RESUMO

An improved RNA interference method was developed in Trichoderma reesei, using convergent dual promoters for efficient and high-throughput RNA silencing. This new vector allowed for the silencing of the eGFP gene and target genes to occur simultaneously, significantly facilitating the rapid screening of the transformants using eGFP as a reporter.

Assuntos

Marcação de Genes/métodos , Interferência de RNA , Trichoderma/genética , Celulose/metabolismo , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Regiões Promotoras Genéticas , Transformação Genética , Trichoderma/metabolismo