RESUMO

This study aimed to investigate the occurrence and the genetic diversity of Salmonella enterica subsp. enterica in sausages from Southern Brazil, evaluate virulence genes and determine the phenotypic and genotypic basis of antimicrobial and sanitizer resistance. Salmonella was detected in sausage samples with an overall prevalence of 5.5%. The prevalent serovars were S. Infantis and S. Rissen. Pulsed-field gel electrophoresis (PFGE) analysis yielded nine distinct PFGE profiles, and some of them were recurrently recovered in the same establishment on different dates. Among tested isolates, 28.5% showed resistance to at least one antimicrobial agent and a multidrug-resistance (MDR) profile was observed in 21.4%. Resistance occurred most frequently to ampicillin, sulfonamide, trimethoprim/sulfamethoxazole, and trimethoprim. Regarding the genotypic antimicrobial resistance profile, S. Schwarzengrund carried tet(B), strA, strB, and sul2 genes. Benzalkonium chloride and chlorhexidine were more effective than peracetic acid and sodium hypochlorite, showing lower minimum inhibitory concentration values. Six Salmonella serovars were found, demonstrating a potential risk of salmonellosis associated with consuming this food. Salmonella carrying virulence genes, MDR profile, and tolerance to sanitizers is a public health concern and a challenge for the food industry, suggesting that new strategies should be developed to control this pathogen. Supplementary Information: The online version contains supplementary material available at 10.1007/s13197-023-05809-w.

RESUMO

Salmonellosis, caused by the consumption of contaminated foods, is a major health problem worldwide. The aims of this study were to assess the susceptibility of Salmonella spp. isolates to benzalkonium chloride (BC) disinfectant and the antimicrobial activity of Butia odorata Barb. Rodr. extract against the same isolates from food and food environments. Moreover, phenotypic and genotypic resistance profiles, the presence of virulence genes and biofilm forming ability were determined. The minimum inhibitory concentration (MIC) of B. odorata extract against Salmonella spp. ranged from 10 to >19 mg.mL-1. Resistance to ampicillin, streptomycin, nalidixic acid, sulfonamide, trimethoprim/sulfamethoxazole, trimethoprim, tetracycline, and chloramphenicol was observed. In addition, multidrug resistance was observed in seven isolates (26.92%). The MIC of BC ranged from 32 to 64 mg.L-1, higher concentrations in comparison with wild-type MICs, and therefore were considered tolerant. Several resistance genes were detected, of which the most common were aadA, qacEΔ1, blaTEM, int1, sul1, and tetA. All isolates carried at least one virulence gene and produced biofilms on stainless steel surfaces at 10 and 22 °C. On the other hand, the B. odorata extract showed activity against Salmonella spp., and it has the potential to be used as a natural antimicrobial to control this important foodborne pathogen, despite its virulence potential and antimicrobial resistance profile.

Assuntos

Antibacterianos/farmacologia , Arecaceae/química , Compostos de Benzalcônio/farmacologia , Biofilmes/efeitos dos fármacos , Farmacorresistência Bacteriana Múltipla , Extratos Vegetais/farmacologia , Intoxicação Alimentar por Salmonella/prevenção & controle , Salmonella/efeitos dos fármacos , Antibacterianos/isolamento & purificação , Biofilmes/crescimento & desenvolvimento , Farmacorresistência Bacteriana Múltipla/genética , Microbiologia de Alimentos , Testes de Sensibilidade Microbiana , Extratos Vegetais/isolamento & purificação , Salmonella/genética , Salmonella/crescimento & desenvolvimento , Salmonella/patogenicidade , Intoxicação Alimentar por Salmonella/microbiologia , Virulência

RESUMO

The objective of this study was to evaluate the efficacy of a product based on n-alkyl dimethyl benzyl ammonium chloride-40%, marketed as Timsen®, during scalding and precooling of poultry carcasses in slaughterhouses. To this end, three poultry slaughterhouses (A, B and C) were evaluated. The product was added (200 ppm) to the scalding (58 °C) and precooling water (4 °C), and microbiological analyses were performed of the water and the poultry carcasses before and after Timsen® addition. The product controlled the multiplication of aerobic mesophilic microorganisms, both in the scalding as in the precooling water. In a comparison of carcasses soaked in Timsen®-treated scalding and precooling water with carcasses soaked in untreated water, the count of aerobic mesophilic microorganisms in the later was higher and thermotolerant coliform was not detected in samples of carcasses soaked in Timsen®-treated water. When the scalding and precooling water was not treated with the product, Listeria spp. was isolated from poultry carcasses of two slaughterhouses (A and C), while these microorganisms were not detected when Timsen® was applied. The use of Timsen® in the scalding and precooling water enhanced the safety and control microbial contamination of poultry carcasses.

RESUMO

Listeria monocytogenes is a bacterium capable to adhere to the surfaces of equipment and utensils and subsequently form biofilms. It can to persist in the food processing environmental for extended periods of time being able to contaminate the final product. The aim of this study was to trace the contamination route of L. monocytogenes on a fresh mixed sausage processing line, from raw material to the final product. The isolates obtained were characterized by serotyping and molecular typing by pulsed-field gel electrophoresis (PFGE) using the restriction enzymes ApaI and AscI. L. monocytogenes was detected in 25% of the samples. The samples of raw material were not contaminated, however, the microorganism was detected in 21% of the environmental samples (food contact and non-food contact), 20.8% of the equipments, 20% of the food worker's hands, 40% of the mass ready to packaging and in all the final products samples, demonstrating that the contamination of final product occurred during the processing and the importance of cross contamination. PFGE yielded 22 pulsotypes wich formed 7 clusters, and serotyping yielded 3 serotypes and 1 serogroup, however, the presence of serotypes 4b and 1/2b in the final product is of great concern for public health. The tracing of contamination showed that some strains are adapted and persisted in the processing environment in this industry.