RESUMO
We evaluated the biological effects of ingestion by gavage, for 28 days, of the pesticides cypermethrin (CP) and emamectin benzoate (EB) and their mixtures in female Swiss mice. The groups were Control (water); CP; EB and three distinct concentrations of CP and EB mixture expressed in mg/kg/day. The biological effects were analyzed in the complete blood count and plasma (alkaline phosphatase (ALP), alanine aminotransferase (ALT) and creatinine); the biochemical parameters of oxidative stress (substances reactive to thiobarbituric acid (TBARS); reduced glutathione (GSH); catalase (CAT), superoxide dismutase (SOD) and glutathione-S-transferase (GST)), and bone marrow cells obtained from the femur for the micronucleus (MN) test. In the heart, there was a reduction in GSH in the groups (0.5 + 0.67 and 2.5 + 3.37), although in the brain this effect appeared for the other groups, except EB. Brain TBARS increased in CP and in the group (2.5 + 3.37) and platelets increased in the group (12.5 + 16.87). Genotoxic/mutagenic effects, showing a consistent increase dose-dependent effect on micronucleus counting for in the female mice. After 28 days of treatment, we can observe that the pesticide mixtures promoted genotoxic damage and oxidative brain damage in female mice, which can damage the health of these animals and possibly their future offspring.
Assuntos
Mutagênicos , Praguicidas , Animais , Antioxidantes/metabolismo , Benzoatos/farmacologia , Catalase/metabolismo , Dissacarídeos , Feminino , Glutationa/metabolismo , Glutationa Transferase/genética , Glutationa Transferase/metabolismo , Ivermectina/análogos & derivados , Fígado , Camundongos , Estresse Oxidativo , Praguicidas/toxicidade , Piretrinas , Superóxido Dismutase/metabolismo , Substâncias Reativas com Ácido TiobarbitúricoRESUMO
Roundup Original® is an herbicide widely used in Mato Grosso's agriculture and it may contamine water bodies, being an unforeseen xenobiotic to aquatic organisms, particularly fish. This study investigated the effects on the hybrid fish jundiara (Leiarius marmoratus×Pseudoplatystoma reticulatum) of an environmentally relevant exposure to this herbicide. Glucose levels in liver, muscle and plasma decreased after exposure to 1.357mgL-1 of Roundup Original® (glyphosate nominal concentration), while glycogen levels reduced in liver and muscle for different times. Elevated cholesterol and triglycerides revealed an adaptive response. Protein and lactate levels also increased during the experiment, however no changes were observed for muscle lactate. Increment of the transaminases suggests damage to the liver cells. After 96hours of exposure, reductions in all hematological parameters were observed, whereas the micronucleus test findings showed genotoxic scenery. Histological analysis did not display pathological alterations of the hepatic tissue. The results obtained provide valuable data for noticing the effects of pollutants on non-target organisms.