RESUMO

Lunar cycle modulates the rhythmic activity patterns of many animals, including fish. The effect of the moonlight cycle on daily melatonin and metabolic parameters was evaluated in matrinxã (Brycon amazonicus) subjected to external natural lighting. Eighty juvenile were distributed in 4 tanks of 1m3 (20 fish/tank) and divided into two groups. One group was exposed to the full moon and the other group to the new moon for 30 days, which corresponds to the duration of the lunar period. At the end of the lunar phase, 6 fish from each group were anesthetized to collect blood, tissue and eye samples at midday and midnight. The comparison between the light and dark periods revealed a significant increase in plasma and ocular melatonin in the last period. However, there was no significant difference for plasma melatonin between moons. Ocular melatonin presented higher concentrations during the new moon. Glucose, total proteins, cortisol, liver glutathione and gill lipid peroxidation were higher in the full moon compared to in the new moon. Plasma triglyceride was higher during the night for the full moon, and the opposite was found for the new moon. Total cholesterol values were higher at night regardless the moon phase. Glutathione in the gills and lipid peroxidation in the liver showed no significant differences. These results highlight the importance of considering both the day and lunar cycles for melatonin and metabolic parameters in species of commercial interest and susceptible to stressful situations in rearing conditions.

Assuntos

RESUMO

Background: The hanging liver maneuver and intrahepatic extra-Glissonian approach are distinct modalities to facilitate safe anatomical liver resections. This study reports a standardized combination of these techniques focusing on safety, results and correlation with portal pedicle anatomy in oncological patients. Method: Combined hanging liver maneuver and intrahepatic extra-Glissonian approach for anatomic right hepatectomy was described stepwise. Portal pedicle anatomy was correlated with the Glissonian approach failure and complications. Clinical characteristics of patients, perioperative outcomes, short and long-term survival rates were analyzed. Results: Thirty colorectal liver metastases patients submitted to the combined approach were evaluated. Anatomical variations of the right portal pedicle were present in 26.6%. Hanging liver maneuver was feasible in 100%, and Glissonian approach in 96.7% despite portal pedicle variations. Mean operative time was 326 min. Mean blood loss was 507 ml. Mean hospital stay was 8 days. There was no 90-day operative mortality and no significant morbidity. Oncological surgical margins were free. Overall and disease-free 5-year survival were 59 and 37%. Conclusion: Regardless of frequent anatomical variations of the right portal pedicle, the hanging liver maneuver, and intrahepatic extra-Glissonian approach can be combined, being useful for anatomical right hepatectomies in a safe and reproducible way in most patients.

RESUMO

This study aimed to investigate the stress response of Sparus aurata specimens fed with nutraceutical aquafeed brewer's yeast (Saccharomyces cerevisiae) and spirulina (Arthrospira platensis). For that purpose, 96 (169.0 ± 2.8 g) animals were distributed randomly in 12 tanks (8 fish per tank, 4 replicates) and divided in 3 groups (D1, casein/gelatin, control; D2, brewer's yeast; D3, spirulina) and fed for 30 days. At the end of this period, fish from two replicates of each experimental diet were submitted to air exposure for 60 s while the fish from the other two replicates were maintained undisturbed (control). Afterwards, samples of blood, skin mucus, and head kidney were collected. The results revealed that after air exposure, cortisol, and glucose levels increased in the groups fed D1 (18.5 ± 2.6 mg/mL; 7.3 ± 0.6 mmol/L, respectively) and D2 (20.0 ± 6.2 mg/mL; 7.7 ± 0.6 mmol/L), but glucose not increased in fish fed D3 (13.7 ± 2.6 mg/mL; 5.5 ± 0.3 mmol/L). Lactate levels increased in all stressed groups, but in D1, its levels were significantly higher. After stress procedure, immunoglobulin M (IgM) levels in mucus increased only in fish fed D3 (0.1901 ± 0.0126 U/mL). Furthermore, there was a reduction in the expression of some genes involved in stress response (coxIV, prdx3, csfl-r, ucp1, and sod in fish fed D2 and D3). csf1 decreased only in stressed fish fed D2. However, cat increased in fish fed with D3. In summary, these findings points to the beneficial effects of spirulina and brewer's yeast to improve stress resistance in aquaculture practices of gilthead seabream.

Assuntos

RESUMO

The light-dark cycle and feeding can be the most important factors acting as synchronizers of biological rhythms. In this research we aimed to evaluate synchronization to feeding schedule of daily rhythms of locomotor activity and digestive enzymes of tilapia. For that purpose, 120 tilapias (65.0±0.6g) were distributed in 12 tanks (10 fish per tank) and divided into two groups. One group was fed once a day at 11:00h (zeitgeber time, ZT6) (ML group) and the other group was fed at 23:00h (ZT18) (MD group). The fish were anesthetized to collect samples of blood, stomach and midgut at 4-hour intervals over a period of 24h. Fish fed at ML showed a diurnal locomotor activity (74% of the total daily activity occurring during the light phase) and synchronization to the feeding schedule, as this group showed anticipation to the feeding time. Fish fed at MD showed a disruption in the pattern of locomotor activity and became less diurnal (59%). Alkaline protease activity in the midgut showed daily rhythm with the achrophase at the beginning of the dark phase in both ML and MD groups. Acid protease and amylase did not show significant daily rhythms. Plasma glucose showed a daily rhythm with the achrophase shifted by 12h in the ML and MD groups. These results revealed that the feeding time and light cycle synchronize differently the daily rhythms of behavior, digestive physiology and plasma metabolites in the Nile tilapia, which indicate the plasticity of the circadian system and its synchronizers.

Assuntos

RESUMO

The authors report the case of a 66-year-old male patient diagnosed with a pseudoaneurysm of the distal aorto-aortic anastomosis treated with the inner tubing technique. The patient had been operated on 1 year before when he had an aortic prosthesis implanted as treatment for a ruptured abdominal aortic aneurysm. The inner tubing technique was developed to facilitate the treatment in bifurcated vascular lesions, where endovascular conventional prosthesis is not available.

RESUMO

INTRODUCTION: Bladder exstrophy (BE) is a rare condition that requires complex surgical corrections to achieve the goals of bladder functionality, normal sexual function, continence, and finally cosmesis. The purpose of this study was to identify clinical parameters that predict better quality of life (QOL) scores using a validated questionnaire (SF-36) with young adults after completing surgical reconstruction. MATERIAL AND METHODS: Forty-three young adults (mean age 22.35 years, 29 men and 14 women) treated for BE were evaluated using the Short Form 36 general health questionnaire (SF-36). Clinical assessment involved evaluation of the actual condition regarding continence, sexual function, genital satisfaction and overall cosmesis. RESULTS: Both genders presented similar QOL scores (p = 0.36). The QOL was not age-related (p = 0.63). Neither genders did not present any differences in the number of procedures (p = 0.27). Although no significant gender difference was found, clinical impairments - such as urinary fistula, incontinence, penile length and infertility - were associated with worse QOL scores and were male-related (p <0.01). The most common complaint after complete surgical repair was about penile length (26/29 patients, 89.6%). CONCLUSIONS: Age and gender were not predictors of better QOL scores. Any clinical impairment, such as urinary leakage due to incontinence or fistula, penile length and infertility, tended to significantly decrease the overall QOL in male patients with BE. The male genitalia seems to be the most troublesome aspect post-adolescence in treated male patients with exstrophy-epispadias. It has an important impact on the overall QOL, mainly having a social affect on those patients.

RESUMO

Inflammatory bowel diseases (IBD) are characterized by chronic inflammation of the intestinal tract associated with an imbalance of the intestinal microbiota. Crohn's disease (CD) and ulcerative colitis (UC) are the most widely known types of IBD and have been the focus of attention due to their increasing incidence. Recent studies have pointed out genes associated with IBD susceptibility that, together with environment factors, may contribute to the outcome of the disease. In ulcerative colitis, there are several therapies available, depending on the stage of the disease. Aminosalicylates, corticosteroids, and cyclosporine are used to treat mild, moderate, and severe disease, respectively. In Crohn's disease, drug choices are dependent on both location and behavior of the disease. Nowadays, advances in treatments for IBD have included biological therapies, based mainly on monoclonal antibodies or fusion proteins, such as anti-TNF drugs. Notwithstanding the high cost involved, these biological therapies show a high index of remission, enabling a significant reduction in cases of surgery and hospitalization. Furthermore, migration inhibitors and new cytokine blockers are also a promising alternative for treating patients with IBD. In this review, an analysis of literature data on biological treatments for IBD is approached, with the main focus on therapies based on emerging recombinant biomolecules.

Assuntos

RESUMO

AbstractThe objective of this study was to evaluate the performance and carcass yield of finishing castrated males and females pigs, supplemented with diets containing different levels of ractopamine. Sixty animals were used, with initial body weight of 84.27 ± 2.26kg, distributed in a randomized block design, consisting of six levels of ractopamine (0; 3; 6; 9; 12 and 15ppm) and five repetitions per treatment. At the end of the trial period, it was evaluated the effect of different levels of ractopamine on the final body weight (FW), average daily feed intake (ADFI), average daily weight gain (ADG), feed conversion (FC) and carcass yield. It was observed a linear increase (P 0,05) in the levels of ractopamine for final weight, as well as for the average daily weight gain. For feed conversion (P 0,05), it was evident a decrease of 25.50% in animals supplemented with 15ppm of ractopamine in the diet. For average daily feed intake and carcass yield there was no significant effect (P>0,05). The supplementation with ractopamine in the diet of finishing castrated males and females pigs, provides greater performance to the animals, improving production yield.

ResumoO objetivo com este trabalho foi avaliar o desempenho e o rendimento de carcaça de suínos na fase de terminação de machos castrados e fêmeas, recebendo dietas contendo diferentes níveis de ractopamina. Foram utilizados 60 animais, com peso inicial de 84,27 ± 2,26kg, aleatoriamente distribuídos para uma das seis dietas experimentais compostas por diferentes níveis de ractopamina (zero; três; seis; nove; 12 e 15ppm) e cinco repetições por tratamento. Ao final do período experimental, foi avaliado o efeito dos diferentes níveis de ractopamina sobre o peso final (PF), consumo de ração médio diário (CRMD), ganho de peso médio diário (GPMD), conversão alimentar (CA) e rendimento de carcaça. Observou-se aumento linear (P 0,05) para peso final e ganho de peso médio diário, à medida que houve aumento dos níveis de ractopamina. Para a conversão alimentar (P 0,05), evidenciou-se decréscimo de 25,50% nos animais que receberam 15ppm de ractopamina na dieta. Para o consumo de ração médio diário e rendimento de carcaça, não foram observados efeitos significativos (P>0,05). A adição de ractopamina na dieta de suínos machos castrados e fêmeas em terminação proporcionou melhor desempenho aos animais, promovendo melhoras nos parâmetros produtivos.

RESUMO

AbstractThe objective of this study was to evaluate the performance and carcass yield of finishing castrated males and females pigs, supplemented with diets containing different levels of ractopamine. Sixty animals were used, with initial body weight of 84.27 ± 2.26kg, distributed in a randomized block design, consisting of six levels of ractopamine (0; 3; 6; 9; 12 and 15ppm) and five repetitions per treatment. At the end of the trial period, it was evaluated the effect of different levels of ractopamine on the final body weight (FW), average daily feed intake (ADFI), average daily weight gain (ADG), feed conversion (FC) and carcass yield. It was observed a linear increase (P 0,05) in the levels of ractopamine for final weight, as well as for the average daily weight gain. For feed conversion (P 0,05), it was evident a decrease of 25.50% in animals supplemented with 15ppm of ractopamine in the diet. For average daily feed intake and carcass yield there was no significant effect (P>0,05). The supplementation with ractopamine in the diet of finishing castrated males and females pigs, provides greater performance to the animals, improving production yield.

ResumoO objetivo com este trabalho foi avaliar o desempenho e o rendimento de carcaça de suínos na fase de terminação de machos castrados e fêmeas, recebendo dietas contendo diferentes níveis de ractopamina. Foram utilizados 60 animais, com peso inicial de 84,27 ± 2,26kg, aleatoriamente distribuídos para uma das seis dietas experimentais compostas por diferentes níveis de ractopamina (zero; três; seis; nove; 12 e 15ppm) e cinco repetições por tratamento. Ao final do período experimental, foi avaliado o efeito dos diferentes níveis de ractopamina sobre o peso final (PF), consumo de ração médio diário (CRMD), ganho de peso médio diário (GPMD), conversão alimentar (CA) e rendimento de carcaça. Observou-se aumento linear (P 0,05) para peso final e ganho de peso médio diário, à medida que houve aumento dos níveis de ractopamina. Para a conversão alimentar (P 0,05), evidenciou-se decréscimo de 25,50% nos animais que receberam 15ppm de ractopamina na dieta. Para o consumo de ração médio diário e rendimento de carcaça, não foram observados efeitos significativos (P>0,05). A adição de ractopamina na dieta de suínos machos castrados e fêmeas em terminação proporcionou melhor desempenho aos animais, promovendo melhoras nos parâmetros produtivos.

RESUMO

The present work aimed to evaluate the effect of metionine dietary levels on performance and chemical composition from Nile tilapia, during sex reversal phase. A 35 liter-aquarium with 15 animals was considered as an experimental unit. The treatments had been constituted by larvae feeding with diets containing 0.56; 0.65; 0.75; 0.85; 0.95% of total metionine. The animals were fed on ad libitum regimen. In the end of the experimental period, the performance parameters of length and weight, daily weigh gain, factor condition and survival were evaluated. The carcass chemical characteristics from animals as moisture, mineral matter, crude protein and eter extract were also evaluated. The data were submitted to the variance analysis (=0,05). No difference (P >0.05) among treatments was observed. Results show that in rations for Nile tilapia, in this phase, the use of synthetic metionine affected neither the performance nor chemical composition of the animals

O trabalho teve objetivo de avaliar o efeito de diferentes níveis dietéticos de metionina na nutrição da tilápia do Nilo, durante a fase de reversão sexual. Foi considerada como uma unidade experimental um aquário de 35 litros, contendo 15 larvas. Os tratamentos foram constituídos pela alimentação das larvas com dietas contendo 0,56%, 0,65%, 0,75%, 0,85%, 0,95% de metionina total. Os animais foram alimentados ad libitum. No final do experimento, foram avaliados os parâmetros de peso e comprimento médio, ganho de peso diário, fator de condição, sobrevivência e as características bromatológicas da carcaça dos animais de umidade, matéria mineral, proteína bruta e extrato etéreo. Os dados foram submetidos à análise de variância (=0,05). Ao final do experimento, não foi observada diferença (p >0,05) entre os tratamentos. Conclui-se que em rações para tilápia do Nilo, na fase estudada, a suplementação de metionina sintética não influenciou no desempenho nem na composição corporal dos animais

RESUMO

The present work aimed to evaluate the effect of metionine dietary levels on performance and chemical composition from Nile tilapia, during sex reversal phase. A 35 liter-aquarium with 15 animals was considered as an experimental unit. The treatments had been constituted by larvae feeding with diets containing 0.56; 0.65; 0.75; 0.85; 0.95% of total metionine. The animals were fed on ad libitum regimen. In the end of the experimental period, the performance parameters of length and weight, daily weigh gain, factor condition and survival were evaluated. The carcass chemical characteristics from animals as moisture, mineral matter, crude protein and eter extract were also evaluated. The data were submitted to the variance analysis (=0,05). No difference (P >0.05) among treatments was observed. Results show that in rations for Nile tilapia, in this phase, the use of synthetic metionine affected neither the performance nor chemical composition of the animals

O trabalho teve objetivo de avaliar o efeito de diferentes níveis dietéticos de metionina na nutrição da tilápia do Nilo, durante a fase de reversão sexual. Foi considerada como uma unidade experimental um aquário de 35 litros, contendo 15 larvas. Os tratamentos foram constituídos pela alimentação das larvas com dietas contendo 0,56%, 0,65%, 0,75%, 0,85%, 0,95% de metionina total. Os animais foram alimentados ad libitum. No final do experimento, foram avaliados os parâmetros de peso e comprimento médio, ganho de peso diário, fator de condição, sobrevivência e as características bromatológicas da carcaça dos animais de umidade, matéria mineral, proteína bruta e extrato etéreo. Os dados foram submetidos à análise de variância (=0,05). Ao final do experimento, não foi observada diferença (p >0,05) entre os tratamentos. Conclui-se que em rações para tilápia do Nilo, na fase estudada, a suplementação de metionina sintética não influenciou no desempenho nem na composição corporal dos animais