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The 7-ethoxyresorufin-O-deethylase (EROD) activity was first time characterized in the neotropical fish Cnesterodon decemmaculatus as a biomarker for assessing environmental health in aquatic ecosystems of the Rio de la Plata Basin impacted by organic pollutants agonist of the aryl-hydrocarbon receptor (AhR). Both laboratory and field studies were conducted. Laboratory experiments were run using ß-naphthoflavone (BNF) as an AhR agonist model. A clear concentration-response relationship was found between 1 and 100 µg/L, with a NOEC and LOEC of 1 and 10 µg/L. A fast time-dependent response was observed with a significant induction after 24 h and a plateau from 24 to 48 h up to 264 h of exposure. Differences in basal activity were found between juveniles, females, and males, but induction levels were similar. Both basal activities and induction levels were distinct in the whole body, liver, gill, muscle, brain, and embryos. Fold-change inductions in the respective tissues were: 20, 114, 3, 5, 1, and 14. Maternal transfer and early cyp1a activation were unveiled by embryonic induction. Clear differences in EROD activity were found among juveniles collected in hydrocarbon-polluted streams, beside the La Plata Petrochemical hub, and a reference stream. Similar EROD activities were observed in laboratory and feral fish, usually with values below or above 1,000 pmol/min x mg protein for unexposed or exposed organisms. The study contributes with original information about EROD activity in C. decemmaculatus that encourages the use of both the response as a robust biomarker of exposure and the species as a good sentinel organism to be included in surveillant programs for assessing aquatic pollution by AhR agonist chemicals within the Rio de la Plata Basin within the One Health paradigm.

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Coastal environments, such as those in the Santa Catarina State (SC, Brazil), are considered the primary receptors of anthropogenic pollutants. In this study, our objective was to evaluate the levels of emerging contaminants (ECs) and persistent organic pollutants (POPs) in indigenous Crassostrea gasar oysters from different regions of SC coast in the summer season (March 2022). Field collections were conducted in the São Francisco do Sul, Itajaí, Florianópolis and Laguna coastal zones. We analyzed the bioaccumulation levels of 75 compounds, including antibiotics (AB), endocrine disruptors (ED), non-steroidal anti-inflammatory drugs (NSAIDs), polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and pesticides. Furthermore, we assessed biomarker responses related to biotransformation, antioxidant defense, heat shock protection and oxidative damage in oysters' gills. Prevalence of ECs was observed in the central and southern regions, while the highest concentrations of POPs were detected in the central-northern regions of SC. Oysters exhibited an induction in biotransformation systems (cyp2au1 and cyp356a1, sult and GST activity) and antioxidant enzymes activities (SOD, CAT and GPx). Higher susceptibility to lipid peroxidation was observed in the animals from Florianópolis compared to other regions. Correlation analyses indicated possible associations between contaminants and environmental variables in the biomarker responses, serving as a warning related to climate change. Our results highlight the influence of anthropogenic activities on SC, serving as baseline of ECs and POPs levels in the coastal areas of Santa Catarina, indicating more critical zones for extensive monitoring, aiming to conserve coastal regions.

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Due to the increasing use and inadequate disposal of plastic by humans, aquatic environments have become receptacles for pollutants such as plastic. This study aimed to perform an analysis of plastic particles pollution in Brazilian aquatic ecosystems with special attention to inland aquatic environments and fish in order to identify information gaps in this field. Manuscripts published in the last 21 years and indexed in the Web of Science database were consulted. A total of 185 met the proposed inclusion criteria, such as having empirical data, being conducted in Brazil, and dealing with plastic pollution. In general, the number of studies increases over the years, and this increasing number of publications is accompanied by declared financial support; the Southeast and Northeast regions are the regions that publish the most on the topic, with São Paulo, Rio de Janeiro, and Pernambuco being the main states; the main focus of the studies is the detection of plastic particles mainly in biota (51%) and sediment (34%), and the most frequent ecosystem is the marine (89%); regarding the taxa, the majority is about plastic detection in fish (75%). Only 18% of the papers studying fishes consider their bio-ecological data, and only 17% of the manuscripts carried out the chemical characterization of the particles. However, 99% of the papers considered the shape of the plastic particle. We emphasize the need for more research and grants for studies with Brazilian inland aquatic ecosystems on the effects of plastic particle pollution on freshwater fish. Regional and national research funding agencies are very important to encourage an increase in the number of grants and specific calls for studies on plastic pollution and its impact on freshwater biota, considering the different macro-regions in Brazil, especially in the northern region.

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Abstract Environmental contamination with heavy metals is a threat to the organisms due to their toxicity, persistence and bioaccumulation in food chains. The study was aimed to assess cadmium (Cd) effect on calcium (Ca) level in bones of a freshwater fish Channa gachua. 42 fish individuals were kept into six (6) aquaria; labelled aq.0, 1, 2, 3,4 and 5 in the laboratory for treatment. Aq.0 was control group and aq.1, 2,3,4,5 were experimental with treatment solution of Cd 0, 0.1ppm, 0.5ppm, 1ppm, 2.5ppm and 5ppm respectively for three months. After exposure, bones tissue were examined for Cd accumulation and Ca concentration. Highest accumulation of Cd were recorded in aq.5 mean 46.86 ± 0.46 mgkg-1 .and lowest in the control group with mean 0.61 ± 0.06 mgkg-1. The order of Cd bioaccumulation in bones were aq.5 > aq.4 >aq.3 > aq.2 > aq.1 > aq.0. Highest concentration of Ca were noted in aq.0 (Control group) mean 7888.06 ± 4827.22 mgkg-1 and lowest were 1132.36 ± 203.73 mgkg-1 in aq.5 (at 5.0 ppm). Generally a pattern of decreasing Ca level were observed with each rise of Cd bioaccumulation aq.0 > aq.1 > aq.2 > aq.3 > aq.4 > aq.5. Current study indicated that Cd accumulation have substantial effect on Ca level in bones and hence on skeleton system. Strict rules must be implemented by government to control metals pollution and exploitations of biota.

A contaminação ambiental com metais pesados ​​é uma ameaça aos organismos devido à sua toxicidade, persistência e bioacumulação nas cadeias alimentares. O estudo teve como objetivo avaliar o efeito do Cd sobre o nível de Ca em ossos de peixes de água doce Channa gachua. Quarenta e dois indivíduos de peixes foram mantidos em seis aquários; marcado aq. 0, 1, 2, 3, 4 e 5 no laboratório para tratamento. Aq. 0 foi o grupo controle e aq. 1, 2, 3, 4, 5 foram experimentais com solução de tratamento de Cd 0, 0,1 ppm, 0,5 ppm, 1 ppm, 2,5 ppm e 5 ppm respectivamente por três meses. Após a exposição, o tecido ósseo foi examinado quanto ao acúmulo de Cd e concentração de Ca. O maior acúmulo de Cd foi registrado em aq. 5 com média de 46,86 ± 0,46 mgkg-1 e o menor no grupo controle com média de 0,61 ± 0,06 mgkg-1. A ordem de bioacumulação de Cd nos ossos foi aq. 5 > aq. 4 > aq. 3 > aq. 2 > aq. 1 > aq. 0. A concentração mais alta de Ca foi observada em aq. 0 (grupo controle) média de 7.888,06 ± 4.827,22 mgkg-1 e a mais baixa foi de 1.132,36 ± 203,73 mgkg-1 em aq. 5 (a 5,0 ppm). Geralmente um padrão de diminuição do nível de Ca foi observado com cada aumento da bioacumulação de Cd aq. 0 > aq. 1 > aq. 2 > aq. 3 > aq. 4 > aq. 5. O estudo atual indicou que o acúmulo de Cd tem efeito substancial no nível de Ca nos ossos e, portanto, no sistema esquelético. Regras estritas devem ser implementadas pelo governo para controlar a poluição por metais e a exploração da biota.

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Brazil has abundant surface water resources, huge aquatic biodiversity and is home to 213 million people. Genotoxicity assays are sensitive tools to detect the effects of contaminants in surface waters and wastewaters, as well as to determine potential risks of contaminated waters to aquatic organisms and human health. This work aimed to survey the articles published in 2000-2021 that evaluated the genotoxicity of surface waters within Brazilian territory to unveil the profile and trends of this topic over time. In our searches, we considered articles focused on assessing aquatic biota, articles that conducted experiments with caged organisms or standardized tests in the aquatic sites, as well as articles that transported water or sediment samples from aquatic sites to the laboratory, where exposures were performed with organisms or standardized tests. We retrieved geographical information on the aquatic sites evaluated, the genotoxicity assays used, the percentage of genotoxicity detected, and, when possible, the causative agent of aquatic pollution. A total of 248 articles were identified. There was a trend of increase in the number of publications and annual diversity of hydrographic regions evaluated over time. Most articles focused on rivers from large metropolises. A very low number of articles were conducted on coastal and marine ecosystems. Water genotoxicity was detected in most articles, regardless of methodological approach, even in little-studied hydrographic regions. The micronucleus test and the alkaline comet assay were widely applied with blood samples, mainly derived from fish. Allium and Salmonella tests were the most frequently used standard protocols. Despite most articles did not confirm polluting sources and genotoxic agents, the detection of genotoxicity provides useful information for the management of water pollution. We discuss key points to be assessed to reach a more complete picture of the genotoxicity of surface waters in Brazil.

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Although carbon-based nanomaterials (CNMs) toxicity has already been demonstrated in some animal models, little is known about the impact of carbon nanofibers (CNFs) on aquatic vertebrates. Thus, we aimed to evaluate the possible effects of long-term exposure of zebrafish (Danio rerio) juveniles (90 days) to CNFs in predicted environmentally relevant concentrations (10 ng/L and 10 µg/L). Our data revealed that exposure to CNFs did not affect the growth and development of the animals, in addition to not having induced locomotor alterations or anxiety-like behavior. On the other hand, we observed that zebrafish exposed to CNFs showed a response deficit to the vibratory stimulus test, alteration in the density of neuromasts recorded in the final ventral region, as well as an increase in thiobarbituric acid reactive substances levels and a reduction in total antioxidant activity, nitric oxide, and acetylcholinesterase activity in the brain. Such data were directly associated with a higher concentration of total organic carbon in the brain, which suggests the bioaccumulation of CNFs. Furthermore, exposure to CNFs induced a picture suggestive of genomic instability, inferred by the increased frequency of nuclear abnormalities and DNA damage in circulating erythrocytes. Although the individual analyses of the biomarkers did not point to a concentration-dependent effect, the principal component analysis (PCA) and the Integrated Biomarker Response Index (IBRv2) indicate a more prominent effect induced by the higher CNFs concentration (10 µg/L). Therefore, our study confirms the impact of CNFs in the studied model (D. rerio) and sheds light on the ecotoxicological risks of these nanomaterials to freshwater fish. Based on the ecotoxicological screening provided by our study, new horizons are opened for investigations into the mechanisms of action of CNFs, which will help understand the magnitude of the impact of these materials on aquatic biota.

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Some studies have shown that freshwater ecosystems are polluted in a similar proportion to marine ecosystems; however, there are many gaps to be filled in this topic. Here, we investigated whether plastics were consumed by carnivore fishes in a Neotropical floodplain and whether it was connected to seasonality (dry and wet seasons). We also evaluated the association between each type of plastic and the fish species. We analyzed the gastrointestinal contents of 23 species and assessed the occurrence and number of plastic particles. Plastics were obtained through chemical digestion and the spectrum of each sample, using a FT-IR imaging microscope. We performed a correspondence analysis (CA) with plastic data to assess the relationship between each type of plastic and the fish species. We also performed linear regression models to assess the relationships of occurrence and number of plastics ingested with seasonality. Nine species had plastics in their gastrointestinal contents, and they were identified as polyvinyl alcohol (PVA), polyamide (PA), polyethylene (PE), polystyrene (PS), and polypropylene (PP). The number of plastics had a positive relationship with the wet season, while the occurrence did not show a significant relationship with any season. These results are particularly important when considering the socioeconomic relevance and the ecological importance of this trophic guild.

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Studies in recent years have shown that aquatic pollution by microplastics (MPs) can be considered to pose additional stress to amphibian populations. However, our knowledge of how MPs affect amphibians is very rudimentary, and even more limited is our understanding of their effects in combination with other emerging pollutants. Thus, we aimed to evaluate the possible toxicity of polyethylene MPs (PE-MPs) (alone or in combination with a mix of pollutants) on the health of Physalaemus cuvieri tadpoles. After 30 days of exposure, multiple biomarkers were measured, including morphological, biometric, and developmental indices, behavioral parameters, mutagenicity, cytotoxicity, antioxidant and cholinesterase responses, as well as the uptake and accumulation of PE-MPs in animals. Based on the results, there was no significant change in any of the parameters measured in tadpoles exposed to treatments, but induced stress was observed in tadpoles exposed to PE-MPs combined with the mixture of pollutants, reflecting significant changes in physiological and biochemical responses. Through principal component analysis (PCA) and integrated biomarker response (IBR) assessment, effects induced by pollutants in each test group were distinguished, confirming that the exposure of P. cuvieri tadpoles to the PE-MPs in combination with a mix of emerging pollutants induces an enhanced stress response, although the uptake and accumulation of PE-MPs in these animals was reduced. Thus, our study provides new insight into the danger to amphibians of MPs coexisting with other pollutants in aquatic environments.

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Microplastic contamination is a global concern due to its conspicuous presence in aquatic ecosystems and its toxic nature to environmental and human health. False mussels are among the most notable fresh- and brackish water invaders. The invasive Mytilopsis leucophaeata in Rodrigo de Freitas Lagoon-RFL (Rio de Janeiro, Brazil) is the most abundant macrofaunal invertebrate, widely established and distributed throughout the lagoon. This study aimed to assess microplastic contamination in this invasive filter feeder and evaluate its potential use as a bioindicator. Agglomerates (~100 mussels) were manually collected using a stainless-steel spatula in ten sampling areas distributed throughout the whole lagoon and kept frozen. In the laboratory, 60 individuals were sorted by area for soft-tissue digestion. Each pool of 10 soft-tissue mussels (n = 6 by area) was wet-weighted and then placed in a 150-mL decontaminated glass beaker with 50 mL of 10% KOH. Samples were heated (40 °C) for 48 h, and digested samples were filtered in glass-fiber membranes. Microplastics were found in all samples of mussels (n = 60) from RFL; the particles were mostly lower than 100 µm with a mean concentration (±SD) of 35.96 ± 47.64 MPs g wet-weight-1. Microplastics were distinguished in seven shapes with different occurrences in samples (%): fiber (43.3%); fragment (34.3%); film (16.3%); sponge/foam (4.9%); pellet (0.57%), rope/filaments (0.17%); and undefined (0.4%). Thirteen colors of microplastics were found, but transparent (54.94%), black (10.77%), and white (9.36%) were the most common. Mytilopsis leucophaeata were useful to assess microplastic contamination in RFL and might be preferentially used in other invaded brackish systems instead of native and often threatened bivalves. Our results confirm the effective application of bivalves as an indicator of coastal microplastic pollution.

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Abstract The great biodiversity of neotropical fish species that have external fertilization as a reproductive strategy, like the tambaqui, requires more careful analyzes in toxicological tests of the various pesticides implemented in Brazilian agriculture over the last few years. In this context, the objective of the present work was to evaluate possible sperm alterations in tambaqui (Colossoma macropomum) semen exposed to two different pesticide residues. Seminal samples of sexually mature tambaqui males from a local fish farm were used. Semen was collected eight hours after hormone induction into graduated glass tubes. After initial assessment of the lack of prior activation, the experiment was carried out in a factorial scheme, testing two pesticides widely used in agricultural systems (glyphosate and fenitrothion). For each pesticide, five concentrations were tested (6, 12, 24, 120 and 240 mg/L), with motility analysis at times 0, 30 and 60 seconds after activation. As a control, activation with 0.9% NaCl solution and motility analysis at the same times described for pesticides were used. Results indicate that in natura samples exhibited initial motility of 89.2 ± 4.9% and mean duration of 100 seconds (up to 10% sperm motility). The reduction in sperm motility occurred significantly (p < 0.05) after 30 seconds in all concentrations tested, except for the concentration of 240 mg/L because no activation was observed. The tests described here demonstrate that tambaqui semen was sensitive to the process of exposure to pesticide residues, and can be used in biomonitoring analyzes of the aforementioned agricultural pesticides.

Resumo A grande biodiversidade das espécies de peixes neotropicais que possuem a fertilização externa como estratégia reprodutiva, a exemplo do tambaqui, exige análises mais criteriosas em testes toxicológicos dos diversos defensivos agrícolas implementados na agricultura brasileira ao longo dos últimos anos. Nesse contexto, o objetivo do presente trabalho foi avaliar possíveis alterações espermáticas no sêmen de tambaqui (Colossoma macropomum) exposto a resíduos de dois diferentes pesticidas. Foram utilizadas amostras seminais de machos de tambaqui sexualmente maduros provenientes de uma piscicultura local. O sêmen foi coletado oito horas pós indução hormonal em tubos de vidro graduados. Após avaliação inicial de inexistência de ativação prévia, foi realizado o experimento em esquema fatorial, sendo testados dois pesticidas muito utilizados em sistemas agrícolas (glifosato e fenitrotiona). Para cada pesticida foram testadas cinco concentrações (6, 12, 24, 120 e 240 mg/L), com análise da motilidade nos tempos 0, 30 e 60 segundos pós ativação. Como controle, foi utilizada a ativação com solução de NaCl a 0,9% e análise da motilidade nos mesmos tempos descritos para os pesticidas. Resultados indicam que as amostras in natura exibiram motilidade inicial de 89,2 ± 4,9% e tempo de duração médio de 100 segundos (até 10% de motilidade espermática). A redução da motilidade espermática ocorreu de forma significativa (p < 0,05) após 30 segundos em todas as concentrações testadas, exceto na concentração de 240 mg/L por não ter sido observada ativação. Os testes aqui descritos demonstram que o sêmen de tambaqui se mostrou sensível ao processo de exposição aos resíduos de pesticidas, podendo ser utilizado em análises de biomonitoramento dos referidos defensivos agrícolas.

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Response of biomarkers to chemical contamination was evaluated in crabs of the Callinectes genus (Callinectes ornatus and C. bocourti) from two tropical estuaries (São José and São Marcos bays) of the Maranhão State (northeastern Brazil). Biomarkers evaluated included hepatopancreatic metallothionein-like proteins (MTLP) and lipid peroxidation (LPO), as well as muscle acetylcholinesterase (AChE). Tissue concentrations of metals (pereiopod muscle and hepatopancreas), hydrocarbons (hepatopancreas) and organochlorine pesticides (hepatopancreas) were also evaluated. Crab samples were collected in three sites of each estuary (São Marcos Bay and São José Bay). Sampling was performed in August/2012 (dry season), January/2013 (rainy season), August/2013 (dry season), and January/2014 (rainy season). Concentrations of chemical contaminants and responses of biomarkers showed significant spatial (São Marcos Bay and São José Bay) and/or seasonal (dry and rainy seasons) and annual (2012-2014) variability. However, a general higher Zn concentration was observed in hepatopancreas of crabs from São José Bay. In turn, a general higher Cd concentration paralleled by oxidative damage (LPO) was observed in hepatopancreas of crabs from São Marcos Bay. As expected, these findings support the idea that this bay is more intensively or chronically impacted by industrial activities while the São José Bay is likely more affected by domestic activities. Interestingly, LPO level in crab hepatopancreas showed to be the most reliable and adequate biomarker to distinguish the two bays.

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The aim of this study was to determine selected molecular biomarkers of iron ore contamination in Epinephelus marginatus. Molecular biomarkers were tested by checking the relative expression of genes involved in oxidative stress, trace element regulation, and cellular damage, by RT-qPCR. Iron ore exposure caused the downregulation of ferroportin (FP) gene expression and a significant upregulation in superoxide dismutase (SOD) and cytochrome P450 1A (CYP1A) genes. Iron ore affects gene expression in E. marginatus liver, indicating that it can potentially induce toxic effects in fish. Moreover, this altered gene expression pattern may be applied in monitoring iron ore contamination in marine environments.

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The use of herbicides has increased over the last decades. Glyphosate is the most widely used herbicide commercialized in more than 750 formulations. While information about glyphosate's toxicity on different non-target aquatic organisms has been vastly documented, we know little about the transgenerational effects in aquatic biota. This study determined the cross-generation effects produced by the glyphosate-based herbicide Faena® on the American cladoceran Daphnia exilis. Measured endpoints were survival, reproductive responses, metabolic biomarkers, and the size of neonates. D. exilis was exposed to glyphosate concentrations of 2.09, 2.49, and 3.15 (mg L-1) (as content in Faena®) during 21 days starting from neonates, at 25°C, 16:8 photoperiod, fed with 8 × 105 cells mL-1 of Pseudokirchneriella subcapitata. The LC50 was 4.22 mg L-1. Survival, accumulated progeny, and the number of clutches in the parental generation (P1) were significantly higher than those observed in the first generation (F1). Exposure to the herbicide completely inhibited reproduction in the F1. The size of the neonates varied among treatments and broods in P1; nevertheless, neonate size (body and total lengths, as well as body width) was significantly affected in F1. Toxic effects on the survival and reproduction of D. exilis were significantly increased in the F1 exposed to Faena®. Results warn about the augmented effect on progeny where parents were exposed to this herbicide. Multigenerational adverse effects could be expected in freshwater zooplankton exposed to Faena®. The frequently claimed low toxicity of glyphosate must be revised to control the indiscriminate use of this herbicide.

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Caribbean islands, including Puerto Rico, are biodiversity hotspots threatened by microplastics (<5 mm). Little is known about the extent of microplastic pollution in coastal sandy beaches of Puerto Rico. Sand from six northern beaches was collected in the high tide line to determine microplastic abundance (0.3-4.75 mm). Península La Esperanza, the most polluted beach, exhibited higher average abundance (17 items/kg dw) and diversity. High urbanization, industrial/port activities, and riverine input are likely sources of plastic debris on this beach. The other beaches showed lower and similar average abundance (3 to 7 items/kg dw) despite having distinct potential point and non-point sources. Overall, fibers (40%), fragments (28%) and foams (27%) predominated (n = 102 particles). Results showed comparable levels to other world beaches, some classified as highly contaminated, but only when transforming units to items/m2. Preliminary ATR-FTIR analysis identified mainly polyethylene. It is imperative to have plastics source reduction through waste management.

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Natural and synthetic steroid hormones, excreted by humans and farmed animals, have been considered as important sources of environmental endocrine disruptors. A suite of estrogens, androgens and progestogens was measured in the wastewater treatment plant outfall (WWTPO) of Chascomús city (Buenos Aires province, Argentina), and receiving waters located downstream and upstream from the WWTPO, using solid phase extraction and high-performance liquid chromatography mass spectrometry. The following natural hormones were measured: 17ß-estradiol (E2), estrone (E1), estriol (E3), testosterone (T), 5α-dihydrotestosterone (DHT), progesterone (P), 17-hydroxyprogesterone (17OHP) and the synthetic estrogen 17α-ethinylestradiol (EE2). Also, in order to complement the analytical method, the estrogenic activity in these surface water samples was evaluated using the in vitro transactivation bioassay that measures the estrogen receptor (ER) activity using mammalian cells. All-natural steroid hormones measured, except 17OHP, were detected in all analyzed water samples. E3, E1, EE2 and DHT were the most abundant and frequently detected. Downstream of the WWTPO, the concentration levels of all compounds decreased reaching low levels at 4500 m from the WWTPO. Upstream, 1500 m from the WWTPO, six out of eight steroid hormones analyzed were detected: DHT, T, P, 17OHP, E3 and E2. Moreover, water samples from the WWTPO and 200 m downstream from it showed estrogenic activity exceeding that of the EC50 of the E2 standard curve. In sum, this work demonstrates the presence of sex steroid hormones and estrogenic activity, as measured by an in vitro assay, in superficial waters of the Pampas region. It also suggests the possibility of an unidentified source upstream of the wastewater outfall.

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Anthropogenic activities can have a great influence on water quality and in the availability of habitat and food resources, which can promote changes in the trophic diversity and carbon sources sustaining aquatic communities. The objective of this study was to evaluate if the trophic diversity and the main carbon sources sustaining fish communities change along a pollution gradient. The study was carried out at eight sites distributed along the Rio das Velhas, a Brazilian river highly impacted by anthropogenic activities, in which the discharge of domestic and industrial sewage from the Metropolitan Region of Belo Horizonte (MRBH) presents a major source of pollution. Using carbon (δ13C) and nitrogen (δ15N) isotope ratios, we identified the major carbon sources/food sources of common fish species and calculated six metrics of trophic diversity. Autochthonous primary producers (algae, periphyton, and macrophytes) were the main carbon sources for all trophic guilds at all sites, but notably, sewage-derived organic matter was an additional significant carbon source to the fish community in the most polluted testing site. Here, the community was composed mainly by detritivorous and omnivorous fishes and exhibited greater ranges of carbon and nitrogen isotopic values, large total areas, high trophic diversity, small trophic redundancy, and less even distribution of trophic niches than the less polluted sites. We conclude that the trophic guilds, trophic diversity metrics, and carbon sources sustaining fish communities in the Rio das Velhas are highly influenced by the presence of pollution. Besides favoring omnivorous and detritivorous fishes, the input of sewage also provided an important food source to sustain the fish community from sites close to the MRBH.

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The scientific knowledge about toxicological impacts of polyethylene microplastics (PE MPs) on different organisms has significantly improved in recent years. However, the effects of these pollutants on animal species such as amphibians remain poorly known. Thus, the aim of the current study is to investigate whether the short exposure (7 days) of Physalaemus cuvieri tadpoles to PE MPs (60 mg/L) can change their behavior. Collected data have shown that PE MP accumulation in tadpoles was associated with different behavioral changes observed in them; this outcome has confirmed the behavioral toxicity of these micropollutants in the investigated species. Tadpoles subjected to PE MPs presented locomotion issues, anxiogenic effect symptoms, as well as anti-predatory defensive response deficit when they were exposed to predators (Cyprinus carpio). Data analysis enabled inferring to what extent these pollutants can affect individuals, and their natural predators living in contaminated areas. Based on the biological viewpoint, these changes can affect their defensive response to predators, as well as their social behavior. To the best of our knowledge, the present study was pioneer in reporting PE MPs-induced behavioral toxicity in representatives of amphibian groups.

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Aquatic ecosystems face serious pollution issues. Discharges of toxic substances and eutrophication may lead to changes in the phytoplankton community and foster cyanobacterial blooms. Glyphosate-based herbicides are chemical stressors of microalgae that may affect the structure of phytoplankton communities, and also stimulate the synthesis of cyanotoxins by cyanobacteria. The simultaneous presence of glyphosate and toxigenic cyanobacteria increases the stress on microalgae, jointly affecting their growth and development. This study evaluated the combined effect of a toxigenic cyanobacterium and glyphosate in the development of an experimental microalgal community. We studied the effect of Microcystis aeruginosa on the population growth of the microalgae Ankistrodesmus falcatus, Chlorella vulgaris, Pseudokirchneriella subcapitata, and Scenedesmus incrassatulus. We also evaluated the combined effect of sub-inhibitory glyphosate (Faena®) concentrations on the content of macromolecules and the enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), as well as on the concentration of TBARS. These effects were evaluated through the integrated biomarker response (IBR). In individual experiments, microalgae showed lower growth rates versus M. aeruginosa. In the mixed bioassays, both M. aeruginosa and microalgae showed reduced growth. IC50 values for Faena® ranged from 1.022 to 2.702 mg L-1. In the microalgae + cyanobacteria bioassays, the herbicide lowered the growth rates of microalgae but stimulated the proliferation of M. aeruginosa. The joint action of both stressors affected growth rate and population dynamics, macromolecule content, and led to increased CAT and GPx levels. Faena® influenced growth rate and caused oxidative stress. On the other hand, the herbicide stimulated the synthesis of cyanotoxins, which further affected microalgal development. The experimental community was not only affected by the herbicide, but the mixed culture with cyanobacteria magnified the effects of chemical stress. These results illustrate the potential damage to phytoplankton expected in anthropically eutrophic water bodies that are also polluted by glyphosate.

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Microplastics (MPs) are critical emerging pollutants found in the environment worldwide; however, its toxicity in aquatic in amphibians, is poorly known. Thus, the aim of the present study is to assess the toxicological potential of polyethylene microplastics (PE MPs) in Physalaemus cuvieri tadpoles. According to the results, tadpoles' exposure to MP PE at concentration 60 mg/L for 7 days led to mutagenic effects, which were evidenced by the increased number of abnormalities observed in nuclear erythrocytes. The small size of erythrocytes and their nuclei area, perimeter, width, length, and radius, as well as the lower nucleus/cytoplasm ratio observed in tadpoles exposed to PE MPs confirmed its cytotoxicity. External morphological changes observed in the animal models included reduced ratio between total length and mouth-cloaca distance, caudal length, ocular area, mouth area, among others. PE MPs increased the number of melanophores in the skin and pigmentation rate in the assessed areas. Finally, PE MPs were found in gills, gastrointestinal tract, liver, muscle tissues of the tail and in the blood, a fact that confirmed MP accumulation by tadpoles. Therefore, the present study pioneering evidenced how MPs can affect the health of amphibians.

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Although tannery effluent (TE) toxicity has already been demonstrated in different vertebrate models, our knowledge about their effects on birds remains significantly incipient. Thus, the aim of the current study was to evaluate the impact of ephemeral exposure of Gallus gallus domesticus eggs to environmental predictive TE dilutions (1.4% and 6.5%). Eggs at E6 developmental stage were opened in order to assess embryos' external morphology and genotoxic biomarkers. Based on our data, embryos exposed in ovo to TE recorded higher mortality rate, lower biomass and different morphological abnormalities such as optic vesicle depigmentation, pericardial and encephalic edemas, as well as body rotation error. Embryos exposed to TE showed lower crown-rump length head and anterior-posterior length, as well as reduced beak size. Embryos exposed to the highest TE dilution (6.5%) also showed greater lower/upper limb development, larger optic vesicle area and smaller crystalline lens area than the other groups. On the other hand, differences in mitotic index were not observed between groups; however, total erythrocyte chromosomal abnormalities, mainly in metaphase and anaphase, were higher in embryos exposed to TE. These phases presented chromosome fragments formed from typical chromosome breakage, laggard chromosome and chromosome bridge. Higher Cr, Mn and Zn concentrations in embryos exposed to TE strongly suggest that the observed abnormalities were directly associated with the absorption of chemical constituents. The present study is pioneer in investigating the morphotoxic and genotoxic potential of TE (a complex mixture of various xenobiotics) in bird embryos in order to better understand the eco (toxicological) magnitude of this pollutant in aquatic ecosystems.

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