RESUMO
Background: Buffalo breeding is common in many countries. Buffalo's milk is used in the production of mozzarella, yoghurt, ice cream, and various dairy desserts; meat is preferred in sausage production. The female buffaloes are bred to benefit from their milk and to obtain offspring. These animals, which are not suitable for feeding in barns, generally live in pastures, especially on wet land, and are very difficult to follow. Therefore, diseases occur randomly in slaughterhouses. Studies on genital system problems are very limited. Water buffaloes (Bubalus bubalis) are animals with economic value as many buffalo products are provided. In this respect, inspection and control of female genital disorders is fundamental to ensure good reproductive performance of female buffaloes. The aim of the study was to investigate pathomorphological lesions occurring in the ovaries of water buffaloes which were sent to slaughterhouses. Materials, Methods & Results: A total of 198 ovaries of water buffaloes were collected from various slaughterhouses located in Adapazari, Istanbul, Kocaeli, Samsun and Trabzon cities of Turkey. After macroscopic examination; tissue samples were fixed in 10 % buffered formalin, processed routinely and were stained with hematoxylin-eosin (HE). As a histochemical staining, Masson's trichrome staining was applied to characterize the lesions. Immunohistochemistry (IHC) was performed on 10 % neutral formalin fixed, paraffin-embedded, 4-6-µm-thick sections from ovaries using progesterone receptor (PR) with streptavidin-biotin complex peroxidase (StrepABC-P) method. Histopathologically, follicular cysts (n:147) and luteal cysts (n:22) were seen. While the cyst lumens were sometimes surrounded by granulosa and/or luteal cells, most of them were limited by the connective tissue capsule structure. This capsule structure was shown in blue by Masson's trichrome staining. Hemorrhage was observed in some cystic corpus luteum, diagnosed as corpus hemorrhagicum. In addition, cysts giving papillary extension into the lumen and inflammation of some ovaries were observed. Immunohistochemically, the staining with PR antibody in ovarian cysts showed no immunolabelling around the follicular cyst, while the nuclei of some of the luteal cells forming the luteal cyst had strongly nuclear positivity and slightly cytoplasmic positivity. In the biochemical examination of the fluids obtained from cystic ovaries (n: 37), the average of estradiol was 2.84 ng/mL (min: 0.01 ng/mL, max: 4.30 ng/mL) and progesterone average is 49.09 ng/mL (min: 1.88 ng/mL, max: 254.2 ng/mL). Discussion: Ovarian cysts in buffaloes seem to be among the serious fertility problems as in cattle. Although the exact cause of ovarian cyts has not been determined yet, it is known that the hypothalamic-pituitary-gonadal (HPG) axis is disrupted in cyst formation in general. In this study, the mean estradiol value was within the standard range but close to the lower limit; progesterone value was above the limit. The increase in progesterone level was compatible with the pathogenesis of cyst genesis. Beside this result, staining with PR was positive in the luteal cells that formed the luteal cyst immunohistochemically. In addition, although the hemorrhages observed in the corpus luteum are considered physiological, it should not be forgotten that they can be vital if they rupture. All these results show us the animals sent for slaughtering mostly have serious genital problems threatening their fertility.
Assuntos
Animais , Feminino , Cistos Ovarianos/veterinária , Ovário/lesões , Búfalos/lesões , Imuno-Histoquímica/veterinária , Infertilidade Feminina/prevenção & controleRESUMO
Background: Urolithiasis is one of the important lower urinary tract diseases in dogs. Uroliths develop when urine becomessupersaturated. Struvite urolithiasis in dogs is formed when urine saturated with magnesium, ammonium and phosphate.Renal papillary necrosis is a form of nephropathy involving the necrosis of the renal papilla. The aim of this case is theevaluation of histopathological changes in kidney and urinary bladder caused by urolithiasis with sitruvite, a pathologycommonly seen in dogs. Renal papillary necrosis that encountered in the case was evaluated histopathologically. We intendto discuss the relationship between urolithiasis and renal papillary necrosis.Case: The material of the case was a 7.5 year-old Rottweiler female dog that had been operated for the urolithiasis treatment and died after surgery. The animal sent to pathology department was necropsied and evaluated histopathologically.Also the uroliths removed during the surgery were sent for analysis postoperatively. Qualitative composition of urolithswas determined by a series of chemical analyses in Department of Biochemistry. X-ray diffraction was performed as wellin General Directorate of Mineral Research and Exploration in the objective of result verification. Macroscopically, 30pieces uroliths with varying size from 1 mm to 3 cm were seen in urinary bladder. They were 110 g in weight with smoothsurface and round and/or ovoid shapes. After necropsy, specimens from kidney and urinary bladder were fixed in 10%neutral buffered formalin, embedded in paraffin wax, cut at 5 μm, and stained with hematoxylin and eosin (HE). Bladderwall thickening and hemorrhage on mucosa were noticed. Kidneys had roughened surface with the capsule being difficultto peel off. Also, renal papillary necrosis, reddish in color was observed in papilla of the right kidney. Microscopically,crystal formations were observed proximal tubule lumens of kidneys. Interstitial nephritis...(AU)
Assuntos
Animais , Feminino , Cães , Urolitíase/complicações , Urolitíase/veterinária , Necrose Papilar Renal/veterinária , EstruvitaRESUMO
Background: Urolithiasis is one of the important lower urinary tract diseases in dogs. Uroliths develop when urine becomessupersaturated. Struvite urolithiasis in dogs is formed when urine saturated with magnesium, ammonium and phosphate.Renal papillary necrosis is a form of nephropathy involving the necrosis of the renal papilla. The aim of this case is theevaluation of histopathological changes in kidney and urinary bladder caused by urolithiasis with sitruvite, a pathologycommonly seen in dogs. Renal papillary necrosis that encountered in the case was evaluated histopathologically. We intendto discuss the relationship between urolithiasis and renal papillary necrosis.Case: The material of the case was a 7.5 year-old Rottweiler female dog that had been operated for the urolithiasis treatment and died after surgery. The animal sent to pathology department was necropsied and evaluated histopathologically.Also the uroliths removed during the surgery were sent for analysis postoperatively. Qualitative composition of urolithswas determined by a series of chemical analyses in Department of Biochemistry. X-ray diffraction was performed as wellin General Directorate of Mineral Research and Exploration in the objective of result verification. Macroscopically, 30pieces uroliths with varying size from 1 mm to 3 cm were seen in urinary bladder. They were 110 g in weight with smoothsurface and round and/or ovoid shapes. After necropsy, specimens from kidney and urinary bladder were fixed in 10%neutral buffered formalin, embedded in paraffin wax, cut at 5 μm, and stained with hematoxylin and eosin (HE). Bladderwall thickening and hemorrhage on mucosa were noticed. Kidneys had roughened surface with the capsule being difficultto peel off. Also, renal papillary necrosis, reddish in color was observed in papilla of the right kidney. Microscopically,crystal formations were observed proximal tubule lumens of kidneys. Interstitial nephritis...