RESUMO
We determined the molecular epidemiology of Bordetella pertussis isolates to evaluate its potential impact on pertussis reemergence in a population of Mexico. Symptomatic and asymptomatic cases were included. Pertussis infection was confirmed by culture and real-time polymerase chain reaction (PCR). Selected B. pertussis isolates were further analysed; i.e. clonality was analysed by pulsed-field gel electrophoresis (PFGE) and ptxP-ptxA, prn, fim2 and fim3 typing was performed by PCR and sequencing. Out of 11 864 analysed samples, 687 (5.8%) were positive for pertussis, with 244 (36%) confirmed by both culture and PCR whereas 115 (17%) were positive only by culture and 328 (48%) were positive only by PCR. One predominant clone (clone A, n = 62/113; 55%) and three major subtypes (A1, A2 and A3) were identified by PFGE. All 113 selected isolates had the allelic combination ptxP3-ptxA1. The predominant clone A and the three major subtypes (A1, A2 and A3) corresponded to the emerging genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1. In conclusion, the presence of an endemic clone and three predominant subtypes belonging to the genotypes ptxP3-ptxA1-prn2-fim2-1-fim3-2 and ptxP3-ptxA1-prn2-fim2-1-fim3-1 were detected. This finding supports the global spread/expansion reported for these outbreaks associated genotypes.
Assuntos
Bordetella pertussis/classificação , Bordetella pertussis/isolamento & purificação , Genótipo , Toxina Pertussis/genética , Coqueluche/epidemiologia , Adolescente , Adulto , Idoso , Técnicas Bacteriológicas , Bordetella pertussis/genética , Criança , Pré-Escolar , Eletroforese em Gel de Campo Pulsado , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , México/epidemiologia , Pessoa de Meia-Idade , Epidemiologia Molecular , Tipagem Molecular , Reação em Cadeia da Polimerase em Tempo Real , Análise de Sequência de DNA , Coqueluche/microbiologia , Adulto JovemRESUMO
This is a report of a case of a 3-year-old boy who developed splenic abscesses after a mild plantar wound. His initial complains were fever and several distal cutaneous abscesses (septic emboli). Multiple cultures for bacteria, fungi, mycobacterium and anaerobes were performed, but all were negative. In spite of an energetic antimicrobial treatment, he continued with high fever. Echocardiography was normal. Two weeks later, a thoracic radiography showed the left hemidiafragm was elevated and a small pleural effusion. An abdominal echosonography showed multiple hypoechoic splenic lesions, and this finding was confirmed by magnetic resonance imaging. After splenectomy, the patient healed and was discharged. Anatomopathological examination of the spleen showed eight pyogenic abscesses of different size. The causal agent was not isolated.
Assuntos
Abscesso/diagnóstico , Esplenopatias/diagnóstico , Abscesso/terapia , Pré-Escolar , Terapia Combinada , Humanos , Imageamento por Ressonância Magnética , Masculino , Radiografia , Baço/diagnóstico por imagem , Baço/patologia , Esplenectomia , Esplenopatias/terapia , UltrassonografiaRESUMO
This is a case study of a six year old boy with a previous history of a contuse trauma of the cervical spinal region who later developed fever, stiffening of the neck, and signs of spinal compression. A descending myelography detected a lesion at C4. The Nuclear Magnetic Resonance study of the area showed an anterior epidural mass extending from C5 to T1 which displaced the spinal cord. Surgery revealed an epidural abscess which was drained. The patient received parenteral antibiotic therapy.
Assuntos
Abscesso/diagnóstico , Imageamento por Ressonância Magnética , Espectroscopia de Ressonância Magnética , Doenças da Medula Espinal/diagnóstico , Infecções Estafilocócicas/diagnóstico , Criança , Espaço Epidural , Humanos , MasculinoRESUMO
Four cases of trichinosis found in a family living in Iztapalapa, Mexico, D.F. are reported. The family reported to frequently eat raw sausage originally from Villanueva, Zacatecas from which Trichinella spiralis larvae were isolated. Diagnosis was difficult in the first case since no epidemiological history was associated with the patient, besides the fact that the patient showed no signs of eosinophilia and blood tests were negative. The diagnosis in this case was confirmed through muscular biopsy. The predominating symptomatology consisted in the trilogy of fever, eyelid edema and myalgias.
Assuntos
Surtos de Doenças , Triquinelose/diagnóstico , Doença Aguda , Adolescente , Animais , Criança , Pré-Escolar , Saúde da Família , Feminino , Parasitologia de Alimentos , Humanos , Carne , México/epidemiologia , Suínos , Triquinelose/complicações , Triquinelose/epidemiologiaRESUMO
We present a case of a 6 year old male who was admitted to the Hospital Infantil de México Federico Gómez with a history of previous management in a secondary care facility, during a three year period, with the diagnosis of recurrent ascites. We established the diagnosis of an omentum cystic lymphangioma and revised the international literature, emphasizing on the different of clinical presentations, study protocol, differential diagnosis and therapy.