RESUMO
OBJECTIVE: The purpose of this study was to fine map the locus Xq25.1-27-2 in order to identify genetic contributors involved in low caries experience. DESIGN: Seventy-two families from the Philippines were studied. Caries experience was recorded and genomic DNA extracted from peripheral blood was obtained from all subjects. One hundred and twenty-eight polymorphisms in the locus Xq25.1-27-2, a region that contains 24 genes, were genotyped. Association between caries experience and alleles was tested using the transmission disequilibrium test (TDT). This initial analysis was followed by experiments with DNA samples from 1481 subjects from Pittsburgh, 918 children from Brazil, and 275 children from Turkey in order to follow up the results found in the Filipino families. Chi-square or Fisher's exact tests were used. Sequencing of the coding regions and exon-intron boundaries of MST4 and FGF13 were also performed on 91 women from Pittsburgh. RESULTS: Statistically significant association with low caries experience was found for 11 markers in Xq25.1-27-2 in the Filipino families. One marker was in MST4, another marker was in FGF13, and the remaining markers were in intergenic regions. Haplotype analysis also confirmed these results, but the follow up studies with DNA samples from Pittsburgh, Brazil, and Turkey showed associations for a subset of the 11 markers. No coding mutations were identified by sequencing. CONCLUSIONS: Our study failed to conclusively demonstrate that genetic factors in Xq25.1-27-2 contribute to caries experience in multiple populations.
Assuntos
Cromossomos Humanos X/genética , Cárie Dentária/genética , Adolescente , Adulto , Idoso , Alelos , Brasil/epidemiologia , Criança , Pré-Escolar , Mapeamento Cromossômico , Cárie Dentária/epidemiologia , Éxons , Feminino , Marcadores Genéticos , Predisposição Genética para Doença , Genótipo , Haplótipos , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Pennsylvania/epidemiologia , Fenótipo , Filipinas/epidemiologia , Reação em Cadeia da Polimerase , Polimorfismo de Nucleotídeo Único , Fatores Sexuais , Turquia/epidemiologiaRESUMO
The purpose of this study was to assess the effectiveness of three oral health indicators in monitoring a Program, compare them with each other and select the most indicated in epidemiological surveys. A dental plaque index, interdental bleeding index, and saliva test were used to evaluate 325 children (6.1 to 11.3 years-old). The results indicated a decrease in all indexes (p < 0.001). The indicators were effective although the saliva test was simpler and faster than the other indexes.
Assuntos
Promoção da Saúde , Saúde Bucal , Brasil/epidemiologia , Distribuição de Qui-Quadrado , Criança , Índice de Placa Dentária , Feminino , Humanos , Masculino , Índice Periodontal , Avaliação de Programas e Projetos de Saúde , Saliva/microbiologia , Estatísticas não ParamétricasRESUMO
The purpose of this study was to implement an Oral Health Promotion Program (OHPP) and check its effectiveness using different indicators at two different times (birth to 10 months after the OHPP). The sample consisted of 325 healthy children (6.1 and 11.3 years old). The results indicated that there was a statistically significant reduction measured by the three indicators (p<0.001). The effectiveness of the OHPP was positive and clearly shown by the oral health indicators used.