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In regions where water is a limited resource, lettuce production can be challenging. To address this, water management strategies like deficit irrigation are used to improve water-use efficiency in agriculture. Associating this strategy with silicon (Si) application could help maintain adequate levels of agricultural production even with limited water availability. Two lettuce crop cycles were conducted in a completely randomized design, with a factorial scheme (2 × 3), with three irrigation levels (60%, 80% and 100%) of crop evapotranspiration (ETc), and with and without Si application. To explore their combined effects, morphological, productive, physiological and nutritional parameters were evaluated in the crops. The results showed that deficit irrigation and Si application had a positive interaction: lettuce yield of the treatment with 80% ETc + Si was statistically similar to 100% ETc without Si in the first cycle, and the treatment with 60% ETc + Si was similar to 100% ETc without Si in the second cycle. Photosynthetic rate, stomatal conductance, intercellular CO2 concentration, transpiration rate and total chlorophyll content increased under water-stress conditions with Si application; in the first cycle, the treatment with 80% ETc + Si increased by 30.1%, 31.3%, 7.8%, 28.46% and 50.3% compared to the same treatment without Si, respectively. Si application in conditions of water deficit was also beneficial to obtain a cooler canopy temperature and leaves with higher relative water content. In conclusion, we found that Si applications attenuate water deficit effects and provide a strategy to ameliorate the yield and water productivity in lettuce crops, contributing to more sustainable practices in agriculture.

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Objetivo: Identificar las causas de exclusión de los donantes renales vivos en un centro hospitalario de tercer nivel en el noroeste de México. Materiales y métodos: Estudio de tipo observacional, transversal, descriptivo, retrospectivo. Se realizó la evaluación de los expedientes clínicos de los candidatos vivos para donación renal, que abarcó el periodo comprendido entre el 1 de enero de 2019 y el 31 de diciembre de 2021. Resultados: De los 30 expedientes seleccionados, solo se eligieron 2 (6,6 %) donadores, es decir, existió un índice de rechazo de 28 (93,3 %) de los potenciales candidatos. La edad promedio fue de 40,7 años; al dividirlos en grupos de edad, se observó que el 7,44 % fueron ≤39 años; el 5,31 %, ≥50 años; y el 4,25 %, de 40 a 49 años. Dentro de las principales patologías que originaron el rechazo del donador vivo se encuentran las enfermedades crónicas, como afección renal desconocida, obesidad, diabetes mellitus, hipertensión arterial sistémica y cardiopatías, que representaron el 60,7 %. El deseo de donar es más frecuente entre consanguíneos que en no consanguíneos. En el caso de los consanguíneos, es decir, hermanos, padres, hijos, tíos, etc., el 64,2 % fue rechazado; en los no consanguíneos, el 34,7 %, que incluía a esposos y amigos. Conclusiones: Los resultados obtenidos sobre el índice de masa corporal (IMC) mostraron que el sobrepeso y la obesidad fueron las principales causas de exclusión, circunstancia que es particular en nuestro país respecto a su perfil epidemiológico. La cantidad de donantes vivos en nuestro centro se redujo porque la mayor parte de la población no es sana, padece obesidad y ello repercute al momento de presentarse como candidato a donante.

Objective: To identify the causes for exclusion of living kidney donors at a third-level hospital in northwestern Mexico. Materials and methods: An observational, cross-sectional, descriptive and retrospective study, in which the medical records of candidates for living kidney donation were evaluated from January 1, 2019 to December 31, 2021. Results: Out of the 30 selected records, only 6.6 % (2) were chosen as donors, i.e., the rejection rate of the potential candidates was 93.3 % (28). The average age was 40.7 years; when divided into age groups, it was observed that 7.44 % were ≤ 39 years, 5.31 % were ≥ 50 years and 4.25 % were in the 40-49 age range. The main pathologies that caused the rejection of living donors were chronic diseases such as unknown renal disease, obesity, diabetes mellitus, systemic hypertension and heart diseases, which accounted for 60.7 %. The desire to donate was more frequent among blood relatives than non-blood relatives. In the case of blood relatives, i.e., siblings, parents, children, uncles, aunts, etc., 64.2 % were rejected, while 34.7 % of non-blood relatives, including spouses and friends, were rejected. Conclusions: Based on the body mass index (BMI), the results showed that overweight and obesity were the main causes of exclusion, a situation that is consistent with the Mexican epidemiological profile. The number of living donors at our center has been reduced because most of the population is not healthy and has obesity, which affects the willingness to be a candidate for living kidney donation.

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The quality of cotton fiber is a fundamental criterion for determining the commercial value of the product, being influenced by the conditions of cultivation. The study aimed to evaluate irrigated and non-irrigated cultivation systems and the management of silicon fertilization on cotton fiber characteristics. The experiment was carried out in the field in Selvíria-MS, in the 2017/2018 agricultural crop. It was adopted a randomized block design, in a 2x6 factorial scheme, with two cultivationconditions (irrigated and rainfed) and six doses of Si (0, 50, 100, 150, 200 and 400 g ha-1), with four replications. Seeds of the genotype TMG 11 WS were used. Irrigation was carried out with sprinklers spaced at 6x6 m. The application of Si via leaf was carried out at 50 and 70 days after emergence. From thecotton fiber, the characteristics of length, micronaire, strength, uniformity, elongation, reflectance, yellowing, degree of leaves, impurity and impurity particles were analyzed. The data submitted to analysis of variance by the F test, the means compared for the cultivation condition, and regression analysis for the amount of Si. The cotton fiber in irrigated cultivation showed improvement in the characteristics resistance, reflectance and yellowing.The application of Si via leaf reduced the leaf grade in the cotton fibers.

A qualidade da fibra doalgodão é critério fundamental para determinação do valor comercial do produto, sendo influenciado pelas condições de cultivo. O estudo teve como objetivo avaliarsistemas de cultivo irrigado e não irrigado e o manejo da adubação de silício sobre características da fibra do algodão. O experimento foi desenvolvido a campo no município de Selvíria-MS, na safra 2017/2018. Foi adotado delineamento em blocos ao acaso, em esquema fatorial 2x6, sendo duas condições de cultivo (irrigado e sequeiro) e seis doses de Si (0, 50, 100, 150, 200 e 400 g ha-1), com quatro repetições. Foi utilizado sementes do genótipo TMG 11 WS. A irrigação foi realizada com aspersores espaçados em 6x6 m. A aplicação de Si via foliar foi realizado aos 50 e 70 dias após a emergência. A partir da fibra do algodão, foram analisadas as características de comprimento, micronaire, resistência, uniformidade, alongamento, reflectância, amarelecimento, graus de folhas, impureza e partículas de impureza.Os dados submetidos a análise de variância pelo teste F, as médias comparadas para a condição de cultivo, e análise de regressão para quantidade de Si. A fibra de algodão em cultivo irrigado apresentou melhoria nas características de resistência, reflectância e amarelamento. A aplicação de Si via foliar reduziu o grau de folhas nas fibras de algodão.

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The quality of cotton fiber is a fundamental criterion for determining the commercial value of the product, being influenced by the conditions of cultivation. The study aimed to evaluate irrigated and non-irrigated cultivation systems and the management of silicon fertilization on cotton fiber characteristics. The experiment was carried out in the field in Selvíria-MS, in the 2017/2018 agricultural crop. It was adopted a randomized block design, in a 2x6 factorial scheme, with two cultivationconditions (irrigated and rainfed) and six doses of Si (0, 50, 100, 150, 200 and 400 g ha-1), with four replications. Seeds of the genotype TMG 11 WS were used. Irrigation was carried out with sprinklers spaced at 6x6 m. The application of Si via leaf was carried out at 50 and 70 days after emergence. From thecotton fiber, the characteristics of length, micronaire, strength, uniformity, elongation, reflectance, yellowing, degree of leaves, impurity and impurity particles were analyzed. The data submitted to analysis of variance by the F test, the means compared for the cultivation condition, and regression analysis for the amount of Si. The cotton fiber in irrigated cultivation showed improvement in the characteristics resistance, reflectance and yellowing.The application of Si via leaf reduced the leaf grade in the cotton fibers.(AU)

A qualidade da fibra doalgodão é critério fundamental para determinação do valor comercial do produto, sendo influenciado pelas condições de cultivo. O estudo teve como objetivo avaliarsistemas de cultivo irrigado e não irrigado e o manejo da adubação de silício sobre características da fibra do algodão. O experimento foi desenvolvido a campo no município de Selvíria-MS, na safra 2017/2018. Foi adotado delineamento em blocos ao acaso, em esquema fatorial 2x6, sendo duas condições de cultivo (irrigado e sequeiro) e seis doses de Si (0, 50, 100, 150, 200 e 400 g ha-1), com quatro repetições. Foi utilizado sementes do genótipo TMG 11 WS. A irrigação foi realizada com aspersores espaçados em 6x6 m. A aplicação de Si via foliar foi realizado aos 50 e 70 dias após a emergência. A partir da fibra do algodão, foram analisadas as características de comprimento, micronaire, resistência, uniformidade, alongamento, reflectância, amarelecimento, graus de folhas, impureza e partículas de impureza.Os dados submetidos a análise de variância pelo teste F, as médias comparadas para a condição de cultivo, e análise de regressão para quantidade de Si. A fibra de algodão em cultivo irrigado apresentou melhoria nas características de resistência, reflectância e amarelamento. A aplicação de Si via foliar reduziu o grau de folhas nas fibras de algodão.(AU)

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Introdução: A pandemia de coronavírus (COVID-19) impactou não apenas a saúde física, em decorrência da síndrome respiratória aguda grave causada pelo vírus, mas também impactou a saúde mental, as áreas econômica e a social. Além disso, o foco do cenário pandêmico foi a contingência do COVID-19, o que propiciou o aparecimento ou o agravamento de outras doenças. Dessa forma, os escopos originados da pandemia tornaram-se potenciais desencadeantes de quadros de estresse e de interferência na qualidade de vida da população. Proposição: avaliar o índice de estresse percebido e a qualidade de vida da população em dois grupos de pessoas: primeiro, as que tiveram diagnóstico confirmado de COVID-19 e, segundo indivíduos que não contraíram a doença. Materiais e Métodos: Participaram da pesquisa 66 indivíduos, entre 18 e 60 anos, sendo 33 de pessoas sem contaminação e 33 pessoas que tiveram diagnóstico confirmado. Os participantes responderam dois questionários: O questionário de estresse percebido (QEP), para avaliar o estresse e outro questionário para avaliação da qualidade de vida (SF-36). Resultados: A análise mostrou que o QEP dos indivíduos não contaminados foi menor (0,64 + 0,04); em comparação aos indivíduos que tiveram o resultado positivo para doença (0,73 + 0,02) (p = 0,0484), diferença estatisticamente significativa, o que mostra que os casos positivos têm percepção que estão mais estressados. Na análise do SF-36, apenas o domínio Capacidade Funcional apresentou diferença estatisticamente significativa, sendo que os voluntários que não tiveram a doença apresentaram um maior valor (93,3 + 2,0), que os contaminados (73,0 + 3,6) (p < 0,0001), o que sugere melhor qualidade de vida aos primeiros. Conclusão: O estudo apresenta resultados que indicam que a pandemia impactou negativamente a saúde da população, pois os participantes diagnosticados com COVID-19 tiveram maior estresse percebido e menor qualidade de vida, em comparação aos não contaminados.

Introduction: The coronavirus pandemic (COVID-19) has not only impacted physical health, as result of the severe acute respiratory syndrome caused by the virus, but has also impacted mental health, the economic and social areas. In addition, the focus of pandemic scenario was the COVID-19 contingency, which led to the appearance or worsening of other diseases. Besides the scopes originated from the pandemic became potential triggers of stress and interference in the population's quality of life. Proposition: to evaluate the perceived stress index and the population's quality of life in two groups of people: first, those who had a confirmed diagnosis of COVID-19 and, second, individuals who did not contract the disease. Materials and Methods: 66 individuals participated in the research, between 18 and 60 years old, being 33 people without contamination and 33 people who had a confirmed diagnosis. Participants answered two questionnaires: The Perceived Stress Questionnaire (QEP) to assess stress and another questionnaire to assess quality of life (SF-36). Results: The analysis of the results showed that the QEP of non-contaminated individuals was lower (0.64 + 0.04) than that of individuals who tested positive for the disease (0.73 + 0.02) (p = 0.0484), statistically significant difference, which shows that positive cases are perceived to be more stressed. In the analysis of SF-36, only the Functional Capacity domain showed statistically significant difference, and the volunteers who did not have the disease presented higher value (93.3 + 2.0) than contaminated individuals (73.0 + 3.6) (p < 0.0001), suggesting better quality of life. Conclusion: The study presents results that indicate the pandemic negatively impacted the health of the population, as participants diagnosed with COVID-19 had greater perceived stress and lower quality of life, compared to non-contaminated ones.

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Evapotranspirationand the estimation of crop coefficient (Kc) are essential information for irrigation management, contributing to increase the water use efficiency and optimizing resources, mainly using local data. This study aimed to determine the water consumption and the crop coefficient of cauliflower (Barcelona hybrid) in the northwestern region of Paraná. The experiment was conducted in a greenhouse at the Technical Irrigation Centerbelonging to the State University of Maringá, in Maringá -PR. The crop evapotranspiration was determined using constant water table lysimeters, and the reference evapotranspiration was estimated by the Penman-Monteith method (FAO), using climatological data collected in an automatic meteorological station. The cultivation was carried out from April to July. The total water consumption by the cauliflower was 215.11 mm, a higher value than that available in the FAO recommendations. The crop coefficient (Kc) recommended values for local conditions are 0.74 for the initial stage, 1.86 for the intermediate stage, and 1.50 for the final stage.

A evapotranspiraçãoe a estimativa dos coeficientes da cultura (Kc) são informações fundamentais para o manejo da irrigação, contribuindo para o aumento da eficiência no uso da água e otimização dos recursos, principalmente a utilizar dados locais. O trabalho teve como objetivo determinar o consumo hídrico e o coeficiente da cultura da couve-flor (híbrido Barcelona) na região noroeste do Paraná. O experimento foi conduzido em casa de vegetação no Centro Técnico de Irrigação pertencente à Universidade Estadual de Maringá, em Maringá -PR. A evapotranspiração da cultura foi determinada utilizando lisímetros de lençol freático constante, e a evapotranspiração de referência foi estimada pelo método de Penman-Monteith (FAO), a partir de informações climatológicas coletadas em estação meteorológica automática. O cultivo foi realizado no período de abril a julho. O consumo total de água pela cultura da couve-flor foi de 215,11 mm, sendo um valor maior do que o disponível nas recomendações da FAO. Os valores recomendados de coeficiente da cultura (Kc) para as condições de cultivo são de 0,74 para o estádio inicial, 1,86 para o estádio intermediário e 1,50 para o estádio final.

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The quality of cotton fiber is a fundamental criterion for determining the commercial value of the product, being influenced by the conditions of cultivation. The study aimed to evaluate irrigated and non-irrigated cultivation systems and the management of silicon fertilization on cotton fiber characteristics. The experiment was carried out in the field in Selvíria-MS, in the 2017/2018 agricultural crop. It was adopted a randomized block design, in a 2x6 factorial scheme, with two cultivationconditions (irrigated and rainfed) and six doses of Si (0, 50, 100, 150, 200 and 400 g ha-1), with four replications. Seeds of the genotype TMG 11 WS were used. Irrigation was carried out with sprinklers spaced at 6x6 m. The application of Si via leaf was carried out at 50 and 70 days after emergence. From thecotton fiber, the characteristics of length, micronaire, strength, uniformity, elongation, reflectance, yellowing, degree of leaves, impurity and impurity particles were analyzed. The data submitted to analysis of variance by the F test, the means compared for the cultivation condition, and regression analysis for the amount of Si. The cotton fiber in irrigated cultivation showed improvement in the characteristics resistance, reflectance and yellowing.The application of Si via leaf reduced the leaf grade in the cotton fibers.(AU)

A qualidade da fibra doalgodão é critério fundamental para determinação do valor comercial do produto, sendo influenciado pelas condições de cultivo. O estudo teve como objetivo avaliarsistemas de cultivo irrigado e não irrigado e o manejo da adubação de silício sobre características da fibra do algodão. O experimento foi desenvolvido a campo no município de Selvíria-MS, na safra 2017/2018. Foi adotado delineamento em blocos ao acaso, em esquema fatorial 2x6, sendo duas condições de cultivo (irrigado e sequeiro) e seis doses de Si (0, 50, 100, 150, 200 e 400 g ha-1), com quatro repetições. Foi utilizado sementes do genótipo TMG 11 WS. A irrigação foi realizada com aspersores espaçados em 6x6 m. A aplicação de Si via foliar foi realizado aos 50 e 70 dias após a emergência. A partir da fibra do algodão, foram analisadas as características de comprimento, micronaire, resistência, uniformidade, alongamento, reflectância, amarelecimento, graus de folhas, impureza e partículas de impureza.Os dados submetidos a análise de variância pelo teste F, as médias comparadas para a condição de cultivo, e análise de regressão para quantidade de Si. A fibra de algodão em cultivo irrigado apresentou melhoria nas características de resistência, reflectância e amarelamento. A aplicação de Si via foliar reduziu o grau de folhas nas fibras de algodão.(AU)

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Evapotranspirationand the estimation of crop coefficient (Kc) are essential information for irrigation management, contributing to increase the water use efficiency and optimizing resources, mainly using local data. This study aimed to determine the water consumption and the crop coefficient of cauliflower (Barcelona hybrid) in the northwestern region of Paraná. The experiment was conducted in a greenhouse at the Technical Irrigation Centerbelonging to the State University of Maringá, in Maringá -PR. The crop evapotranspiration was determined using constant water table lysimeters, and the reference evapotranspiration was estimated by the Penman-Monteith method (FAO), using climatological data collected in an automatic meteorological station. The cultivation was carried out from April to July. The total water consumption by the cauliflower was 215.11 mm, a higher value than that available in the FAO recommendations. The crop coefficient (Kc) recommended values for local conditions are 0.74 for the initial stage, 1.86 for the intermediate stage, and 1.50 for the final stage.(AU)

A evapotranspiraçãoe a estimativa dos coeficientes da cultura (Kc) são informações fundamentais para o manejo da irrigação, contribuindo para o aumento da eficiência no uso da água e otimização dos recursos, principalmente a utilizar dados locais. O trabalho teve como objetivo determinar o consumo hídrico e o coeficiente da cultura da couve-flor (híbrido Barcelona) na região noroeste do Paraná. O experimento foi conduzido em casa de vegetação no Centro Técnico de Irrigação pertencente à Universidade Estadual de Maringá, em Maringá -PR. A evapotranspiração da cultura foi determinada utilizando lisímetros de lençol freático constante, e a evapotranspiração de referência foi estimada pelo método de Penman-Monteith (FAO), a partir de informações climatológicas coletadas em estação meteorológica automática. O cultivo foi realizado no período de abril a julho. O consumo total de água pela cultura da couve-flor foi de 215,11 mm, sendo um valor maior do que o disponível nas recomendações da FAO. Os valores recomendados de coeficiente da cultura (Kc) para as condições de cultivo são de 0,74 para o estádio inicial, 1,86 para o estádio intermediário e 1,50 para o estádio final.(AU)

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BACKGROUND: HER2 over-expression is related with a poor prognosis in patients with invasive breast cancer tumors. Clinical associations have reported that somatic mutations of p53 more frequently detected in cases of sporadic breast cancer of the HER2 subtypes, besides a high percentage of HER2-amplifying tumors carry germline mutations of p53. The mechanisms responsible for the acquisition of oncogenic functions of p53 mutant proteins (mtp53), known as Gain of Function (GOF), over HER2 expression have not been reported. The objective of this study was to evaluate a possible relationship between p53 mutants and HER2 regulation. METHODS: HER2 expression (transcription and protein), as well as HER2 protein stabilization have been evaluated after inducing or silencing of p53 mutants' expression in cell lines. Finally, we evaluated the interaction of the p53 mutants over the HER2 receptor promoter. RESULTS: Higher HER2 expression in cell lines harboring endogenous mtp53 compared with wt or null expression of p53 cell lines. Transfection of p53 mutants (R248Q and R273C) in cell lines increased the expression of HER2. Silencing of p53 mutants, decrease HER2 expression. The p53 mutants R248Q and R273C significantly increase the luciferase activity on the HER2 promoter, and both mutants also promote acetylation of H3 and H4 histones binding in it. CONCLUSIONS: These findings show for the first time that p53 mutants induce over-expression of HER2 at transcriptional level of the HER2 protein. Our results could have clinical implications in breast cancer and other types of cancer where HER2 is over-expressed and used as a therapy target.

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Resveratrol (RES), a polyphenol compound with anti­proliferative properties, has been previously evaluated for its beneficial effects against a variety of tumour cells. The current study elucidated the means by which RES enhances the anti­proliferative effects of cisplatin (CIS) on MCF­7 cells, focusing on the inhibitory effects on DNA repair of double­strand breaks (DSBs). Chemoresistant MCF­7 cells (MCF­7R) were generated by continuous exposure to low concentrations of CIS (10 µM CIS­IC40) during 5 passages, with the IC50 value increasing ~3­fold. Using an MTT assay, we estimated the changes in IC50 for CIS in MCF­7, T47­D, MDA­MB­231 and MCF­7R cells in the presence of RES. The relative transcript level of Nbs­1, Mre­11 and Rad­50 genes was assessed using RT­qPCR analysis. Rad51 and H2AX [pSer139] protein expression was determined by western blot analysis. RES at 50 and 100 µM significantly enhanced the anti­proliferative effects of CIS in both MCF­7 and MCF­7R cells, decreasing the IC50 values for CIS to one­tenth and one­sixth, respectively. A total of 100 µM RES decreased the relative transcript levels of homologous recombination (HR) initiation complex components and the Rad51 protein level in MCF­7 and MCF­7R cells. After 48 h of CIS DNA damage, the levels of Rad51 protein increased, but this effect was inhibited by 100 µM RES. RES also maintained serine 139 phosphorylation of histone H2AX, suggesting that RES prevents the repair of DSBs. It was observed that RES exerts an antagonistic effect over CIS on the activation of Rad51 and sustained phosphorylation of H2AX. The results suggest that RES in combination with DNA damage­based therapy has potential as a strategy to overcome resistance and provide much safer and more effective treatment for breast cancer.

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This work describes the structure of the course "Pedagogical Training" introduced in the new Nursing Curriculum of Minas Gerais Federal University College of Nursing. We believe that attending this pedagogical course provides students with the opportunity to be the active subjects and architects of their own knowledge, which will make it possible for them to deliberately develop their roles as educators and perform in a critical manner in view of the reality of those involved. It can be said that pedagogical training constitutes one of the possibilities to think about, reflect on and construct the teaching-learning process during both the moments experienced in class and those in the fields of clinical practice.

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OBJECTIVE: To determine mortality risk factors for critically ill postoperative patients. PATIENTS: Two hundred eleven patients undergoing any type of surgery admitted to the intensive care unit (ICU) over a period of one year were enrolled. METHOD: We performed a cross-sectional study that was retrospective for pre- and intraoperative observations and prospective for ICU observations. A univariate analysis was performed and relative risk (95% CI) was calculated. Multivariate analysis was also performed and the adjusted odds ratio calculated (95% CI). The end point of measurement was death in the ICU. RESULTS: The independent risk factors for mortality were age 60 years (OR: 2.8; 95% CI 1.36-5.09), physical status ASA-V (OR: 9.66; 95% CI 5.62-14.31), intracranial surgery to treat severe head injury (OR: 5.33; 95% CI 3.08-9.47), hemorrhagic shock during surgery (OR: 5.20; 95% CI 4.16-8.29), arterial hypotension during surgery (OR: 4.0; 95% CI 2.78-11.65), APACHE III score upon admission to the ICU (OR: 9.29; 95% CI 3.62-21.38), multiple organ dysfunction syndrome (OR: 7.96; 95% CI 3.73-13.92), and cardiopulmonary arrest in the ICU (OR: 5.85; 95% CI 2.21-10.43). The APACHE III score demonstrated high sensitivity (87%) and positive predictive value (96%). CONCLUSIONS: Surgical patients in critical condition continue to account for a large number of admissions to and death in the ICU. The variables analyzed were sufficient to explain patient outcome, such that risk factors for mortality in surgical patients admitted to the ICU could be determined.

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Objetivo: Determinar los factores de riesgo de la mortalidad de los pacientes quirúrgicos graves.Material y métodos. Se incluyó a los 211 pacientes operados, independientemente del tipo de intervención quirúrgica practicada, que ingresaron durante el período de tiempo de un año en la unidad de cuidados intensivos.Se realizó un estudio de corte transversal, ambispectivo (retrospectivo en cuanto a las observaciones pre e intraoperatorias y prospectivo en cuanto a las observaciones en la UCI). Se analizaron variables correspondientes a las etapas pre, intra y postoperatorias. Se realizó un análisis univariado y se calculó el riesgo con un intervalo de confianza del 95 por ciento. También se realizó un análisis multivariado calculándose la odds ratio ajustada, así como los intervalos de confianza del 95 por ciento. El punto final de la medición fue la mortalidad en la UCI.Resultados. Las variables que constituyeron factores de riesgo independientes para la mortalidad fueron : edad igual o mayor de 60 años (OR: 2.8; 95 por ciento CI 1.36-5.09), estado físico 5, según la clasificación de la American Society of Anesthesiologists (OR: 9.66; 95 por ciento CI 5.62-14.31), cirugía intracraneal para tratamiento del trauma craneoencefálico grave (OR: 5.33; 95 por ciento CI 3.08-9.47), shock hemorrágico intraoperatorio (OR: 5.20; 95 por ciento CI 4.16-8.29), hipotensión arterial intraoperatoria (OR: 4.0; 95 por ciento CI 2.78-11.65), índice APACHE III en el momento del ingreso en UCI (OR: 9.29; 95 por ciento CI 3.62-21.38), síndrome de disfunción orgánica múltiple (OR: 7.96; 95 por ciento CI 3.73-13.92), y paro cardiorrespiratorio en la UCI (OR: 5.85; 95 por ciento CI 2.21-10.43). El índice APACHE III evidenció una elevada sensibilidad (87 por ciento) y valor predictivo positivo (96 por ciento).Conclusiones. Los pacientes quirúrgicos graves continúan siendo una causa importante de ingreso y mortalidad en la UCI(AU)

Objedtive: To determine mortality risk factors for critically ill postoperative patients. Paients: Two hundred eleven patients undergoing any type of surgery admitted to the intensive care unit (ICU) over a period of one year were enrolled. Method: We performed a cross-sectional study that was retrospective for pre- and intraoperative observations and prospective for ICU observations. A univariate analysis was performed and relative risk (95 percent CI) was calculated. Multivariate analysis was also performed and the adjusted odds ratio calculated (95 percent CI). The end point of measurement was death in the ICU. Resuls: The independent risk factors for mortality were age 60 years (OR: 2.8; 95 percent CI 1.36-5.09), physical status ASA-V (OR: 9.66; 95 percent CI 5.62-14.31), intracranial surgery to treat severe head injury (OR: 5.33; 95 percent CI 3.08-9.47), hemorrhagic shock during surgery (OR: 5.20; 95 percent CI 4.16-8.29), arterial hypotension during surgery (OR: 4.0; 95 percent CI 2.78-11.65), APACHE III score upon admission to the ICU (OR: 9.29; 95 percent CI 3.62-21.38), multiple organ dysfunction syndrome (OR: 7.96; 95 percent CI 3.73-13.92), and cardiopulmonary arrest in the ICU (OR: 5.85; 95 percent CI 2.21-10.43). The APACHE III score demonstrated high sensitivity (87 percent) and positive predictive value (96 percent). Conclusions: Surgical patients in critical condition continue to account for a large number of admissions to and death in the ICU. The variables analyzed were sufficient to explain patient outcome, such that risk factors for mortality in surgical patients admitted to the ICU could be determined(AU)

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This paper describes a teaching experience in psychiatric nursing at an open mental health care service. Students' discourses show how meaningful and rich an experience it has been for their careers. Participating in this training has allowed them to learn the role of nurses, to improve assistance by applying both theory and practice as well as to recognize people with mental disorders as ordinary citizens.

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The product of the p53 tumor suppressor gene has been implicated in safeguarding genomic stability by transactivating genes involved in cell cycle arrest, repair of DNA damage or induction of apoptosis. Several properties of p53 suggest that it might be directly involved in DNA repair processes. Eukaryotic DNA is highly organized in supercoiled loops anchored to the nuclear matrix. This organization is very important for cell function and survival, suggesting that repair of DNA damage must include both, the integrity of the double helix and the complex DNA topology. In this work, we studied the kinetics and efficiency of higher-order DNA structure repair in cells with normal and reduced levels of p53, and present evidence suggesting that p53 may be involved in the stabilization and/or repair of higher-order DNA structure.

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