RESUMO
A new purified antigen (E2) of Paracoccidioides brasiliensis mycelial growth phase was isolated by immunoadsorption from a crude metabolic soluble extract of the fungus. The antiserum prepared in a rabbit by inoculation of E2 antigen developed only one immunodiffusion line with the crude metabolic extract. Findings on immunological analysis showed that E2 antigen is the antigenic component of immunoelectrophoretic band E. The isolated antigens did not possess detectable alkaline phosphatase activity. It reacted in immunodiffusion tests with all the sera (14/14) from P. brasiliensis infected patients containing precipitating antibodies.
Assuntos
Antígenos de Fungos , Fungos/imunologia , Paracoccidioides/imunologia , Antígenos de Fungos/análise , Antígenos de Fungos/isolamento & purificação , Reações Cruzadas , Histoplasmose/imunologia , Humanos , Imunodifusão , Imunoeletroforese , Paracoccidioidomicose/imunologiaRESUMO
A specific antigen of Paracoccidioides brasiliensis was isolated from a metabolic extract of the fungus. The extraction was made by specific adsorption to and subsequent elution from a column containing a cross linked polymer to which the antibodies of a monospecific rabbit serum had been covalently attached. The purity of the final product was demonstrated by immunodiffusion analysis of the eluate using immune serum produced in a sensitized rabbit. The purified antigen was shown to have cationic electrophoretic mobility and alkaline phosphatase activity.
Assuntos
Fosfatase Alcalina/análise , Antígenos de Fungos/isolamento & purificação , Fungos/imunologia , Paracoccidioides/imunologia , Animais , Soros Imunes , Imunoeletroforese , Paracoccidioides/enzimologia , CoelhosAssuntos
Equinococose Pulmonar/diagnóstico , Adolescente , Adulto , Criança , Chile , Equinococose Pulmonar/imunologia , Feminino , Humanos , Imunoeletroforese , Masculino , Pessoa de Meia-IdadeRESUMO
The immunoelectrophoretic reactivity of a "crude" and a "purified" antigens of Paracoccidiodes brasiliensis against the sera of confirmed cases of paracoccidioidomycosis and histoplasmosis was evaluated. The "crude" antigen revealed precipitating antibodies in both the homologous and the heterologous human sera; the number of precipitating systems being higher with the former. The "purified" antigen reacted only with the homologous sera. It is postulated that the use of such "purified" antigen may significantly reduce the non specificity of the serologic tests in the immunodiagnosis of paracoccidioidomycosis.