RESUMO
BACKGROUND: The use of nanobiomaterials is increasing each day. Among the immense variety of nanomaterials developed and studied the hydroxyapatite is one of the most ones. OBJECTIVE: In this study we developed and tested nano-hydroxyapatite dopped with Ho-166 for bone cancer. RESULTS: The results showed that the nano-hydroxyapatite dopped with Ho-166 has a great affinity for the bone. CONCLUSION: The pre-clinical studies support the use as a nano-radiopharmaceuticals for bone cancer treatment and diagnosis.
Assuntos
Antineoplásicos/uso terapêutico , Neoplasias Ósseas/tratamento farmacológico , Sistemas de Liberação de Medicamentos , Durapatita/química , Hólmio/química , Nanoestruturas/química , Compostos Radiofarmacêuticos/uso terapêutico , Animais , Antineoplásicos/síntese química , Antineoplásicos/química , Neoplasias Ósseas/diagnóstico , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Feminino , Masculino , Estrutura Molecular , Compostos Radiofarmacêuticos/síntese química , Compostos Radiofarmacêuticos/química , Ratos , Ratos Wistar , Relação Estrutura-AtividadeRESUMO
The use of in vivo assay to determine the biodistribution and subsequent inter-comparison with human parameters has been used since the dawn of science. The use of this type of test admits the metabolic equity among animals for inter-comparison. Thus, the use of Wistar rats in particular is quite frequent. Regarding routes of administration, there are three ways to test priority: jugular vein, intraocular (eye plexus) and caudal; there is a consensus that these three pathways behave in the same way, or at least very similar. Biodistribution studies of drugs, especially radiopharmaceuticals, have been using randomly any of these pathways believed to be effective in their likeness without worrying about your real analytic equity. In this study, we performed in vivo assay in 8 Wistar rats using 99mTc -labeled Herceptin to review the route of administration on the biodistribution result. Thus, four mice were injected via the intraocular (eye plexus), and four were injected via tail (caudal plexus). The results were quite disparate and call the attention of the scientific community to reassess the protocols for animal experiments, in order to have uniformity and fairness between the data and may represent a test for human inter-comparison of more reliable and trustworthy way.