indivíduos desenvolve defesas imunológicas que impedem a proliferação e desenvolvimento de candidíase localizada ou disseminada. Embora a causa exata do aumento de espécies não-albicans seja desconhecida, há evidências de que a própria terapia antifúngica possa estar contribuindo para o processo. Linhagens de C. glabrata são mais resistentes aos imidazólicos do que a C. albicans, sendo necessária uma concentração 10 vezes superior de miconazol para eliminar a C. glabrata quando comparada a C. albicans. Foi realizado um antifungigrama testando o potencial de ação de produtos vegetais sobre o fungo Candida sp. Foi observado que, ocorreu inibição do fungo no contato com os extratos hidroglicólicos das plantas Arctium lappa L., Calendula officinalis L., Stryphnodendron adstringens (Mart.) Coville e Tabebuia avellanedae Lorentz ex Griseb. A importância deste trabalho reside na possibilidade de desenvolvimento de tratamento complementar, menos agressivo, de menor custo e sem toxidade, o que possibilitaria melhor qualidade de vida para portadoras de candidíase vaginal recorrente ou não.
Vaginal candidiasis is a disease caused, in most cases, by the fungus of the genus Candida sp., which inhabits the gastrointestinal and genitourinary tracts of the human species and can become pathogenic under certain conditions. Most individuals develop immune defenses that prevent the proliferation and the development of localized or disseminated candidiasis. Although the exact cause of the increase in non-albicans species is unknown, there is evidence that antifungal therapy itself may have contributed to it. Strains of C. glabrata are more resistant to imidazole than C. albicans, and a 10-fold higher concentration of miconazole is required to eliminate C. glabrata compared to C. albicans. Etest was performed by testing the action potential of plant products on the fungus Candida sp. Inhibition of the fungus occurred when it was in contact with the hydroglycol extracts of plants Arctium lappa L., Calendula officinalis L., Stryphnodendron adstringens (Mart.) Coville and Tabebuia avellanedae Lorentz ex Griseb. The importance of this study lies in the possibility of developing a complementary treatment, less aggressive, of lower cost and without toxicity, which would allow better life quality for women with vaginal candidiases that are recurrent or not.
Assuntos
Candidíase/tratamento farmacológico , Extratos Vegetais/uso terapêutico , Calendula/metabolismo , Tabebuia/metabolismo , Arctium/metabolismo , Fabaceae/metabolismo , Micoses/prevenção & controle , Antifúngicos/análiseRESUMO
Conduziu-se este experimento, com o objetivo de avaliar o efeito da incorporação ao solo de fósforo (P) e de cama-de-frango (CF) sobre o crescimento e a produção da bardana. Estudaram-se cinco doses de P (4,3; 25,8; 43,0; 60,2 e 81,7 kg ha-1) e cinco doses de CF (1; 6; 10; 14 e 19 t ha-1) perfazendo nove tratamentos, definidos por meio da matriz experimental PLAN PUEBLA III, dispostos no delineamento experimental blocos casualizados, com quatro repetições. A altura máxima (129,87 cm) foi alcançada aos 113 dias após o transplante, com o tratamento de 60,2 e 14 t ha-1 de P e CF, respectivamente. As maiores produções de massa fresca das folhas foram de 22,87 Mg ha-1, quando se utilizou 81,7 kg ha-1 de P e de 18,38 Mg ha-1 com a dose de 19 t ha-1 de CF. As maiores produções de massa seca das folhas ocorreram sob as maiores doses de CF. As maiores produções de massas frescas de raízes foram de 2,44 Mg ha-1 e 2,00 Mg ha-1, alcançadas com os tratamentos 81,7 kg ha-1 de P e 1 t ha-1 de CF e 4,3 kg ha-1 de P e 19 t ha-1 de CF, respectivamente. A maior produção de massa seca de raiz foi 0,33 Mg ha-1 obtida com o uso das maiores doses de fósforo e doses intermediárias de CF.
The aim of this study was to evaluate the effect of incorporating phosphorus (P) and chicken manure (CM) to the soil on the growth and yield of burdock. Five doses of phosphorus (4.3, 25.8, 43.0, 60.2, and 81.7 kg ha-1) in triple super phosphate (TSP) form and five doses of semi-decomposed chicken manure (1, 6, 10, 14, and 19 t ha-1) were studied. Nine treatments were defined using Plan Puebla III experimental matrix. Treatments were arranged in a randomized block experimental design with four replications. Maximum height of burdock (129.87 cm) was reached on the 113th day after transplant (DAT) with 60.2 and 14 t ha-1 of phosphorus and chicken manure treatments, respectively. The greatest yields of leaf fresh matter were 22.87 Mg ha-1 when 81.7 kg ha-1 P was used and 18.38 Mg ha-1 with dose of 19 t ha-1 CM. The highest yields of leaf dry matter were related to the highest doses of chicken manure. The greatest yields of root fresh matter were 2.44 Mg ha-1 and 2.00 Mg ha-1, which were reached with treatments of 81.7 kg ha-1 P and 1 t ha-1 CM and 4.3 kg ha-1 P and 19 t ha-1 CM, respectively. The highest yield of root dry matter was 0.33 Mg ha-1 obtained with the greatest doses of P and intermediary doses of CM.
RESUMO
This study was carried out to evaluate the effects of Arctium lappa L. (burdock) on the liver of adult male Wistar rats as measured by light microscopy and biochemical parameters. The rats received the extract in water bottles at doses of 10 or 20 g/L daily for 40 days. There were no significant changes in the plasma levels of albumin, aspartate transaminase (AST), alanine transaminase (ALT), gamma glutamyl transferase (GGT), total protein, total cholesterol, urea, uric acid, triacylglycerol, calcium, phosphorus, chlorine and direct bilirubin. The morphological analysis did not reveal histopathological alterations in liver tissue. Both biochemical and morphological data did not indicate A. lappa toxicity.
A bardana (Arctium lappa L) é uma planta trazida do Japão e aclimatada no Brasil, e é extensamente utilizada na medicina popular em todo mundo. Este estudo foi realizado para avaliar os possíveis efeitos da A. lappa no fígado e nos parâmetros bioquímicos plasmáticos em ratos Wistar adultos. Estes receberam a infusão de bardana nas doses de 10 ou 20 g de folhas secas /L de água, por 40 dias. Não houve alteração significativa nos níveis plasmáticos de albumina, aspartato transaminase (AST), alanina transaminase (ALT), gamma glutamil transferase (GGT), proteínas totais, colesterol total, uréia, ácido úrico, triglicérides, cálcio, fósforo, bilirrubina direta e cloro. A análise morfológica não revelou alterações histopatológicas no fígado. Os dados bioquímicos e morfológicos não indicaram a toxicidade da bardana.
RESUMO
Angiostrongylus costaricensis é um parasita que causa angiostrongilíase abdominal em humanos, seu tratamento inclui o uso de antiinflamatórios apesar da falta de estudos que justifiquem esta conduta. O objetivo deste artigo é avaliar o efeito da betametasona e da Arctium lappa na evolução de lesões intestinais induzidas pelo parasita. Utilizou-se camundongos Swiss, machos, adultos, distribuídos em 4 grupos: infectados tratados com betametasona; com Arctium lappa; não tratados e grupo controle. Os tratamentos iniciaram no 15º dia de infecção e permaneceram por 15 dias. Infiltrado eosinofílico e granuloma foram avaliados (1-leve; 2-moderado; 3-severo). A betametasona permitiu a evolução das lesões para formas mais graves, enquanto o extrato não interferiu na progressão da patologia. As substâncias empregadas não mostraram eficácia na proteção das lesões induzidas pelo Angiostrongylus costaricensis em camundongos. Estes achados desmotivam o uso de betametasona e Arctium lappa em humanos acometidos por angiostrongilíase abdominal.
Angiostrongylus costaricensis is a parasite that causes abdominal angiostrongyliasis in humans. The treatment for it includes the use of anti-inflammatory drugs, despite the lack of studies to justify this approach. The objective of this paper was to evaluate the effect of betamethasone and Arctium lappa on the evolution of intestinal lesions induced by this parasite. Adult male Swiss mice were used, distributed into four groups: infected and treated with betamethasone; infected and treated with Arctium lappa; infected and not treated; and control group. The treatments were started on the 15th day after infection and continued for 15 days. The presence of eosinophilic infiltration and granuloma was evaluated (1-mild; 2-moderate; 3-severe). Betamethasone allowed the lesions to evolve into more severe forms, while the extract did not interfere with disease progression. The substances applied were ineffective for protection against the lesions induced by Angiostrongylus costaricensis in mice. These findings discourage the use of betamethasone and Arctium lappa for humans affected by abdominal angiostrongyliasis.
Assuntos
Animais , Masculino , Camundongos , Anti-Inflamatórios/uso terapêutico , Arctium/química , Betametasona/uso terapêutico , Extratos Vegetais/uso terapêutico , Infecções por Strongylida/tratamento farmacológico , Modelos Animais de Doenças , Índice de Gravidade de DoençaRESUMO
Arctium lappa (burdock) has been used to treat inflammatory processes. Blood constituents labeled with technetium-99m (99mTc) have been utilized in nuclear medicine. It was evaluated the influence of a burdock extract on the labeling of blood constituents with 99mTc and on the morphometry of red blood cells. Blood samples from Wistar rats were incubated with burdock extract and the radiolabeling procedure was carried out. Plasma and blood cells, soluble and insoluble fractions of plasma and blood cells were separated. The radioactivity in each fraction was counted and the percentages of radioactivity ( percentATI) were determined. Morphology and morphometric (perimeter/area ratio) measurements of red blood cells (RBC) were performed. The incubation with burdock extract significantly (p<0.05) altered the percentATI on the blood compartments and the perimeter/area ratio of RBC, as well as, induced modifications on the shape of RBC. Alterations on membrane could justify the decrease of labeling of blood cells with 99mTc obtained in this study.
Arctium lappa (bardana) tem sido utilizada na medicina popular para o tratamento de processos inflamatórios. Constituintes sangüíneos marcados com tecnécio-99m (99mTc) são utilizados na medicina nuclear para obtenção de imagens. Neste trabalho foi avaliada a influência de um extrato de bardana na marcação de constituintes sangüíneos com 99mTc e na morfologia de hemácias. Amostras de sangue de ratos Wistar foram incubadas com extrato de bardana e o processo de radiomarcação de constituintes sangüíneos foi realizado. Plasma e células sangüíneas, frações solúvel e insolúvel do plasma e das células sangüíneas foram separadas, a radioatividade em cada fração foi contada e as porcentagens de radioatividade ( por centoATI) foram determinadas. A morfologia e a relação perímetro/área das hemácias foram avaliadas. A incubação de sangue com o extrato de bardana alterou significativamente (p<0.05) a por centoATI a distribuição de radioatividade nos compartimentos plasmático e celular. A relação perímetro/área de hemácias, bem como a forma das hemácias também sofreram alterações Modificações na membrana poderiam justificar a diminuição da marcação das células sangüíneas com 99mTc obtidas neste estudo.