RESUMO
Climate change intensifies soil salinization and jeopardizes the development of crops worldwide. The accumulation of salts in plant tissue activates the defense system and triggers ethylene production thus restricting cell division. We hypothesize that the inoculation of plant growth-promoting bacteria (PGPB) producing ACC (1-aminocyclopropane-1-carboxylate) deaminase favors the development of arbuscular mycorrhizal fungi (AMF), promoting the growth of maize plants under saline stress. We investigated the efficacy of individual inoculation of PGPB, which produce ACC deaminase, as well as the co-inoculation of PGPB with Rhizophagus clarus on maize plant growth subjected to saline stress. The isolates were acquired from the bulk and rhizospheric soil of Mimosa bimucronata (DC.) Kuntze in a temporary pond located in Pernambuco State, Brazil. In the first greenhouse experiment, 10 halophilic PGPB were inoculated into maize at 0, 40 and 80â¯mM of NaCl, and in the second experiment, the PGPB that showed the best performance were co-inoculated with R. clarus in maize under the same conditions as in the first experiment. Individual PGPB inoculation benefited the number of leaves, stem diameter, root and shoot dry mass, and the photosynthetic pigments. Inoculation with PGPB 28-10 Pseudarthrobacter enclensis, 24-1â¯P. enclensis and 52â¯P. chlorophenolicus increased the chlorophyll a content by 138%, 171%, and 324% at 0, 40 and 80â¯mM NaCl, respectively, comparing to the non-inoculated control. We also highlight that the inoculation of PGPB 28-10, 28-7 Arthrobacter sp. and 52 increased the content of chlorophyll b by 72%, 98%, and 280% and carotenoids by 82%, 98%, and 290% at 0, 40 and 80â¯mM of NaCl, respectively. Co-inoculation with PGPB 28-7, 46-1 Leclercia tamurae, 70 Artrobacter sp., and 79-1 Micrococcus endophyticus significantly increased the rate of mycorrhizal colonization by roughly 50%. Furthermore, co-inoculation promoted a decrease in the accumulation of Na and K extracted from plant tissue, with an increase in salt concentration, from 40â¯mM to 80â¯mM, also favoring the establishment and development of R. clarus. In addition, co-inoculation of these PGPB with R. clarus promoted maize growth and increased plant biomass through osmoregulation and protection of the photosynthetic apparatus. The tripartite symbiosis (plant-fungus-bacterium) is likely to reprogram metabolic pathways that improve maize growth and crop yield, suggesting that the AMF-PGPB consortium can minimize damages caused by saline stress.
Assuntos
Bactérias , Carbono-Carbono Liases , Micorrizas , Raízes de Plantas , Microbiologia do Solo , Zea mays , Zea mays/microbiologia , Zea mays/crescimento & desenvolvimento , Micorrizas/fisiologia , Carbono-Carbono Liases/metabolismo , Raízes de Plantas/microbiologia , Raízes de Plantas/crescimento & desenvolvimento , Bactérias/classificação , Bactérias/metabolismo , Bactérias/isolamento & purificação , Estresse Salino , Clorofila/metabolismo , Glomeromycota/fisiologia , Tolerância ao Sal , Fotossíntese , Rizosfera , Cloreto de Sódio/metabolismo , Folhas de Planta/microbiologia , Solo/químicaRESUMO
Atriplex nummularia is a halophyte widely employed to recover saline soils and was used as a model to evaluate the water potentials in the soil-plant system under drought and salt stresses. Potted plants grown under 70 and 37% of field capacity irrigated with solutions of NaCl and of a mixture of NaCl, KCl, MgCl2 and CaCl2 reproducing six electrical conductivity (EC): 0, 5, 10, 20, 30, and 40 dS m-1. After 100 days, total water (Ψw, plant) and osmotic (Ψo, plant) potentials at predawn and midday and Ψo, soil, matric potential (Ψm, soil) and Ψw, soil were determined. The type of ion in the irrigation water did not influence the soil potential, but was altered by EC. The soil Ψo component was the largest contributor to Ψw, soil. Atriplex is surviving ECs close to 40 dS m-1 due to the decrease in the Ψw. The plants reached a Ψw of approximately -8 MPa. The water potentials determined for different moisture levels, EC levels and salt types showed huge importance for the management of this species in semiarid regions and can be used to recover salt affected soils.
Assuntos
Atriplex , Biodegradação Ambiental , Secas , Estresse Salino , Plantas Tolerantes a Sal , Solo , ÁguaRESUMO
This study aims to investigate the behavior of Atriplex nummularia under field conditions, including its growth, periodic cuttings, salt extraction, and soil chemical properties monitored for 16 months. Three treatments were evaluated: soil cultivated with Atriplex pruned at 6 and 12 months after transplanting (MAT); soil cultivated with plants that were harvested only at the end of the experiment (16 MAT); and a control (uncultivated soil) with four replications. Soil samplings were taken at 0, 6, 12, and 16 MAT. The samples were taken at depths of 0-20, 20-40, 40-60, and 60-80 cm. Biometric variables for growth were monitored monthly. The shoot was divided into leaves, thin stems (< or = 3 mm diameter), and thick stems (> 3 mm diameter) to determine its content of Ca, Mg, Na, K, and Cl. We concluded that pruning regime for Atriplex was efficient mainly because it stimulated regrowth of less lignified material (leaves and stems < or = 3 mm). We found that elements extracted by plant tissue can be quantified accurately, making them valuable indicators of the efficiency of the recovery process. The use of the Atriplex is recommended because the the possibility of revegetating areas inhospitable to most species used in conventional farming.