RESUMO
PURPOSE: To investigate the effect of microRNA-543 (miR-543) on the proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of triple-negative breast cancer (TNBC) cells, and the associated mechanism. METHODS: Human breast cancer cells (MDA-MB-231, HCC1937, and MCF-7, ZR-75-1) and normal human breast epithelial cell line (MCF10A) were transfected with miR-543 mimics or inhibitor using lipofectamine 2000. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blotting were used to determine the mRNA and protein expression levels of miR-543, actin-like protein 6A (ACTL6A), vimentin, Snail, and E-cadherin in breast cancer cells/tissue. Cell counting kit-8 (CCK-8), wound-healing, and Transwell assays were used to measure the effect of miR-543 on TNBC cell proliferation, invasion, and migration. Overall survival was determined using data from Gene Expression Omnibus (GEO) and Cancer Genome Atlas (TCGA) databases. Bioinformatics analysis and luciferase reporter gene assay were used to determine the regulatory effect of miR-543 on ACTL6A. RESULTS: The level of expression of miR-543 was significantly lower in breast cancer cells/tissue than in normal human breast epithelial cell/tissue (p < 0.05). MicroRNA-543 expression level was significantly reduced in TNBC cells/tissue, relative to the other breast cancer cells/normal breast tissue (p < 0.05). MicroRNA-543 significantly suppressed tumor growth and the proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of TNBC cells, in mouse xenograft model (p < 0.05). CONCLUSIONS: miR-543 influences the biological behavior of TNBC cells by directly targeting ACTL6A gene. miR-543 could serve as a novel diagnostic and therapeutic target for TNBC.
Assuntos
Actinas/fisiologia , Movimento Celular , Proliferação de Células , Proteínas Cromossômicas não Histona/fisiologia , Proteínas de Ligação a DNA/fisiologia , Regulação para Baixo , Transição Epitelial-Mesenquimal , MicroRNAs/fisiologia , Neoplasias de Mama Triplo Negativas/patologia , Animais , Humanos , Camundongos , Invasividade Neoplásica , Células Tumorais CultivadasRESUMO
Tibetan barley is a staple food for the natives of Qinghai-Tibet Plateau. Drought causes a reduction in barley production. In this study, the full-length cDNA of a gene encoding a syntaxin-associated protein was cloned from the leaves of a drought-resistant variety of barley, "Himalaya 10"; its expression was evaluated during drought stress and rehydration via real-time PCR. The cloned HbSYR1 cDNA sequence was 1300 bp in length, and included an 840-bp open reading frame that encoded 279 amino acids. Sequence analysis predicted the molecular weight of the encoded protein to be 42.08 kDa, with an isoelectric point of 4.98. ScanProsite analysis showed that the HbSYR1 protein contained a SNARE family characteristic motif, five casein kinase II phosphorylation sites, two N-glycosylation sites, four protein kinase C phosphorylation sites, and two N-myristoylation sites. The TMHMM prediction program indicated that the protein does not contain a transmembrane transfer ribbon. According to the SignalP 3.0 server, this protein does not contain a signal peptide, and is not a secretory protein. Instead, this protein was suggested to be localized in the cytoplasm, as predicted by the protein subcellular localization prediction tool (PSORT). Our results indicated that HbSYR was induced by drought stress and rehydration, and was determined to be a key gene for drought resistance and water retention in barley.
Assuntos
Hordeum/genética , Proteínas de Plantas/genética , Proteínas Qa-SNARE/genética , Motivos de Aminoácidos , Clonagem Molecular , Secas , Hordeum/fisiologia , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Domínios Proteicos , Proteínas Qa-SNARE/química , Proteínas Qa-SNARE/metabolismo , Estresse FisiológicoRESUMO
Thyroid cancer is the most common type of endocrine tumor. MicroRNAs (miRNAs) play a critical role in a variety of diseases, especially cancer occurrence and progression. However, the specific mechanism by which miRNAs trigger disease states has not been fully elucidated. This study aims to investigate the role of miR-27a in thyroid cancer cells. A wound healing assay was adopted to examine cell migration. A transwell assay was applied to assess cell invasion. A thyroid cancer xenograft model was established using BALB/c nude mice. Western blot was performed to quantify iNOS expression. Tumor tissue blood vessel density was evaluated via immunohistochemistry assays. The results indicated that miR-27a downregulation inhibited thyroid cancer cell migration, while upregulation of miR-27a promoted thyroid cancer cell migration (P < 0.05). Furthermore, reduction in miR-27a expression suppressed thyroid cancer cell invasion (P < 0.05). In the nude mouse model of thyroid cancer xenograft, upregulation of miR-27 induced iNOS expression in pathological tumor tissues, whereas miR-27a inhibition resulted in the opposite effect (P < 0.05). CD105 level was also significantly increased during miR-27a upregulation, and was declined when miR-27a was inhibited (P < 0.05). In conclusion, miR-27a upregulation in thyroid cancer cells affects tumor cell migration, invasion, and angiogenesis by targeting downstream genes. Therefore, miR27a may act as a biomarker of thyroid cancer.
Assuntos
MicroRNAs/genética , Neovascularização Patológica/genética , Neoplasias da Glândula Tireoide/genética , Animais , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Endoglina/genética , Endoglina/metabolismo , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Camundongos , Camundongos Nus , Invasividade Neoplásica , Transplante de Neoplasias , Neovascularização Patológica/metabolismo , Neoplasias da Glândula Tireoide/metabolismoRESUMO
The abscisic acid (ABA) signaling pathway is known as one of the most important signaling pathways in plants and is mediated by multiple regulators. The genes SnRK2, PYR/PYL/RCAR, and ABF are relevant to both ABA-dependent and -independent signaling pathways. To elucidate the profile of these genes from Tibetan hulless barley (Hordeum vulgare L. var. nudum Hook. f.), we collected available sequences from RNA-Seq data, together with NCBI data from five other model plant species (Arabidopsis thaliana, Brachypodium distachyon, Oryza sativa, Populus trichocarpa, and Sorghum bicolor). Gene trees of SnRK2, PYR/PYL/RCAR, and ABF were constructed using a neighbor joining (NJ) method. For all genes, we identified a dominant group in which all six species were represented. Three, four, and five groups were found in the NJ trees of SnRK2, PYR/PYL/RCAR, and ABF, respectively. For each gene, Tibetan hulless barley was divided into three groups. Our analyses indicated that Tibetan hulless barley was associated with B. distachyon. The NJ cluster analysis also suggested that Tibetan hulless barley was affiliated with S. bicolor (SnRK2), A. thaliana (PYR/PYL/RCAR), and O. sativa (ABF). These results illustrate a diverse expression of genes SnRK2, PYR/PYL/RCAR, and ABF, and suggest a relationship among the six species studied. Collectively, our characterization of the three components of the ABA signaling pathway may contribute to improve stress tolerance in Tibetan hulless barley.
Assuntos
Genes de Plantas , Hordeum/genética , Filogenia , Análise por Conglomerados , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismoRESUMO
Type 2 diabetes mellitus is the most common form of endocrine disease in humans; genetic factors are known to contribute to the development of this disease. In this case-control study, we investigated the relationship between the -1082G/A, -819C/T, and -592C/A polymorphisms in interleukin 10 (IL-10) and the pathogenesis of type 2 diabetes mellitus in a Chinese population. Patients with type 2 diabetes mellitus (N = 228) and control subjects (N = 240) were recruited from the Department of Endocrinology at the People's Hospital of Linyi City, between September 2013 and April 2015. The IL-10 -1082G/A, -819C/T, and -592C/A polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism. Multivariate logistic regression analyses revealed that patients carrying the AA genotype of IL-10 -592C/A were at a higher risk of developing type 2 diabetes mellitus compared to those carrying the CC genotype [adjusted odds ratio (OR) = 1.74; 95% confidence interval (CI) = 1.03-2.95]. In addition, individuals carrying the A allele of IL-10 -592C/A showed a 1.34-fold higher risk of developing type 2 diabetes mellitus compared to those carrying the C allele (adjusted OR = 1.34; 95%CI = 1.03- 1.75). There was no significant correlation between the IL-10 -1082G/ A and -819C/T polymorphisms and risk of type 2 diabetes mellitus. In conclusion, this study shows that the -1082G/A polymorphism of IL-10 contributes to the onset of type 2 diabetes mellitus, and may be considered a biomarker for early screening of type 2 diabetes mellitus in the Chinese population studied here.
Assuntos
Diabetes Mellitus Tipo 2/diagnóstico , Diabetes Mellitus Tipo 2/genética , Predisposição Genética para Doença , Interleucina-10/genética , Polimorfismo de Nucleotídeo Único , Adulto , Idade de Início , Alelos , Povo Asiático , Biomarcadores/sangue , Estudos de Casos e Controles , Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/etnologia , Diagnóstico Precoce , Feminino , Expressão Gênica , Humanos , Interleucina-10/sangue , Masculino , Pessoa de Meia-Idade , Razão de Chances , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Regiões Promotoras Genéticas , Fatores de RiscoRESUMO
Hulless barley is an important crop cereal in Tibetan, China. Drought is a major abiotic stress in barley production. In this study, we cloned the drought-related HbSINA4 gene from the variety 'Himalaya 10' and analyzed its expression patterns under different drought and rehydration conditions. The cDNA of HbSINA4 was 1052 bp long, including an open reading frame of 771 bp that encoded a protein of 256 amino acids. The molecular weight of HbSINA4 protein was predicted to be 29.53 kDa and the theoretical pI was 8.32. Bioinformatic analysis showed that the HbSINA4 gene contained a protein kinase domain profile family signature motif, with high similarity to that of Oryza sativa and Brachypodium distachyon. Real-time polymerase chain reaction (PCR) assays revealed that gene expression declined rapidly with increasing drought stress; in contrast, its expression increased after rehydration treatment. Therefore, the HbSINA4 gene responds to the drought stress and plays an important role in barely drought resistance. Furthermore, our results provide information which may be useful in other temperate crop studies and in aiding resistance to drought.
Assuntos
Genes de Plantas/genética , Hordeum/genética , Estresse Fisiológico/genética , Regulação para Cima/genética , Sequência de Aminoácidos , Sequência de Bases , Brachypodium/genética , China , Clonagem Molecular/métodos , DNA Complementar/genética , Secas , Regulação da Expressão Gênica de Plantas/genética , Dados de Sequência Molecular , Proteínas de Plantas/genéticaRESUMO
Phosphatidylinositol-3-OH kinase and RAS-activated signaling pathways play an important role in tumor formation. Abnormalities in relevant genes play essential roles in the occurrence and development of many human cancers. Studies of breast cancer have mainly focused on the women in western countries, but few studies have examined the frequency of mutations in PIK3CA, BRAF, and KRAS in Chinese breast cancer patients. In this study, we conducted sequence analysis of PIK3CA, BRAF, and KRAS and determined relationships with the occurrence of breast cancer in women from Qinghai. DNA was extracted from 25 cases of human breast cancer tissue samples. PIK3CA, BRAF, and KRAS mutation analysis was performed by polymerase chain reaction and DNA sequencing. No mutations were found in PIK3CA, BRAF, and KRAS of adjacent tissues. However, PIK3CA mutations were observed in 32% (8) of the 25 breast cancer tissues examined, in which exon 9 accounted for 4% (1), exon 20 accounted for 28% (7), and no mutations were found in exon 1 of PIK3CA. Sequencing of exon 2 of KRAS suggested that 20% (5) of the 25 samples harbored a mutation and 16% (4) of BRAF harbored a mutation. Any mutation in these 3 oncogenes may induce the occurrence and development of breast cancer.
Assuntos
Neoplasias da Mama/genética , Proteínas de Membrana/genética , Proteínas dos Microfilamentos/genética , Fosfatidilinositol 3-Quinases/genética , Proteínas Proto-Oncogênicas B-raf/genética , Povo Asiático/genética , Neoplasias da Mama/patologia , China , Classe I de Fosfatidilinositol 3-Quinases , Éxons/genética , Feminino , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , MutaçãoRESUMO
The aim of this study was to identify the factors related to and determine the prevalence of Parkinson's disease (PD) among Uygur residents in Hetian Prefecture, Xinjiang Uygur Autonomous Region. This population-based prospective cohort study used structured questionnaires to screen for factors related to PD. The prevalence in different age groups was analyzed, and PD risk was assessed using univariate and multivariate conditional logistic regression analysis. In total, 5932 subjects participated in the survey; of these, 88 individuals, all of whom were Uygurs, had PD. The overall prevalence of PD was 1.48% (1.32%) in people over the age of 45; the prevalence was 1.68% (1.59%) in men and 1.28% (1.36%) in women. The Fifth Population Census in China found the prevalence of PD in Hetian, Xinjiang, to be 1.32%. Single-factor results showed that exercise, social activities, and frequent consumption of nuts are protective factors for PD, and long-term pesticide exposure, family history of PD, and consumption of barbecued food were risk factors for this condition. Multi-factor conditional logistic regression analysis showed that participation in sports and social activities can reduce the risk of PD, whereas long-term pesticide exposure and family history of PD increases this risk. These results show that PD is caused by the interaction of several factors.
Assuntos
Doença de Parkinson/epidemiologia , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Estudos de Coortes , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Estudos Prospectivos , Fatores de Risco , Inquéritos e QuestionáriosRESUMO
Gene polymorphism of vitamin D-binding protein (VDBP) correlates with chronic obstructive pulmonary disease (COPD), but the results remain inconclusive. We aimed to explore the association between VDBP gene polymorphism and COPD. We searched MEDLINE, Embase, Web of Science, and China National Knowledge Infrastructure for publications addressing the association between VDBP gene polymorphism and COPD. After qualitative evaluation, randomized controlled trials were pooled using either a fixed- or a random-effect model depending upon the degree of heterogeneity. Eleven studies with 3144 subjects were included. The genotype group-specific component (GC)*1F-1F was significantly associated with COPD in Asians [odds ratio (OR) = 1.73, 95% confidence interval (CI) = 1.07-2.81, P = 0.03], but not in Caucasians (OR = 1.44, 95%CI = 0.57-3.66, P = 0.45). A protective effect of GC*1F-1S was observed in Asians (OR = 0.70, 95%CI = 0.55-0.89, P = 0.003) but not in Caucasians (OR = 0.93, 95%CI = 0.69-1.24, P = 0.61). There was no association of GC*1S-1S, GC*2-1S and GC*1F-2 with COPD. As for alleles, GC*1F was a risk factor, whereas GC*1S was protective against COPD in Asians; GC*2 was not protective. The genotype GC*1F-1F or allele GC*1F was associated with increased susceptibility to COPD in Asians. No protective effect of genotype GC*2-2 against COPD was found. The protective effects of GC*1F-1S and GC*1S were observed in Asians but not in Caucasians. The VDBP gene polymorphism could be a potential marker for screening of COPD.
Assuntos
Predisposição Genética para Doença , Polimorfismo Genético , Doença Pulmonar Obstrutiva Crônica/etnologia , Doença Pulmonar Obstrutiva Crônica/genética , Proteína de Ligação a Vitamina D/genética , Alelos , Povo Asiático , Expressão Gênica , Frequência do Gene , Genótipo , Humanos , Modelos Genéticos , Razão de Chances , Doença Pulmonar Obstrutiva Crônica/patologia , Fatores de Risco , População BrancaRESUMO
We investigated the effect of flavonoid compounds extracted from species of genus Iris L. on carbon tetrachloride (CCl4)-induced rat liver fibrosis. Thirty Sprague-Dawley rats were randomly divided into normal control group, liver fibrosis model group, and drug treatment group (N = 10 each). Next, 0.2 mL/100 g CCl4 was subcutaneously injected for 6 weeks in both model and treatment rats to generate the liver fibrosis model. In the control group, an equal volume of castor oil was injected subcutaneously. Rats in the treatment group also received 100 mg·kg(-1)·day(-1) flavonoid compounds via gastric tubes. After 6 weeks, rats were sacrificed, and their liver tissues were examined for pathological changes, including alanine aminotransferase, aspartate aminotransferase, total bilirubin, hyaluronic acid, laminin, and procollagen type-3. Liver tissues from control rats showed no significant pathological changes, while model animals showed significant liver fibrosis. In the treatment group, liver fibrosis significantly decreased compared to the model group (P < 0.05). Liver fibrotic indices, including hyaluronic acid, laminin, and procollagen type-3, in treatment rats were all significantly lower than those in the model group (P < 0.05), but not significantly different compared to the normal group (P > 0.05). Other liver function indices, including alanine aminotransferase, aspartate aminotransferase, and total bilirubin, in treatment rats were also significantly lower than those in model rats (P < 0.01) but higher than those in control animals (P < 0.05). Flavonoid compounds extracted from Iris plants showed significant inhibitory effects on CCl4-induced rat liver fibrosis.
Assuntos
Flavonoides/farmacologia , Gênero Iris/química , Cirrose Hepática/prevenção & controle , Animais , Tetracloreto de Carbono/toxicidade , Modelos Animais de Doenças , Flavonoides/isolamento & purificação , Fígado/efeitos dos fármacos , Fígado/patologia , Cirrose Hepática/induzido quimicamente , Testes de Função Hepática , Masculino , Distribuição Aleatória , Ratos , Ratos Sprague-DawleyRESUMO
DDX6 belongs to a family of DEAD-box RNA helicases, which are RNA splicing proteins that ensure the correct folding and structure of mature RNA. Gametogenesis requires the participation of many kinds of RNA. To explore its functions during Eriocheir sinensis gametogenesis, we cloned a full-length DDX6 cDNA sequence from E. sinensis (Es-DDX6) which contains a 1536-nucleotide open reading frame encoding a 512-amino acid protein. Multiple sequence alignments showed that Es-DDX6 has ten conservative DEAD-box family motifs. Tissue expression analysis of Es-DDX6 mRNA and protein levels showed that Es-DDX6 was highly expressed in both the ovary and testis. qRT-PCR analysis revealed the widespread expression of Es-DDX6 mRNA during various stages of gonad development peaking in October. In addition, immunohistochemical studies showed that oocytes and the spermatogonium and primary spermatocytes of testes contained high levels of cytoplasmic Es-DDX6 and decreased expression levels in spermatids. Interestingly, there was no expression of Es-DDX6 in these cells as they matured along the male reproductive system. Since oocytes and spermatocytes are active in meiosis and oocytes undergo rapid growth in October, these results provide preliminary evidence that Es-DDX6 plays a role in E. sinensis gametogenesis and oocyte growth processes.
Assuntos
Braquiúros/genética , RNA Helicases DEAD-box/genética , Gametogênese , Sequência de Aminoácidos , Animais , Braquiúros/metabolismo , Clonagem Molecular , RNA Helicases DEAD-box/metabolismo , Evolução Molecular , Feminino , Expressão Gênica , Perfilação da Expressão Gênica , Masculino , Dados de Sequência Molecular , Especificidade de Órgãos , Ovário/metabolismo , Alinhamento de Sequência , Testículo/metabolismoRESUMO
This study compared the abnormal corneal epithelium after laser-assisted subepithelial keratectomy (LASEK) with dystrophic cornea using in vivo using confocal microscopy (IVCM) and examined the effects of the abnormal epithelium on postoperative recovery of uncorrected distance visual acuity (UDVA) and sub-basal nerve plexus regeneration. The UDVA and wound healing were examined in 50 eyes of 25 patients undergoing LASEK at 1 week, 1 month, and 1 year postoperatively, including the visual acuity, slit lamp microscopy, and IVCM. After 1 week, the corneal epithelium was healed in all patients, but abnormal epithelial cells were detected in 33/50 patients using IVCM. Abnormal cells were limited to the surgical margin, and highly reflective granules were observed underneath. At 1 month and 1 year postoperatively, the abnormal epithelium was unchanged in size. At 1 year postoperatively, there were clear differences between the sub-basal nerve plexus in the normal and abnormal epithelium. At 1 month postoperatively, the UDVA was >1.0 in 88% of patients, which increased to 94% at 1 year, and there was no clear difference in the UDVA between abnormal (N = 33) and normal (N = 17) epithelium. After LASEK, abnormal epithelial cells may arise at the margin of the epithelial flap and persist 1 year postoperatively accompanied by poor regeneration of the sub-basal nerve plexus. However, this does not affect the UDVA postoperatively. The abnormal epithelium may be caused by residual necrotic basal cell debris on the epithelial basement membrane and abnormal neurotrophic metabolism between the corneal epithelium and nerve plexus.
Assuntos
Ceratectomia Subepitelial Assistida por Laser/métodos , Adulto , Feminino , Humanos , Masculino , Microscopia Confocal , Miopia/fisiopatologia , Miopia/cirurgia , Acuidade Visual , Adulto JovemRESUMO
The aim of this study was to investigate the effects of the vascular endothelial growth factor (VEGF)/VEGF receptor (VEGFR)/K-ras signaling pathways on miRNA21 levels in hepatocellular carcinoma tissues in rats. Eighteen male Sprague-Dawley rats were randomly divided into normal control, model, and VEGF blocking agent groups (N = 6/group). The expression of VEGF mRNA, K-ras protein, and miRNA21 increased significantly (P < 0.05) in the model group compared with the normal control group, and decreased dramatically in the VEGF blocking agent group compared to the model group. The expression of VEGFR mRNA in the model group was higher than that of the control group, and the expression of VEGFR mRNA in the VEGF blocking agent group was significantly higher than that of the control group (P < 0.05). Statistically, there was no difference between the expression of VEGFR mRNA for the VEGF blocking agent group and the model group (P > 0.05). Finally, the expression of the miRNA21 gene in the VEGF blocking agent group was higher than in the control group, and there was a significant statistical difference noted; Pearson's correlation analysis demonstrated that the expression of K-ras protein was positively correlated with miRNA21 in the experimental groups (P = 0.001). The above results showed that the VEGF/VEGFR/K-ras signaling pathway might promote the occurrence and development of hepatocellular carcinoma cells through regulating expression of miRNA21, which has potential clinical value for the development of therapies against biological targets and determining prognosis for patients with hepatocellular carcinoma.
Assuntos
Carcinoma Hepatocelular/genética , Neoplasias Hepáticas/genética , MicroRNAs/biossíntese , Proteínas Proto-Oncogênicas p21(ras)/biossíntese , Fator A de Crescimento do Endotélio Vascular/biossíntese , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/biossíntese , Animais , Carcinoma Hepatocelular/induzido quimicamente , Carcinoma Hepatocelular/patologia , Dietilnitrosamina/toxicidade , Modelos Animais de Doenças , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Hepáticas/induzido quimicamente , Neoplasias Hepáticas/patologia , MicroRNAs/genética , Neovascularização Patológica/genética , Neovascularização Patológica/patologia , Proteínas Proto-Oncogênicas p21(ras)/genética , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Ratos , Transdução de Sinais/efeitos dos fármacos , Fator A de Crescimento do Endotélio Vascular/antagonistas & inibidores , Fator A de Crescimento do Endotélio Vascular/genética , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/genética , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/genéticaRESUMO
We performed a study to investigate the role of ERCC1 (rs11615, rs2298881, and rs3212986) and ERCC2 (rs13181, rs238406, and rs1799793) polymorphisms in the prognosis of gastric cancer. A total of 346 patients with gastric cancer were recruited between May 2009 and May 2012. Single nucleotide polymorphism genotyping was performed using the Sequenom MassARRAY platform. The GA+AA genotype of ERCC2 rs1799793 showed significant and favorable response to chemotherapy than the wide-type GG genotype in multivariate analysis (OR = 1.78, 95%CI = 1.13-2.81). In a Cox proportional hazard model, carriers of ERCC2 rs1799793 GA+AA genotype exhibited longer duration of survival than did those with the GG genotype (hazards ratio = 0.57, 95%CI = 0.35-0.92). In conclusion, our study suggests that ERCC2 rs1799793 polymorphic variation could be used as a predictor for the prognosis of gastric cancer.
Assuntos
Proteínas de Ligação a DNA/genética , Endonucleases/genética , Prognóstico , Neoplasias Gástricas/genética , Proteína Grupo D do Xeroderma Pigmentoso/genética , Idoso , Biomarcadores Farmacológicos , Reparo do DNA/genética , Feminino , Estudos de Associação Genética , Genótipo , Humanos , Estimativa de Kaplan-Meier , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único , Neoplasias Gástricas/tratamento farmacológico , Neoplasias Gástricas/patologia , Resultado do TratamentoRESUMO
Serum high-sensitivity C-reactive protein (hs-CRP) is a sensitive indicator of inflammation, which is closely related with the progress of plaque formation. Interleukin-6 (IL-6) is one of the inflammatory markers of local coronary plaque and the peripheral blood cycle, promoting the occurrence of atherosclerosis development and plaque rupture. In this study, the correlation of hs-CRP and IL-6 was investigated in patients with acute coronary syndrome (ACS). Sixty cases of ACS, including 33 cases of acute myocardial infarction (AMI) and 27 cases of unstable angina pectoris (UAP), 45 cases of stable angina pectoris (SAP), and 45 healthy people (HG) were enrolled in study. The serum hs-CRP and serum IL-6 levels were tested by the immune turbidimetric method and enzyme-linked immunosorbent assay (ELISA), respectively. The differences among groups and their correlations were evaluated. Results showed that the serum hs-CRP and IL-6 concentrations of the AMI and UAP groups were significantly higher than those of the SAP and HG groups, respectively (P<0.01), and those of the AMI group were significantly higher than those of the UAP group (P<0.05). The serum hs-CRP and IL-6 levels of the ACS group were positively correlated (r=0.836). The serum hs-CRP and IL-6 levels could be used to determine the stability of plaque, and have some relevance in the ACS process, showing great value in judgments of ACS prognosis.
Assuntos
Síndrome Coronariana Aguda/sangue , Síndrome Coronariana Aguda/patologia , Proteína C-Reativa/análise , Interleucina-6/sangue , Síndrome Coronariana Aguda/imunologia , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Placa Aterosclerótica/sangue , PrognósticoRESUMO
The effect of different lighting environments on the vision and refractive error were investigated in 427 Kazakhs (828 eyes) aged from 40 to 60 years old, of which 279 were pastoral (546 eyes) and 148 were urban (282 eyes). Pastoral Kazakhs use natural illumination, whereas city Kazakhs mainly use artificial illumination. Of all cases, 19.1% (54 cases) had a vision of 0.4 to 0.5, 20.9% (59 cases) had 0.6 to 0.8, and 17.7% (50 cases) had above 1.0. However, the visual acuity distribution of the pastoral Kazakhs had a more obvious characteristic, which was mainly concentrated above 0.6. The vision of 25.1% (137) of cases was 0.6 to 0.8, whereas 58.4% (319) of cases had vision above 1.0. There were more cases with vision above 1.0 in the pastoral Kazakhs compared to the city Kazakhs (P < 0.05). The diopter of the city Kazakhs was between 0 D and -4.0 D, whereas that of the pastoral Kazakhs was between +2.0 D and -2.0 D. Diopters between +2.0 D and 0 D or less than 3.0 D differed significantly between the two groups. Daylight lamps were the main illumination photosources of the city Kazakhs. Artificial light illuminated 70.9% (105 cases) of the city Kazakhs for more than 6 h. By contrast, natural light illuminated 75.3% (210 cases) of the pastoral Kazakhs for an outdoor activity time of more than 9 h. These results suggest that poor lighting environment is a very important factor contributing to refractive error.
Assuntos
Meio Ambiente , Iluminação , Visão Ocular/fisiologia , Acuidade Visual , Adulto , Etnicidade , Feminino , Humanos , Cazaquistão , Masculino , Pessoa de Meia-Idade , Erros de RefraçãoRESUMO
Curcumin has been widely used for the prevention and treatment of Alzheimer's disease (AD), but its mechanism is still not clear. Inhibitory factors of axonal regeneration have been shown to cause a series of pathophysiological changes in the early period of AD. In this study, the co-receptor (Nogo receptor; NgR) of three axonal growth-inhibitory proteins was examined, and effects of curcumin on spatial learning and memory abilities and hippocampal axonal growth were investigated in amyloid ß-protein (Aß)1-40-induced AD rats. Results showed that the expression of NgR in the AD group significantly increased and the number of axonal protein-positive fibers significantly reduced. The spatial learning and memory abilities of AD rats were significantly improved in the curcumin group. Furthermore, hippocampal expressions of NgR mRNA and protein decreased, and the expression of axonal protein significantly increased. There was a negative correlation between the expression of NgR and axonal growth. Together, these results suggested that curcumin could improve the spatial learning and memory abilities of AD rats. The mechanism might be related with its lowering of hippocampal NgR expression and promoting axonal regeneration.
Assuntos
Doença de Alzheimer/metabolismo , Axônios/metabolismo , Curcumina/farmacologia , Hipocampo/efeitos dos fármacos , Hipocampo/metabolismo , Proteínas da Mielina/metabolismo , Receptores de Superfície Celular/metabolismo , Doença de Alzheimer/tratamento farmacológico , Doença de Alzheimer/genética , Doença de Alzheimer/fisiopatologia , Peptídeos beta-Amiloides/efeitos adversos , Animais , Axônios/patologia , Curcumina/administração & dosagem , Modelos Animais de Doenças , Proteínas Ligadas por GPI/genética , Proteínas Ligadas por GPI/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Hipocampo/patologia , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Proteínas da Mielina/genética , Receptor Nogo 1 , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos , Receptores de Superfície Celular/genéticaRESUMO
The porcine reproductive and respiratory syndrome virus (PRRSV) continues to be a threat, exerting significant economic effects on the swine industry worldwide. However, none of the current commercially available vaccines can completely prevent respiratory infection, trans-placental transmission, pig-to-pig transmission of the virus, or maintain immune protection in sows. This study provides information on PRRSV and a review of available options for PRRS control strategies based on its pathogenic characteristics, immune properties, and biological characteristics. In this study, the nisin-controlled expression system of Lactococcus lactis was selected as a vector to express the ORF6 gene of PRRSV. Food-grade recombinant, L. lactis PNZ8149/NZ3900-M/PRRS, which contained the lactose operon, was successfully constructed. The molecular weight of the expressed recombinant protein was approximately 19 kDa. Furthermore, the recombinant protein was located on the surface of L. lactis and showed reactogenicity with the antibody against PRRSV. Results of this study are expected to lay a theoretical foundation for development of genetically engineered L. lactis mucosal vaccines and to provide information related to its immune activity and adjuvant effects.
Assuntos
Expressão Gênica/efeitos dos fármacos , Genes Virais , Lactococcus lactis/genética , Nisina/farmacologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/genética , Animais , Linhagem Celular , Vetores Genéticos/genética , Vetores Genéticos/metabolismo , Lactococcus lactis/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Suínos/virologiaRESUMO
Apnea and the respiratory cycle are dynamic processes in obstructive sleep apnea-hypopnea (OSAH), which occur only during sleep. Our study aimed to observe the dynamic changes in the soft palate and the uvula during wakefulness and sleep using ultrafast magnetic resonance imaging (UMRI) to provide reference data for the pathogenesis and treatment of OSAH. The dynamic changes in the soft palate and uvular tip of 15 male patients (average age: 50.43 ± 9.82 years) with OSAH were evaluated using UMRI of the upper airway while asleep and awake after 1 night of sleep deprivation. A series of midline sagittal images of the upper airway were obtained. The distance from the center of the soft palate to the x-axis (an extended line from the anterior nasal spine to the posterior nasal spine), from the uvular tip to the x-axis, from the center of the soft palate to the y-axis (a perpendicular line from the center of the pituitary to the x-axis), and from the uvular tip to the y-axis (designated as PX, UX, PY, and UY, respectively) were measured during sleep and wakefulness. The minimum PX, PY, UX, and UY were shorter during sleep than during wakefulness, whereas the maxima were longer during sleep (P < 0.01), the differences between the maximum and minimum PX, PY, UX, and UY were larger during sleep (P < 0.01). The upward, downward, forward, and backward ranges of movement of the soft palate and the uvular tip were larger during sleep in OSAH patients. This increased compliance may trigger each airway obstructive event.
Assuntos
Apneia Obstrutiva do Sono/fisiopatologia , Úvula/fisiopatologia , Adulto , Humanos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Movimento , Apneia Obstrutiva do Sono/diagnósticoRESUMO
ß-arrestins are expressed proteins that were first described, and are well-known, as negative regulators of G protein-coupled receptor signaling. Penehyclidine hydrochloride (PHC) is a new anti-cholinergic drug that can inhibit biomembrane lipid peroxidation, and decrease cytokines and oxyradicals. However, to date, no reports on the effects of PHC on ß-arrestin-1 in cells have been published. The aim of this study was to investigate the effect of PHC on ß-arrestin-1 expression in lipopolysaccharide (LPS)-induced human pulmonary microvascular endothelial cells (HPMEC). Cultured HPMEC were pretreated with PHC, followed by LPS treatment. Muscarinic receptor mRNAs were assayed by real-time quantitative PCR. Cell viability was assayed by the methyl thiazolyl tetrazolium (MTT) conversion test. The dose and time effects of PHC on ß-arrestin-1 expression in LPS-induced HPMEC were determined by Western blot analysis. Cell malondialdehyde (MDA) level and superoxide dismutase (SOD) activity were measured. It was found that the M3 receptor was the one most highly expressed, and was activated 5 min after LPS challenge. Furthermore, 2 µg/mL PHC significantly upregulated expression of ß-arrestin-1 within 10 to 15 min. Compared with the control group, MDA levels in cells were remarkably increased and SOD activities were significantly decreased in LPS pretreated cells, while PHC markedly decreased MDA levels and increased SOD activities. We conclude that PHC attenuated ROS injury by upregulating ß-arrestin-1 expression, thereby implicating a mechanism by which PHC may exert its protective effects against LPS-induced pulmonary microvascular endothelial cell injury.