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The present study evaluated the general welfare state of two strains of transgenic goats bred in a region with a hot and humid tropical climate. Nine females were used, being three transgenic for human lysozyme (hLZ group), three transgenic for human glucocerebrosidase (hGCase group), and three non-transgenic (control group). The temperature and humidity index (THI) were recorded during the morning, afternoon, and evening. The physiological parameters measured were respiratory rate, heart rate, and rectal and vaginal temperatures. Venous blood samples were collected using Vacutainer® tubes containing 10% ethylenediaminetetraacetic acid (EDTA). Also, analysis of erythrogram, leukogram, and some biochemical parameters of serum was performed. It was observed that the afternoon shift presented the largest THI, being potentially more impactful on the physiology of animals. In general, respiratory and heart rates were higher in transgenic animals, especially in the hLZ group compared to the control group (P < 0.05). Regarding the hematological parameters, the quantification of red blood cells, hemoglobin, and hematocrit was significantly lower (P < 0.05) in the hGCase group compared to that in the hLZ and control. The leukocyte count was considerably lower (P < 0.05) in the hLZ group compared to that in the hGCase and control. Correlation analysis showed that the increase in THI was associated with a change in physiological parameters normally used as indicators of thermal stress. Despite the differences found among the experimental groups, all the physiological parameters remained within the normal limits recommended for the goat species. Further studies involving a larger number of animals from different categories should be carried out to elucidate the impacts that transgenesis can have on animal welfare under different THI conditions.
Assuntos
Cabras , Clima Tropical , Animais , Animais Geneticamente Modificados , Feminino , Cabras/genética , Temperatura Alta , Umidade , TemperaturaRESUMO
Public health institutions with sectorized structure and low integration among field teams, old-fashioned practices such as paper-based storage system, and poorly qualified health agents have limited ability to conduct accurate surveillance and design effective timely interventions. Herein, we describe the steps taken by the Zoonosis Control Center of Foz do Iguaçu (CCZ-Foz) in the last 23 years to move from an archaic and sectorized structure to a modern and timely surveillance program embracing zoonotic diseases, venomous animal injuries, and vector-borne diseases epidemiology under the One Health approach. The full implementation of the One Health approach was based on 5 axes: (1) merging sectorized field teams; (2) adoption of digital solutions; (3) health agents empowerment and permanent capacitation; (4) social mobilization; and (5) active surveys. By doing so, notifications related to zoonotic diseases and venomous animals increased 10 and 21 times, respectively, with no impairment on arbovirus surveillance (major concern in the city). Open sources database (PostgreSQL) and software (QGis) are daily updated and create real-time maps to support timely decisions. The adoption of One Health approach increased preparedness for endemic diseases and reemerging and emerging threats such as Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2).
Assuntos
Mordeduras e Picadas/epidemiologia , Monitoramento Epidemiológico , Saúde Única , Doenças Transmitidas por Vetores/epidemiologia , Zoonoses/epidemiologia , Animais , Brasil , Bases de Dados Factuais , Sistemas de Informação Geográfica , Humanos , Organizações/organização & administraçãoRESUMO
The aim of this study was to evaluate the phytochemical profile of Cinnamomum zeylanicum essential oil (CZEO) and their antimicrobial and antibiofilm activity against Staphylococcus strains isolated from canine otitis. First, the CZEO chemical composition was determined by gas chromatography-mass spectrometry (CG-MS). External otitis samples collected from dogs were submitted to staphylococcal isolation, followed by MALDI-TOF mass spectrometry identification. The antimicrobial action was tested against the isolates using the disk-diffusion and microdilution methods. The antibiofilm activity was evaluated by CZEO-based concentrations, subMIC for biofilm formation and supraMIC against preformed biofilm, quantified by crystal violet (CV) staining and CFU counting. The chemical analysis revealed that (E)-cinnamaldehyde, eugenol and (E)-cinnamyl acetate were the main compounds in the CZEO, representing 77.42, 8.17 and 4.50%, respectively. Two strains of three different species, S. saprophyticus, S. schleiferi and S. pseudintermedius, were identified. The disk-diffusion test showed an inhibitory zone diameter, ranging from 34.0 to 49.5 mm, while the MIC and MBC values were around 500 and 1000 µg/mL. SubMIC demonstrated an inhibition on biofilm formation against 4 out the 6 strains tested. On mature biofilm, the CZEO-based supraMIC groups had slightly change on biomass, however, the biofilm cell viability decreased the CFU in 3 magnitude orders.
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OBJECTIVE: In Brazil, the most common method of controlling outbreaks of arbovirus is by the use of chemical sprays, which kill the insect vector, Aedes aegypti. The main objective of this study was to evaluate the resistance of Ae. aegypti to the insecticide, malathion, in situ. The location of this study was the municipality of Foz do Iguaçu, in the state of Paraná, Brazil. METHODS: Ultra-low-volume (ULV) fogging equipment was used, by vehicle, to apply the insecticide in situ, and mosquito populations after treatment were compared with those of control areas. The resistance of strains collected from the municipality was compared to the Rockefeller strain under laboratory conditions. RESULTS: We found 220 adult female specimens and 7423 eggs of Ae. aegypti in the areas subjected to UBV treatment, whereas 245 adult females and 10 557 eggs were found in the control areas. The UBV treatment area showed no significant difference compared to the control area, for all the indices. Mortality of the Rockefeller colony varied more quickly when there were slight variations in malathion concentration than the Foz do Iguaçu population.
OBJECTIF: Au Brésil, la méthode la plus courante de lutte contre les épidémies d'arbovirus consiste à utiliser des pulvérisations chimiques qui tuent l'insecte vecteur, Aedes aegypti. L'objectif principal de cette étude était d'évaluer la résistance de Ae. aegypti à l'insecticide, le malathion, in situ. Le lieu de cette étude était la municipalité de Foz do Iguaçu, dans l'état du Paraná, au Brésil. MÉTHODES: Un équipement de brumisation à très faible volume (ULV) a été utilisé, par véhicule, pour appliquer l'insecticide in situ et les populations de moustiques après le traitement ont été comparées à celles des zones témoins. La résistance des souches collectées dans la municipalité a été comparée à la souche Rockefeller dans des conditions de laboratoire. RÉSULTATS: Nous avons trouvé 220 spécimens femelles adultes et 7.423 Åufs d'Ae. aegypti dans les zones soumises au traitement ULV, alors que 245 femelles adultes et 10.557 Åufs ont été trouvés dans les zones témoins. La zone de traitement ULV n'a montré aucune différence significative par rapport à la zone témoin, pour tous les indices. La mortalité de la colonie de Rockefeller variait plus rapidement lorsqu'il y avait de légères variations dans la concentration de malathion que la population de Foz do Iguaçu.
Assuntos
Aedes/efeitos dos fármacos , Dengue/prevenção & controle , Resistência a Inseticidas , Inseticidas/farmacologia , Malation/farmacologia , Mosquitos Vetores/efeitos dos fármacos , Animais , Brasil/epidemiologia , Incidência , Controle de MosquitosRESUMO
In this paper, a semi-automatic multi-object tracking method to track a group of unmarked zebrafish is proposed. This method can handle partial occlusion cases, maintaining the correct identity of each individual. For every object, we extracted a set of geometric features to be used in the two main stages of the algorithm. The first stage selected the best candidate, based both on the blobs identified in the image and the estimate generated by a Kalman Filter instance. In the second stage, if the same candidate-blob is selected by two or more instances, a blob-partitioning algorithm takes place in order to split this blob and reestablish the instances' identities. If the algorithm cannot determine the identity of a blob, a manual intervention is required. This procedure was compared against a manual labeled ground truth on four video sequences with different numbers of fish and spatial resolution. The performance of the proposed method is then compared against two well-known zebrafish tracking methods found in the literature: one that treats occlusion scenarios and one that only track fish that are not in occlusion. Based on the data set used, the proposed method outperforms the first method in correctly separating fish in occlusion, increasing its efficiency by at least 8.15% of the cases. As for the second, the proposed method's overall performance outperformed the second in some of the tested videos, especially those with lower image quality, because the second method requires high-spatial resolution images, which is not a requirement for the proposed method. Yet, the proposed method was able to separate fish involved in occlusion and correctly assign its identity in up to 87.85% of the cases, without accounting for user intervention.
Assuntos
Algoritmos , Processamento de Imagem Assistida por Computador/métodos , Comportamento de Massa , Comportamento Espacial/fisiologia , Peixe-Zebra/fisiologia , Animais , Reprodutibilidade dos Testes , Gravação em VídeoRESUMO
Caryocar coriaceum fruits, found in Brazilian Cerrado and Caatinga, are commonly used as food and in folk medicine, as anti-inflammatory, bactericide, fungicide, leishmanicide, and nematicide. Due to the biological potential of this plant, this study focuses on the evaluation of antifungal and antileishmanial activities, including anticholinesterase and antioxidant tests, correlating with total phenols and flavonoids content. Peel extracts contain higher yield of phenols and flavonoids as analyzed by spectrophotometric methods. HPLC analysis of flavonoids revealed that isoquercitrin is the main flavonoid in both parts of the fruit, and peel extract showed the best antioxidant activity. In the inhibition of the acetylcholinesterase assay, both extracts demonstrate action comparable to physostigmine. The antimicrobial activity of extracts was evaluated against strains of Malassezia sp. and Microsporum canis, using the broth microdilution technique, in which the extracts showed similar MIC and MFC. The extracts present antileishmanial activity and low toxicity on murine macrophages and erythrocytes. Therefore, these results suggest a potential for the application of C. coriaceum fruit's ethanol extracts in the treatment against dermatophyte fungi and leishmaniasis, probably due to the presence of active flavonoids. Further in vivo studies are recommended aiming at the development of possible new pharmaceutical compounds.
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Several cases of animal and human salmonellosis caused by the Salmonella serotype Typhimurium have been reported. In animals, subclinical infection favors pathogen dissemination through feces. In this context, the domestic pigeon (Columba livia) with an asymptomatic condition may play an important role in the transmission of salmonellosis, through the elimination of contaminated feces in commercial aviaries or in poultry feed facilities, causing economic losses to the poultry industry and presenting a risk to public health. This study aimed to evaluate the mortality, clinical signs and the presence of Salmonella Typhimurium in the feces and organs of chicks previously inoculated with bacteria isolated from a pigeon. One-day-old chicks were distributed in two experimental groups (G1 and G2) of 32 birds each, and a control group of six birds. Two inocula of 0.4 and 0.7 mL with 105 and 106 colony forming units were used in G1 and G2 birds, respectively. At 1, 4, 7 and 14 days post-inoculation (dpi) fecal samples were pooled from each cage and individual cloacal swabs were collected. At 14 dpi, all chicks were euthanized and samples were collected from the liver, spleen, lung, cecum and intestine for microbiological analysis. Mortality was only observed among G2 birds (6.25%). Most birds presented clinical signs of diarrhea at 4 dpi and no symptom as observed at 14 dpi. The results
Na literatura são relatados diversos casos de salmonelose em animais e humanos em vários países ocasionados pelo sorotipo Typhimurium. Em animais, a infeção subclínica favorece a disseminação do patógeno através das fezes. Nesse contexto, o pombo (Columba livia) com quadro assintomático pode desempenhar um importante papel na transmissão de salmonelose, mediante a excreção de fezes contaminadas em aviários comerciais ou em instalações de ração animal causando perdas econômicas para a indústria avícola, e proporcionando risco para a saúde pública. Objetivou-se avaliar a taxa de mortalidade, sinal clínico e a presença de Salmonella Typhimurium nas fezes e nos órgãos de pintinhos previamente inoculados com bactérias oriundas de pombo. Foram utilizados 70 pintos de corte de um dia de idade, os quais foram distribuídos aleatoriamente em dois grupos (G1 e G2) de 32 aves cada. Utilizou-se um grupo controle, constituído por seis aves, antes do início do tratamento para confirmação da ausência do patógeno. Utilizou-se inóculos de 0,4 e 0,7 mL contendo as concentrações de 105 e 106 unidades formadoras de colônias nas aves do grupo 1 (G1) e grupo 2 (G2), respectivamente. Nos dias 1, 4, 7 e 14 pós inoculação (dpi) foram coletados pool de fezes de cada gaiola, como também, swabs cloacais individuais. Ao 14 dpi, todos os pintos foram eutanasiados e coletados fígado, baço, pulmão, ceco e int
RESUMO
Several cases of animal and human salmonellosis caused by the Salmonella serotype Typhimurium have been reported. In animals, subclinical infection favors pathogen dissemination through feces. In this context, the domestic pigeon (Columba livia) with an asymptomatic condition may play an important role in the transmission of salmonellosis, through the elimination of contaminated feces in commercial aviaries or in poultry feed facilities, causing economic losses to the poultry industry and presenting a risk to public health. This study aimed to evaluate the mortality, clinical signs and the presence of Salmonella Typhimurium in the feces and organs of chicks previously inoculated with bacteria isolated from a pigeon. One-day-old chicks were distributed in two experimental groups (G1 and G2) of 32 birds each, and a control group of six birds. Two inocula of 0.4 and 0.7 mL with 105 and 106 colony forming units were used in G1 and G2 birds, respectively. At 1, 4, 7 and 14 days post-inoculation (dpi) fecal samples were pooled from each cage and individual cloacal swabs were collected. At 14 dpi, all chicks were euthanized and samples were collected from the liver, spleen, lung, cecum and intestine for microbiological analysis. Mortality was only observed among G2 birds (6.25%). Most birds presented clinical signs of diarrhea at 4 dpi and no symptom as observed at 14 dpi. The results
Na literatura são relatados diversos casos de salmonelose em animais e humanos em vários países ocasionados pelo sorotipo Typhimurium. Em animais, a infeção subclínica favorece a disseminação do patógeno através das fezes. Nesse contexto, o pombo (Columba livia) com quadro assintomático pode desempenhar um importante papel na transmissão de salmonelose, mediante a excreção de fezes contaminadas em aviários comerciais ou em instalações de ração animal causando perdas econômicas para a indústria avícola, e proporcionando risco para a saúde pública. Objetivou-se avaliar a taxa de mortalidade, sinal clínico e a presença de Salmonella Typhimurium nas fezes e nos órgãos de pintinhos previamente inoculados com bactérias oriundas de pombo. Foram utilizados 70 pintos de corte de um dia de idade, os quais foram distribuídos aleatoriamente em dois grupos (G1 e G2) de 32 aves cada. Utilizou-se um grupo controle, constituído por seis aves, antes do início do tratamento para confirmação da ausência do patógeno. Utilizou-se inóculos de 0,4 e 0,7 mL contendo as concentrações de 105 e 106 unidades formadoras de colônias nas aves do grupo 1 (G1) e grupo 2 (G2), respectivamente. Nos dias 1, 4, 7 e 14 pós inoculação (dpi) foram coletados pool de fezes de cada gaiola, como também, swabs cloacais individuais. Ao 14 dpi, todos os pintos foram eutanasiados e coletados fígado, baço, pulmão, ceco e int
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OBJECTIVE: This study evaluated the stress distribution by loads, under a internal connection implant system with two sort of abutment screws and prosthetic crown models at ten observation points. MATERIALS AND METHOD: The analysis were made in two models with internal butt joint, and with gold and titanium screw, respectively. The load was 382N with 90 masculine to the occlusal surface and 15 masculine to the implant axis at 4 and 6 mm from the implant center. RESULTS: In both models, a large amount of stress was located around the implant neck and little stress was concentrated along the abutment screw. CONCLUSION: The simulations made suggest that the internal connection protects the abutment screw from the accumulated stresses; however, it exposes the implant walls to these stresses.
Assuntos
Dente Suporte , Implantes Dentários , Prótese Dentária Fixada por Implante , Análise de Elementos Finitos , Imageamento Tridimensional/métodos , Ligas , Silicatos de Alumínio/química , Fenômenos Biomecânicos , Simulação por Computador , Desenho Assistido por Computador , Coroas , Ligas Dentárias/química , Materiais Dentários/química , Porcelana Dentária/química , Planejamento de Prótese Dentária , Ligas de Ouro/química , Humanos , Teste de Materiais , Modelos Biológicos , Compostos de Potássio/química , Estresse Mecânico , Propriedades de Superfície , Titânio/químicaRESUMO
BACKGROUND AND OBJECTIVES: The Nd:YAG laser can be used in Dentistry to remove soft tissue, disinfect canals in endodontic procedures and prevent caries. However, there is no protocol for Nd:YAG laser application in dental bleaching. The aims of this in vitro study were: (a) to observe the tooth shade alteration when hydrogen peroxide whitening procedures are associated with dyes with different wavelengths and irradiated with Nd:YAG laser or halogen light; (b) to measure the Vickers (VHN) enamel microhardness before and after the whitening procedure; (c) to evaluate the tensile bond strength of two types of adhesive systems applied on bleached enamel; (d) to observe the failure pattern after bond strength testing; (e) to evaluate the pulpal temperature during the bleaching procedures with halogen light or laser; (f) to measure the kinetic reaction of hydrogen peroxide. MATERIALS AND METHODS: Extracted sound human molar crowns were sectioned in the mesiodistal direction to obtain 150 fragments that were divided into five groups for each adhesive system: WL (H(2)O(2) + thickener and Nd:YAG), WH (H(2)O(2) + thickener and halogen light), QL (H(2)O(2) + carbopol + Q-switch and Nd:YAG), QH (H(2)O(2) + carbopol + Q-switch and halogen light), and C (Control, without whitening agent). Shade assessment was made with a shade guide and the microhardness tests were performed before and after the bleaching procedures. Immediately afterwards, the groups were restored with the adhesive systems Adper Single Bond 2 or Solobond M plus composite resin, and the tensile bond strength test was performed. The temperature was measured by thermocouples placed on the enamel surface and intrapulpal chamber. The kinetics of hydrogen peroxide was observed by ultraviolet analysis. RESULTS: The shade changed seven levels for Nd:YAG laser groups and eight levels for halogen light. According to the student's t-test, there was no statistical difference between the VHN before and after the whitening protocols (p > 0.05). The tensile bond strength showed no statistical significance between the test groups and the controls, considering both adhesive systems tested by ANOVA and Tukey tests (p > 0.05). The predominant failure pattern after bond strength testing was mixed. The temperature was safe for laser and halogen light. The kinetic reaction showed that after 5 min all the hydrogen peroxide had been consumed. CONCLUSIONS: Nd:YAG laser associated with hydrogen peroxide bleached the enamel, the shade being similar to that obtained with the traditional method performed with halogen light. Moreover, the Vickers' microhardness and bond strength values were not altered in comparison with those for nonbleached enamel.
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Esmalte Dentário , Cor , Corantes , Peróxido de Hidrogênio/química , Cinética , Lasers de Estado Sólido , Microscopia Eletrônica de Varredura , Neodímio , Resistência à Tração , Clareamento DentalRESUMO
The purposes of this study were to develop a device to measure bracket debonding force in vivo and to evaluate, in vitro, the bond strength obtained with the device and with tensile and shear bond strength (SBS) tests performed in a universal testing machine. The device was developed using polypropylene pliers (3M Unitek). The basic principle consisted of measuring the applied force to debond, using two strain gauges (Kyowa) bonded to the region of major deformation of the plier handles. The crowns of 75 bovine incisors were embedded in acrylic resin and orthodontic brackets were bonded to the facial surface with Transbond XT (3M Unitek). In group A (n = 25) debonding was carried out with the device, while tensile bond strength testing was performed in group B (n = 25) and SBS testing in group C (n = 25). A universal testing machine (EMIC-DL-2,000) was used for these last two groups. According to analysis of variance and Tukey's test (alpha = 0.05), the mean bond strength for group C (7.71 MPa) was statistically higher than for groups A (2.98 MPa) and B (2.69 MPa). Groups A and B were not statistically different. The device was shown to be feasible to obtain in vivo bond strength values for orthodontic brackets, and that the bond strength values were dependent on the method and direction of debonding.