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Objective: Many studies have observed different characteristics among productive systems in the rural territories of Latin America. Therefore, understanding and characterizing them while they function plays an essential role in determining their relationship between development and environment. A study has been conducted in the Orellana province of NE Ecuador to determine their typology and then classify them according to the variables that describe their main traits or attributes using cluster analysis (CA). Materials and Methods: A survey was structured to investigate physical, productive, environmental, as well as socioeconomic character variables, which were subsequently applied to a random sample of the 5,963 agricultural productive units (APUs) through face-to-face contact with producers during an in situ visit to their farms. Result: The CA allowed us to identify three typologies of APUs in the Orellana Province. The first has been Type 1, which is denominated as the most conventional (40%), while Type 2 uses more efficient natural resources but represents an amount of only 9.4%. In contrast, type 3 (50.6%) depends on a significant part of local or national development programs. Conclusion: All groups indicated some peculiarities in common, as there were marked differences in the use and distribution of land as well as production methods among them. Consequently, this pioneering study allowed us to identify different production methods. Therefore, we encourage local and national governments to establish policies for natural resource conservation in such high-diversity zones.
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Resumen: Desde la perspectiva de la ecología política este artículo da cuenta de las barreras existentes para la gobernanza ambiental en la Sierra de La Macarena. Este tipo de gobernanza, que hace referencia a una gestión participativa y democrática en la gestión del ambiente, está implícita en la reforma rural integral acordada en el proceso de paz de 2016. Sin embargo, a pesar del reconocimiento de la Selva Ama zónica como Sujeto de Derechos y de distintos espacios de concertación planteados por organizaciones sociales, la violencia que emergió tras la firma de los acuerdos ha supuesto que la superposición de conflictos socioambientales que configuran la región, se profundicen. La tala y quema indiscriminada de enormes porciones de bosque amazónico a manos de empresas ganaderas y la respuesta militarizada que afecta principalmente a comunidades campesinas ha sido la principal causa para que en la actualidad haya una gestión ambiental sin gobernanza. El artículo pro pone elementos analíticos que permitan no solo entender, sino también establecer estrategias que permitan el reacomodo de la gestión ecológica de la región.
Abstract: From a political ecology perspective, this article reports on the existing barriers to environmental governance in the Sierra de La Macarena. This type of gover nance, which refers to participatory and democratic management in environ mental management, is implicit in the comprehensive rural reform agreed to in the 2016 peace process. However, the emerging violence following the signing of the agreements has meant that overlapping socio-environmental conflicts are deepening. The indiscriminate logging and burning of huge portions of the Am azonian forest by cattle ranching companies and the militarized response, by affecting mainly peasant communities, conflict has been the main expression. The article proposes analytical elements that allow not only to understand, but also to establish strategies that permit readjustment of the ecological manage ment of the region.
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The objective of this study was to evaluate ecological aspects of Mansonia species before the construction of hydroelectric plants on the Madeira River, and thus enable the assessment of the impact of these projects on mosquitoes. A total of 199 samplings were carried out between November 2003 and August 2004, using the technique of attraction with protection. Temporal distribution was evaluated from monthly incidence values obtained from the bite index per man/hour. Relative abundance was subsequently calculated to evaluate the spatial distribution of species, according to land use and municipal districts; furthermore, the pattern of hematophagous activity was evaluated from 12-h and 4-h samplings. The data were analyzed according to the negative binomial distribution and generalized linear models to estimate the influence of environmental factors on the presence and abundance of Mansonia. A total of 1479 specimens were collected, distributed among four species-Mansonia titillans (87%), Mansonia humeralis (6.3%), Mansonia amazonensis (6%), and Mansonia indubitans (0.5%), and spatial distribution analysis showed Ma. titillans to be dominant. Hematophagous activity had peaks between 6:00 p.m. and 8:00 p.m. and species incidence was higher during the rainy season and in areas where domestic animals are raised. Therefore, the region studied presented characteristics favorable to the reproduction of Mansonia even before the construction of the hydroelectric plants and after construction, these conditions were enhanced, due to the increase in the availability of breeding sites for immatures and blood sources for females, as a consequence of changes in the environment.
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The first two anophthalmic species of spiders of the genus Ochyrocera Simon, 1892, are described for caves located in the iron formation of Floresta Nacional (FLONA) de Carajás in southeastern Pará State, Brazil. The caves are located in the municipalities of Parauapebas and Canaã dos Carajás, in the eastern portion of the Amazon Forest domain. Ochyrocera ritxoco sp. nov. and O. ritxoo sp. nov. are described based on males and females. The species have similar body characteristics with the total absence of eyes and complete depigmentation, characteristics that indicate possible evolution in subterranean environments , and thus are classified as troglobites. Each species is associated with a single geomorphological unit (mountain range), with Ochyrocera ritxoco sp. nov. being restricted to caves of Serra Norte (North Mountain) and O. ritxoo sp. nov. to caves of Serra Sul (South Mountain). Both species were collected in aphotic zones of the caves. Small and tangled webs of O. ritxoco sp. nov. were observed under blocks of stone in the soil or in cracks of the walls.
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Dichotomius (Dichotomius) quadrilobatus new species (Coleoptera: Scarabaeidae: Scarabaeinae: Dichotomiini), from western Amazonia (Brazil, Colombia, Ecuador and Peru) is described and its putative systematic position within the Dichotomius boreus species group is discussed. An updated identification key to the species of the Dichotomius boreus species group is provided. Additionally, Dichotomius (Selenocopris) fortepunctatus Luederwaldt, 1923 is recorded for the first time in Colombia.
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Besouros , Animais , ColômbiaRESUMO
The first two anophthalmic species of spiders of the genus Ochyrocera Simon, 1892, are described for caves located in the iron formation of Floresta Nacional (FLONA) de Carajás in southeastern Pará State, Brazil. The caves are located in the municipalities of Parauapebas and Canaã dos Carajás, in the eastern portion of the Amazon Forest domain. Ochyrocera ritxoco sp. nov. and O. ritxoo sp. nov. are described based on males and females. The species have similar body characteristics with the total absence of eyes and complete depigmentation, characteristics that indicate possible evolution in subterranean environments , and thus are classified as troglobites. Each species is associated with a single geomorphological unit (mountain range), with Ochyrocera ritxoco sp. nov. being restricted to caves of Serra Norte (North Mountain) and O. ritxoo sp. nov. to caves of Serra Sul (South Mountain). Both species were collected in aphotic zones of the caves. Small and tangled webs of O. ritxoco sp. nov. were observed under blocks of stone in the soil or in cracks of the walls.
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The epidemic transmission of Zika virus (ZIKV) in Brazil has been identified as a cause of microcephaly and other neurological malformations in the babies of ZIKV-infected women. The frequency of adverse outcomes of Zika virus infection (ZIKVi) in pregnancy differs depending on the characteristics of exposure to infection, the time of recruitment of research participants, and the outcomes to be observed. This study provides a descriptive analysis-from the onset of symptoms to delivery-of a cohort registered as having maternal ZIKVi in pregnancy, from November 2015 to December 2016. Suspected cases were registered at a referral center for infectious and tropical diseases in Manaus, in the Amazonian region of Brazil. Of 834 women notified, 762 women with confirmed pregnancies were enrolled. Reverse-transcriptase polymerase chain reaction (RT-PCR) confirmed ZIKVi in 42.3% of the cohort. In 35.2% of the cohort, ZIKV was the sole infection identified. Severe adverse pregnancy outcomes (miscarriage, stillbirth, or microcephaly) were observed in both RT-PCR ZIKV-positive (5.0%) and ZIKV-negative (1.8%) cases (RR 3.1; 95% IC 1.4-7.3; p < 0.05), especially during the first trimester of pregnancy (RR 6.2, 95% IC 2.3-16.5; p < 0.001). Although other infectious rash diseases were observed in the pregnant women in the study, having confirmed maternal ZIKVi was the most important risk factor for serious adverse pregnancy events.
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Exantema/epidemiologia , Exantema/etiologia , Complicações Infecciosas na Gravidez/epidemiologia , Complicações Infecciosas na Gravidez/virologia , Infecção por Zika virus/complicações , Infecção por Zika virus/epidemiologia , Zika virus/fisiologia , Adolescente , Adulto , Brasil/epidemiologia , Estudos de Coortes , Feminino , Humanos , Incidência , Avaliação de Resultados da Assistência ao Paciente , Gravidez , Análise Espaço-Temporal , Adulto Jovem , Infecção por Zika virus/virologiaRESUMO
Bothrops brazili is a pitviper from Amazonian region, responsible for many accidents in Peru. Despite its relevance, its venom has not been extensively characterized. In the present work, Bothrops brazili venom (BbV) components were analyzed by RP-HPLC, SDS-PAGE and MALDI-TOF/TOF. Approximately 37 proteins were identified, belonging to 7 families. Snake venom metalloproteinases (SVMPs) were the most abundant proteins of the venom (33.05%), followed by snake venom serine proteinases (SVSPs, 26.11%), phospholipases A2 (PLA2, 25.57%), snake C-type lectins (CTLs, 9.61%), L-aminoacid oxidase (LAAO, 3.80%), cystein-rich secretory proteins (CRISP, 1.67%) and Bradykinin-potentiating peptide (BPP, 0.20%). In vitro enzymatic activities of BbV showed high levels of SVMP activity and reduced Hyal activity in comparison with other bothropic venoms. Furthermore, BbV reduced VERO cells viability. ELISA and Western Blotting showed that both Peruvian and Brazilian bothropic antivenoms were able to recognize BbV components. This work provides an overview of BbV venom content and indicates a potential efficiency of Peruvian and Brazilian antivenoms to treat accidents with this species.
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Bothrops , Venenos de Crotalídeos/toxicidade , Animais , Antivenenos , Western Blotting , Brasil , Chlorocebus aethiops , Cromatografia Líquida de Alta Pressão , Venenos de Crotalídeos/metabolismo , Eletroforese em Gel de Poliacrilamida , L-Aminoácido Oxidase/metabolismo , Peru , Fosfolipases A2/química , Proteômica , Serina Proteases/metabolismo , Células VeroRESUMO
The last instar larva and the pupa of Hybolabus amazonicus Voss, 1925 are described and illustrated, based upon adults and immatures collected in the Amazonian Region (Acre and Amazonas, Brazil). The larvae live and develop inside a leaf-roll made by the female weevil. Although the species has already been reported damaging leaves of the Brazil nut (Bertholletia excelsa Humb. & Bonpl., Lecythidaceae) in the states of Acre and Amazonas, the preimaginal stages have not been formerly described. These are the first descriptions of larva and pupa of a species of the genus Hybolabus Jekel, 1860.
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Animais , Bertholletia , Gorgulhos/anatomia & histologia , Gorgulhos/crescimento & desenvolvimento , Pupa/anatomia & histologia , Brasil , Ecossistema AmazônicoRESUMO
The last instar larva and the pupa of Hybolabus amazonicus Voss, 1925 are described and illustrated, based upon adults and immatures collected in the Amazonian Region (Acre and Amazonas, Brazil). The larvae live and develop inside a leaf-roll made by the female weevil. Although the species has already been reported damaging leaves of the Brazil nut (Bertholletia excelsa Humb. & Bonpl., Lecythidaceae) in the states of Acre and Amazonas, the preimaginal stages have not been formerly described. These are the first descriptions of larva and pupa of a species of the genus Hybolabus Jekel, 1860.(AU)
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Animais , Gorgulhos/anatomia & histologia , Gorgulhos/crescimento & desenvolvimento , Pupa/anatomia & histologia , Bertholletia , Ecossistema Amazônico , BrasilRESUMO
Abstract The last instar larva and the pupa of Hybolabus amazonicusVoss, 1925 are described and illustrated, based upon adults and immatures collected in the Amazonian Region (Acre and Amazonas, Brazil). The larvae live and develop inside a leaf-roll made by the female weevil. Although the species has already been reported damaging leaves of the Brazil nut (Bertholletia excelsa Humb. & Bonpl., Lecythidaceae) in the states of Acre and Amazonas, the preimaginal stages have not been formerly described. These are the first descriptions of larva and pupa of a species of the genus Hybolabus Jekel, 1860.
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Abstract INTRODUCTION: The Amazon tropical rainforest has the most dense and diverse ecosystem worldwide. A few studies have addressed rodent-borne diseases as potential hazards to humans in this region. METHODS: A retrospective survey was conducted using enzyme-linked immunosorbent assay for detecting mammarenavirus and orthohantavirus antibodies in 206 samples collected from rural settlers of the Brazilian Western Amazonian region. RESULTS: Six (2.91%) individuals in the age group of 16 to 36 years were found to possess antibodies against mammarenavirus. CONCLUSION: Evidence of previous exposure to mammarenavirus in the rural population points to its silent circulation in this region.
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Humanos , Animais , Masculino , Feminino , Lactente , Pré-Escolar , Criança , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Adulto Jovem , Arenaviridae/imunologia , Roedores/virologia , Reservatórios de Doenças/veterinária , Orthohepadnavirus/imunologia , Infecções por Arenaviridae/epidemiologia , Hepatite Viral Humana/epidemiologia , Anticorpos Antivirais/sangue , Arenaviridae/classificação , Roedores/classificação , População Rural , Fatores Socioeconômicos , Brasil/epidemiologia , Estudos Retrospectivos , Orthohepadnavirus/classificação , Infecções por Arenaviridae/diagnóstico , Infecções por Arenaviridae/transmissão , Hepatite Viral Humana/diagnóstico , Hepatite Viral Humana/transmissão , Pessoa de Meia-IdadeRESUMO
ABSTRACT The oil palm is an economically important crop cultivated in the North of Brazil. Damage caused by insects is one of the main causes of reduced productivity for the oil palm. Before this research, only the beetles of the family Curculionidae were considered to be oil palm pests in Brazil. However, for the first time, we report on the damage caused by a giant rhinoceros beetle to oil palm plantations in Pará, Brazil. The beetle was identified as Golofa claviger (Linnaeus, 1771), which has a single record in Brazil (Pará) but is widely distributed in South America. The species occurs in an unprecedentedly high abundance of local specimens. The attacks are concentrated on the central cluster of young palms. Feeding behavior is identified as the main cause of the damage as the beetles use their mouthparts to rip the plant tissues, causing wedge-shaped cuts on young fronds that have not yet unfurled. After an attack, the leaflets of the unfurled fronds are partially destroyed.
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Abstract INTRODUCTION: Elimination of malaria in areas of interrupted transmission warrants careful case assessment to avoid the reintroduction of this disease. Occasional malaria cases are reported among visitors of the Atlantic Forest area of Brazil, while data on residents of this area are scarce. METHODS: A sectional study was carried out to examine 324 individuals living in a municipality where autochthonous cases were detected. RESULTS: Asymptomatic Plasmodium infections were detected in 2.8% of the individuals by polymerase chain reaction (PCR), with one case of P. falciparum (0.3%), two cases of P. vivax (0.6%), and six cases of P. malariae (1.9%). The thick blood smears were negative in all individuals. Serological tests performed in 314 subjects were reactive in 11.1%, with 3.5% for P. falciparum and 7.7% for P. vivax. A subsample of 42 reactive individuals for any Plasmodium species showed P. malariae in 30.9% of specimens. Individuals who entered the Atlantic Forest region were 2.7 times more likely to exhibit reactive serology for P. vivax compared with individuals who did not enter this region (p<0.05). Children <15 years had a higher chance of reactive serology for P. falciparum and P. vivax than individuals ≥15 years of age (p<0.05). Individuals living in the Paraiso district had a higher chance of reactive serology for P. vivax compared to other districts (p<0.05). No associations were found between sex, past exposure to malaria, or serological response to antibodies of any Plasmodium species. CONCLUSIONS: The implications of these results for the elimination of malaria were discussed.
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Humanos , Masculino , Feminino , Adulto , Idoso , Idoso de 80 Anos ou mais , Malária Vivax/diagnóstico , Malária Vivax/transmissão , Malária Falciparum/diagnóstico , Malária Falciparum/transmissão , Brasil/epidemiologia , Ensaio de Imunoadsorção Enzimática , Reação em Cadeia da Polimerase , Estudos Transversais , DNA de Protozoário/análise , Malária Vivax/epidemiologia , Malária Falciparum/epidemiologia , Infecções Assintomáticas/epidemiologia , Antígenos de Protozoários/imunologiaRESUMO
The microbiota from artisanal cheeses produced in the Amazonian region is evaluated. Samples of artisanal cheeses were obtained from markets in Conceição do Araguaia and Redenção (Pará, Brazil) over rainy and dry seasons, and their biodiversity was assessed by culture-dependent and culture-independent methods. Mean counts of lactic acid bacteria (LAB) in cheeses ranged from 7.32 to 8.84 log CFU/g, for both seasons. Members of genera Lactobacillus, Lactococcus, Weissella, Enterococcus, Pediococcus, and Leuconostoc were predominant. The amplification of the 16S rRNA V6-V9 region, followed by a temporal temperature-gradient gel electrophoresis (TTGE) and sequencing of the TTGE bands revealed important differences in the microbial composition variability between samples from the two seasons and among cheese samples analyzed. TTGE showed the presence of microorganisms that are frequently found in cheese, such as L. lactis subsp. lactis, as well as other non-usual species, such as Macrococcus caseolyticus and Corynebacterium variabile. Moreover, TTGE analysis revealed the presence of microorganisms that have been isolated from other types of foods (Paralactobacillus selangorenses) along with some not usually found in foods, such as Exiguobacterium acetylicum, plus the presence of pathogenic microorganisms (Granulicatella elegans and Aerococcus sanguinicola). The present molecular approaches combined with culture-dependent methods provided a more detailed description of the microbial ecology of traditional cheeses from the Amazonian region in northern Brazil.
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Bactérias/classificação , Queijo/microbiologia , Chuva , Estações do Ano , Bactérias/genética , Bactérias/isolamento & purificação , Biodiversidade , Brasil , Contagem de Colônia Microbiana , Microbiologia de Alimentos/métodos , Floresta Úmida , RibotipagemRESUMO
BACKGROUND: Malaria continues being a public health problem worldwide. Plasmodium vivax is the species causing the largest number of cases of malaria in Asia and South America. Due to the lack of a completely effective anti-malarial vaccine, controlling this disease has been based on transmission vector management, rapid diagnosis and suitable treatment. However, parasite resistance to anti-malarial drugs has become a major yet-to-be-overcome challenge. This study was thus aimed at determining pvmdr1, pvdhfr, pvdhps and pvcrt-o gene mutations and haplotypes from field samples obtained from an endemic area in the Colombian Amazonian region. METHODS: Fifty samples of parasite DNA infected by a single P. vivax strain from symptomatic patients from the Amazonas department in Colombia were analysed by PCR and the pvdhfr, pvdhps, pvmdr1 and pvcrt-o genes were sequenced. Diversity estimators were calculated from the sequences and the haplotypes circulating in the Colombian Amazonian region were obtained. CONCLUSION: pvdhfr, pvdhps, pvmdr1 and pvcrt-o genes in the Colombian Amazonian region are characterized by low genetic diversity. Some resistance-associated mutations were found circulating in this population. New variants are also being reported. A selective sweep signal was located in pvdhfr and pvmdr1 genes, suggesting that these mutations (or some of them) could be providing an adaptive advantage.
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Antimaláricos/farmacologia , Resistência a Medicamentos/genética , Mutação , Plasmodium vivax/genética , Polimorfismo Genético , Proteínas de Protozoários/genética , Colômbia , Haplótipos , Reação em Cadeia da PolimeraseRESUMO
Seven new species of the spider genus Ochyrocera from cave areas in Floresta Nacional de Carajás (state of Pará, northern Brazil) are described: Ochyrocera varyssp. n., Ochyrocera atlachnachasp. n., Ochyrocera laracnasp. n., Ochyrocera aragoguesp. n., Ochyrocera misspidersp. n., Ochyrocera charlottesp. n., and Ochyrocera ungoliantsp. n. Two groups of the species are discussed, the quinquivittata group that include specimens with an apparently bifid retrolateral apophysis in the cymbium of the male palp and the arietina group, here proposed, that include those specimens with an entire cymbium, with no retrolateral apophysis, in the male palp. Although these species were abundant inside caves, the examined specimens do not have troglomorphic characteristics and can be classified as edaphic troglophile species, capable of completing its life cycle in soil, shallow subterranean habitats, or caves.
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Seven new species of the spider genus Ochyrocera from cave areas in Floresta Nacional de Carajas (state of Para, northern Brazil) are described: Ochyrocera varys sp. n., Ochyrocera atlachnacha sp. n., Ochyrocera laracna sp. n., Ochyrocera aragogue sp. n., Ochyrocera misspider sp. n., Ochyrocera charlotte sp. n., and Ochyrocera ungoliant sp. n. Two groups of the species are discussed, the quinquivittata group that include specimens with an apparently bifid retrolateral apophysis in the cymbium of the male palp and the arietina group, here proposed, that include those specimens with an entire cymbium, with no retrolateral apophysis, in the male palp. Although these species were abundant inside caves, the examined specimens do not have troglomorphic characteristics and can be classified as edaphic troglophile species, capable of completing its life cycle in soil, shallow subterranean habitats, or caves.
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A new Diaptomidae species is presented from the Neotropical region. It was found in two Amazonian lakes, Ressaca and Arapujá, both in Pará State, Brazil. The lakes are 400 km apart and threatened by the building of reservoirs for hydropower generation and pollution by human settlements. The new species resembles N. paraensis Dussart & Robertson, 1984, but it can be distinguished from this species and other congeners in having a special process on the fifth leg basis of the male, by the place of insertion of lateral spine in the last segment of right P5 of male, the shape and relationship between length and width of segments of male and female P5 exopodite 2 with stout inner process bearing short setules and outer small spine, exopodite 3, with two terminal setae, outer smaller; endopodite 1-segmented with one subterminal seta and oblique comb of spinules, the presence of a line of dorsal spinules at the distal margin of thoracic somites in both sexes. A brief comparison with other Notodiaptomus species is presented in the discussion.
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Abstract Objectives: to analyze the dimensions of policy, structure and organization in the construction of Redes de Atenção à Saúde (RAS) (healthcare networks) in the health region of Manaus, Entorno and Alto Rio Negro, focusing on Atenção Primária à Saúde (APS) (primary healthcare) and Rede de Urgência e Emergência (RUE) (urgency and emergency network). Methods: a case study with multidimensional analysis using quantitative and qualitative approach carried out in the first semester of 2016. Results: 37 interviewed key informants, such as managers (States, Cities and Regionals), providers and civil society. The reality was marked by the difficulties in the implementation of RAS's with centralized decision-making powers of State level. Perception of insufficient human resources of limited installed capacity, particularly in the APS, with insufficient priority given to this level of care. Concentration of services in Manaus, priority for urgency and emergency actions expressed in the greatest investment in human resources and mate-rials allocated at this level of care, lack of proposals for promoting equity. Conclusions: the Region was unable to implement RAS to respond to the health demands in the region. The necessity to overcome the dependency relation with the cities and the State and to strengthen its protagonism and fulfillits roles in management network, instituting a plan capable of strengthening APS to be committed in reducing iniquities and with adequate responses in health needs.
Resumo Objetivos: analisar as dimensões da política, estrutura e organização na construção das redes de atenção à saúde (RAS) na região de saúde de Manaus, Entorno e Alto Rio Negro, enfocando a atenção primária à saúde (APS) e rede de urgência e emergência (RUE). Métodos: estudo de caso com análise multidimensional, utilizando abordagem quantitativa e qualitativa, realizado no primeiro semestre de 2016. Resultados: entrevistados 37 informantes-chaves, sendo gestores (estaduais, municipais e regionais), prestadores e sociedade civil. Realidade marcada por dificuldades na implantação das RAS, com poderes decisórios centralizados no nível estadual. Percepção de insuficiência de recursos humanos de limitação na capacidade instalada, em particular na APS, com insuficiente prioridade dada a este nível de cuidado. Concentração de serviços em Manaus, prioridade para ações de urgência e emergência, expressas em maior investimento de recursos humanos e materiais alocados nesse nível de atenção, ausência de propostas para promoção da equidade. Conclusões: região não conseguiu implantar funcionamento das RAS para responder às demandas em saúde na região. Necessidade de superar relação de dependência entre municípios e estado e de potencializar seu protagonismo e o cumprimento dos seus papéis na gestão das redes, instituindo um planejamento capaz de fortalecer a APS comprometida com a redução das iniquidades e com respostas adequadas às necessidades de saúde.