RESUMO

Rats were injected with rat recombinant (rr) IL3, rrSCF, rrIL-3+rrSCF, rrRANTES and LTB4. Six hours after subcutaneous injection of rrIL-3 or rrIL-3+rrSCF, there was an increase in mast cell numbers in the skin and spleen. Peritoneal mast cells were recruited following i.p. injection of rrIL-3, but with rrIL-3+rrSCF recruitment was delayed. Immunostaining with a mast cell specific antibody showed that immature orthochromatic mast cells were being recruited. rrIL-3 induced recruitment of mast cells to the peritoneal cavity was blocked by anti-integrin antibodies. Mast cell recruitment depended on the target tissue and the time of exposure to the chemoattractant.

Assuntos

Fatores Quimiotáticos/imunologia , Mastócitos/imunologia , Animais , Movimento Celular/imunologia , Quimiocina CCL5/farmacologia , Fatores Quimiotáticos/farmacologia , Interleucina-3/farmacologia , Leucotrieno B4/farmacologia , Masculino , Mastócitos/efeitos dos fármacos , Cavidade Peritoneal , Ratos , Ratos Wistar , Proteínas Recombinantes/farmacologia , Pele/citologia , Pele/imunologia , Baço/citologia , Baço/imunologia , Fator de Células-Tronco/farmacologia