RESUMO
In this article, I focus on Mexican immigrant women who, as care workers in various care settings in the wealthy city of Santa Barbara, California, attempt to defend aging Americans patients from devaluation and harm. To understand why vulnerable women defend more privileged citizens of the nation, I address Mexicana subjectivity. I argue that neoliberal policies have created multiple vulnerabilities for Mexican women and it is in formal care contexts where these vulnerabilities intertwine with that of their patients. Workers' feelings of shame, complicity, and empathy help explain a defense of the Other. A significant form of defense is informal sector family-based care. This article is based on ethnographic fieldwork conducted between 2009 and 2011.
Assuntos
Cuidadores/psicologia , Emigrantes e Imigrantes/psicologia , Americanos Mexicanos/psicologia , Mulheres/psicologia , Adulto , Idoso de 80 Anos ou mais , Antropologia Médica , California , Feminino , Instituição de Longa Permanência para Idosos/organização & administração , HumanosRESUMO
A C-terminal region of human endothelial actin-binding protein-280 (ABP-280 or ABP, non-muscle filamin) was subcloned and efficiently expressed in a mammalian cells system as indicated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting analysis. As predicted by the aminoacid sequence, the fragment, a 79 kD peptide (residues 1671-2361, plus 3.9 kD from an N-terminal fusion peptide included in the expression plasmid), contained the two potential cAMP-dependent protein kinase (PKA) phosphorylation sites (serine 2152 and threonine 2336) predicted to be present in this region of the molecule. Incubation of cells in the presence of cAMP-elevating agents enhanced 32P uptake into the fragment. Site-directed mutagenesis analysis indicated that serine 2152 is the unique substrate in the C-terminal region of ABP for endogenously activated PKA. The functional implications of phosphorylation of this residue, which belongs to a serine-proline motif, are discussed in terms of the role of filamin in cytoskeleton reorganization.