RESUMO
This paper describes the development and evaluation of a HPLC and UV spectrophotometric methods to quantify cefuroxime sodium in injectables. HPLC analysis were carried out using a C18 Wat 054275 column and a mobile phase composed of methanol and water (70:30), with a flow rate of 0.8 mL/min and UV detection at 280 nm. For the spectrophotometric analysis, water was used as solvent and the wavelength of 280 nm was selected for the detection. Both methods were found to quantify cefuroxime sodium in injectables accurately. Therefore HPLC and UV methods presented the most reliable results for the analyses of injectables.
Assuntos
Cefuroxima/análise , Cromatografia Líquida de Alta Pressão/métodos , Preparações Farmacêuticas/análise , Espectrofotometria Ultravioleta/métodosRESUMO
The aims of this study were to evaluate the effects of light and temperature on seed germination and the effect of light on seedling emergence of Clausena excavata, an invasive exotic species. Seeds were incubated at constant temperatures (10 to 40 ºC), under continuous white light and darkness. Seedling emergence was evaluated under full sun and canopy shade. There was no significant difference between seeds germinated in the presence or absence of light at temperatures between 20 and 35 ºC. Seeds maintained at 20 ºC germinated more slowly than other temperatures. Independent of temperature, seed germination was much more synchronised under white light than under darkness. In the field, emergence occurred in both environments, but a greater percentage was observed under full sun. Seedling emergence occurred at the same rate and synchronisation index under both conditions. C. excavata has a high invader potential in open areas and shaded environments.