RESUMO
Cytomegalovirus infection is the first cause of viral congenital infections. We studied the incidence of primary cytomegalovirus infection, searching for the presence of antibodies with an ELISA technique, in 939 pregnant women of a low socioeconomic level, attending a public outpatient clinic and 123 pregnant university students, attending a special outpatient clinic for students. The initially seronegative women were tested again during the second and third trimester of pregnancy to identify primary infections. The presence of cytomegalovirus congenital infection in the newborns of infected mothers was investigated isolating the virus in cell cultures from urine samples. There was a higher prevalence of infection among low socioeconomic status women (95 vs 69.9%). Two women (one student and one coming from a low socioeconomic status) had a primary infection and in the newborn of the student, a congenital cytomegalovirus infection was detected. It is concluded that women's socioeconomic condition is not a risk factor for cytomegalovirus primary infection during pregnancy.
Assuntos
Infecções por Citomegalovirus/epidemiologia , Complicações Infecciosas na Gravidez/epidemiologia , Adulto , Feminino , Humanos , Incidência , Recém-Nascido , Transmissão Vertical de Doenças Infecciosas , Gravidez , Prevalência , Estudos Prospectivos , Estudos Soroepidemiológicos , Fatores SocioeconômicosRESUMO
Virus isolation tests were performed during the first, second, and third trimesters of pregnancy on a group of 163 students who received prenatal care at the University of Chile's Student Medical and Dental Service to determine the possible existence of genital herpes or cytomegalovirus infections. Vaginal secretion samples tested for herpes simplex virus (HSV) and urine samples tested for cytomegalovirus (CMV) were inoculated onto cell cultures and examined by immunofluorescent procedures using monoclonal antibodies. HSV was detected in five subjects, only one of whom had herpetic lesions, and was identified as type 2 in all cases. Cytomegalovirus was detected in seven subjects.
Assuntos
Infecções por Citomegalovirus/epidemiologia , Herpes Genital/epidemiologia , Complicações Infecciosas na Gravidez/epidemiologia , Doenças Vaginais/epidemiologia , Adulto , Chile/epidemiologia , Infecções por Citomegalovirus/diagnóstico , Feminino , Herpes Genital/diagnóstico , Humanos , Gravidez , Complicações Infecciosas na Gravidez/diagnóstico , Serviços de Saúde para Estudantes , Estudantes/estatística & dados numéricos , Universidades , Doenças Vaginais/diagnósticoRESUMO
Virus isolation tests were performed on a group of 163 students who received prenatal care at the Student Medical and Dental Service at the University of Chile (Santiago) during the 1st, 2nd, and 3rd trimesters of pregnancy to determine the possible existence of genital herpes infection (isolation from vaginal secretions) or cytomegalovirus infection (isolation from urine). The samples were inoculated on cell cultures and were then studied using immunofluorescence with monoclonal antibodies. The herpes simplex virus (HSV) was detected in the vaginal secretions of 5 pregnant women, only one of whom had herpes lesions; in all these cases, the virus isolated was HSV type 2. Cytomegalovirus was detected in 7 of the pregnant women.
Assuntos
Infecções por Citomegalovirus/epidemiologia , Herpes Simples/epidemiologia , Complicações Infecciosas na Gravidez/epidemiologia , Estudantes , Chile/epidemiologia , Citomegalovirus/isolamento & purificação , Infecções por Citomegalovirus/microbiologia , Infecções por Citomegalovirus/prevenção & controle , Feminino , Herpes Simples/microbiologia , Herpes Simples/prevenção & controle , Humanos , Recém-Nascido , Doenças do Recém-Nascido/prevenção & controle , Programas de Rastreamento , Gravidez , Complicações Infecciosas na Gravidez/microbiologia , Prevalência , Simplexvirus/isolamento & purificação , Universidades , Urina/microbiologia , Vagina/microbiologia , Cultura de VírusRESUMO
We analyzed 33 patients with herpes genitalis diagnosed at a student medical outpatient facility. 42% corresponded to a first herpetic infection. The HSV-1 was present in 43% of cases, the HSV-2 in the rest. Recurrence of infection was present in 39.5% of patients, 70% of them due to HSV-2. There was a relatively high rate of asymptomatic couples who were the source of primary herpetic infection.
Assuntos
Herpes Genital/epidemiologia , Estudantes , Adulto , Feminino , Herpes Genital/microbiologia , Herpes Genital/transmissão , Humanos , Recidiva , Estudos Retrospectivos , Comportamento Sexual , Parceiros Sexuais , Simplexvirus/isolamento & purificaçãoRESUMO
This article reports on the prevalence of genital herpes infection in a sample of 635 female university students who were seen at the Gynecology Center of the University of Chile Student Medical Service. Viral isolation of genital secretions yielded 18 students (2.8%) with positive results, of whom 28% were asymptomatic shedders. Of the positive cases, 61% were clinically classified as recurrent and 39% as first episodes of genital herpes. Fluorescent monoclonal antibody typing of the isolated strains revealed that 72% were herpes simplex type 2 and 28% were type 1. The results confirm the importance of type 2 virus in genital herpes infections. They also point to asymptomatic shedders as a potential source of infection, and show the importance of a history of genital herpes in the identification of this population.