RESUMO
Introduction: Bacillus thuringiensis , has been used worldwide for the control of mosquitoes, particularly in combating Aedes aegypti. Various formulations have been made however, the effectiveness of these insecticides vary varies considerably according to the environmental conditions. Objective: to evaluate the influence of abiotic factors on the effectiveness of B. thuringiensis var. israelensis to against larvae of Ae. aegypti. Methods: we conducted bioassay experiments in the campus of the CESC / UEMA. With use of 20 Twenty cement tanks, of these, were used, 10 of which were exposed to environmental factors, and only. Of these, 5 were treated and 5 served as controls. The other 10 tanks were placed in the shade, also again 5 were treated and 5 untreated were not (control). After treatment, the 25 stage L 3 larvae were placed in 25 L 3 each stage tank and mortality. Mortality readings were taken after 24 hours and every 7 days. Weekly water samples were collected from all tanks to measure once a week to check the number of viable spores and evaluate bacterial recycling. Results and discussion: the larvicidal efficacy: The larvicide showed initial efficacy, killing 100 percent of the larvae in tanks that were located both in the sun and in the shade. Environmental factors, sunlight, temperature and rainfall negatively affected the effectiveness of the product, because for there was a reduction in the effectiveness of the product throughout the experiment. It was observed variation in the number of spores during the development of the experiment demonstrating, showing that the bacterium has potential recyclability. Conclusion: these data serve as parameters for improving to in Bti-based insecticides, in order to make the with a view to making formulations more suited toin keeping with the environmental conditions of our region(AU)
Introducción: la Bacillus thuringiensis se ha utilizado en muchas regiones del mundo para el control de los mosquitos, sobre todo en el combate contra el Aedes aegypti. Se han elaborado varias formulaciones; sin embargo, la efectividad de esos insecticidas varía considerablemente según las condiciones ambientales. Objetivo: evaluar la influencia de los factores abióticos en la efectividad de la B. thuringiensis var. israelensis contra larvas de Ae. aegypti. Métodos: se realizaron bioensayos experimentales en el campus de la CESC / UEMA. Se utilizaron 20 tanques de cemento, 10 de los cuales quedaron expuestos a factores ambientales. De ellos, 5 fueron tratados y 5 sirvieron como controles. Los otros 10 tanques fueron ubicados en la sombra, y también en este caso 5 fueron tratados y 5 no (controles). Después del tratamiento, se colocaron 25 larvas en estadio larval L3 en cada tanque. Se realizaron lecturas de mortalidad luego de 24 horas y cada 7 días. Se tomaron muestras de agua de todos los tanques una vez a la semana para comprobar el número de esporas viables y evaluar el reciclaje bacteriano. Resultados y discusión: el larvicida mostró eficacia inicial, matando el 100 por ciento de las larvas de tanques ubicados tanto al sol como en la sombra. Los factores ambientales, la luz solar, la temperatura y la lluvia afectaron negativamente la efectividad del producto, constatándose una reducción de la misma a lo largo del experimento. Se observaron variaciones en el número de esporas durante el experimento, lo que demuestra que la bacteria presenta potencial de reciclaje. Conclusión: los datos obtenidos sirven de parámetros para el mejoramiento de los insecticidas a base de Bti, con vistas a elaborar formulaciones más acordes con las condiciones ambientales de nuestra región(AU)