RESUMO
Obesity is characterized by immune cell infiltration and inflammation. Purinergic receptors such as P2X1, 4 and 7 are expressed on immune cells and their activation contributes with an inflammatory response. However, the simultaneous expression of P2X1, 4 and 7 during overweight or obesity have not been described. Therefore, the aim of this study was to determine single and simultaneously expression and function of the P2X1, 4 and 7 receptors in lymphocytes and CD4â¯+â¯T cells from peripheral blood (PB) and adipose tissue (AT). Our results showed a higher expression of the P2X4 receptor on CD4â¯+â¯T cells from PB regarding P2X7 and P2X1 receptor expression. In addition, P2X4 receptor expression on CD4â¯+â¯T cells from PB and AT was increased in individuals with BMIâ¯≥â¯25 Kg/m2. Moreover, a higher simultaneous expression of the P2X4 and P2X7 receptors on CD4â¯+â¯T cells from AT compared to CD4â¯+â¯T cells expressing P2X1 and P2X7 receptors simultaneously. Besides, CD4â¯+â¯T cells expressing P2X4 and P2X7 receptors from PB and AT were augmented in individuals with BMIâ¯≥â¯25 Kg/m2. In addition, the percentage of lymphocytes and also CD4â¯+â¯T cells expressing P2X4 receptor were elevated both in PB and AT compared to cells expressing P2X7 or P2X1. However, CD4â¯+â¯T cells expressing P2X4 and P2X7 were augmented in AT compared to PB. The function of the receptors showed a lower shedding of CD62â¯L in adipose tissue mononuclear cells (ATMC) compared with peripheral blood mononuclear cells (PBMC) and a greater participation of P2X4 in the mobilization of intracellular calcium. We concluded that it was possible to determine for the first time the simultaneous expression of purinergic receptors in ATMC, where the P2X4 receptor has a greater participation in the activation of CD4â¯+â¯T cells possibly modulating the function of the other two receptors.