RESUMO
The diagnosis of eosinophilic pneumonia (EP) is rare and challenging. This condition is frequently misdiagnosed as pulmonary tuberculosis, lymphoma, schistosomiasis, Wegener's granuloma, severe acute respiratory syndrome, or severe community-acquired pneumonia. Herein, we report a case in which computed tomography (CT)-guided percutaneous lung biopsy was used to diagnose EP without alveolar eosinophilia or peripheral eosinophilia. A roundworm identified in the patient's stool confirmed the precise diagnosis to be parasitic EP. This is, to our knowledge, the first reported case of EP confirmed by CT-guided percutaneous lung biopsy. CT-guided percutaneous lung biopsy may represent a new tool for the diagnosis of EP in patients without typical alveolar eosinophilia or peripheral eosinophilia.
Assuntos
Ascaríase/diagnóstico , Ascaríase/tratamento farmacológico , Biópsia Guiada por Imagem/métodos , Eosinofilia Pulmonar/diagnóstico por imagem , Eosinofilia Pulmonar/diagnóstico , Albendazol/uso terapêutico , Animais , Anti-Helmínticos/uso terapêutico , Ascaris lumbricoides/efeitos dos fármacos , Tosse , Dispneia , Fezes/parasitologia , Feminino , Febre , Humanos , Metilprednisolona/uso terapêutico , Pessoa de Meia-Idade , Mialgia , Eosinofilia Pulmonar/parasitologia , Tomografia Computadorizada por Raios XRESUMO
Intercellular adhesion molecule-1 (ICAM-1) is an important factor in the progression of inflammatory responses in vivo. To develop a new anti-inflammatory drug to block the biological activity of ICAM-1, we produced a monoclonal antibody (Ka=4.19×10−8 M) against human ICAM-1. The anti-ICAM-1 single-chain variable antibody fragment (scFv) was expressed at a high level as inclusion bodies in Escherichia coli. We refolded the scFv (Ka=2.35×10−7 M) by ion-exchange chromatography, dialysis, and dilution. The results showed that column chromatography refolding by high-performance Q Sepharose had remarkable advantages over conventional dilution and dialysis methods. Furthermore, the anti-ICAM-1 scFv yield of about 60 mg/L was higher with this method. The purity of the final product was greater than 90%, as shown by denaturing gel electrophoresis. Enzyme-linked immunosorbent assay, cell culture, and animal experiments were used to assess the immunological properties and biological activities of the renatured scFv.
Assuntos
Animais , Feminino , Humanos , Masculino , Camundongos , Expressão Gênica/fisiologia , Fragmentos de Imunoglobulinas/biossíntese , Molécula 1 de Adesão Intercelular/imunologia , Redobramento de Proteína , Renaturação Proteica , Anticorpos de Cadeia Única/biossíntese , Complexo Antígeno-Anticorpo , Anti-Inflamatórios/farmacologia , Anticorpos Monoclonais/biossíntese , Adesão Celular , Cromatografia , Diálise , Ensaio de Imunoadsorção Enzimática , Pavilhão Auricular/efeitos dos fármacos , Escherichia coli/genética , Vetores Genéticos , Fragmentos de Imunoglobulinas/farmacologia , Corpos de Inclusão/metabolismo , Molécula 1 de Adesão Intercelular/efeitos dos fármacos , Leucócitos Mononucleares/metabolismo , Plasmídeos , Engenharia de Proteínas/métodos , Anticorpos de Cadeia Única/farmacologia , Xilenos/farmacologiaRESUMO
Intercellular adhesion molecule-1 (ICAM-1) is an important factor in the progression of inflammatory responses in vivo. To develop a new anti-inflammatory drug to block the biological activity of ICAM-1, we produced a monoclonal antibody (Ka=4.19 × 10(-8) M) against human ICAM-1. The anti-ICAM-1 single-chain variable antibody fragment (scFv) was expressed at a high level as inclusion bodies in Escherichia coli. We refolded the scFv (Ka=2.35 × 10(-7) M) by ion-exchange chromatography, dialysis, and dilution. The results showed that column chromatography refolding by high-performance Q Sepharose had remarkable advantages over conventional dilution and dialysis methods. Furthermore, the anti-ICAM-1 scFv yield of about 60 mg/L was higher with this method. The purity of the final product was greater than 90%, as shown by denaturing gel electrophoresis. Enzyme-linked immunosorbent assay, cell culture, and animal experiments were used to assess the immunological properties and biological activities of the renatured scFv.