RESUMO

Osteosarcoma is one of the most common primary bone tumors in children and young adults. In this study, we investigated the role of musculoaponeurotic fibrosarcoma oncogene homolog K (MAFK) in osteosarcoma cell proliferation in vitro and the possible pathways that contributed to MAFK-related osteosarcoma development. We first reported that MAFK was expressed at low levels in an osteosarcoma cell line. Furthermore, a significant correlation between MAFK and the Wnt signaling pathway was observed in osteosarcoma by using a gene microarray assay. We found that expression of MAFK could be induced by Wnt1 in a dose-dependent manner. Furthermore, Wnt1-induced MAFK expression caused a significant increase of cell viability, whereas a Wnt pathway inhibitor, IWR-1-endo, abolished Wnt1-induced effects on MAFK. Finally, cell cycle analysis showed that enhanced cell proliferation might be attributed to re-distribution of the cell cycle. Together, our results suggested that Wnt1-induced MAFK expression promoted cell proliferation in MG63 cells, and that the role of MAFK in osteosarcoma might be closely linked to the Wnt signaling pathway.

Assuntos

Neoplasias Ósseas/metabolismo , Neoplasias Ósseas/patologia , Fator de Transcrição MafK/biossíntese , Osteossarcoma/metabolismo , Osteossarcoma/patologia , Proteína Wnt1/metabolismo , Neoplasias Ósseas/genética , Linhagem Celular Tumoral , Proliferação de Células/fisiologia , Humanos , Fator de Transcrição MafK/genética , Osteossarcoma/genética , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Transfecção , Via de Sinalização Wnt , Proteína Wnt1/genética