RESUMO
We isolated and characterized 22 polymorphic microsatellite loci in the crimson snapper (Lutjanus erythropterus) using a (GT)13-enriched genomic library. We found three to 15 alleles per locus, with a mean of 6.68. The observed and expected heterozygosities ranged from 0.087 to 0.978 and from 0.125 to 0.904, respectively, with averages of 0.576 and 0.650, respectively. Only three loci showed significant deviation from the Hardy-Weinberg equilibrium after Bonferroni correction. Four loci showed evidence for null alleles. These markers will be useful for analyzing the population genetic structure and gene flow of L. erythropterus.
Assuntos
Repetições de Microssatélites/genética , Perciformes/genética , Alelos , Animais , Fluxo Gênico , Variação Genética , Genética Populacional , Biblioteca Genômica , Heterozigoto , Polimorfismo Genético , Análise de Sequência de DNARESUMO
The purpleback flying squid (Sthenoteuthis oualaniensis) is a pelagic squid with tremendous potential for commercial exploitation. We isolated and characterized 21 polymorphic microsatellite loci for S. oualaniensis using a (GT)13-enriched genomic library. The number of alleles per locus varied from 6 to 32. The observed and expected heterozygosities ranged from 0.188 to 0.890, and 0.537 to 0.968, respectively. No significant linkage disequilibrium was detected at these loci. Five loci significantly deviated from the Hardy-Weinberg equilibrium, and four loci may have exhibited null alleles. These microsatellite markers will facilitate further studies in population genetics and the sustainable utilization of S. oualaniensis.
Assuntos
DNA/genética , DNA/isolamento & purificação , Decapodiformes/genética , Loci Gênicos , Repetições de Microssatélites/genética , Animais , Polimorfismo GenéticoRESUMO
We isolated and characterized 22 polymorphic microsatellite loci in Lutjanus erythropterus using a (GT)13-enriched genomic library. We found between 2 and 8 alleles per locus, with a mean of 4.85. The observed and expected heterozygosities ranged from 0.065 to 0.867 and from 0.085 to 0.832, respectively, with means of 0.461 and 0.529, respectively. Allele frequencies in three loci were found to deviate from Hardy-Weinberg equilibrium. Evidence for null alleles was found for three loci. These markers will be useful for distinguishing released captive-bred L. erythropterus individuals from wild individuals.
Assuntos
Loci Gênicos , Repetições de Microssatélites , Perciformes/genética , Polimorfismo Genético , Alelos , Animais , Cruzamento , Pesqueiros , Frequência do Gene , Biblioteca Genômica , HeterozigotoRESUMO
Twenty-two polymorphic microsatellite loci were isolated and characterized from a (GT)13-enriched Nemipterus bathybius genomic library. The number of alleles per locus ranged from 4 to 13, with an average of 7.86. The observed and expected heterozygosity was 0.167-0.889 and 0.278-0.904, respectively, with averages of 0.590 and 0.690. Three loci deviated from Hardy-Weinberg proportions, and 2 loci showed evidence of null alleles. No significant linkage disequilibrium was detected in the pairwise comparisons among the 22 loci. These markers are expected to be useful for the population genetic analysis of N. bathybius.