RESUMO
This work reports on the results of double immunodiffusion (ID), counterimmunoelectrophoresis (CIE), complement fixation (CF) and indirect immunofluorescence (IIF) techniques in the serodiagnosis of paracoccidioidomycosis. The study was undertaken on four groups of individuals: 46 patients with untreated paracoccidioidomycosis, 22 patients with other deep mycoses, 30 with other infectious diseases (tuberculosis and cutaneous leishmaniasis) and 47 blood donors as negative controls. Data were obtained using Paracoccidioides brasiliensis antigens, i.e., a yeast culture filtrate for ID, CIE and CF, and a yeast cell suspension for IIF. The sensitivity, specificity and efficiency values were measured according to GALEN & GAMBINO. The gel precipitation tests (ID and CIE) showed the greatest sensitivity (91.3 and 95.6%, respectively), maximum specificity (100%) and the highest efficiency values when compared to the CF and IIF tests.
Assuntos
Paracoccidioidomicose/diagnóstico , Testes de Fixação de Complemento , Contraimunoeletroforese , Feminino , Imunofluorescência , Humanos , Imunodifusão , Masculino , Valor Preditivo dos TestesRESUMO
Fifteen Paracoccidioides brasiliensis strains, in the mycelial (M) and yeast like (Y), were cultivated in minimal medium (MM) and subcultivated to be adapted to the same medium supplemented with a pool of amino acid in solution (MMS). Each of the amino acids were studied separately of the solution to provide the auxological study. The prototrophism was demonstrated by nine strains in both M and Y forms, and the auxotrophism by the remaining strains. The heterologous results has not allowed us to draw an auxological characterization of the P. brasiliensis. As far as we could observe none of the amino acid studied in this piece of research can be considered of absolute importance for to the growth and the morphogenesis maintainance of the fungus. Morphological alterations were only verified in the prototroph strains, which suggest that there could have been adaptative metabolism activity due to the absence of organic nitrogen compounds in the minimal medium (MM).