RESUMO
Corynebacterium pseudotuberculosis is a pathogen of great veterinary and economic importance, since it affects livestock, mainly sheep and goats, worldwide, together with reports of its presence in camels in several Arabic, Asiatic, and East and West African countries, as well as Australia. In this article, we report the genome sequence of Corynebacterium pseudotuberculosis strain Cp162, collected from the external neck abscess of a camel in the United Kingdom.
Assuntos
Corynebacterium pseudotuberculosis/genética , DNA Bacteriano/química , DNA Bacteriano/genética , Genoma Bacteriano , Análise de Sequência de DNA , Abscesso/microbiologia , Abscesso/veterinária , Animais , Camelus , Infecções por Corynebacterium/microbiologia , Infecções por Corynebacterium/veterinária , Corynebacterium pseudotuberculosis/isolamento & purificação , Dados de Sequência Molecular , Reino UnidoRESUMO
Prokaryotes commonly present outer cell wall structures composed of a crystalline array of proteinaceous subunits, known as surface layers (S-layers). The ORF encoding the S-layer protein (SlpA) of Lactobacillus brevis was cloned into Lactococcus lactis under the transcriptional control of the xylose-inducible expression system (XIES). SlpA was secreted into the extracellular medium, as determined by immunoblotting, and assays on the kinetics of SlpA production revealed that repression of the system with glucose did not require the depletion of xylose from the medium that allows transitory ORF expression. The successful use of XIES to express S-layer proteins in the versatile and generally recognized as safe species L. lactis opens new possibilities for an efficient production and isolation of SlpA S-layer protein for its various applications in biotechnology and importantly as an antigen-carrying vehicle.
Assuntos
Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/metabolismo , Lactococcus lactis/genética , Lactococcus lactis/metabolismo , Levilactobacillus brevis/genética , Proteínas de Bactérias/genética , Western Blotting , Clonagem Molecular , Meios de Cultura/química , Expressão Gênica , Regulação Bacteriana da Expressão Gênica , Regulação Enzimológica da Expressão Gênica , Glucose/metabolismo , Xilose/metabolismoRESUMO
In this work, we report the complete genome sequence of a Corynebacterium pseudotuberculosis PAT10 isolate, collected from a lung abscess in an Argentine sheep in Patagonia, whose pathogen also required an investigation of its pathogenesis. Thus, the analysis of the genome sequence offers a means to better understanding of the molecular and genetic basis of virulence of this bacterium.
Assuntos
Infecções por Corynebacterium/veterinária , Corynebacterium pseudotuberculosis/genética , Genoma Bacteriano , Abscesso Pulmonar/microbiologia , Doenças dos Ovinos/microbiologia , Animais , Argentina , Sequência de Bases , Corynebacterium pseudotuberculosis/isolamento & purificação , Corynebacterium pseudotuberculosis/patogenicidade , Dados de Sequência Molecular , Ovinos , VirulênciaRESUMO
In this work, we report the whole-genome sequence of Corynebacterium pseudotuberculosis bv. equi strain CIP 52.97 (Collection Institut Pasteur), isolated in 1952 from a case of ulcerative lymphangitis in a Kenyan horse, which has evidently caused significant losses to agribusiness. Therefore, obtaining this genome will allow the detection of important targets for postgenomic studies, with the aim of minimizing problems caused by this microorganism.