RESUMO
OBJECTIVE: To compare the proliferation and the expression of matrix metalloproteinases (MMPs; MMP-2 and MMP-9) in solid and unicystic ameloblastomas with ameloblastic carcinomas. STUDY DESIGN: Five cases of ameloblastic carcinoma (AC), 18 cases of solid ameloblastoma (SA), and seven of unicystic ameloblastoma (UA) were selected. The immunohistochemical expression of MMPs was assessed by the percentage of positive tumor cells and stained stroma. The mean argyrophilic nucleolar organizer region (AgNOR) and the percentage of cells with more than one AgNOR per nucleus were evaluated. RESULTS: Statistically significant higher mean AgNOR was observed in AC than in SA and UA. MMP-2 was expressed similarly in tumor and stroma among groups. MMP-9 was higher in the stroma of SA than that of UA (P = .0484). CONCLUSIONS: The cell proliferation was related to the greater aggressiveness of AC. High expression of MMP-2 and MMP-9 in all lesions highlighted the importance of these enzymes in the biology of ameloblastic tumors.
Assuntos
Ameloblastoma/enzimologia , Neoplasias Mandibulares/enzimologia , Metaloproteinase 2 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/metabolismo , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Ameloblastoma/patologia , Biomarcadores Tumorais/metabolismo , Brasil , Proliferação de Células , Criança , Estudos Transversais , Feminino , Humanos , Masculino , Neoplasias Mandibulares/patologia , Pessoa de Meia-Idade , Estudos RetrospectivosAssuntos
Carcinoma de Células Escamosas/diagnóstico , Neoplasias do Seio Maxilar/diagnóstico , Adulto , Biópsia , Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/cirurgia , Diagnóstico Diferencial , Humanos , Imuno-Histoquímica , Masculino , Neoplasias do Seio Maxilar/patologia , Neoplasias do Seio Maxilar/cirurgia , Radiografia Panorâmica , Tomografia Computadorizada por Raios XRESUMO
Tufted angioma (TA) is a benign vascular tumor with endothelial origin. It is extremely rare in oral mucosa; only seven cases have been reported in the literature so far. Here, we describe two cases of tufted angioma observed in children and we also present a review of the literature about this pathology, concerning the differential diagnosis and management of this lesion in children.
RESUMO
Tumoral invasion process is the result of a complex interaction between the tumor cells and microenvironment which plays an important role in modulating the growth and invasion of the cancer. The myoepithelial cells, present in glandular organs such as the breast and salivary glands, seem to exert paracrine effects on the glandular epithelium, acting as natural tumor suppressors. To verify the influence of the benign myoepithelial cells in the invasion of malignant cells, simulating an in situ carcinoma ex pleomorphic adenoma, we have cultured three different high-potential invasive malignant tumors (breast ductal adenocarcinoma, melanoma and oral squamous cell carcinoma) in conditioned medium of myoepithelial cells from salivary gland pleomorphic adenomas using transwell chambers with 8-µm pores membrane coated with matrigel. After 96 h, quantitative analyses of the results were performed by calculating the invasion index (number of cells that invaded in relation to the total number of cells). The results showed that there was a reduction of the invasion index mean for the three different malignant tumors. This study supports a tumoral suppressor function of the myoepithelial cells from pleomorphic adenoma in in vitro invasion process.
Assuntos
Adenoma Pleomorfo/patologia , Meios de Cultivo Condicionados , Invasividade Neoplásica/patologia , Actinas/análise , Materiais Biocompatíveis/química , Neoplasias da Mama/patologia , Proteínas de Ligação ao Cálcio/análise , Carcinoma in Situ/patologia , Carcinoma Ductal de Mama/patologia , Carcinoma de Células Escamosas/patologia , Técnicas de Cultura de Células , Linhagem Celular Tumoral , Técnicas de Cocultura , Colágeno/química , Cultura em Câmaras de Difusão , Combinação de Medicamentos , Células Epiteliais/metabolismo , Feminino , Humanos , Laminina/química , Melanoma/patologia , Membranas Artificiais , Proteínas dos Microfilamentos/análise , Filtros Microporos , Neoplasias Bucais/patologia , Proteínas Musculares/análise , Miócitos de Músculo Liso/metabolismo , Proteoglicanas/química , Microambiente Tumoral , Vimentina/análise , CalponinasRESUMO
Pyogenic granuloma (PG) is a benign inflammatory lesion, nonneoplastic in nature, which occurs in the oral cavity and skin. This lesion arises in response to various stimuli such as low-grade local irritations, traumatic injury, or hormonal factors. Recently, in some cases, the occurrence of recurrent PGs in skin associated with vascular lesions, such as port-wine stains, has been described. It has been postulated that this association is promoted by arteriovenous anastomoses in the vascular lesions, leading to the development of PG. The authors discuss 2 cases of recurrent PG in patients with a port-wine stain, and the treatment options adopted.