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1.
Microb Ecol ; 71(3): 628-33, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26500067

RESUMO

Fungi produce pigments and acids, generating particular local conditions which modify the physicochemical properties of materials. The aims of this work are (i) to investigate bioadhesion, foxing production and biofilm formation by Scopulariopsis sp. and Fusarium sp. isolated from document collections under laboratory conditions; (ii) to verify attack on cellulose fibres and (iii) to study the possibility of reducing fungal growth using natural products. Biofilm formation and extracellular polymeric substance (EPS) production by fungi were demonstrated in laboratory assays and by scanning electron microscopy (SEM) observations. The biocidal activity of two essential oils of Origanum vulgare L. and Thymus vulgaris L. was evaluated using the microatmosphere method. SEM observations showed that these strains were able to attach to paper and form biofilms, causing damage on them, which demonstrates the biodeterioration ability of these microorganisms. Scopulariopsis sp. and Fusarium sp. isolated from paper books showed the formation of fox-like reddish-brown colour spots, attack to the paper structure and pigment production on aged paper samples. The strains tested produced a decrease in the pH of one unit. This would substantiate the effect of the strains in paper biodeterioration. The microatmosphere method showed that volatile compounds of the essential oils have antifungal activity.


Assuntos
Antifúngicos/farmacologia , Fusarium/efeitos dos fármacos , Óleos Voláteis/farmacologia , Origanum/química , Óleos de Plantas/farmacologia , Scopulariopsis/efeitos dos fármacos , Thymus (Planta)/química , Biofilmes/efeitos dos fármacos , Fusarium/fisiologia , Scopulariopsis/fisiologia
4.
Colloids Surf B Biointerfaces ; 101: 337-42, 2013 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-23010038

RESUMO

Stone materials exposed to weathering are subject to biological colonization and consequently to biofilm formation, causing biodeterioration. The color changes on the stone substrates caused by biogenic pigments, mechanical stress on the mineral structure due to extracellular polymeric substances (EPS) and the accumulation of atmospheric pollutants by the biofilm are some of the deteriogenic effects, which modify esthetic and functional aspects of the work. The aim of this study was to determine biodeterioration and biofilm formation on marble tombstones from La Recoleta Cemetery, Buenos Aires, Argentina. The effect of the biocide benzalkonium chloride on biofilm formation was studied, and a chart produced of the treated tombstones. Pre- and post-intervention microbiological studies, scanning electron microscopy and X-ray dispersion analysis showed a significant decrease of the biofilm flora after treatment. However, algae of the genus Trentepohlia were difficult to eradicate. These studies are a valuable contribution to determine restoration criteria against biofilm formation, to characterize chromatic variations of biological origin on the stone and to formulate conservation and restoration policies.


Assuntos
Biodegradação Ambiental , Escultura , Microbiologia do Ar , Argentina , Bactérias/química , Compostos de Benzalcônio , Biofilmes/efeitos dos fármacos , Carbonato de Cálcio , Contagem de Colônia Microbiana , Monitoramento Ambiental , Rituais Fúnebres , Fungos/química , Microscopia Eletrônica de Varredura , Tempo (Meteorologia)
5.
Colloids Surf B Biointerfaces ; 85(2): 229-34, 2011 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-21439796

RESUMO

The aims of this paper were to study the biofouling and biodeterioration of photos and maps stored at Historical Archive of the Museum of La Plata (HAMP), Argentine, and two repositories of the National Archive of Cuba Republic (NARC) and to carry out the physiological characterization of the isolated fungi and bacteria. The role of the environmental microbiota in the biofouling formation was also studied. Microbial assemblages in the air were sampled by sedimentation technique while those on documents were sampled by swabbering. Biofilm formation and biofouling were monitored by scanning electron microscope (SEM). Large microbial assemblages were found at NARC archives with the prevalence of genera Aspergillus, Cladosporium and Penicillium, whereas at HAMP these values were lower, Penicillium was the only fungal genus detected. Most of the fungi degraded cellulose and produced pigments and acids, and all of the isolated bacteria had proteolytic and/or cellulolytic activity. In all cases, a higher concentration of viable bacteria than of fungi was isolated from documents. These results correlated with bacterial values detected in air at NARC repositories. However, this correlation cannot be observed at HAMP where Aspergillus, Penicillium and Talaromyces helicus (teleomorph of Penicillium) were isolated. It is the first time that the last genus is reported in documents.


Assuntos
Bactérias/crescimento & desenvolvimento , Incrustação Biológica , Fungos/crescimento & desenvolvimento , Museus , Microbiologia do Ar , Argentina , Aspergillus/crescimento & desenvolvimento , Aspergillus/isolamento & purificação , Aspergillus/ultraestrutura , Bacillus/crescimento & desenvolvimento , Bacillus/isolamento & purificação , Bacillus/ultraestrutura , Bactérias/isolamento & purificação , Bactérias/ultraestrutura , Cladosporium/crescimento & desenvolvimento , Cladosporium/isolamento & purificação , Cladosporium/ultraestrutura , Contagem de Colônia Microbiana , Cuba , Enterobacter/crescimento & desenvolvimento , Enterobacter/isolamento & purificação , Enterobacter/ultraestrutura , Monitoramento Ambiental/métodos , Fungos/isolamento & purificação , Fungos/ultraestrutura , Viabilidade Microbiana , Microscopia Eletrônica de Varredura , Papel , Penicillium/crescimento & desenvolvimento , Penicillium/isolamento & purificação , Penicillium/ultraestrutura , Serratia/crescimento & desenvolvimento , Serratia/isolamento & purificação , Serratia/ultraestrutura , Staphylococcus/crescimento & desenvolvimento , Staphylococcus/isolamento & purificação , Staphylococcus/ultraestrutura , Streptococcus/crescimento & desenvolvimento , Streptococcus/isolamento & purificação , Streptococcus/ultraestrutura , Streptomyces/crescimento & desenvolvimento , Streptomyces/isolamento & purificação , Streptomyces/ultraestrutura
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