RESUMO
This study investigated the anti-inflammatory effect of hydrophilic and lipophilic extracts from juçara fruits (Euterpe edulis Martius) through measurement of nitric oxide (NOx) and cytokines (IL-12p70, TNF-α, INF-γ, MCP-1, IL-6, and IL-10). J774 macrophages were stimulated with lipopolysaccharides (1 µg/mL) and treated with various concentrations (1-100 µg/mL) of juçara fruits extracts from crude extracts, and hexane, dichloromethane, ethyl acetate, and butanol fractions. Potential relationships between the phenolic composition of the extracts determined by LC-ESI-MS/MS and their anti-inflammatory capacity were also evaluated. Hexane and dichloromethane fractions inhibited NOx and IL-12p70 while increased IL-10. Hexane fractions also decreased IL-6 and IFN-γ production. Hexane and dichloromethane fractions showed a higher number of phenolic compounds (32 and 34, respectively) than the other extracts tested and were also the only ones that presented benzoic acid and pinocembrin. These results suggest juçara fruits compounds as potential anti-inflammatory agents, especially those of a more apolar nature.
Assuntos
Anti-Inflamatórios , Frutas , Lipopolissacarídeos , Macrófagos , Óxido Nítrico , Extratos Vegetais , Macrófagos/efeitos dos fármacos , Frutas/química , Extratos Vegetais/farmacologia , Anti-Inflamatórios/farmacologia , Óxido Nítrico/metabolismo , Camundongos , Animais , Fenóis/análise , Fenóis/farmacologia , Citocinas/metabolismo , Linhagem CelularRESUMO
Euterpe edulis is an endangered palm species that provides the most important non-timber forest product exploited in its natural habitat, the Brazilian Atlantic Forest hotspot1,4. From 1991 to 2017, pasturelands, agriculture, and monoculture of tree plantations were responsible for 97% of Atlantic Forest deforested areas in Brazil and Santa Catarina was one of the Brazilian states with the greatest loss of forest area14. In the last decade, E. edulis fruits reached their highest commercial value, producing the southeastern equivalent of Amazonian ''açai'' (Euterpe oleracea)5,7,8. As a shade-tolerant species, E. edulis adapts very well to agroforestry systems8,10. To evaluate potential areas for cultivation of E. edulis through agroforestry systems, we developed and applied a spatial model for mapping suitable areas. To accomplish this, we analyzed multisource biophysical data and E. edulis distribution data from the Forest Inventory of Santa Catarina. We identified two areas with potential occurrence of the species, one in the domains of coastal Dense Ombrophilous Forest where the species is more common and another in the domains of inland Deciduous Seasonal Forest where its occurrence was suspected, but not proven, until 2021. Today, Deciduous Seasonal Forest is the most fragmented and impacted by agriculture. Our model, together with confirmed areas of occurrence, indicates that deciduous seasonal forest region should be prioritized for production and recovery of E. edulis through agroforestry systems.