RESUMO
A new serogroup of Vibrio cholerae non-O1, O:139, has been implicated in recent epidemics. It was scanned with a factor A-specific fluoresceine-conjugated monoclonal antibody, searching for antigen determinants by laser flow cytometry. First, a group of gram-negative 4-amine-4, 6 dideoxy-D-mannose antigen-related microorganisms were tested to assess monoclonal antibody cross reactions. Later, a clear recognition of antigen determinants was found with this monoclonal antibody, on V. cholerae non-O1, O:136, Bengal, and MO45 strains, showing no cross reactions with the antigenically related non O1, O:22, and Inaba and Ogawa O1 strains. On the other hand, factor A of V. cholerae O1 strains was recognized by the specific monoclonal antibody and a discrete factor A on V. cholerae non-O1, O:139, Bengal and MO45 strains was detected.
Assuntos
4-Butirolactona/análogos & derivados , Citometria de Fluxo/métodos , Substâncias de Crescimento/análise , Vibrio cholerae/classificação , 4-Butirolactona/análise , 4-Butirolactona/biossíntese , Substâncias de Crescimento/biossíntese , Humanos , Lasers , Sorotipagem , Vibrio cholerae/metabolismoRESUMO
Antibodies against Vibrio cholerae were determined in 2352 serum samples obtained from patients with clinical diagnosis of cholera. Samples from their contacts and from healthy people living in the same communities were also analyzed. Vibriocidal antibodies with titers 1:160 or higher were observed in 25% of the samples. An increase of vibriocidal and antitoxin antibody titers were observed in 56 to 60% of the patients in which paired samples were available, one obtained in the acute phase of the disease and the other in the convalescence, confirming the diagnosis of cholera. Differences in the antibody titers were noticed when comparing the serotype according to the geographic area and the season of the year.
Assuntos
Cólera/epidemiologia , Doença Aguda , Anticorpos Antibacterianos/sangue , Cólera/microbiologia , Convalescença , Surtos de Doenças , Humanos , México/epidemiologia , Prevalência , Estações do Ano , Estudos Soroepidemiológicos , Sorotipagem , Vibrio cholerae/classificação , Vibrio cholerae/imunologiaRESUMO
ELISA test was evaluated in 503 cultures of Vibrio cholerae O1 y 303 Non-O1. The cultures were isolated from sewage from different states of México between june 1991 and october 1992. The sensitivity was 100% and specificity was 96%. Only 12 strains of V. cholerae Non-O1 were positive for CT toxin. When these cultures were confirmed by polymerase chain reaction (PCR) for cholera toxin, the results were negative. ELISA test is a good alternative to be used for toxin production in cultures of V. cholerae, it needs confirmation only with O1 negative and Non-O1 positive reactions.
Assuntos
Toxina da Cólera/análise , Ensaio de Imunoadsorção Enzimática , Esgotos , Vibrio cholerae/metabolismo , Animais , Cólera/epidemiologia , Toxina da Cólera/imunologia , Meios de Cultivo Condicionados/química , Surtos de Doenças , Estudos de Avaliação como Assunto , Cabras/imunologia , Humanos , Soros Imunes , México/epidemiologia , Reação em Cadeia da Polimerase , Sensibilidade e Especificidade , Sorotipagem , Vibrio cholerae/classificação , Vibrio cholerae/imunologia , Vibrio cholerae/isolamento & purificaçãoRESUMO
This study was realized in Minatitlán, Veracruz during a cholera outbreak. 169 rectal swabs were taken from hilles and their contacts. They were transfer alkaline peptone water for enrichment to V. cholerae and incubated for 8 hrs to 37 degrees C, 70 were positive for V. cholerae in both techniques. The coagglutination was done with a reagent prepared at the Instituto de Diagnóstico y Referencia Epidemiológicos of México and the culture were also performed in the same Institute. We obtained 100% of sensitivity and specificity of co-agglutination in relation with culture. This results gave the possibility to use this kind or reagents for a rapid presumptive diagnosis of cholera.