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Leopardus geoffroyi (Geoffroy's cat) is a neotropical feline considered globally threatened. In Brazil, it occurs exclusively in the Pampa biome. Its predatory habits contribute to the infection, dispersion, and continuation of the life cycle of various pathogens, including helminths, within ecosystems. However, few studies involving cestodes in wild felines are found in the literature, especially in Brazil. Therefore, we aimed to report the first case of parasitism by Hydatigera taeniaeformis in L. geoffroyi. The helminths were found in the small intestine of the necropsied feline. Specimens were analyzed morphometrically and subjected to molecular analyses for taxonomic identification. The molecular phylogeny based on the analysis of the mitochondrial gene (COX1) allowed the identification of these parasites. Thus, this is the first description of H. taeniaeformis parasitizing L. geoffroyi in Brazil. Consequently, the number of known host species parasitized by this helminth in the country and the world is increased. Additionally, a new molecular sequence is being provided, contributing to the knowledge of Hydatigera in South America.

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Diseases such as those caused by feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) represent health problems for cats. Feline leishmaniasis (FL) has been reported in several cities across the country. The objective was to carry out a clinical-epidemiological and laboratory study of FIV, FeLV and FL in cats from shelters in Dourados, Mato Grosso do Sul, Brazil. Blood samples and swabs from the conjunctival and nasal mucosa were obtained from 75 cats, from four animal shelters. Serology for FIV and FeLV was performed. For Leishmania, polymerase chain reaction (PCR) was performed on blood, conjunctiva and nasal mucosa. In the immunochromatographic serological test, seven cats tested positive for FIV and none for FeLV. No samples was positive in PCR for Leishmania. The study showed that despite the presence of human and canine leishmaniasis in the studied region, Leishmania spp. were absent in the cats studied. To avoid an increase in contagion in shelters, it is essential isolate cats with FIV.

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Sporotrichosis is a traumatic mycosis affecting the skin or subcutaneous tissues caused by Sporothrix dimorphic fungus. The fungal complex includes several pathogenic species, out of which S. brasiliensis and S. schenckii are predominant in Brazil. In Mato Grosso do Sul (MS) state, the first human and animal cases were reported in 2016 in Corumbá and Ladário cities. Accordingly, we present the first occurrences of feline sporotrichosis detected in the state capital Campo Grande, MS, by the Zoonoses Control Service (ZCS) of the Municipal Public Health Department. The study included four allochthonous cases of feline sporotrichosis originating from Corumbá, MS, attended by the ZCS. All four cats presented classical clinical signs of sporotrichosis, as ulcerative nodular cutaneous lesions. Three slides tested positive by direct microscopy and PCR, followed by Sanger sequencing confirmed Sporothrix brasiliensis in two samples. The initial suspicion and diagnosis of feline sporotrichosis at the ZCS highlights the importance of accurate surveillance of sporotrichosis in non-endemic areas to enhance the capacity to prevent, detect and respond to emerging diseases in Campo Grande.

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Hypostomus soniae is a small sized armored catfish endemic to the Tapajos River basin and ranked as one of the most exploited ornamental fish in the Santarem export marketplace. This study aims to evaluate distributional patterns of Hypostomus soniae and contribute to the species conservation in the face of development of the ornamental fish trade in the Amazon region. We compiled data associated with geographic coordinates in public repositories, supplemented with original field records. We compared our data to published records in the literature and museum collections to check for accuracy. To investigate the fishery and commercialization of H. soniae, we conducted interviews with ornamental fish stakeholders from the local trade. We also made direct observations in the fishing sites and export facilities in Santarem. A cluster analysis of the geolocation data was carried out to explore the spatial distribution patterns. The volume of captures and exportation of H. soniae decreased during the period 2020-2023. The occurrence of H. soniae was associated with annual rainfall ranging from 2,000 mm to 2,500 mm and concentrated in two municipalities of the State of Mato Grosso and two of the Para State. The species distribution area has been threatened, unfortunately, by fishermen who do not respect the laws that support artisanal fishing in the Amazon.

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Foi atendido no Hospital Veterinária da Universidade Federal de Mato Grosso (HOVET-UFMT) - Campus Cuiabá, um gato palheiro (Leopardus braccatus), macho, filhote e pesando 1,8kg. Após avaliação clínica e exames complementares diagnosticou-se presença de corpo estranho solido gástrico e intestinal. Como medicação pré-anestésica optou-se pela associação de cetamina (1mg/kg) e midazolam (0,2mg/kg), seguiu-se com a indução com propofol (à efeito) e manutenção anestésica por meio do fornecimento de isofluorano. Como técnica adicional utilizou-se epidural, com uma associação de lidocaína (4,5mg/kg) e morfina (0,1mg/kg). Durante o procedimento anestésico notou-se estabilidade das variáveis cardiovasculares e respiratórias, além de recuperação satisfatória ao final do procedimento.

Was attended to in the Veterinary Hospital of the Federal University of Mato Grosso (HOVET-UFMT) - Campus Cuiabá, a pantanal cat (Leopardus braccatus), male, puppy and weighing 1.8 kg. After clinical evaluation and complementary exams, the presence of a solid gastric and intestinal foreign body was diagnosed. As pre-anesthetic medication, the association of ketamine (1mg/kg) and midazolam (0.2mg/kg) was chosen, followed by induction with propofol (for effect) and anesthetic maintenance by supplying isoflurane. As an additional technique, an epidural was used, with an association of lidocaine (4.5mg/kg) and morphine (0.1mg/kg). During the anesthetic procedure, stability of cardiovascular and respiratory variables was observed, in addition to satisfactory recovery at the end of the procedure.

Ingresó en el Hospital Veterinario de la Universidad Federal de Mato Grosso (HOVET-UFMT) · Campus Cuiabá, un gato palheiro (Leopardus braccatus), macho, cachorro y con un peso de 1,8 kg. Luego de evaluación clínica y exámenes complementarios se diagnosticó la presencia de cuerpo extraño sólido gástrico e intestinal. Como medicación preanestésica se optó por la asociación de ketamina (1 mg/kg) y midazolam (0,2 mg/kg), seguida de inducción con propofol (por efecto) y mantenimiento anestésico mediante suministro de isoflurano. Como técnica adicional se utilizó epidural, con asociación de lidocaína (4,5 mg/kg) y morfina (0,1 mg/kg). Durante el procedimiento anestésico se observó estabilidad de variables cardiovasculares y respiratorias, además de recuperación satisfactoria al final del procedimiento.

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Helicobacter is a genus of Gram-negative bacteria that colonizes the stomach of humans and dogs, among other mammals. The most frequent species found in the gastric mucosa of man is H. pylori, however, there are other species of Helicobacter in the stomach of humans, such as H. suis, H. bizzozeronii, H. felis, H. salomonis and H. heilmannii sensu stricto, named non H. pylori Helicobacter (NHPH) some of which also colonize the dog's stomach. The detection of NHPH in the stomach mucosa of humans with gastric disease, and in a high percentage of dogs, suggests the possibility that these animals play an important role in the pathogenesis and transmission of infection to man, postulating the path of transmission oral-oral or fecal-oral. The aim of this study was to determine whether people with signs of chronic gastritis who have Helicobacter spp. and who own dogs as pets, share the same species with them. The presence of Helicobacter spp. in gastric samples of 30 people with clinical signs of chronic gastritis and histopathological diagnosis of gastric infection with Helicobacter spp. was analyzed by PCR. The presence of Helicobacter spp. was also studied in the dogs of these people. Then, the species of Helicobacter presented was determined in both. In human gastric samples, H. pylori was the only species found, while in dogs, samples were H. bizzozeronii, H. felis, H. salomonis and H. heilmannii. In this work the same species were not found in dogs and their owners.

Helicobacter es un género bacteriano gram negativo que coloniza el estómago de humanos y perros, entre otros mamíferos. En la mucosa gástrica del hombre la especie más frecuentemente hallada es H. pylori, sin embargo, puede albergar otras especies, como H. suis, H. bizzozeronii, H. felis, H. salomonis y H. heilmannii sensu stricto, denominadas Helicobacter no H. pylori (NHPH), algunas de las cuales colonizan también el estómago del perro. La detección de especies NHPH en la mucosa del estómago de seres humanos con enfermedad gástrica y en un alto porcentaje de perros como mascotas, sugiere la posibilidad de que estos animales jueguen un rol importante en la patogenia y transmisión de la infección al hombre, postulándose la vía de transmisión oral-oral o bien fecal-oral. El objetivo de este trabajo fue determinar si individuos con signos de gastritis crónica que tienen Helicobacter spp. y que poseen perros como mascotas, comparten con ellos las mismas especies. Para ello se estudió por PCR la presencia de Helicobacter spp. en muestras gástricas de 30 pacientes con signos clínicos de gastritis crónica y diagnóstico histopatológico de infección gástrica con Helicobacter spp. También se estudió la presencia de estas bacterias en sus perros para determinar si existe correspondencia en las especies presentes en ambos. La única especie encontrada en humanos fue H. pylori, mientras que en los perros se encontraron H. bizzozeronii, H. felis, H. salomonis y H. heilmannii. En este trabajo no se encontraron las mismas especies en los perros y en sus propietarios.

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Quantitative abilities are well described in many species and in diverse life situations, including in the adult domestic cat. However, such abilities have been much less studied during ontogeny. In the present study we examined spontaneous quantity discrimination by pre-weaning age kittens in two-way food choice experiments. In Experiment 1, 26 kittens performed 12 trials with different ratios between the number of same-size food items. In Experiment 2, 24 other kittens performed eight trials with different ratios between the size of two food items. We found, in general, that the kittens discriminated between the different amounts of food and spontaneously chose the larger one, but that their choice was influenced by the ratio of difference. The kittens in Experiment 1 chose the larger number of same-size food items if the ratio was smaller than 0.4 and in Experiment 2 they chose the larger pieces of food if the ratio between the items was smaller than 0.5. Because the kittens' choice was not influenced by the absolute number of food items or the numerical difference between them in Experiment 1, it suggests that their cognitive performance relied on an analog magnitude system rather than on an object file system during the quantity discrimination tasks. We discuss our results considering the ecological and social background of cats and compare it with the performance of previously studied species.

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Abstract Anamnesis: A polytraumatized wild oncilla (Leopardus tigrinus) was admitted to Unidad de Rehabilitación y Rescate de Animales Silvestres (URRAS) veterinary clinic of Universidad Nacional de Colombia. Despite the medical efforts, the animal died six hours later. Clinical and laboratory findings: Necropsy examination revealed multiple white nodules of about 2 mm in diameter distributed on the visceral surface of lung caudal lobes. Histopathology revealed lymphoplasmacytic interstitial pneumonia with multiple coiled larvae of metastrongyloid nematodes in alveoli and bronchioles. First-stage Aelurostrongylus abstrusus nematode was identified in a fecal sample using the Ritchie copromicroscopic technique. Conclusion: This study reports an incidental severe pulmonary parasitism caused by A. abstrusus in a wild Oncilla (L. tigrinus). This is the first report of A. abstrusus infection associated with pathological lesions in a L. tigrinus from Colombia.

Resumen Anamnesis: Un tigrillo silvestre (Leopardus tigrinus) politraumatizado fue ingresado en la clínica veterinaria Unidad de Rehabilitación y Rescate de Animales Silvestres (URRAS) de la Universidad Nacional de Colombia, donde posteriormente falleció. Hallazgos clínicos y de laboratorio: el examen de necropsia reveló múltiples nódulos blancos de aproximadamente 2 mm de diámetro, distribuidos en la superficie visceral de los lóbulos caudales del pulmón. La histopatología reveló neumonía intersticial linfoplasmocítica, con múltiples larvas enrolladas de nematodos metastrongiloides en alvéolos y bronquiolos. En la muestra fecal postmortem se identificó el nematodo Aelurostrongylus abstrusus en primera etapa por la técnica copromicroscópica de Ritchie. Conclusión: Este estudio reporta un parasitismo pulmonar severo incidental causado por A. abstrusus en un tigrillo silvestre (L. tigrinus). Este es el primer reporte de infección por A. abstrusus asociada con lesiones patológicas en un L. tigrinus en Colombia.

Resumo Anamnese: Um Maracajá (Leopardus tigrinus) selvagem de vida livre foi internado na clínica veterinária Unidad de Rehabilitación y Rescate de Animales Silvestres (URRAS) da Universidad Nacional de Colombia devido a politraumatismo e o animal morreu. Achados clínicos e laboratoriais: O exame de necropsia revelou múltiplos nódulos brancos com cerca de 2 mm de diâmetro distribuídos na superfície visceral do pulmão dos lobos caudais. A histopatologia revelou pneumonia intersticial linfoplasmocitária com múltiplas larvas enroladas de nematóides metastrongilóides em alvéolos e bronquíolos. A amostra fecal post mortem para a técnica copromicroscópica de Ritchie permitiu a identificação do nematóide Aelurostrongylus abstrusus de primeiro estágio. Conclusão: Este estudo relata um grave parasitismo pulmonar incidental causado por A. abstrusus em um Maracajá selvagem de vida livre (L. tigrinus) da Colômbia. Este é o primeiro relato de infecção por A. abstrusus associada a lesões patológicas em L. tigrinus da Colômbia.

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Fleas and ticks represent the two main groups of ectoparasites that infest companion animals. In particular, the flea Ctenocephalides felis felis and several members of the Rhipicephalus sanguineus complex are the main vectors of a wide range of pathogens on the American continent. They are competent vectors for several members of the genus Rickettsia, which encompass at least 15 pathogenic obligate intracellular bacteria that colonize the endothelial cells of vertebrates. In Mexico, 10 species of Rickettsia belonging to three groups have been detected in six species of ectoparasites from dogs in 9 of the 32 states of the country. However, in some larger regions of the country, active epidemiological surveillance has not been carried out. For this reason, the aim of this study was to identify the presence of members of the genus Rickettsia in fleas and ticks of dogs and cats in the state of Puebla, Mexico. A cross-sectional study was carried out to collect ectoparasites of dogs and cats during August to November 2019. Samples were fixed in 70% ethanol and examined to identify the presence of Rickettsia DNA by the amplification and sequencing of specific fragments of the gltA and ompB genes using conventional PCR. The recovered sequences were compared with those deposited in GenBank, and phylogenetic analyses were carried out to identify the position of the pathogens detected with respect to the valid species previously reported worldwide. Additionally, ecological parameters of the ectoparasite infestations were also calculated. We recovered 196 ectoparasites belonging to two species, 33 C. felis felis and 163 R. sanguineus s.l. (Rhipicephalus linnaei), parasitizing 46 hosts (42 dogs and 4 cats) in 11 localities of the state of Puebla. We detected the presence of Rickettsia felis in three pools of C. felis felis, and five from R. sanguineus s.l. Our work provides the first record of R. felis in hard ticks of Mexico and Central America, with new collection localities for this pathogen in central Mexico.

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ABSTRACT: We report a case of a free-ranging five-month wildcat with bilateral hind limbs paralysis since birth due to a segmental lumbar spinal cord aplasia. The species confirmation of the southern tiger cat (Leopardus guttulus) was determined by genetic sequencing. This southern tiger cat native to Brazil had autophagy in both pelvic limbs during the initial phase of hospitalization, followed by a right tibial fracture with bone exposition. Euthanasia was chosen due to animal welfare and submitted for postmortem examination. Grossly, there was an 8.5cm in-length segmental interruption of the spinal cord between the third and fifth lumbar vertebrae, with a lack of spinal cord tissue and collapsed associated dura mater. Microscopically, the representative sections of the L3 to L5 spinal cord had only .an irregular trace of gray matter adhered to the meninges (lumbar spinal cord aplasia) In the region of L6, a focally extensive, cystic, and well-defined tubular cavitation was noted dorsally to the central canal, replacing and compressing the adjacent nervous tissue (syringomyelia). Metagenomics examination did not detect any virus responsible for the presented spinal cord malformations. This seems to be the first description of segmental spinal cord aplasia reported in a wild feline.

RESUMO: O objetivo do presente relato foi descrever um caso de aplasia segmentar da medula espinhal lombar em um felino silvestre, com aproximadamente cinco meses, resgatado de seu ambiente natural, apresentando paralisia bilateral dos membros posteriores. A espécie gato-do-mato-pequeno (Leopardus guttulus) foi determinada por sequenciamento genético. Após curto período de hospitalização, iniciou autofagia de ambos os membros pélvicos, seguido de fratura com exposição óssea. Optou-se pela eutanásia e o cadáver foi encaminhado ao Setor de Anatomia Patológica da Universidade Federal Rural do Rio de Janeiro para necropsia. Macroscopicamente, havia uma interrupção segmentar grave da medula espinhal entre a terceira e a quinta vértebras lombares, medindo 8,5cm de comprimento, com resquícios de tecido nervoso e com meninges colapsadas. Ao exame histológico, em seções da medula espinhal na região de L3 a L5, .havia apenas vestígio de tecido nervoso aderido às meninges, morfologicamente compatível com substância cinzenta (aplasia de medula espinhal lombar). Na região de L6 notou-se áreas multifocais com cavitações tubulares, císticas e bem delimitadas, dorsalmente ao canal central substituindo e comprimindo o tecido nervoso adjacente (siringomielia). O exame de metagenômica não detectou qualquer vírus responsável pelas malformações da medula espinhal. Com base no histórico, sinais clínicos, necropsia e achados histológicos, o diagnóstico de aplasia segmentar grave com siringomielia foi estabelecido em um L. guttulus.

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Nutritional Secondary Hyperparathyroidism (NSH) is a metabolic disorder that mainly affects young animals. This disease causes imbalance between the levels of calcium and phosphorus, culminating in bone changes. Wild animals raised in captivity can develop the disease when they are given an inadequate diet. The aim of this study is to report the clinical findings, the complementary exams and the therapeutics utilized in a Leopardus geoffroyi (Geoffroy's cat) cub originated from captivity with NSH. The animal went through complementary imaging exams and biochemical evaluation that respectively revealed generalized osteopenia and pathological fracture of the right femur, reduction of serum calcium level and elevation of serum phosphorus level. The diagnosis of NSH was established by associating the history, clinical signs, and findings of ancillary exams. The treatment was based on diet correction, mineral and vitaminic supplementation and sun exposure. The NSH diagnosis was stablished by associating history, clinical signs, imaging exams findings and serum biochemicals. The early identification of this disease is of paramount importance, because in this way corrections can be made in food management, in order to provide successful clinical recovery and promote quality of life for wild captive animals.(AU)

O hiperparatireoidismo secundário nutricional (HSN) é uma desordem metabólica que acomete principalmente animais jovens. Essa doença cursa com desequilíbrio entre os níveis de cálcio e fósforo, culminando com alterações ósseas. Animais silvestres quando criados em cativeiro podem desenvolver a doença quando recebem uma dieta inadequada. O obje-tivo deste estudo é relatar os achados clínicos, os exames complementares e a terapêutica utilizada em um filhote de Leopardus geoffroyi (gato-do-mato-grande)proveniente de cativeiro com HSN. O animal passou por exames complementares de imagem e avaliação bioquímica que revelaram respectivamente osteopenia generalizada e fratura patológica de fêmur direito, redução dos níveis séricos de cálcio e elevação do fósforo. O diagnóstico de HSN foi estabelecido associando o histórico, sinais clínicos e achados dos exames complementares. O tratamento foi baseado na correção da dieta, suplementação mineral e vitamínica e exposição solar. A identificação precoce dessa doença é de suma importância, pois dessa forma podem ser feitas correções no manejo alimentar, afim de proporcionar a recuperação clínica com êxito e promover qualidade de vida para os animais selva-gens de cativeiro.(AU)

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Feline piroplasmids include the genera Babesia spp., Cytauxzoon spp., and Theileria spp. In Brazil, there are few reports regarding these hemoprotozoans; however, clinicopathological and molecular data are scarce. This study aimed to characterize the clinical relevance of these parasites through hematological, biochemical, and molecular approaches. For this purpose, 166 cats from Brasilia, Federal District, Midwestern Brazil, were screened using a quantitative polymerase chain reaction (qPCR) for piroplasmids based on the LSU4 mitochondrial gene, which resulted in an overall prevalence of 36/166 (21.7%). Twelve of 166 samples (7.2%) were positive for C. felis, while 19/166 (11.4%) were positive for Babesia vogeli. No samples tested positive for Theileria spp. Babesia vogeli and Cytauxzoon spp. LSU4 sequences showed identities of 97-100% and 99.3%, respectively, to US isolates. The hematological and biochemical findings did not differ significantly between the cats that tested positive and negative for piroplasmids. Although the lack of abnormalities in clinical and laboratory parameters does not eliminate the possibility that these cats were sick and recovered, it may suggest that the Brazilian strain of Cytauxzoon spp. is not as pathogenic as that from the USA, despite the high molecular identity with North American isolates.

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Among the Ctenocephalides felis felis-borne pathogens, Bartonella henselae, the main aetiological agent of cat scratch disease (CSD), is of increasing comparative biomedical importance. Despite the importance of B. henselae as an emergent pathogen, prevention of the diseases caused by this agent in cats, dogs and humans mostly relies on the use of ectoparasiticides. A vaccine targeting both flea fitness and pathogen competence is an attractive choice requiring the identification of flea proteins/metabolites with a dual effect. Even though recent developments in vector and pathogen -omics have advanced the understanding of the genetic factors and molecular pathways involved at the tick-pathogen interface, leading to discovery of candidate protective antigens, only a few studies have focused on the interaction between fleas and flea-borne pathogens. Taking into account the period of time needed for B. henselae replication in flea digestive tract, the present study investigated flea-differentially abundant proteins (FDAP) in unfed fleas, fleas fed on uninfected cats, and fleas fed on B. henselae-infected cats at 24 hours and 9 days after the beginning of blood feeding. Proteomics approaches were designed and implemented to interrogate differentially expressed proteins, so as to gain a better understanding of proteomic changes associated with the initial B. henselae transmission period (24 hour timepoint) and a subsequent time point 9 days after blood ingestion and flea infection. As a result, serine proteases, ribosomal proteins, proteasome subunit α-type, juvenile hormone epoxide hydrolase 1, vitellogenin C, allantoinase, phosphoenolpyruvate carboxykinase, succinic semialdehyde dehydrogenase, glycinamide ribotide transformylase, secreted salivary acid phosphatase had high abundance in response of C. felis blood feeding and/or infection by B. henselae. In contrast, high abundance of serpin-1, arginine kinase, ribosomal proteins, peritrophin-like protein, and FS-H/FSI antigen family member 3 was strongly associated with unfed cat fleas. Findings from this study provide insights into proteomic response of cat fleas to B. henselae infected and uninfected blood meal, as well as C. felis response to invading B. henselae over an infection time course, thus helping understand the complex interactions between cat fleas and B. henselae at protein levels.

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Mycoplasma hemofelis is the most pathogenic hemoplasma species that affect cats. M. hemofelis may cause an acute infection that leads to hemolytic anaemia. The objective of this study was to detect and to quantify the load of M. hemofelis in cats by conventional polymerase chain reaction (PCR) and by quantitative PCR (qPCR) and to describe the possible hematological changes. M. hemofelis DNA was detected in 28.6% of the randomly selected cats (42 of 147) attended at the Veterinary Hospital of the Federal University of Mato Grosso, Brazil. The agreement between conventional PCR and qPCR was substantive (k 0.6). Females were twice as likely to acquire infection as males (odds ratio, 2.31). There was no statistically significant association (P > .05) and little/no correlation between the hematological parameters and the average of bacterial load. The results indicate that M. hemofelis infection is not related to clinical signs and bacterial blood load in cats. The agreement between conventional and quantitative PCR made it possible to detect infection by M. hemofelis in a larger number of cats.

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Fleas are important in public health due to their role as parasites and vectors of pathogens, including Rickettsia. The aim of this study was to evaluate the diversity, abundance and prevalence of fleas and the presence of Rickettsia in the trifinio of north-east Argentina. Fleas from household and synanthropic animals were obtained from urban and periurban areas. They were taxonomically identified and samples of 227 fleas in 86 pools were analysed by polymerase chain reaction targeting the gltA and ompB genes of Rickettsia spp. The study revealed that Ctenocephalides felis felis was dominant on dogs, cats and opossums, with higher prevalence in the periurban area. The Shannon-Wiener and Morisita-Horn indices expressed differences in the diversity and similarity values of the absolute abundances of the species between the areas compared. DNA amplifications revealed 30.8% C. f. felis pools positive for Rickettsia spp. Phylogenetic analysis showed that the haplotype obtained was identical to Rickettsia asembonensis from Peru and Brazil. This is the first detection in Argentina of R. asembonensis that infects C. f. felis, and we emphasize the importance of conducting research from a 'One Health' perspective on the role of opossums and rodents in the integration of the transmission cycles of rickettsial bacteria.

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With the rise of world fish farming, the national scenario is favorable for using native fish for intensive farming. Among the catfish, the Amazonian Jundiá (Leiarius marmoratus) is a robust candidate, easy to grow and with good organoleptic characteristics in its flesh. For productive success in c aptivity, it is necessary to consider some questions about the species, such as genetic variability, which must have an acceptable level in a breeding stoc k, in order to maintain a good diversity; this reduces losses due to inbreeding and low diversity. Therefore, the objective of this study was to characterize the genetic variability of commercial stocks of L. marmoratusfrom the State of Mato Grosso through microsatellite molecular markers. We analyzed 143 individuals from three stocks. The mean hete rozygosity and the inbreeding coefficients observed were 0.060; 0.084; 0 .141; and 0.539; 0.562; 0.514, respectively, for the stocks of Campo V erde, Juína,and Nova Mutum. The Deviation in the Hardy-Weinberg equilib rium was observed in most of the lociin the three populations. Considering the genetic differentiation, it is concluded that the three populations are very clo se genetically, which requires introduction of new genetic material in the stock s to enrich them genetically for a later reproductive program.(AU)

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RESUMEN: El objetivo de este estudio fue comparar objetivamente las diferencias en la forma total (tamaño y forma pura) del neurocráneo entre un grupo de cráneos de gatos domésticos (Felis catus, n=81), y un grupo de gato montés (Felis silvestris, n=12) mediante técnicas de morfometría geométrica. Para ello se obtuvieron fotografías digitales del lado izquierdo de los cráneos de un modo estandarizado, y en las que se ubicaron 8 landmarks y 9 semilandmarks. Después de una superposición generalizada de Procrustes se procedió a un análisis de componentes principales y a otro de canónico a fin de comparar y detectar las posibles diferencias. Los tamaños neurocraneales no mostraron diferencias entre ambos grupos, pero sí que aparecieron diferencias significativas en la forma. Los cambios de forma afectarían principalmente la sutura escamoso-parietal-alisfenoidal y, en menor medida, puntos caudales del cráneo, lo que sugeriría cambios a nivel de músculos masticatorios. A conocimiento del autor, constituye esta la primera investigación que caracteriza y compara el cráneo de los gatos mediante métodos de morfometría geométrica, recomendándose un mayor uso de esta técnica dada la escasez de publicaciones al respecto en mamíferos domésticos.

SUMMARY: The objective of this study was to objectively compare the differences in the total shape (size and pure shape) of the neurocranium between a group of domestic cat skulls (Felis catus, n=81), and wildcats (Felis silvestris, n=12) by means of geometric morphometric techniques. To achieve it, we obtained digital photographs of the left side of the skulls in a standardized form, in which 8 landmarks and 9 semilandmarks were located. After a Generalized Procrustes Superimposition, a Principal Component Analysis and a Canonical Analysis one were performed to compare and detect possible differences. Neurocranial sizes showed no differences between the two groups, however, significant differences in shape did appear. Shape changes would mainly affect the squamosal-coronal suture and, to a lesser extent, caudal skull points, therefore suggesting changes in masticatory muscles. As far as this author´s knowledge, this is the first research that characterizes and compares the skull of cats using geometric morphology methods, recommending greater use of this technique given the scarcity of publications on this subject in domestic mammals.

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Platynosomum fastosum, which is distributed in tropical and subtropical regions, is the main parasite in the biliary system of domestic cats. Cats can be asymptomatic or show severe signs of cholangitis. Although the infection is reported throughout Brazil, only post-mortem studies are available from the Midwest region of the country. This cross-sectional study investigated the frequency of P. fastosum parasitism in domestic cats treated at the University Veterinary Hospital in Cuiabá city, Mato Grosso, Brazil, by three coproparasitological techniques (formalin-ether sedimentation, Faust, and Hoffmann), associating clinical, laboratory (haematological, biochemical) and ultrasonographic alterations. Based on the sample calculation, 171 cats were evaluated in the clinical and laboratory and a semi-structured epidemiological questionnaire was designed and used by the tutors. The prevalence observed was 26.90%, with no significant association with sex, reproductive status, age, hunting habit, access to the street, recent anthelmintic treatment, and the guardian's lack of knowledge regarding parasitism. Of the 171 cats evaluated, 55 (32.16%) showed clinical signs suggestive of cholangitis, with a significant association of this variable. Regarding the clinical and pathological variables, mild neutrophilia and elevated serum activity of alanine amino transferase and alkaline phosphatase was observed (but not significantly), while the mean values of eosinophils, leucocytes, total protein, albumin, and globulin were normal. The ultrasound findings were like those previously described. Thus, it was observed that P. fastosum occurs in moderate prevalence in cats from Cuiabá-MT, with a significant association with clinical signs and a regular agreement between the parasitological techniques employed.

Assuntos

RESUMO

The menaced and poorly-known waters of the Bodoquena Plateau revealed a new resident, the stunning Hypostomus froehlichi sp. n., a large-sized armored catfish, which is finally described after more than twenty years since its discovery. The Bodoquena Plateau is drained by the rio Paraguay basin, and is located in the state of Mato Grosso do Sul, Brazil. The new species differs from its congeners on the Bodoquena crystalline waters by having teeth with morphological and numerical variation in adult specimens. There is a continuous range of specimens having about 20 thick and worn teeth to specimens having about 50 thin teeth with intact crowns and lanceolate main cusps. Additional diagnostic characters are: dentaries angled more than 90 degrees, dark blotches, one plate bordering supraoccipital, moderate keel along dorsal series of plates, usually two rows of blotches per interradial membrane on dorsal, pectoral and ventral fins, and by attaining comparatively large size. Hypostomus froehlichi seems to be endemic to the area of the Bodoquena Plateau, in rivers draining to the rio Miranda. The description of the new species reveals a potential conservation flagship species as it is one of the most seen and documented fish by visitors and divers in the clear waters from the touristic, though menaced, Bonito region in Brazil.

Assuntos