RESUMO

The mass profiles of cell-free extracts of 180 commensal and pathogenic strains of Escherichia coli were determined by MALDI-TOF mass spectrometry (MS). While some peaks were highly conserved in all E. coli, several peaks occurred only in some strains, showing heterogeneity among them. We did not detect strain-specific peaks for any of the E. coli categories tested. However, review of the fully conserved and the variable peaks suggested that MALDI-TOF MS has the potential to distinguish commensal and uropathogenic E. coli strains. Additionally, eight Shigella sonnei isolates were tested and found to be indistinguishable from E. coli by MALDI-TOF MS under the test conditions.

Assuntos

Sistema Livre de Células , Escherichia coli/química , Shigella sonnei/classificação , Escherichia coli/classificação , Escherichia coli/patogenicidade , Humanos , Shigella sonnei/química , Shigella sonnei/patogenicidade , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz