RESUMO

Cyclin B is a regulatory subunit of maturation-promoting factor (MPF), which has a key role in the induction of meiotic maturation of oocytes. MPF has been studied in a wide variety of animal species; however, its expression in crustaceans is poorly characterized. In this study, the complete cDNA sequence of Cyclin B was cloned from the red claw crayfish, Cherax quadricarinatus, and its spatiotemporal expression profiles were analyzed. Cyclin B cDNA (1779 bp) encoded a 401 amino acid protein with a calculated molecular weight of 45.1 kDa. Quantitative real-time PCR demonstrated that Cyclin B mRNA was expressed mainly in the ovarian tissue and that the expression decreased as the ovaries developed. Immunofluorescence analysis revealed that the Cyclin B protein relocated from the cytoplasm to the nucleus during oogenesis. These findings suggest that Cyclin B plays an important role in gametogenesis and gonad development in C. quadricarinatus.

Assuntos

Astacoidea/genética , Ciclina B/genética , Regulação da Expressão Gênica no Desenvolvimento , Fator Promotor de Maturação/genética , Oócitos/metabolismo , Oogênese/genética , Sequência de Aminoácidos , Animais , Astacoidea/citologia , Astacoidea/crescimento & desenvolvimento , Sequência de Bases , Núcleo Celular/metabolismo , Clonagem Molecular , Ciclina B/metabolismo , Citoplasma/metabolismo , DNA Complementar/genética , DNA Complementar/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Feminino , Fator Promotor de Maturação/metabolismo , Meiose , Dados de Sequência Molecular , Peso Molecular , Oócitos/citologia , Oócitos/crescimento & desenvolvimento , Fases de Leitura Aberta , Ovário/citologia , Ovário/crescimento & desenvolvimento , Ovário/metabolismo , Transporte Proteico , Reação em Cadeia da Polimerase em Tempo Real , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Alinhamento de Sequência