RESUMO
Aging is one of the risk factors involved in the development of erectile dysfunction (ED). Growing evidence suggests that oxidative stress is the critical mediator of changes in endothelial function and penile vascular tone in the aging process. Thus, reducing reactive oxygen species (ROS) levels may preserve the bioactivity of the penile vasculature. Antioxidant compounds, such as carvacrol, limit the damage caused by ROS and, therefore, benefit the treatment of ED. Thus, this study aims to evaluate the effects of carvacrol on ED using the D-( +)-galactose aging model. The animals were divided into five groups: control, D-( +)-galactose 150 mg/kg, carvacrol 50 mg/kg or 100 mg/kg, and sildenafil 1.5 mg/kg treated daily for 8 weeks. The physiological, functional, and morphological characteristics of aging-associated ED were evaluated after treatment with carvacrol. Carvacrol prevented ED in a D-( +)-galactose-induced aging model by reducing hypercontractility, enhancing endothelial dysfunction in the rat corpus cavernosum, and improving endothelial health of rat cavernous endothelial cells. In addition, carvacrol prevented the destruction of erectile components essential for penile erection and promoted a reduction of penile tissue senescence, probably through mechanisms that involve the harmful modulation of oxidative stress. Carvacrol significantly improved the erectile function of rats in a D-( +)-galactose-induced aging model and has excellent potential as a new therapeutic alternative in treating erectile dysfunction.
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In humans, aging is characterized by a gradual decline of physical and psychological functions, with the concomitant onset of chronic-degenerative diseases, which ultimately lead to death. The study of Hutchinson-Gilford progeria syndrome (HGPS), a premature aging disorder that recapitulates several features of natural aging, has provided important insights into deciphering the aging process. The genetic origin of HGPS is a de novo point mutation in the LMNA gene that drives the synthesis of progerin, mutant version of lamin A. Progerin is aberrantly anchored to the nuclear envelope disrupting a plethora of molecular processes; nonetheless, how progerin exerts a cascade of deleterious alterations at the cellular and systemic levels is not fully understood. Over the past decade, the use of different cellular and animal models for HGPS has allowed the identification of the molecular mechanisms underlying HGPS, paving the way towards the development of therapeutic treatments against the disease. In this review, we present an updated overview of the biology of HGPS, including its clinical features, description of key cellular processes affected by progerin (nuclear morphology and function, nucleolar activity, mitochondrial function, protein nucleocytoplasmic trafficking and telomere homeostasis), as well as discussion of the therapeutic strategies under development.
Assuntos
Progéria , Animais , Humanos , Progéria/terapia , Progéria/tratamento farmacológico , Envelhecimento , Mitocôndrias/metabolismoRESUMO
ABSTRACT The accelerated aging process is incorporated into the design and development of intravascular catheters to assess their reliability assuring that this medical device is safe and effective for the intended use during their shelf life. The accelerated aging process is based on a common approach that assumes that the rate of aging increases by a factor of 2^(∆T/10), where ∆T is the temperature increment. However, with the life data obtained from this empirical method is difficult to do inferences about reliability. This paper presents an accelerated destructive degradation test using thermal stress to obtain degradation data directly relates reliability to critical performance characteristic, which is the tensile strength in the intravascular catheter tip considered as a critical concern in patients' safety. The degradation data model is given by a stochastic Wiener process with the drift parameter being represented as Arrhenius function. The parameters of the Wiener process and Arrhenius function are estimated using maximum likelihood; these parameters are used to estimate the first-passage time (time to failure) distribution when the intravascular catheters degradation path reaches a tensile strength critical value in each thermal stress level. Based on this, a complete product reliability assessment is performed and presented.
RESUMEN El proceso de envejecimiento acelerado es incorporado en el diseño y desarrollo de catéteres intravasculares para evaluar su confiabilidad y asegurar que el dispositivo medico es seguro y efectivo para su uso durante su vida de estante. Este proceso está basado en un enfoque que asume que la tasa de envejecimiento se incrementa por un factor de 2^(∆T/10), en donde ∆T es el incremento de temperatura. Sin embargo, con los datos de vida obtenidos de este método empírico resulta complicado realizar inferencias sobre la confiabilidad del dispositivo. Este articulo presenta una prueba de degradación acelerada destructiva que considera un estrés termal para obtener datos de degradación que se relaciona directamente la confiabilidad con la resistencia tensil de la punta de un catéter intravascular y que es considerada como una característica critica para la seguridad de los pacientes. El modelo de degradación esta dado por un proceso estocástico Wiener, con el parámetro de deriva representado la relación de Arrhenius. Los parámetros del proceso Wiener y la relación de Arrhenius son estimados mediante máxima verosimilitud; estos parámetros son usados para estimar la distribución de primer paso, la cual se caracteriza cuando la resistencia de un catéter alcanza el nivel crítico de resistencia en cada nivel de estrés. Considerando esto, se lleva a cabo y se presenta una evaluación de confiabilidad completa del producto.
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Dynamic mechanical analysis (DMA) is one of the most common methods employed to study a material's viscoelastic properties. The effect of thermal aging on plain epoxy and a fique fabric-reinforced epoxy composite was investigated by comparing the mass loss, morphologies, and DMA properties of aged and unaged samples. In fact, thermal aging presents a big challenge for the high-temperature applications of natural fiber composites. In this work, both plain epoxy and fique fabric-reinforced epoxy composite were found to have different molecular mobility. This leads to distinct transition regions, with different changes in intensity caused by external loadings from time-aging. Three exponentially modified Gauss distribution functions (EMGs) were applied to loss factor curves of fique fabric-reinforced epoxy composite and plain epoxy, which allowed identifying three possible mobility ranges. From these results it was proposed that the thermal degradation behavior of natural fibers, especially fique fiber and their composites, might be assessed, based on their structural characteristics and mechanical properties.
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Schizophrenia (SZ) is a chronic debilitating disease. Subjects with SZ have significant shorter life expectancy. Growing evidence suggests that a process of pathological accelerated aging occurs in SZ, leading to early development of severe clinical diseases and worse morbimortality. Furthermore, unaffected relatives can share certain endophenotypes with subjects with SZ. We aim to characterize accelerated aging as a possible endophenotype of schizophrenia by using a machine learning (ML) model of peripheral biomarkers to accurately differentiate subjects with SZ (n = 35), their unaffected siblings (SB, n = 36) and healthy controls (HC, n = 47). We used a random forest algorithm that included biomarkers related to aging: eotaxins CCL-11 and CCL-24; the oxidative stress markers thiobarbituric acid-reactive substances (TBARS), protein carbonyl content (PCC), glutathione peroxidase (GPx); and telomere length (TL). The ML algorithm of biomarkers was able to distinguish individuals with SZ from HC with prediction accuracy of 79.7%, SZ from SB with 62.5% accuracy and SB from HC with 75.5% accuracy. These results support the hypothesis that a pathological accelerated aging might occur in SZ, and this pathological aging could be an endophenotype of the disease, once this profile was also observed in SB, suggesting that SB might suffer from an accelerated aging in some level.
Assuntos
Esquizofrenia , Envelhecimento , Endofenótipos , Humanos , Carbonilação Proteica , Esquizofrenia/genética , IrmãosRESUMO
Objective: The objective of the present in-vitro study was to verify the efficacy of two spectrophotometer-based shade matching systems for color matching of ceramics after artificial accelerated aging (AAA). Material and Methods: The ceramics used were porcelain laminated veneers. Seventy standard-shaped discs (thickness x diameter: 0.5 mm x 10 mm) of B1 shades were used. Based upon the type of resin cement used, the specimens were divided into seven groups (10/group). The following light-cured cements were used: RelyX-Veneer (L-RV), Variolink-Veneer (L-VV), and Variolink-Esthetic (L-VE). The dual-cured cements were: RelyX Ultimate (D-RU), RelyX-Unicem (D-RC), and Variolink-Esthetics DC (D-VE). The control group consisted of ceramic only. All specimens were thermocycled in water for 3,500 cycles between 5 oC -55 oC, with dwell times of 30 s in each bath and a transfer time of 10 s between baths. All specimens were thermocycled in water for 3,500 cycles between 5 oC and 55 oC, and color measurement was done using the VITA Easyshade and ColorEye spectrophotometers. Baseline color reading was performed 24-hours after cementation. Differences in color (DE) of EasyShade and ColorEye before and after AAA were determined and compared statistically. Group comparisons were done using the paired t-tests. Level of significance was set at P< 0.05. Results: The mean differences in color (ΔE) values obtained from EasyShade spectrophotometer for light- and dual-cured cements, were 0.843±0.89 (L-RV ), 4.11±0.69 (L-VV) and 0.833±0.47 (L-VE); and 2.22±0.64 (D-RU), 3.37±0.83 (D-RC) and 0.38±0.92 (D-VE), respectively. The mean differences in color (ΔE) values obtained from ColorEye spectrophotometer for light- and dual-cured cements, were 0.68±0.86 (L-RV ), 4.55±0.83 (L-VV) and 2.68±0.26 (L-VE); and 2.06±0.84 (D-RU), 1.8±1.08 (D-RC) and 0.96±0.71 (D-VE), respectively. There was no significant difference in the mean DE values among the groups. Conclusion: The VITA Easyshade and ColorEye shade matching systems are comparable in terms of their efficacy for color matching of cerammics after AAA.(AU)
Objetivos: O objetivo do presente estudo in vitro foi verificar a eficácia de dois espectrofotômetros baseados em escalas de cor para avaliação de cor de cerâmicas após envelhecimento artificial acelerado (EAA). Material e Métodos: As cerâmicas utilizadas foram laminados cerâmicos. Foram usados setenta discos de formato padrão (0,5mm de espessura x 10 mm de diâmetro) de tonalidades B1. Com base no tipo de cimento resinoso utilizado, os corpos-de-prova foram divididos em sete grupos (10 / grupo). Foram utilizados os seguintes cimentos fotopolimerizáveis: RelyX-Veneer (L-RV), Variolink-Veneer (L-VV) e Variolink-Esthetic (L-VE). Os cimentos de cura dual foram: RelyX Ultimate (D-RU), RelyX-Unicem (D-RC) e Variolink-Esthetics DC (D-VE). O grupo controle consistia apenas de cerâmica. Todos os corpos-de-prova foram termociclados em água por 3.500 ciclos entre 5oC-55oC, com tempos de permanência de 30s em cada banho e um tempo de transferência de 10s entre os banhos. A medição da cor foi feita usando os espectrofotômetros VITA Easyshade e ColorEye. A leitura da cor inicial foi realizada 24 horas após a cimentação. As diferenças na cor (ΔE) do EasyShade e ColorEye antes e depois do EAA foram determinadas e comparadas estatisticamente. As comparações dos grupos foram feitas usando o teste t pareados. O nível de significância foi estabelecido em p <0,05. Resultados: os valores médios de diferenças na cor (ΔE) obtidos no espectrofotômetro EasyShade para cimentos fotopolimerizáveis e duais foram de 0,843 ± 0,89 (L-RV), 4,11 ± 0,69 (L-VV) e 0,833 ± 0,47 (L-VE ); e 2,22 ± 0,64 (D-RU), 3,37 ± 0,83 (D-RC) e 0,38 ± 0,92 (D-VE), respectivamente. os valores médios de diferenças na cor (ΔE) obtidos no espectrofotômetro ColorEye para cimentos fotopolimerizáveis e duais foram de 0,68 ± 0,86 (L-RV), 4,55 ± 0,83 (L-VV) e 2,68 ± 0,26 (L-VE); e 2,06 ± 0,84 (D-RU), 1,8 ± 1,08 (D-RC) e 0,96 ± 0,71 (D-VE), respectivamente. Não houve diferença significativa nos valores médios de ΔE entre os grupos. Conclusão: Os sistemas de combinação de cores VITA Easyshade e ColorEye são comparáveis em termos de eficácia para avaliação de cores de cerâmicas após EAA. (AU)
Assuntos
Técnicas In Vitro , Espectrofotômetros , Materiais Dentários , Facetas DentáriasRESUMO
The objective of this work is to evaluate the influence of accelerated aging test at 41ºC on the germination and seed vigor of Piptadenia moniliformisBenth. Two experiments were conducted. Experiment 1: seeds were submitted to dormancy overcoming treatments. They were immersed into sulfuric acid for 0 (intact seeds), 5, 10, 15, 20 and 25 minutes. Experiment 2: two methods were evaluated for the accelerated aging test at 41ºC, that is, the traditional method and the saturated NaCl solution method with exposure periods of 0, 24, 48, 72 and 96 hours. After each treatment, the seeds were subjected to electrical conductivity and germination tests. Seeds overcame their dormancy when immersed into concentrated sulfuric acid for 5, 10, 15, 20 or 25 minutes, but thegermination was faster when immersed for 20 minutes. The different methods for the accelerated aging test, decreased the germination potential and seed vigor of P. moniliformisafter 24 hours of exposure. Regarding the method used, the saturated solution provided, in general, the best results since the seed water content was lower, thus reducing the proliferation of fungi. The electrical conductivity test showed that, by increasing the aging period regardless of the method, there is an increase in seed deterioration, corroborating with germination results, which showed that the correlation was moderate and negative and indicated that the higher the conductivity, the lower the percentage and the germination speed index. The immersion of seeds of P. moniliformisinto concentrated sulfuric acid for 20 minutes provides a fast and increased germination. The methods for accelerated agingat 41ºC, traditional and saturated solution, can be used as vigor tests to evaluate the physiological quality of seeds.
Assuntos
Envelhecimento , Fabaceae/anatomia & histologia , Fabaceae/fisiologia , Fabaceae/química , Resposta ao Choque TérmicoRESUMO
Chia (Salvia hispanica L.) is a plant native to central and western Mexico and northern Guatemala belonging to the Lamiaceae family, well known for its seeds containing antioxidants, dietary fibers, and α-linolenic acid, which helps to prevent different diseases, such as diabetes, obesity, cancer and Alzheimer. In Brazil, there is not much information about the crop, mainly about the physiological quality of its seeds. Therefore, the objective was to establish vigor testing methods to evaluate the physiological quality of chia seeds. Five seed lots were used, in which germination tests, seedling emergence, emergence velocity index and variations in the electrical conductivity test were carried out. The volume of distilled water was 25 and 50 mL, the number of seeds was 50 and 100, the temperature was 20, 25 and 30 °C, the soaking period was 1, 2, 4, 6, 12, 18 and 24 hours and variations in there was accelerated aging test by the traditional method and saturated NaCl solution, being periods of aging as 24, 48, 72 and 96 hours and temperatures of 38, 41 and 44 °C. It is concluded that both the electrical conductivity test and the accelerated aging test are efficient in evaluating the physiological quality of chia seeds. The electrical conductivity test must be performed with 100 seeds in 50 mL of distilled water, for four hours soaking at 25 or 30 °C; and the accelerated aging test should be performed at 44 °C for 24 hours by the traditional method or 41 °C for 24 hours using saturated NaCl solution.(AU)
Pertencente à família das Lamiáceas, a chia (Salvia hispanica L.) é uma planta originária do centro-oeste do México e norte da Guatemala, muito conhecida por suas sementes conterem antioxidantes, fibras dietéticas e ácido α-linolênico, que auxilia na prevenção de várias doenças, como diabetes, obesidade, câncer e Alzheimer. No Brasil, não há muitas informações a respeito da cultura, principalmente sobre qualidade fisiológica de sementes. Sendo assim, o objetivo foi estabelecer metodologia de testes de vigor para avaliar a qualidade fisiológica de sementes de chia. Foram utilizados cinco lotes de sementes, nos quais foram realizados os testes de germinação, emergência de plântulas, índice de velocidade de emergência e variações no teste de condutividade elétrica, sendo volume de água destilada: 25 e 50 mL, quantidade de sementes: 50 e 100, temperatura: 20, 25 e 30 °C e período de embebição: 1, 2, 4, 6, 12, 18 e 24 horas e variações no teste de envelhecimento acelerado pelo método tradicional e solução saturada de NaCl, sendo períodos de envelhecimento: 24, 48, 72 e 96 horas e temperaturas: 38, 41 e 44 ºC. Conclui-se que tanto o teste de condutividade elétrica quanto o de envelhecimento acelerado são eficientes na avaliação da qualidade fisiológica de sementes de chia. O teste de condutividade elétrica deve ser realizado com 100 sementes em 50 mL de água destilada, pelo período de quatro horas de embebição, à 25 ou 30 °C; e o teste de envelhecimento acelerado deve ser realizado à 44 °C por 24 horas pelo método tradicional ou 41 °C por 24 horas utilizando solução saturada de NaCl.(AU)
Assuntos
Salvia/crescimento & desenvolvimento , Salvia/fisiologia , Sementes/crescimento & desenvolvimento , Sementes/fisiologia , Condutividade Elétrica , GerminaçãoRESUMO
Chia (Salvia hispanica L.) is a plant native to central and western Mexico and northern Guatemala belonging to the Lamiaceae family, well known for its seeds containing antioxidants, dietary fibers, and α-linolenic acid, which helps to prevent different diseases, such as diabetes, obesity, cancer and Alzheimer. In Brazil, there is not much information about the crop, mainly about the physiological quality of its seeds. Therefore, the objective was to establish vigor testing methods to evaluate the physiological quality of chia seeds. Five seed lots were used, in which germination tests, seedling emergence, emergence velocity index and variations in the electrical conductivity test were carried out. The volume of distilled water was 25 and 50 mL, the number of seeds was 50 and 100, the temperature was 20, 25 and 30 °C, the soaking period was 1, 2, 4, 6, 12, 18 and 24 hours and variations in there was accelerated aging test by the traditional method and saturated NaCl solution, being periods of aging as 24, 48, 72 and 96 hours and temperatures of 38, 41 and 44 °C. It is concluded that both the electrical conductivity test and the accelerated aging test are efficient in evaluating the physiological quality of chia seeds. The electrical conductivity test must be performed with 100 seeds in 50 mL of distilled water, for four hours soaking at 25 or 30 °C; and the accelerated aging test should be performed at 44 °C for 24 hours by the traditional method or 41 °C for 24 hours using saturated NaCl solution.
Pertencente à família das Lamiáceas, a chia (Salvia hispanica L.) é uma planta originária do centro-oeste do México e norte da Guatemala, muito conhecida por suas sementes conterem antioxidantes, fibras dietéticas e ácido α-linolênico, que auxilia na prevenção de várias doenças, como diabetes, obesidade, câncer e Alzheimer. No Brasil, não há muitas informações a respeito da cultura, principalmente sobre qualidade fisiológica de sementes. Sendo assim, o objetivo foi estabelecer metodologia de testes de vigor para avaliar a qualidade fisiológica de sementes de chia. Foram utilizados cinco lotes de sementes, nos quais foram realizados os testes de germinação, emergência de plântulas, índice de velocidade de emergência e variações no teste de condutividade elétrica, sendo volume de água destilada: 25 e 50 mL, quantidade de sementes: 50 e 100, temperatura: 20, 25 e 30 °C e período de embebição: 1, 2, 4, 6, 12, 18 e 24 horas e variações no teste de envelhecimento acelerado pelo método tradicional e solução saturada de NaCl, sendo períodos de envelhecimento: 24, 48, 72 e 96 horas e temperaturas: 38, 41 e 44 ºC. Conclui-se que tanto o teste de condutividade elétrica quanto o de envelhecimento acelerado são eficientes na avaliação da qualidade fisiológica de sementes de chia. O teste de condutividade elétrica deve ser realizado com 100 sementes em 50 mL de água destilada, pelo período de quatro horas de embebição, à 25 ou 30 °C; e o teste de envelhecimento acelerado deve ser realizado à 44 °C por 24 horas pelo método tradicional ou 41 °C por 24 horas utilizando solução saturada de NaCl.
Assuntos
Condutividade Elétrica , Germinação , Salvia/crescimento & desenvolvimento , Salvia/fisiologia , Sementes/crescimento & desenvolvimento , Sementes/fisiologiaRESUMO
Multilayer ceramic capacitors (MLCC) are essential components for determining the reliability of electronic components in terms of time to failure. It is known that the reliability of MLCCs depends on their composition, processing, and operating conditions. In this present work, we analyzed the lifetime of three similar X7R type MLCCs based on BaTiO3 by conducting High Accelerated Life Tests (HALT) at temperatures up to 200 °C at 400 V and 600 V. The results were adjusted to an Arrhenius equation, which is a function of the activation energy (Ea) and a voltage stress exponent (n), in order to predict their time to failure. The values of Ea are in the range of 1â»1.45 eV, which has been reported for the thermal failure and dielectric wear out of BaTiO3-based dielectric capacitors. The stress voltage exponent value was in the range of 4â»5. Although the Ea can be associated with a failure mechanism, n only gives an indication of the effect of voltage in the tests. It was possible to associate those values with each type of tested MLCC so that their expected life could be estimated in the range of 400â»600 V.
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Schizophrenia (SZ) is associated with increased somatic morbidity and mortality, in addition to cognitive impairments similar to those seen in normal aging, which may suggest that pathological accelerated aging occurs in SZ. Therefore, we aim to evaluate the relationships of age, telomere length (TL), and CCL11 (aging and inflammatory biomarkers, respectively), gray matter (GM) volume and episodic memory performance in individuals with SZ compared to healthy controls (HC). One hundred twelve participants (48 SZ and 64 HC) underwent clinical and memory assessments, structural MRI, and had their peripheral blood drawn for biomarkers analysis. Comparisons of group means and correlations were performed. Participants with SZ had decreased TL and GM volume, increased CCL11, and worse memory performance compared to HC. In SZ, shorter TL was related to increased CCL11, and both biomarkers were related to reduced GM volume, all of which were related to worse memory performance. Older age was only associated with reduced GM, but longer duration of illness was related with all the aforementioned variables. Younger age of disease onset was associated with increased CCL11 levels and worse memory performance. In HC, there were no significant correlations except between memory and GM. Our results are consistent with the hypothesis of accelerated aging in SZ. These results may indicate that it is not age itself, but the impact of the disease associated with a pathological accelerated aging that leads to impaired outcomes in SZ.
Assuntos
Senilidade Prematura , Quimiocina CCL11/sangue , Disfunção Cognitiva , Substância Cinzenta/patologia , Memória Episódica , Esquizofrenia , Encurtamento do Telômero/fisiologia , Adulto , Senilidade Prematura/metabolismo , Senilidade Prematura/patologia , Senilidade Prematura/fisiopatologia , Biomarcadores , Disfunção Cognitiva/metabolismo , Disfunção Cognitiva/patologia , Disfunção Cognitiva/fisiopatologia , Feminino , Humanos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Esquizofrenia/metabolismo , Esquizofrenia/patologia , Esquizofrenia/fisiopatologiaRESUMO
Schizophrenia (SZ) is associated with broad burden. The clinical manifestations of SZ are related to pathophysiological alterations similar to what is seen in normal aging. Our aim was to evaluate the differences in telomere length (TL), a biomarker of cellular aging, in subjects with SZ (n=36), unaffected siblings (SB, n=36) and healthy controls (HC, n=47). SZ had shorter TL compared to HC, but no difference was found in SB comparing to SZ. These findings indicate that a pathological accelerated aging profile could be present in the course of SZ and further studies are needed to confirm TL as potential endophenotype, especially in at risk populations.
Assuntos
Esquizofrenia/metabolismo , Irmãos , Encurtamento do Telômero , Adolescente , Adulto , Envelhecimento/metabolismo , Análise de Variância , Antipsicóticos/uso terapêutico , Índice de Massa Corporal , Escolaridade , Endofenótipos , Feminino , Humanos , Entrevista Psicológica , Masculino , Pessoa de Meia-Idade , Escalas de Graduação Psiquiátrica , Esquizofrenia/diagnóstico , Esquizofrenia/tratamento farmacológico , Telômero/metabolismo , Adulto JovemRESUMO
OBJECTIVE: To evaluate the influence of 16% carbamide peroxide (CP) containing different thickeners on the physical characteristics of a nanocomposite resin submitted or not to accelerated artificial aging (AAA). MATERIALS AND METHODS: One hundred samples were randomly distributed into two groups (n = 50) according to AAA. Each group was divided into 5 subgroups (n = 10) depending on the bleaching/thickener treatment: CP + carbopol, CP + natrosol, carbopol, natrosol, and no treatment (control). The physical properties tested were color (ΔE), gloss (GU), mean roughness (Ra), and Knoop microhardness (KHN). The resin surface was performed with atomic force microscopy (AFM). STATISTICAL ANALYSIS: The color (variable Δ E) was assessed with two-way analysis of variance (ANOVA) and additionally with Tukey's and Dunnett's tests, the roughness values were submitted to Kruskal-Wallis, Dunn's, and Mann-Whitney's tests. Data on gloss and KHN were submitted to two-way ANOVA and Tukey's test (α = 0.05). RESULTS: Among the physical properties evaluated, CP + carbopol promoted a reduction in composite microhardness only, thus differing statistically from the controls. As for CP + natrosol, such a change was not observed. The aging process reduced all the physical properties, thus differing statistically from the nonaging group. CP + carbopol increased the roughness and decreased the gloss of aged resins, whereas natrosol reduced gloss only, which differed statistically from the controls. CONCLUSIONS: AFM showed evidence of the loss of organic matrix and exposure to load particles in the aged samples. Therefore, the replacement of carbopol with natrosol provided maintenance of the composite microhardness following bleaching. The aging process reduced the physical properties evaluated, and some changes were enhanced by the application of bleaching.
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OBJECTIVES: To evaluate the effect of photoinitiators and reducing agents on cure efficiency and color stability of resin-based composites using different LED wavelengths. METHODS: Model resin-based composites were associated with diphenyl(2,4,6-trimethylbenzoyl) phosphine oxide (TPO), phenylbis(2,4,6-trimethylbenzoyl) phosphine oxide (BAPO) or camphorquinone (CQ) associated with 2-(dimethylamino) ethyl methacrylate (DMAEMA), ethyl 4-(dimethyamino) benzoate (EDMAB) or 4-(N,N-dimethylamino) phenethyl alcohol (DMPOH). A narrow (Smartlite, Dentisply) and a broad spectrum (Bluephase G2, Ivoclar Vivadent) LEDs were used for photo-activation (20 J/cm(2)). Fourier transform infrared spectroscopy (FT-IR) was used to evaluate the cure efficiency for each composite, and CIELab parameters to evaluated color stability (ΔE00) after aging. The UV-vis absorption spectrophotometric analysis of each photoinitiator and reducing agent was determined. Data were analyzed using two-way ANOVA and Tukey's test for multiple comparisons (α=0.05). RESULTS: Higher cure efficiency was found for type-I photoinitiators photo-activated with a broad spectrum light, and for CQ-systems with a narrow band spectrum light, except when combined with an aliphatic amine (DMAEMA). Also, when combined with aromatic amines (EDMAB and DMPOH), similar cure efficiency with both wavelength LEDs was found. TPO had no cure efficiency when light-cured exclusively with a blue narrowband spectrum. CQ-systems presented higher color stability than type-I photoinitiators, especially when combined with DMPOH. CONCLUSIONS: After aging, CQ-based composites became more yellow and BAPO and TPO lighter and less yellow. However, CQ-systems presented higher color stability than type-I photoinitiators, as BAPO- and TPO-, despite their higher cure efficiency when photo-activated with corresponding wavelength range. CLINICAL SIGNIFICANCE: Color matching is initially important, but color change over time will be one of the major reasons for replacing esthetic restorations; despite the less yellowing of these alternative photoinitiators, camphorquinone presented higher color stability.
Assuntos
Bis-Fenol A-Glicidil Metacrilato/química , Cor , Lâmpadas de Polimerização Dentária/classificação , Cura Luminosa de Adesivos Dentários/métodos , Fotoiniciadores Dentários/química , Substâncias Redutoras/química , Resinas Acrílicas/química , Cânfora/análogos & derivados , Cânfora/química , Resinas Compostas/química , Luz , Teste de Materiais , Metacrilatos/química , Fosfinas/química , Poliuretanos/química , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
Objetivou-se com este trabalho avaliar o desempenho de sementes de oito genótipos de Carica papaya L. antes e após o envelhecimento acelerado. Foram avaliadas as características físicas: umidade, comprimento, diâmetro e peso de mil sementes. Sementes não envelhecidas (controle) e sementes submetidas ao envelhecimento acelerado (43 ºC por 72 h) foram semeadas em rolo de papel germitest e mantidas em BOD a 20-30 ºC. Foram avaliadas: porcentagem e frequência de germinação, índice de velocidade de germinação, tempo médio de germinação e porcentagem de plantas normais. Após 30 dias da semeadura foram avaliados comprimentos da parte aérea e da raiz, massas fresca e seca das plântulas. Em sementes não envelhecidas, o genótipo Caliman 01 apresentou maiores porcentagem, velocidade e homogeneidade de germinação, e maior porcentagem de plântulas normais. As sementes do Híbrido JS12 x Waimanalo apresentaram maior vigor após o envelhecimento (84% de germinação e 93% de plântulas normais). A análise de diversidade identificou como dissimilares os genótipos Caliman 01 em sementes não envelhecidas e o Híbrido JS12 x Waimanalo em sementes após o envelhecimento. Estes genótipos apresentam maior desempenho com base nas características físicas e fisiológicas das sementes e na análise de diversidade nas respectivas condições. O tempo médio de germinação é a característica que mais contribui para a separação dos genótipos em sementes não envelhecidas enquanto, em sementes envelhecidas, é o comprimento de raiz.
The objective of this study was to evaluate the seeds performance in eight Carica papaya genotypes, before and after accelerated aging test. We evaluated the seeds physical characteristics: humidity, length, diameter and weight of thousand seeds. Not aging seeds (control) and seeds subjected to accelerated aging test (43 º C for 72 h) were germinates in paper roll germitest and maintained in BOD at 20-30 º C. From the daily count, was evaluated the germination percentage and frequency, the speed of germination index, germination time, and the normal plants percentage. After 30 days of culture were evaluated lengths of shoot and root, fresh and dry weight of seedlings. In control seeds, the Caliman 01 genotype had higher percentage, speed and uniformity of germination, and higher normal seedlings percentage. Seeds of the Hybrid JS12 x Waimanalo genotype showed higher vigor after aging (84% of germination and 93% of normal seedlings). The diversity analysis identified as dissimilar the Caliman 01 genotype in control seeds and the Hybrid JS12 x Waimanalo genotype in after aging seeds. This genotypes have higher performance based on assessments of physical and physiological quality of seeds and diversity analysis in this conditions. The germination time was the feature that most contributes to the separation of genotypes in control seeds, and the root length in after aging seeds.
Assuntos
Sementes , Análise Multivariada , Germinação , CaricaRESUMO
Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.(AU)
O teste de germinação é utilizado na avaliação da qualidade fisiológica de sementes; porém, devido à sua condução em condições ideais, não tem se mostrado suficiente para este propósito. Nesse sentido, há a possibilidade de recorrer aos testes de vigor, conquanto, a ausência de metodologias padronizadas tem dificultado a sua aplicabilidade e reprodutibilidade. Assim, o trabalho teve como objetivo determinar as metodologias dos testes de germinação, envelhecimento acelerado e condutividade elétrica, a fim de avaliar a qualidade fisiológica de sementes de pitaia. Para o estudo foram utilizadas sementes de frutos maduros de Hylocereus undatus. O delineamento experimental foi o inteiramente casualizado, com quatro repetições. A qualidade fisiológica das sementes foi avaliada pelos testes de germinação, envelhecimento acelerado e condutividade elétrica, além da determinação do índice de velocidade de germinação (IVG) e do tempo médio de germinação (TMG), ambos, em conjunto aos testes de germinação e envelhecimento acelerado. Para a análise estatística foram feitos ajustes de modelos de regressão e cálculo do coeficiente de correlação de Pearson (p < 0,05). O teste de germinação em sementes de H. undatus pode ser conduzido a 25 °C, tendo em vista alcançar maior IVG e menor TMG. O envelhecimento acelerado pode ser conduzido a 43 °C por 48 h, já que a combinação destes fatores é favorável à expressão do vigor das sementes, alcançando o ponto de máximo e de mínimo do IVG e do TMG, respectivamente, além da economia de tempo na sua condução. O teste de condutividade elétrica pode ser conduzido com 25 sementes, na temperatura de 30 °C e volume de água de 10 mL, já que nesta condição, há menor interferência de fatores externos ao conteúdo de lixiviados na solução.(AU)
Assuntos
Germinação , Cactaceae/citologia , Cactaceae/fisiologia , Condutividade ElétricaRESUMO
Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.
O teste de germinação é utilizado na avaliação da qualidade fisiológica de sementes; porém, devido à sua condução em condições ideais, não tem se mostrado suficiente para este propósito. Nesse sentido, há a possibilidade de recorrer aos testes de vigor, conquanto, a ausência de metodologias padronizadas tem dificultado a sua aplicabilidade e reprodutibilidade. Assim, o trabalho teve como objetivo determinar as metodologias dos testes de germinação, envelhecimento acelerado e condutividade elétrica, a fim de avaliar a qualidade fisiológica de sementes de pitaia. Para o estudo foram utilizadas sementes de frutos maduros de Hylocereus undatus. O delineamento experimental foi o inteiramente casualizado, com quatro repetições. A qualidade fisiológica das sementes foi avaliada pelos testes de germinação, envelhecimento acelerado e condutividade elétrica, além da determinação do índice de velocidade de germinação (IVG) e do tempo médio de germinação (TMG), ambos, em conjunto aos testes de germinação e envelhecimento acelerado. Para a análise estatística foram feitos ajustes de modelos de regressão e cálculo do coeficiente de correlação de Pearson (p < 0,05). O teste de germinação em sementes de H. undatus pode ser conduzido a 25 °C, tendo em vista alcançar maior IVG e menor TMG. O envelhecimento acelerado pode ser conduzido a 43 °C por 48 h, já que a combinação destes fatores é favorável à expressão do vigor das sementes, alcançando o ponto de máximo e de mínimo do IVG e do TMG, respectivamente, além da economia de tempo na sua condução. O teste de condutividade elétrica pode ser conduzido com 25 sementes, na temperatura de 30 °C e volume de água de 10 mL, já que nesta condição, há menor interferência de fatores externos ao conteúdo de lixiviados na solução.
Assuntos
Cactaceae/citologia , Cactaceae/fisiologia , Condutividade Elétrica , GerminaçãoRESUMO
Populações de cenoura com sementes de maior qualidade fisiológica e potencial de armazenamento são de interesse para as empresas de sementes e horticultores. Assim, verificou-se a eficiência de alguns testes para a seleção de populações de cenoura com maior vigor e longevidade das sementes. Sementes de 50 progênies de meio-irmãos de cenoura tipo Brasília foram avaliadas quanto à massa de cem sementes, primeira contagem de germinação, germinação, dormência, envelhecimento acelerado com água e com solução saturada de NaCl. As sementes foram armazenadas com teor de água de 6,1±0,3% em embalagem hermética nas temperaturas de 15 e 25oC por 12 meses e a germinação foi avaliada trimestralmente. Os dados experimentais foram avaliados quanto à variância e correlações fenotípicas, genotípicas e ambientais; herdabilidade; coeficiente de variação genética e ganho por seleção. A seleção baseada na massa não deve ser utilizada, pois aumentaria a ocorrência de dormência nas sementes da geração seguinte. Os testes da primeira contagem, de germinação e de envelhecimento acelerado com água ou solução salina saturada podem ser utilizados para selecionar populações de cenoura com sementes de maior vigor e longevidade. O ganho genético estimado com a seleção para a germinação após 12 meses de armazenamento a 25°C foi de 14%.(AU)
Populations of carrot seeds with superior physiological quality and storage potential are of interest to seed companies and growers. Thus, we verified the efficiency of some tests for the selection of carrot populations with greater vigor and longevity of seeds. Seeds of 50 carrots progenies of different half-brothers from Brasilia cultivar were evaluated for the mass of one hundred seeds, the first count, germination, dormancy, accelerated aging with water and saturated NaCl solution. The seeds were stored at moisture contents of 6.1±0.3% in hermetic packaging at temperatures of 15 and 25°C for 12 months and germination was evaluated quarterly. The experimental data were evaluated for variance and phenotypic, genotypic and environmental heritability, coefficient of variation and genetic gain from selection. Selection based on the mass should not be used because it would increase the occurrence of dormancy in seeds of the next generation. The test of the first count, germination and accelerated aging in water or saline solution saturated may be used to select populations of carrot seeds of higher vigor and longevity. The estimated gain genetic selection for germination after 12 months storage at 25°C was 14%.(AU)
Assuntos
Daucus carota , Sementes , GerminaçãoRESUMO
Populações de cenoura com sementes de maior qualidade fisiológica e potencial de armazenamento são de interesse para as empresas de sementes e horticultores. Assim, verificou-se a eficiência de alguns testes para a seleção de populações de cenoura com maior vigor e longevidade das sementes. Sementes de 50 progênies de meio-irmãos de cenoura tipo Brasília foram avaliadas quanto à massa de cem sementes, primeira contagem de germinação, germinação, dormência, envelhecimento acelerado com água e com solução saturada de NaCl. As sementes foram armazenadas com teor de água de 6,1±0,3% em embalagem hermética nas temperaturas de 15 e 25oC por 12 meses e a germinação foi avaliada trimestralmente. Os dados experimentais foram avaliados quanto à variância e correlações fenotípicas, genotípicas e ambientais; herdabilidade; coeficiente de variação genética e ganho por seleção. A seleção baseada na massa não deve ser utilizada, pois aumentaria a ocorrência de dormência nas sementes da geração seguinte. Os testes da primeira contagem, de germinação e de envelhecimento acelerado com água ou solução salina saturada podem ser utilizados para selecionar populações de cenoura com sementes de maior vigor e longevidade. O ganho genético estimado com a seleção para a germinação após 12 meses de armazenamento a 25°C foi de 14%.
Populations of carrot seeds with superior physiological quality and storage potential are of interest to seed companies and growers. Thus, we verified the efficiency of some tests for the selection of carrot populations with greater vigor and longevity of seeds. Seeds of 50 carrots progenies of different half-brothers from Brasilia cultivar were evaluated for the mass of one hundred seeds, the first count, germination, dormancy, accelerated aging with water and saturated NaCl solution. The seeds were stored at moisture contents of 6.1±0.3% in hermetic packaging at temperatures of 15 and 25°C for 12 months and germination was evaluated quarterly. The experimental data were evaluated for variance and phenotypic, genotypic and environmental heritability, coefficient of variation and genetic gain from selection. Selection based on the mass should not be used because it would increase the occurrence of dormancy in seeds of the next generation. The test of the first count, germination and accelerated aging in water or saline solution saturated may be used to select populations of carrot seeds of higher vigor and longevity. The estimated gain genetic selection for germination after 12 months storage at 25°C was 14%.
Assuntos
Daucus carota , Germinação , SementesRESUMO
This study evaluated the mechanical, thermal, and morphological characteristics of different classifications of dental composites as a function of the material condition (new, aged and expired). Specimens were obtained according to these factors: Composites: Filtek P60, Filtek Z250, Filtek Z350XT, and Filtek Silorane; and Material conditions: new, aged, and expired. The syringe composites underwent an accelerated aging protocol (Arrhenius model). The flexural strength (FS) and flexural modulus (E) were obtained. The thermogravimetric analysis (TGA) and differential thermal analysis (DTA) were also performed and the glass transition temperature (Tg) and the weight loss calculated. Topographic analysis of the composites was performed under SEM. The material conditions influenced the mechanical properties of the composites. The silorane composite exhibited a characteristic thermal behavior different from that of the methacrylates. In general, the Tg increased after the accelerated aging protocol and decreased for expired ones, compared to the new composites. A significant increase in FS of Filtek Z350XT after aging was accompanied by an increase in the Tg. The filler packings were in accordance with the manufacture׳s information. The topographic aspects of the composites were modified as a function of the material condition. The mechanical properties of the composites following a simplified protocol of accelerated aging varied as a function of the expiration date. The silorane composite presented a characteristic thermal behavior. Although the dental manufacturers may not be able to control variables as storage temperature and transportation conditions, these effects on the composite clinical performance can be minimized if properly considered.