RESUMO
Interrelationships between Erythrocyte Protoporphyrin (EP), dietary Iron/Protein ratio (Fe/Prot) and Fe liver content (Feh) were studied during nutritional recovery in an experimental model: weanling female Wistar rats (To) were depleted with a protein-free diet (LP), losing 20% of their initial body weight. Then they were recovered until 45 days of age (T45) with diets containing: casein: 20 g/100 g; Fe (ammonium Fe citrate) (ppm.): 0, 75 or 100 (groups A1, A2 and A3, respectively). Hematocrit, Hemoglobin (Hb) (g/dL). Erythrocyte Protoporphyrin (EP) (microgram/dL Red Blood Cells) and Feh (microgram) were determined at To, LP and T45. Results were compared with control rats (C) fed with 20% of casein and Fe, 50 ppm. EP: a) decreased in C from To to T45 (99 +/- 24; 36 +/- 9; p < 0.01); b) increased in A1 and A2 at T45 (123 +/- 21; 93 +/- 29, respectively, p < 0.01) while A3 did not show significant difference (45 +/- 7) regarding to C: c) correlated inversely with Feh. According to the inverse correlation between EP and Fe/Prot (r = -0.99), we found that 92 ppm was an adequate Fe amount to prevent EP increase. These results confirm that during recovery from undernutrition EP depends on iron liver content, being an adequate indicator of iron nutritional status; therefore, EP would be useful as a predictor of the optimum Fe/Prot ratio for nutritional recovery.
Assuntos
Eritrócitos/química , Ferro da Dieta/sangue , Distúrbios Nutricionais/sangue , Estado Nutricional , Protoporfirinas/sangue , Fatores Etários , Animais , Peso Corporal , Feminino , Hematócrito , Hemoglobina A/química , Avaliação Nutricional , Distúrbios Nutricionais/dietoterapia , Ratos , Ratos WistarRESUMO
We have previously reported that, in the first stages of nutritional recovery, the needs of undernourished infants, in terms of protein-calories percentage (P%) are higher than normal, producing an accelerated catch-up. Consequently, calcium (Ca) and phosphorus (P) balances increase proportionally to weight gain rate (WGR) in order to attain a normal body composition. As for the control of Ca metabolism by vitamin D, the question is whether vitamin D intake must also be increased during this accelerated catch-up growth period. In this report we have analysed retrospectively previous results in order to clarify the interrelationship between Ca absorption, Ca retention and vitamin D intake. A total of 29 undernourished infants, from 2 to 18 months of age, were fed Ad libitum with one of two proprietary milk formulas containing, per 100 g.: Ca, 0.50 and 1.17 g.; P, 0.37 and 0.50 g.; vitamin D, 350 and 400 UI (8.7 and 10 micrograms); proteins, 11.34 and 17.00 kcal/100 kcal of total diet, respectively. Recommended Dietary Allowances (RDA) of Ca and vitamin D, according to FAO/OMS and NRC, were calculated with reference to normal infants body weight and to 1000 kcal of recommended dietary energy intake. Ca intake was higher than the RDA and ranged between 60 and 413 mg/kg/day; intake of vitamin D increased proportionally to food intake; calcium absorption ranged between 40.0 and 92.0% (mean = 69.8 +/- 14.6) and CaB correlated with Ca intake (r = 0.97), being higher than normal.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Cálcio/administração & dosagem , Alimentos Infantis , Necessidades Nutricionais , Desnutrição Proteico-Calórica/terapia , Vitamina D/administração & dosagem , Cálcio/metabolismo , Humanos , Lactente , Masculino , Estudos Retrospectivos , Vitamina D/metabolismo , Aumento de Peso/efeitos dos fármacosRESUMO
We have previously reported that, in the first stages of nutritional recovery, the needs of undernourished infants, in terms of protein-calories percentage (P
) are higher than normal, producing an accelerated catch-up. Consequently, calcium (Ca) and phosphorus (P) balances increase proportionally to weight gain rate (WGR) in order to attain a normal body composition. As for the control of Ca metabolism by vitamin D, the question is whether vitamin D intake must also be increased during this accelerated catch-up growth period. In this report we have analysed retrospectively previous results in order to clarify the interrelationship between Ca absorption, Ca retention and vitamin D intake. A total of 29 undernourished infants, from 2 to 18 months of age, were fed Ad libitum with one of two proprietary milk formulas containing, per 100 g.: Ca, 0.50 and 1.17 g.; P, 0.37 and 0.50 g.; vitamin D, 350 and 400 UI (8.7 and 10 micrograms); proteins, 11.34 and 17.00 kcal/100 kcal of total diet, respectively. Recommended Dietary Allowances (RDA) of Ca and vitamin D, according to FAO/OMS and NRC, were calculated with reference to normal infants body weight and to 1000 kcal of recommended dietary energy intake. Ca intake was higher than the RDA and ranged between 60 and 413 mg/kg/day; intake of vitamin D increased proportionally to food intake; calcium absorption ranged between 40.0 and 92.0
(mean = 69.8 +/- 14.6) and CaB correlated with Ca intake (r = 0.97), being higher than normal.(ABSTRACT TRUNCATED AT 250 WORDS)