RESUMO

Abstract Worldwide, conventional agriculture makes extensive use of pesticides. Although the effects of herbicides are relatively well known in terms of environmental impacts on non-target organisms, there is very little scientific evidence regarding the impacts of herbicide residues on aquatic arthropods from tropical conservation areas. This study evaluates for the first time the toxicity of the herbicides ametryn, atrazine, and clomazone on the aquatic insect Limnocoris submontandoni (Hemiptera: Naucoridae). The lethal concentration (LC50) of herbicides was evaluated for these insects, as well as the effect of the herbicides on the insects tissues and testicles. The estimated LC50 was 1012.41, 192.42, and 46.09 mg/L for clomazone, atrazine, and ametryn, respectively. Spermatocyte and spermatid changes were observed under the effect of atrazine, and effects on spermatogenesis were observed for some concentrations of clomazone, with apparent recovery after a short time. Our results provide useful information on the effects of herbicide residues in aquatic systems. This information can help minimize the risk of long-term reproductive effects in non-target species that have been previously overlooked in ecotoxicology studies.

Resumo Em todo o mundo, a agricultura convencional faz uso extensivo de pesticidas. Embora os efeitos dos herbicidas sejam relativamente bem conhecidos em termos de impactos ambientais em organismos não-alvo, há pouca evidência científica sobre os impactos de resíduos de herbicidas em artrópodes aquáticos de áreas de conservação tropicais. Este estudo avalia pela primeira vez a toxicidade dos herbicidas ametryn, atrazine e clomazone sobre o inseto aquático Limnocoris submontandoni (Hemiptera: Naucoridae). A concentração letal (LC50) de herbicidas foi avaliada para esses insetos, bem como o efeito dos herbicidas nos tecidos e testículos dos insetos. A LC50 estimada foi de 1012,41, 192,42 e 46,09 mg/L para clomazone, atrazine e ametryn, respectivamente. Alterações nos espermatócitos e espermátides foram observadas sob o efeito de atrazine, e efeitos na espermatogênese foram observados para algumas concentrações de clomazone, com aparente recuperação após um curto período de tempo. Nossos resultados fornecem informações úteis sobre os efeitos de resíduos de herbicidas em sistemas aquáticos. Essas informações podem ajudar a minimizar o risco de efeitos reprodutivos de longo prazo em espécies não-alvo que foram negligenciadas anteriormente em estudos de ecotoxicologia.

RESUMO

Abstract Worldwide, conventional agriculture makes extensive use of pesticides. Although the effects of herbicides are relatively well known in terms of environmental impacts on non-target organisms, there is very little scientific evidence regarding the impacts of herbicide residues on aquatic arthropods from tropical conservation areas. This study evaluates for the first time the toxicity of the herbicides ametryn, atrazine, and clomazone on the aquatic insect Limnocoris submontandoni (Hemiptera: Naucoridae). The lethal concentration (LC50) of herbicides was evaluated for these insects, as well as the effect of the herbicides on the insects' tissues and testicles. The estimated LC50 was 1012.41, 192.42, and 46.09 mg/L for clomazone, atrazine, and ametryn, respectively. Spermatocyte and spermatid changes were observed under the effect of atrazine, and effects on spermatogenesis were observed for some concentrations of clomazone, with apparent recovery after a short time. Our results provide useful information on the effects of herbicide residues in aquatic systems. This information can help minimize the risk of long-term reproductive effects in non-target species that have been previously overlooked in ecotoxicology studies.

Resumo Em todo o mundo, a agricultura convencional faz uso extensivo de pesticidas. Embora os efeitos dos herbicidas sejam relativamente bem conhecidos em termos de impactos ambientais em organismos não-alvo, há pouca evidência científica sobre os impactos de resíduos de herbicidas em artrópodes aquáticos de áreas de conservação tropicais. Este estudo avalia pela primeira vez a toxicidade dos herbicidas ametryn, atrazine e clomazone sobre o inseto aquático Limnocoris submontandoni (Hemiptera: Naucoridae). A concentração letal (LC50) de herbicidas foi avaliada para esses insetos, bem como o efeito dos herbicidas nos tecidos e testículos dos insetos. A LC50 estimada foi de 1012,41, 192,42 e 46,09 mg/L para clomazone, atrazine e ametryn, respectivamente. Alterações nos espermatócitos e espermátides foram observadas sob o efeito de atrazine, e efeitos na espermatogênese foram observados para algumas concentrações de clomazone, com aparente recuperação após um curto período de tempo. Nossos resultados fornecem informações úteis sobre os efeitos de resíduos de herbicidas em sistemas aquáticos. Essas informações podem ajudar a minimizar o risco de efeitos reprodutivos de longo prazo em espécies não-alvo que foram negligenciadas anteriormente em estudos de ecotoxicologia.

Assuntos

RESUMO

The production of sugarcane bioethanol generates large volumes of vinasse, an effluent whose final disposal can produce an environmental impact that is of concern. The long-term disposal of vinasse in sugarcane fields could challenge crop management, such as the performance of traditional herbicides, by changing soil properties. This study aimed to evaluate the effect of long-term vinasse application on the field and the dissipation of atrazine and ametryn herbicides in a subtropical sugarcane agroecosystem, and to discuss the potential processes involved in it. Vinasse affected soil properties by increasing pH (12%), electrical conductivity (160%), and soil organic carbon (25%) at 0-10 cm depth of soil. Differences in the herbicide calculated sorption coefficient (Kd) varied according to the pedotransfer function applied and the herbicide type (atrazine or ametryn). During the first seven days after herbicide application, the soil underwent long-term vinasse application and increased atrazine and ametryn dissipation 45% and 33%, respectively, compared with the conventional fertilization scheme (control). The Pesticide Root Zone Model revealed that dissipation was mediated mainly by the degradation process rather than transport or other processes. The long-term application of vinasse in a typical sugarcane field of Tucumán, Argentina decreased the potential groundwater pollution of triazines and, adversely, reduced their bioavailability for weed control. For this, the present study presents original information about how long-term treatment with vinasse may require an adaptation of conventional management practices such as the application of herbicides in Argentina and other sugarcane-producing regions. Integr Environ Assess Manag 2023;00:1-12. © 2023 SETAC.

RESUMO

To obtain good control of wild poinsettia (Euphorbia heterophylla) in post-emergence in sugarcane crop, we evaluate the herbicides association on post-emergence of E. heterophylla and the ratoon cane selectivity. The experimental scheme was in randomized blocks with 6 treatments and 4 replications. The treatments were: control; ametryn + mesotrione + sulfentrazone (1,500 + 144 + 800 g i.a ha-1); ametryn + mesotrione + diclosulan (1,500 + 144 + 200 g i.a ha-1); ametryn + mesotrione (2,500 + 144 g i.a ha-1: Highest dose); ametryn + mesotrione (2,000 + 144 g i.a ha-1: Lowest dose) and ametryn + mesotrione + diuron (1,000 + 144 + 1,250 g i.a ha-1). The percentage of control, dry mass, height and percentage of germination of E. heterophylla and injury level, yield and technological quality of sugarcane were evaluated. The best control of E. heterophylla was: ametryn + mesotrione +sulfentrazone; ametryn + mesotrione + diclosulan and ametryn + mesotrione (Lowest dose). As for the ratoon cane selectivity the best yield was achieved with the association ametryn + mesotrione +diclosulan. An appropriate association of herbicide molecules provides successful control of E. heterophylla, especially the association of sulfentrazone or diclosulan together with ametryn and mesotrione.

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Thyroid hormones (TH) are essential for the metamorphosis of amphibians and their production can be influenced by environmental stressors, such as temperature fluctuations, and exposure to aquatic pollutants, such as herbicides. In the present study we evaluated the influence of different temperatures (25 and 32 °C) on the effects of the herbicide ametryn (AMT, 0 - control, 10, 50 and 200 ng.L-1) for 16 days on thyroidogenesis of bullfrog tadpoles. Higher temperature and AMT exposure caused a delay in the development of tadpoles, despite no differences were noted in weight gain and total length of the animals. Levels of triiodothyronine (T3) and thyroxine (T4) were not altered neither by AMT nor by temperature, but the highest temperature caused a decrease in total area and number of follicles in the thyroid gland. Transcript levels of thyroid hormone receptors alpha and beta (TRα and TRß) and iodothyronine deiodinase 3 (DIO3) were lower at 32 °C, which is consistent with developmental delay at the higher temperature. Tadpoles exposed to 200 ng.L-1 of AMT at 25 °C also presented delayed development, which was consistent with lower TRα and DIO3 transcript levels. Lower levels of estradiol were noted in tadpoles exposed to AMT at the higher temperature, being also possibly related to a developmental delay. This study demonstrates that higher temperature and AMT exposure impair thyroidgenesis in bullfrog tadpoles, disrupting metamorphosis.

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In addition to direct photolysis studies, in this work the second-order reaction rate constants of pesticides imidacloprid (IMD) and ametryn (AMT) with hydroxyl radicals (HO●), singlet oxygen (1O2), and triplet excited states of chromophoric dissolved organic matter (3CDOM*) were determined by kinetic competition under sunlight. IMD and AMT exhibited low photolysis quantum yields: (1.23 ± 0.07) × 10-2 and (7.99 ± 1.61) × 10-3 mol Einstein-1, respectively. In contrast, reactions with HO● radicals and 3CDOM* dominate their degradation, with 1O2 exhibiting rates three to five orders of magnitude lower. The values of kIMD,HO● and kAMT,HO● were (3.51 ± 0.06) × 109 and (4.97 ± 0.37) × 109 L mol-1 s-1, respectively, while different rate constants were obtained using anthraquinone-2-sulfonate (AQ2S) or 4-carboxybenzophenone (CBBP) as CDOM proxies. For IMD this difference was significant, with kIMD,3AQ2S* = (1.02 ± 0.08) × 109 L mol-1 s-1 and kIMD,3CBBP* = (3.17 ± 0.14) × 108 L mol-1 s-1; on the contrary, the values found for AMT are close, kAMT,3AQ2S* = (8.13 ± 0.35) × 108 L mol-1 s-1 and kAMT,3CBBP* = (7.75 ± 0.80) × 108 L mol-1 s-1. Based on these results, mathematical simulations performed with the APEX model for typical levels of water constituents (NO3-, NO2-, CO32-, TOC, pH) indicate that the half-lives of these pesticides should vary between 24.1 and 18.8 days in the waters of the Paranapanema River (São Paulo, Brazil), which can therefore be impacted by intensive agricultural activity in the region.

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There is a lack of information regarding the use of herbicides in pineapple crop, so the aim was to evaluate the growth and initial development of 'Smooth Cayenne' and 'Pérola' pineapple seedlings under application of diuron, sulfentrazone and ametryn, isolated and associated, in post-planting. The experiments were conducted in a greenhouse and the treatments consisted on application of herbicides after planting. Crop injury, leaf length,dry leaf mass and total aerial part were evaluated. The initial growth and development of the 'Smooth Cayenne' pineapple seedlings were not negatively influenced by the application of diuron. The initial growth and development of pineapple seedlings 'Pérola' was not negatively influenced by the application of diuron and ametryn, isolated or associated.

Há déficit de informações a respeito do uso de herbicidas no cultivo de abacaxi, assim objetivou-se avaliar o crescimento e desenvolvimento inicial de mudas de abacaxi ‘Smooth Cayenne’ e ‘Pérola’ sob aplicação de diuron, sulfentrazone e ametryn, isolados e em associações, em pós-plantio. Os experimentos foram conduzidos em casa de vegetação e os tratamentos foram compostos pela aplicação em pós-plantio dos herbicidas. Foram avaliados sintomas de injúria, comprimento de folha e massa seca de folha e parte aérea total. O crescimento e desenvolvimento inicial das mudas de abacaxi cultivar ‘Smooth Cayenne’ não foram influenciados negativamente pela aplicação de diuron. O crescimento e desenvolvimento inicial das mudas de abacaxi cultivar ‘Pérola’ não foi influenciado negativamente pela aplicação de diuron e ametryn, isolados ou associados entre si.

Assuntos

RESUMO

There is a lack of information regarding the use of herbicides in pineapple crop, so the aim was to evaluate the growth and initial development of 'Smooth Cayenne' and 'Pérola' pineapple seedlings under application of diuron, sulfentrazone and ametryn, isolated and associated, in post-planting. The experiments were conducted in a greenhouse and the treatments consisted on application of herbicides after planting. Crop injury, leaf length,dry leaf mass and total aerial part were evaluated. The initial growth and development of the 'Smooth Cayenne' pineapple seedlings were not negatively influenced by the application of diuron. The initial growth and development of pineapple seedlings 'Pérola' was not negatively influenced by the application of diuron and ametryn, isolated or associated.(AU)

Há déficit de informações a respeito do uso de herbicidas no cultivo de abacaxi, assim objetivou-se avaliar o crescimento e desenvolvimento inicial de mudas de abacaxi ‘Smooth Cayenne e ‘Pérola sob aplicação de diuron, sulfentrazone e ametryn, isolados e em associações, em pós-plantio. Os experimentos foram conduzidos em casa de vegetação e os tratamentos foram compostos pela aplicação em pós-plantio dos herbicidas. Foram avaliados sintomas de injúria, comprimento de folha e massa seca de folha e parte aérea total. O crescimento e desenvolvimento inicial das mudas de abacaxi cultivar ‘Smooth Cayenne não foram influenciados negativamente pela aplicação de diuron. O crescimento e desenvolvimento inicial das mudas de abacaxi cultivar ‘Pérola não foi influenciado negativamente pela aplicação de diuron e ametryn, isolados ou associados entre si.(AU)

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Herbicides are often applied to crop residues, but their fate has not been well studied. We measured herbicide washoff from sugar cane trash during simulated rainfall, at 1, 8, and 40 days after spraying (DAS), to provide insight into herbicide fate and for use in modeling. Herbicides included are commonly used in the sugar industry, either in Australia or in Brazil. Concentrations of all herbicides and applied Br tracer in washoff declined exponentially over time. The rate of washoff during rainfall declined with increasing DAS. Cumulative washoff as a function of rainfall was similar for most herbicides, although the most soluble herbicides did have more rapid washoff. Some but not all herbicides became more resistant to washoff with increasing DAS. Of the total mass washed off, 80% washed off in the first 30 mm (∼40 min) of rainfall for most herbicides. Little herbicide remained on the trash after rainfall, implying nearly complete washoff.

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A utilização de herbicidas seletivos no controle de plantas daninhas tem causado diferentes respostas em cultivares de cana-de-açúcar, e alguns produtos afetam as características fisiológicas e reduzem a atividade fotossintética. O presente trabalho teve como objetivo avaliar as variáveis fisiológicas de cultivares de cana-de-açúcar sob efeito da aplicação de herbicidas seletivos em pós-emergência. O ensaio foi desenvolvido em Jaú, SP. O delineamento experimental foi em blocos ao acaso, no esquema fatorial 5 x 4 (cultivares x herbicidas), com quatro repetições. Foram utilizadas as cultivares SP81-3250, RB855156, RB855453, RB867515, IACSP95-5000. Aos 30 dias após o plantio foram aplicados os herbicidas clomazone (1200 g.i.a. ha-1); mistura comercial de clomazone + ametryn (1000 + 1500 g.i.a. ha-1) e ametryn (3000 g.i.a. ha-1), além da testemunha sem aplicação. As cultivares IACSP95-5000 e RB867515 foram menos afetadas fisiologicamente, podendo ser consideradas seletivas aos herbicidas aplicados. Clomazone e ametryn afetaram negativamente as variáveis máxima eficiência fotoquímica do fotossistema II (Fv/Fm), índice SPAD e pigmentos fotossintéticos, porém a mistura desses herbicidas causou maiores reduções, indicando ser menos seletiva para as cultivares.

The use of selective herbicides to control weeds has caused different responses in cultivars of sugar cane, and some products affect physiological characteristics and reduce the photosynthetic activity. This study aimed to evaluate the physiological traits in cultivars of sugar cane under the effect of applying post-emergence herbicides. The test was developed in Jau, SP. The experimental design was randomized blocks in factorial scheme 5 x 4 (cultivar x herbicide) with four replications. SP81-3250, RB855156, RB855453, RB867515, IACSP95-5000 were grown in this studied. Herbicides clomazone (1200 g i.a.ha-1); commercial mixture of clomazone + ametryn (1000 + 1500 g i.a.ha-1) and ametryn (3000 g i.a.ha-1) and a control were applied at 30 days after planting. Cultivars IACSP95-5000 and RB867515 were less affected physiologically and can be considered selective to these herbicides. The application of clomazone and ametryn affected negatively the traits maximum photochemical efficiency of photosystem II (Fv/Fm), SPAD index and photosynthetic pigments, but the mixture of these herbicides caused higher reductions, indicating to be the more aggressive to the cultivars.

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In this work, an efficient degradation process for the removal of 2,4-D and ametryn, together with organic and inorganic adjuvants used in the commercial formulations of both herbicides, was developed. Although both compounds are toxic for microbial communities, ametryn is markedly more toxic than 2,4-D. In spite of this, the microbial consortium used could resist loading rates up to 31.5 mg L(-1) d(-1) of ametryn, with removal efficiencies up to 97% for both herbicides. Thus, an alternative use of this consortium could be bioaugmentation, as a tool to protect the structure and function of an activated-sludge biota against ametryn or 2,4-D shock loads. The process was carried out in a lab-scale prototype of aerobic biobarrier constructed as a compartmentalized fixed film reactor with airlift recirculation of oxygenated liquid.

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The degradation rate of the herbicide ametryn in a sandy soil was evaluated comparing the amendment of sugar cane straw cultivated with or without ametryn treatment, with non-amended soil, and sterilized soil control. Samples of 10g of soil were placed in flasks, treated with 1ml of 14C-ametryn (8mg/ml and 800Bq/mg), and were tightly closed and incubated in the dark at 23oC for 63 days. Every week the CO2 traps were changed and soil water content ajusted to 70% of soil field capacity. The mineralization rate of ametryn was 12 and 13 times higher with soil amendement of treated leaves and non-treated leaves, respectivily as compared to non amended soil. The leaf amendement led to a greater number of microrganism colonies, higher microbial activity and more ametryn metabolites than non-amended or sterilized soil. The results suggest that ametryn degrades mainy through microbial co-metabolism.

A degradabilidade do herbicida ametrina foi determinada em solo Areia Quartzosa com e sem adição de folhas secas de cana-de-açúcar, cultivada em campo, tratada ou não tratada com herbicida ametrina e em solo esterilizado. Amostras de 10g de solo com umidade ajustada à 70% da capacidade de campo e tratadas com 1ml de 14C-ametrina, na concentração de 8mg/ml e atividade específica de 800Bq/mg, foram distribuídas em frascos com tampa e incubadas em ambiente escuro, a 23oC, por 63 dias. Comparando a mineralização da ametrina em solo sem adição e com adição de folhas de cana-de-açúcar tratada ou não tratadas, observou-se 12 a 13 vezes mais 14CO2 desprendido em solos com a adição de palha. Nestes tratamentos foi observado, após 63 dias de incubação, maior número de microrgarnismos, maior atividade microbiana e formação de maior número de metabólitos, quando comparados com os tratamentos sem adição de palha ou o esterilizado. Os resultados sugerem que a degradação da ametrina é de origem microbiológica e ocorre por cometabolismo.

RESUMO

The degradation rate of the herbicide ametryn in a sandy soil was evaluated comparing the amendment of sugar cane straw cultivated with or without ametryn treatment, with non-amended soil, and sterilized soil control. Samples of 10g of soil were placed in flasks, treated with 1ml of 14C-ametryn (8mg/ml and 800Bq/mg), and were tightly closed and incubated in the dark at 23oC for 63 days. Every week the CO2 traps were changed and soil water content ajusted to 70% of soil field capacity. The mineralization rate of ametryn was 12 and 13 times higher with soil amendement of treated leaves and non-treated leaves, respectivily as compared to non amended soil. The leaf amendement led to a greater number of microrganism colonies, higher microbial activity and more ametryn metabolites than non-amended or sterilized soil. The results suggest that ametryn degrades mainy through microbial co-metabolism.

A degradabilidade do herbicida ametrina foi determinada em solo Areia Quartzosa com e sem adição de folhas secas de cana-de-açúcar, cultivada em campo, tratada ou não tratada com herbicida ametrina e em solo esterilizado. Amostras de 10g de solo com umidade ajustada à 70% da capacidade de campo e tratadas com 1ml de 14C-ametrina, na concentração de 8mg/ml e atividade específica de 800Bq/mg, foram distribuídas em frascos com tampa e incubadas em ambiente escuro, a 23oC, por 63 dias. Comparando a mineralização da ametrina em solo sem adição e com adição de folhas de cana-de-açúcar tratada ou não tratadas, observou-se 12 a 13 vezes mais 14CO2 desprendido em solos com a adição de palha. Nestes tratamentos foi observado, após 63 dias de incubação, maior número de microrgarnismos, maior atividade microbiana e formação de maior número de metabólitos, quando comparados com os tratamentos sem adição de palha ou o esterilizado. Os resultados sugerem que a degradação da ametrina é de origem microbiológica e ocorre por cometabolismo.